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Download Anti-SPRR1a antibody ab125374 Product datasheet 1 Abreviews 2 Images
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Product datasheet Anti-SPRR1a antibody ab125374 1 Abreviews 2 Images Overview Product name Anti-SPRR1a antibody Description Rabbit polyclonal to SPRR1a Tested applications IHC-Fr, WB Species reactivity Reacts with: Human Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Cat, Pig Immunogen Synthetic peptide corresponding to a region within internal amino acids 40-89 (PCHPKVPEPC HPKVPEPCQP KVPEPCQPKV PEPCPSTVTP APAQQKTKQK) of Human SPRR1a (NP_005978). Run BLAST with Positive control Run BLAST with HT1080 cell lysate Properties Form Liquid Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. Storage buffer Constituents: 97% PBS, 2% Sucrose Purity Immunogen affinity purified Clonality Polyclonal Isotype IgG Applications Our Abpromise guarantee covers the use of ab125374 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application Abreviews Notes IHC-Fr Use at an assay dependent concentration. WB Use a concentration of 1 µg/ml. Predicted molecular weight: 10 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. Target 1 Relevance The ability of neurons to regenerate an axon after injury is determined by both the surrounding environment and factors intrinsic to the damaged neuron. Neuronal SPRR1A is likely a significant contributor to successful nerve regeneration. The small proline-rich repeat protein 1A (SPRR1A) is not detectable in uninjured neurons but is induced by >60-fold after peripheral axonal damage being localized to injured neurons and axons. SPRR1A is one of a group of epithelial differentiation genes, including s100c and p21/waf, that are cocurrently induced in neurons by axotomy. Overexpressed SPRR1A colocalizes with F-actin in membrane ruffles and augments axonal outgrowth on a range of substrates. In axotomized sensory neurons, reduction of SPRR1A function restricts axonal outgrowth. Cellular localization Cytoplasmic Anti-SPRR1a antibody images Anti-SPRR1a antibody (ab125374) at 1 µg/ml + HT1080 cell lysate at 10 µg Predicted band size : 10 kDa Western blot - Anti-SPRR1a antibody (ab125374) ab125374 staining SPRR1a in Human skin tissue by Immunohistochemistry (Frozen sections). Tissue was fixed with acetone and blocked with 1% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/300 in 1% BSA + 10% goat serum) for 16 hours at 4°C. An AlexaFluor®546-conjugated goat Immunohistochemistry (Frozen sections) - Anti- anti-rabbit polyclonal IgG (1/1000) was used SPRR1a antibody (ab125374) as the secondary antibody. Nuclei were This image is courtesy of an Abreview submitted by Yuan Cao stained with DAPI (blue). Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish 2 Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors 3