Download Anti-SPRR1a antibody ab125374 Product datasheet 1 Abreviews 2 Images

Product datasheet
Anti-SPRR1a antibody ab125374
1 Abreviews 2 Images
Overview
Product name
Anti-SPRR1a antibody
Description
Rabbit polyclonal to SPRR1a
Tested applications
IHC-Fr, WB
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Cat, Pig
Immunogen
Synthetic peptide corresponding to a region within internal amino acids 40-89 (PCHPKVPEPC
HPKVPEPCQP KVPEPCQPKV PEPCPSTVTP APAQQKTKQK) of Human SPRR1a
(NP_005978).
Run BLAST with
Positive control
Run BLAST with
HT1080 cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Constituents: 97% PBS, 2% Sucrose
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab125374 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
IHC-Fr
Use at an assay dependent concentration.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 10 kDa. Good
results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Target
1
Relevance
The ability of neurons to regenerate an axon after injury is determined by both the surrounding
environment and factors intrinsic to the damaged neuron. Neuronal SPRR1A is likely a
significant contributor to successful nerve regeneration. The small proline-rich repeat protein 1A
(SPRR1A) is not detectable in uninjured neurons but is induced by >60-fold after peripheral
axonal damage being localized to injured neurons and axons. SPRR1A is one of a group of
epithelial differentiation genes, including s100c and p21/waf, that are cocurrently induced in
neurons by axotomy. Overexpressed SPRR1A colocalizes with F-actin in membrane ruffles and
augments axonal outgrowth on a range of substrates. In axotomized sensory neurons, reduction
of SPRR1A function restricts axonal outgrowth.
Cellular localization
Cytoplasmic
Anti-SPRR1a antibody images
Anti-SPRR1a antibody (ab125374) at 1 µg/ml
+ HT1080 cell lysate at 10 µg
Predicted band size : 10 kDa
Western blot - Anti-SPRR1a antibody (ab125374)
ab125374 staining SPRR1a in Human skin
tissue by Immunohistochemistry (Frozen
sections).
Tissue was fixed with acetone and blocked
with 1% BSA for 1 hour at 22°C. Samples
were incubated with primary antibody (1/300
in 1% BSA + 10% goat serum) for 16 hours at
4°C. An AlexaFluor®546-conjugated goat
Immunohistochemistry (Frozen sections) - Anti-
anti-rabbit polyclonal IgG (1/1000) was used
SPRR1a antibody (ab125374)
as the secondary antibody. Nuclei were
This image is courtesy of an Abreview submitted by
Yuan Cao
stained with DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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