* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download Unit1-Probesweb
DNA methylation wikipedia , lookup
Metagenomics wikipedia , lookup
Epigenetic clock wikipedia , lookup
DNA sequencing wikipedia , lookup
Human genome wikipedia , lookup
DNA barcoding wikipedia , lookup
Mitochondrial DNA wikipedia , lookup
Epigenetics wikipedia , lookup
Genetic engineering wikipedia , lookup
Zinc finger nuclease wikipedia , lookup
Designer baby wikipedia , lookup
Genomic library wikipedia , lookup
Nutriepigenomics wikipedia , lookup
DNA profiling wikipedia , lookup
DNA polymerase wikipedia , lookup
Site-specific recombinase technology wikipedia , lookup
No-SCAR (Scarless Cas9 Assisted Recombineering) Genome Editing wikipedia , lookup
Point mutation wikipedia , lookup
Cancer epigenetics wikipedia , lookup
Comparative genomic hybridization wikipedia , lookup
Primary transcript wikipedia , lookup
Genome editing wikipedia , lookup
Microevolution wikipedia , lookup
Molecular Inversion Probe wikipedia , lookup
Bisulfite sequencing wikipedia , lookup
DNA damage theory of aging wikipedia , lookup
Gel electrophoresis of nucleic acids wikipedia , lookup
Genealogical DNA test wikipedia , lookup
Microsatellite wikipedia , lookup
SNP genotyping wikipedia , lookup
Vectors in gene therapy wikipedia , lookup
United Kingdom National DNA Database wikipedia , lookup
DNA vaccination wikipedia , lookup
Nucleic acid analogue wikipedia , lookup
Molecular cloning wikipedia , lookup
Non-coding DNA wikipedia , lookup
Epigenomics wikipedia , lookup
Cre-Lox recombination wikipedia , lookup
Therapeutic gene modulation wikipedia , lookup
Nucleic acid double helix wikipedia , lookup
Artificial gene synthesis wikipedia , lookup
Extrachromosomal DNA wikipedia , lookup
DNA supercoil wikipedia , lookup
History of genetic engineering wikipedia , lookup
Cell-free fetal DNA wikipedia , lookup
Detecting DNA with DNA probes arrays • DNA sequences can be detected by DNA probes and arrays (= collection of microscopic DNA spots attached to a solid surface) • DNA probes are pieces of DNA used to detect the presence of specific sequences in DNA. (Do not get probes mixed up with the primers used in PCR.) • What characteristics will DNA probes need to have? DNA probes are: • • • • Made of DNA Short Single stranded Complementary to the sequence being searched for • Labelled with fluorescent or radioactive markers to allow detection Example 1: Using DNA probes to search a match on an organism’s DNA (N.b. 1: This is not an array N.b. 2: This corresponds to the example in the printed notes) Example 2: using DNA probes for microarrays (see book p74) (array= collection of microscopic DNA spots attached to a solid surface) Microarray Small sections of genomic DNA as microscopic spots fixed onto a surface as an orderly collection Microarrays can be used to study the expression of genes and compare patterns between healthy and unhealthy cells. It is the mRNA from cells which is used to form labelled probes (after it has been copied into single stranded DNA). (To compare gene expression in healthy and diseased cells) Fluorescent labeling (To obtain DNA from the RNA because it will hybridize better to the DNA on the array (To be able to see When the DNA has hybridized to the DNA fixed on the array) • DNA probes can be used to detect: – Single gene mutations – Changes in gene expression due to disease (microarray) • Questions: • Booklet p15: 1 • Book q 2&3 p76 + What you should know.