* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download Nucleic Acids 101 Last week`s grand challenge
Oncogenomics wikipedia , lookup
Frameshift mutation wikipedia , lookup
Metagenomics wikipedia , lookup
DNA barcoding wikipedia , lookup
Epigenetics of neurodegenerative diseases wikipedia , lookup
Mitochondrial DNA wikipedia , lookup
Gene therapy wikipedia , lookup
Comparative genomic hybridization wikipedia , lookup
Human genome wikipedia , lookup
Genome evolution wikipedia , lookup
Public health genomics wikipedia , lookup
DNA profiling wikipedia , lookup
Primary transcript wikipedia , lookup
Genome (book) wikipedia , lookup
DNA polymerase wikipedia , lookup
Zinc finger nuclease wikipedia , lookup
Bisulfite sequencing wikipedia , lookup
DNA vaccination wikipedia , lookup
Nutriepigenomics wikipedia , lookup
Genetic engineering wikipedia , lookup
Cancer epigenetics wikipedia , lookup
No-SCAR (Scarless Cas9 Assisted Recombineering) Genome Editing wikipedia , lookup
DNA damage theory of aging wikipedia , lookup
United Kingdom National DNA Database wikipedia , lookup
Gel electrophoresis of nucleic acids wikipedia , lookup
SNP genotyping wikipedia , lookup
Genomic library wikipedia , lookup
Site-specific recombinase technology wikipedia , lookup
Point mutation wikipedia , lookup
Genealogical DNA test wikipedia , lookup
Extrachromosomal DNA wikipedia , lookup
Molecular cloning wikipedia , lookup
Epigenomics wikipedia , lookup
Nucleic acid analogue wikipedia , lookup
Nucleic acid double helix wikipedia , lookup
Cell-free fetal DNA wikipedia , lookup
Cre-Lox recombination wikipedia , lookup
Microsatellite wikipedia , lookup
Vectors in gene therapy wikipedia , lookup
Non-coding DNA wikipedia , lookup
DNA supercoil wikipedia , lookup
Designer baby wikipedia , lookup
Deoxyribozyme wikipedia , lookup
Therapeutic gene modulation wikipedia , lookup
Microevolution wikipedia , lookup
Genome editing wikipedia , lookup
History of genetic engineering wikipedia , lookup
Nucleic Acids 101 Last week’s grand challenge: Reading and interpreting genetic information DNA Repair: -7 DNA polymerase 10 -9 Mismatch repair 10 Genomics Restriction Nucleases Ligation Grand challenge: Every disease requires a tremendous amount of interdisciplinary effort to understand and fight it. Can this be streamlined? The frequency of crossing-over is ~proportional to the physical distance between them. Find DNA markers close to and flanking the gene of interest Positional Cloning • A chromosome has a single DNA molecule running the length of the chromosome • Map the gene for the trait in question in that DNA molecule; identify close flanking markers • “Walk” between the flanking markers • Identify the gene in question between the markers How do you map a gene? • Collect DNA samples from family members in a pedigree • Seek linkage to a polymorphic DNA marker • Take advantage of the Human Genome Project to identify the chromosomal region • Narrow down the location in the overlapping clones What are “DNA markers”? • RFLP--Restriction Fragment Length Polyhmorphism • SNP--Single Nucleotide Polymorphisms Mutation destroys a restriction site, causing a RFLP (This is also a SNP--Single Nucleotide Polymorphism) RFLP STRP or VNTR Pedigree of Huntington Disease SNP’s: Single Nucleotide Polymorphisms NEXT CLASS Central Dogma