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Chapter 19 1. Good cloning vectors must possess all but which of the following qualities? A. They should possess their own origin of replication B. They should be readily accepted by cloning hosts C. They should be easily manipulated D. They should be capable of carrying a significant piece of donor DNA E. They should be resistant to restriction endonucleases 2. The insertion of a cloning vector into a cloning host typically involves what process? A. Transduction B. Polymerase chain reaction C. Transformation D. Hybridization E. Conjugation 3. DNA fragments that are 500bp, 1000bp, and 2000bp in length are separated by gel electrophoresis. Diagram a gel including electric charge, and labeled fragments. 4. PCR is a process used to clone a specific fragment of DNA. What are the 4 main components in a PCR and what are their purposes? 1. 2. 3. 4. 5. Oligonucleotide gene probes are defined as what? A. Enzymes that recognize and subsequently degrade foreign DNA B. The pieces of DNA produced by restriction endonucleases C. An enzyme important in splicing genes into plasmids and chromosomes D. A short stretch of DNA of a known sequence that will base-pair with a complementary sequence E. A piece of DNA to which new nucleotides are added during DNA sequencing 6. What is the function of reverse transcriptase in making a cDNA library? 7. In Sanger sequencing, ddNTPs are used for what function?