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Unit 6 Review Game 1 DNA polymerase and RNA polymerase can only move along the template DNA strand in the _____ direction. A 1' to 5' B 2' to 3' C 3' to 5' D 5' to 1' 2 In this diagram of a nucleotide, the letter "D" indicates a A ribose sugar. B phosphate group. C nitrogenous base. D deoxyribose sugar. 3 RNA nucleotides and DNA nucleotides differ in the A location of their phosphate groups. B number of carbon atoms in their sugars. C nitrogen base that hydrogen bonds with adenine. D nitrogen base that hydrogen bonds with cytosine. 4 The last step A B C D in the replication of DNA is catalyzed by ligase. primase. helicase. DNA polymerase. 5 Which of the following statements about DNA replication is FALSE? A Each replication bubble has one replication fork. B RNA primers are constructed by the enzyme primase. C DNA polymerase can only assemble new DNA strands in the 5' to 3' direction. D DNA polymerase can only move along the template DNA strand in the 3' to 5' direction. 6 In the accompanying image, the enzyme helicase is indicated by the letter _____. A A B B C C D D E E 7 RNA processing is the process in which pre-mRNA is edited into mRNA. RNA processing removes ______ and splices together _______. A introns ... exons B uracil ... thymine C polypeptides ... proteins D RNA primers ... Okazaki fragments 8 Small nuclear ribonucleoproteins (snRNPs) and other proteins combine to make up the A lysosome. B ribosome. C spliceosome. D centrosome. 9 During translation, when the ribosome reaches a stop codon, the releasing factor attaches at the ribosome's ___ site. A A B E C P D Q 10 The single A B C D strand in the bottom half of this diagram of transcription represents DNA. RNA. the promoter. RNA polymerase. 11 An aminoacyl-tRNA synthetase attaches a(n) ______ to a(n) ______. A thymine ... uracil B adenine ... mRNA C amino acid ... tRNA D nucleic acid ... ribosome 12 The tRNA anticodon CUG is complementary to the mRNA codon ____, which was originally transcribed from the DNA codon ____. A GGC ... CGG B CTG ... GAC C GAC ... CTG D GTC ... CAG 13 During RNA A B C D processing, a(n) _____ is added to the 5' end of the RNA and a _____ is added to the 3' end. modified guanine nucleotide ... poly-C tail modified guanine nucleotide ... poly-A tail poly-A tail ... modified adenine nucleotide poly-U tail ... modified thymine nucleotide 14 What name is given to the process which involves the small subunit of the ribosome attaching to the mRNA? A elongation B initiation (of translation) C initiation (of transcription) D termination (of translation) 15 As a result A B C D of the lysogenic cycle of bacteriophage reproduction, a prophage is created. the host cell is destroyed. viral ribosomes are produced. the host cell's DNA is destroyed. 16 Which of the following statements about retroviruses is TRUE? A All retroviruses have double-stranded RNA genomes. B Retroviruses can only replicate through the lysogenic cycle. C Retroviruses are usually larger than the host cells they infect. D Retroviruses have a single-stranded RNA genome that acts as a template for DNA synthesis. 17 The trp operon is a repressible operon, in which tryptophan acts as a corepressor. This means that in the presence of tryptophan, the trp operon is A turned on. B transcribed. C only transcribed at the basal level. D transcribed at a faster than usual rate. 18 In this diagram of chromatin structure, the letter B indicates A histones. B supercoils. C a nucleosome. D a DNA double helix. 19 Which of the following statements about prokaryotic vs. eukaryotic genetic material is FALSE? A Both genomes have introns. B Both genomes consist of a combination of DNA and proteins. C Prokaryotes have less noncoding DNA than eukaryotes. D Both prokaryotes and eukaryotes use RNA polymerase in the process of transcription. 20 Retrotransposons are genetic elements that can amplify themselves in a genome by a "copy and paste" method. This method involves the translation of the RNA transcript of the transposon to produce an enzyme called "__________" that converts the RNA back to DNA. A DNA ligase B RNA polymerase C reverse transcriptase D restriction endonuclease 21 Which of the following is LEAST likely to increase the rate of transcription of a particular segment of DNA? A the acetylation of histones B the methylation of DNA nucleotides C the conversion of heterochromatin to euchromatin D the binding of activator proteins to enhancer regions of DNA 22 The inheritance of traits transmitted by mechanisms not directly involving the nucleotide sequence is called "epigenetics." For instance, although these mice share identical DNA, they have distinct coat colors and body compositions. Typically, this occurs when environmental factors affect A DNA sequence. B gene expression. C signal transduction. D cell-to-cell communication. 23 Prokaryotic genomes lack all of the following EXCEPT A introns. B histones. C nucleosomes. D deoxyribonucleic acid. 24 In this illustration of the various mechanisms of gene regulation, the process of translation is indicated by the letter ____. A A B B C C D D E E 25 The expression of genes in eukaryotes usually involves factors that can affect all of the following EXCEPT A mRNA degradation. B protein degradation. C enzyme modification of chromatin. D binding of a repressor to an operator. 26 In nature, known as A B C D restriction enzymes are used by bacteria to cut foreign DNA into pieces. Restriction enzymes are also ligases. helicases. polymerases. endonucleases. 27 In our bacterial transformation lab, the function of arabinose was to inactivate the repressor protein that normally inhibits the transcription of the pGLO genes, allowing the expression of the pGLO protein. Therefore, in this bioengineered system, arabinose acts as a(n) A inducer. B operator. C enhancer. D corepressor. 28 Restriction enzymes are used in recombinant DNA technology to create restriction fragments that can then be A separated by gel electrophoresis. B specifically identified using the Southern blotting technique. C used in RFLP analysis to identify a mutation in the DNA sequence at a particular restriction site. D All of the above 29 The rate at which a DNA fragment moves through a gel during electrophoresis can be affected by A increasing the size of the DNA fragment. B neutralizing the negative charges within the DNA fragment. C increasing the density of the gel through which the fragment moves. D All of the above 30 Plasmid vectors can be used to insert human genes into bacteria so that they produce human proteins. In general, however, it is not possible to directly insert a eukaryotic gene into a bacterium and get it to express the desired eukaryotic protein. Why? A Bacteria do not undergo translation. B Bacteria do not undergo RNA processing. C Bacteria use a different genetic code than humans. D Bacteria use different base-pairing rules in their DNA. Answer Key : Unit 6 Review Game Question: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 Answer C D C A A B A C A B C C B B A D C C A C B B D D D D A D D B