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Quote of the Day! 0 “For every fact there is an infinity of hypotheses.” 0 ~Robert M. Pirsig Objectives 0 The Student will be able to: 0 1.) Describe the process of how DNA replicates itself. 0 2.) Model the process of DNA replication. Recall 0 Where does DNA Replication occur? 0 Why is DNA replication important? 0 What can happen if DNA replication goes wrong? Let’s Make a Model 0 At your table you have many pieces of the DNA molecule. With a partner construct a DNA molecule with 4 base pairs (doesn’t matter which ones). 0 Must have a base, ribose and a phosphate group. If you were in charge…. 0 How would you start to replication the DNA in front of you? How it’s really done. 0 Step 1 of DNA Replication: 0 An enzyme called “Helicase,” comes in and breaks the hydrogen bonds pulling the two DNA strands apart. 0 The separated DNA molecule is called a “Replication fork.” 0 Create a “Helicase” on a sheet of paper and show this step with you desk models. What does this look like? Replication Fork Step 2 of DNA Replication 0 Another enzyme called “DNA Polymerase I,” attaches itself to one of the separated strands of DNA. 0 Once attached DNA Polymerase I starts to copy the DNA strand by attaching the missing parts of the base pair. 0 The strand that DNA Polymerase I attaches to is called the “Leading strand.” What does this look like? 0 Model this step with your partner, create a DNA Polymerase I on a separate sheet of paper to use in this step. What should this look like? Step 3 of DNA Replication 0 The other strand is then copied by yet another enzyme called “DNA Polymerase II,” this adds the complimentary base pair. 0 The strand that DNA Polymerase II attaches to is called the “Lagging Strand.” 0 Create a DNA polymerase II on a piece of paper and model this with your partner. Step 4 of DNA Replication 0 Once DNA Polymerase I and II are finished copying the Leading and Lagging strands two complete strands of DNA separate from one another. 0 When the two DNA molecules separate a molecule called Telomeres attach to the ends of the DNA and cap them to ensure the Bases don’t shift around. 0 Create Telomeres out of paper and model this step. What this step looks like with when finished Step 5 of DNA Replication 0 Enzymes called nucleases come in and double check that the DNA was copied properly. 0 If the nucleases find and error they either fix the problem directly or have other cell machinery to do so. 0 What could’ve gone wrong in this process? Review and Assess 0 With your notes and your partner, conduct this entire process of start to finish.