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Topic 4 Jeopardy! Replication Photosynth esis Transcription/ Translation SL DNA / RNA Protein Mixture II Q $100 Q $100 Q $100 Q $100 Q $100 Q $200 Q $200 Q $200 Q $200 Q $200 Q $300 Q $300 Q $300 Q $300 Q $300 Q $400 Q $400 Q $400 Q $400 Q $400 Q $500 Q $500 Q $500 Q $500 Q $500 Genetics SL $100 Question Replication What happens during the formation of Okazaki fragments? A.DNA polymerase III adds nucleotides in the 3´ → 5´ direction. B.DNA polymerase III adds nucleotides in the 5´ → 3´ direction. C. DNA polymerase I adds nucleotides in the 5´ → 3´ direction. D. RNA polymerase adds nucleotides in the 3´ → 5´ direction $100 Answer Replication What happens during the formation of Okazaki fragments? B.DNA polymerase III adds nucleotides in the 5´ → 3´ direction. $200 Question Replication Where would DNA polymerase link the next nucleotide during replication? $200 Answer Replication I $300 Question Replication Which enzyme removes the RNA primer during replication? $300 Answer Replication DNA Polymerase I $400 Question Replication Draw & Label two nucleotides in double complementary strands Label all components of one nucleotide (3 things) Label all important bonds (3 bonds) $400 Answer Replication $500 Question Replication The following diagram represents replication in DNA. A B State the name and describe the function for the enzymes labelled A and B on the diagram $500 Answer Photosynthesis A is DNA Polymerase III B is Helicase A B $100 Question Photosynthesis Identify the structures label I & IV and state their function $100 Answer from Photosynthesis I is the thylakoid membrane – location of light dependent reaction/ Electron Transport Chain and chemiosmosis/ oxidative phosphorylation IV is the Stroma –location of the Calvin Cycle/ Light Independent reaction $200 Question from Photosynthesis In the graph below which line shows an action spectrum of photosynthesis? A D B C Relative rate of photosynthesis 400 500 600 Wavelength / nm 700 $200 Answer from Photosynthesis A A D B C Relative rate of photosynthesis 400 500 600 Wavelength / nm 700 $300 Question from Photosynthesis Identify three Products of the light dependent reaction $300 Answer from Photosynthesis NADPH + ATP OXYGEN GAS HYDROGENS $400 Question from Photosynthesis The diagram below shows the light independent stage of photosynthesis (the Calvin Cycle). Where (I,II, III, IV) is energy, from ATP generated during the light dependent stage, used in this Glucose Ribulose cycle? bisphosphate I (RuBP) CO2 II Triose Phosphate (TP) Glycerate 3–Phosphate (GP) III $400 Answer from Photosynthesis III, I Glucose I Ribulose bisphosphate (RuBP) CO2 II Triose Phosphate (TP) Glycerate 3–Phosphate (GP) III $500 Photosynthesis Draw a line on the graph below to show the relationship between light intensity and the rate of photosynthesis. (2) And identify two things that would be not produced in low light intensity during the Light Dependent reaction that would affect the Calvin Cycle. $500 Answer from Photosynthesis NADPH & ATP $100 Question Transcription/ Translation What is a difference between the sense and antisense strands of DNA? A. Nucleotides are linked to the sense strand by hydrogen bonding during transcription, but not to the antisense strand. B. The sense strand has the same base sequence as tRNA, but the antisense strand does not. C. Nucleotides are linked to the antisense strand by hydrogen bonding during transcription, but not to the sense strand. D. The antisense strand has the same base sequence as mRNA but the sense strand does not. $100 Answer from DNA Transcription/ Translation What is a difference between the sense and antisense strands of DNA? C. Nucleotides are linked to the antisense strand by hydrogen bonding during transcription, but not to the sense strand. $200 Question from Mixture The drawing below shows a short section of a DNA molecule. What is labelled by I, II and III? A. B. C. D. I 3′ end 5′ end 3′ end 5′ end II purine pyrimidine pyrimidine purine III hydrogen bond covalent bond hydrogen bond covalent bond I II III $200 Answer from Mixture D. I II 5′ end purine III covalent bond I II III $300 Question from mixture To which parts of the deoxyribose molecule do phosphates bind in DNA? V CH2 OH I O H IV H H H OH II OH III H $300 Answer from Mixture III and V V CH2 OH I O H IV H H H OH II OH III H $400 Question Mixture Transcribe the below gene AAT TCG CTA GGC AAT $400 Answer from Mixture Transcribe the below gene AAT TCG CTA GGC AAT UUA UGC GAU CCG UUA $500 Question from Mixture Translate the below gene TAC CCA TA G C A C G T T A C A $500 Answer from 4.3 Monohybrid Translate the below gene TAC CCA TA G C A C G T T A C A AUG GGU AUC GUG CAA UGU Methionine—Glycine—Isoleucine –Valine – Glutamine -- Threonine $100 Question from 3.3 DNA / RNA /Protein In the Meselson-Stahl DNA replication experiment, what percent of the DNA was composed of one light strand and one heavy strand after one generation of growth in 14N containing growth media? $100 Answer 3.3 DNA / RNA /Protein 100 % $200 Question 3.3 DNA/ RNA/ PROTEIN Which enzyme catalyzes the elongation of the leading strand? $200 Answer 3.3 DNA/ RNA/ Protein DNA PolymeraseIII $300 Question DNA/ RNA/ Protein A certain gene in a bacterium codes for a polypeptide that is 120 amino acids long. How many nucleotides are needed in the mRNA to code for this polypeptide? $300 Answer DNA/ RNA/ Protein 360 nucleotides $400 Question DNA/ RNA/ Protein State the type of bonds that (i) connect base pairs in a DNA molecule. ................................................................................. (ii) link DNA nucleotides into a single strand. ....................................................................................... $400 Answer from DNA/ RNA/ PROTEINS State the type of bonds that (i)connect base pairs in a DNA molecule. HYDROGEN BONDS (ii)link DNA nucleotides into a single strand. COVALENT BONDS $500 Question DNA/ mRNA State three differences between RNA & DNA $500 Answer DNA/RNA DNA Double Stranded Deoxyribose sugar Thymine nitrogenous base RNA Single Stranded Ribose sugar Uracil Nitrogenous Base $100 Question Mixture II In RNA editing by the splicesomes what is removed from the RNA sequence $100 Answer Mixture II INTRONS– Parts of DNA that were transcribed into mRNA that do not Code for anything. $200 Question Mixture II If mRNA has a codon CAU, what is the corresponding anticodon on the tRNA molecule and what amino acid does the t-RNA have bond to it. $200 Answer Mixture II ?????? $300 QUESTION Mixture II The diagram below shows the structure of a ribosome during protein synthesis. II polypeptide State the names of the structures labelled I, II, III, IV. I III IV $300 Answer MIXTURE II polypeptide I. Small Ribosome subunit II. t-RNA I III IV III. Large Ribosome subunit IV. mRNA Daily Double! $400 Question Mixture II A tRNA molecule with carries the amino acid valine. Identify all the possible anticodons the t-RNA molecule may have. $400 Answer Mixture II ???? $500 Question Mixture In the Hershey & Chase Experiment, • Bacteriophages/ viruses were grown in a medium containing radioactive Sulfur-34, • and then these viruses were used to infect bacteria. • The contents of the infected bacteria and viruses were in an aqueous environment, and where then placed in a test tube. • This test tube was blended and centrifuge. • The bacteria cells settled at the bottom of a test tube in a pellet, while the rest of the liquid contents floated above the bacteria pellet Where in the test tube is the radioactive Sulfur-34 and explain how you know? $500 Answer Mixture Sulfur- 34 is found in the liquid content above the bacteria pellet. It is not part of the bacteria pellet, because Sulfur-34 would be part of the protein coat of the bacteriophage/ virus which is not injected into the bacteria during infection. During infection DNA is injected into the bacteria from the virus (bacteriophage), because DNA is the genetic material & DNA does not contain Sulfur atoms. FINAL JEAPARDY Topic 2.6/2.7 DNA & RNA FINAL JEAPARDY Draw 4 nucleotides in a single strand of DNA. Label all parts of one nucleotide (3) Label 3 bonds between different molecules. Be sure to state whether they are covalent, ionic or hydrogen bonds. OLD BIBILICAL STORY –made into a movie(Older than Ms. Tyler) The story was recently remade into another movie, but with a different name. American Movie --Recently released Drama Based on a non-fiction best-selling novel American Drama Movie --Recently released American Movie Recently released Drama Nominated for many awards Boyhood American Movie Science fantasy Almost as Old as Ms. Tyler Many sequels/ prequels New one will be released soon. Star Wars– Episode 4 – The original