PCR and qPCR product guide
... not required during each cycle. Taq polymerase was commercialized in the late 1980s, spurring a boom in PCR and ultimately becoming Science magazine’s first “molecule of the year” in 19895. Although a vast improvement over using E. coli Pol I, Taq polymerase still had some serious drawbacks. Limited ...
... not required during each cycle. Taq polymerase was commercialized in the late 1980s, spurring a boom in PCR and ultimately becoming Science magazine’s first “molecule of the year” in 19895. Although a vast improvement over using E. coli Pol I, Taq polymerase still had some serious drawbacks. Limited ...
Peptide nucleic acids 2
... As mentioned above, the preparation of PNA oligomers is based on standard solid phase peptide synthesis protocols. Two different protection schemes have been employed, both providing oligomers in high yields and purity. In the strategy first reported, the tert-butyloxycarbonyl (Boc) group was chosen ...
... As mentioned above, the preparation of PNA oligomers is based on standard solid phase peptide synthesis protocols. Two different protection schemes have been employed, both providing oligomers in high yields and purity. In the strategy first reported, the tert-butyloxycarbonyl (Boc) group was chosen ...
PO 4
... carbon atoms in its molecule Examples:mRNA, tRNA, and rRNA Deoxyribose is almost the same but lacks one oxygen atom Example:DNA Both molecules may be represented by the symbol ...
... carbon atoms in its molecule Examples:mRNA, tRNA, and rRNA Deoxyribose is almost the same but lacks one oxygen atom Example:DNA Both molecules may be represented by the symbol ...
Redesign of the monomer–monomer interface of Cre recombinase
... knockouts, where a gene of interest is flanked by loxP sites (‘floxed’) (2). Expression of Cre recombinase under the control of promoters that are specific for particular tissues or developmental stages abrogates gene function by physical excision from the genome. The utility of this system depends ...
... knockouts, where a gene of interest is flanked by loxP sites (‘floxed’) (2). Expression of Cre recombinase under the control of promoters that are specific for particular tissues or developmental stages abrogates gene function by physical excision from the genome. The utility of this system depends ...
SURVEY AND SUMMARY New insights into Hoogsteen base pairs
... HG bps help avoid a steric clash between the guanine exocyclic NH2 group and a nearby leucine side chain (16). Two consecutive A•T HG bps in X-ray structures of DNA in complex with the tumor suppressor protein p53 are thought to contribute to a narrowed minor groove and a more negative electrostatic ...
... HG bps help avoid a steric clash between the guanine exocyclic NH2 group and a nearby leucine side chain (16). Two consecutive A•T HG bps in X-ray structures of DNA in complex with the tumor suppressor protein p53 are thought to contribute to a narrowed minor groove and a more negative electrostatic ...
Phylogenomics of type II DNA topoisomerases
... All these data suggest that Topo IIA and Topo IIB enzymes have been created independently by the assembly of several modules, some of them being common (the Bergerat fold, the CAP-like domain and the toprim domain), but other being unrelated. Thus, the Topo II activity has been most likely invented ...
... All these data suggest that Topo IIA and Topo IIB enzymes have been created independently by the assembly of several modules, some of them being common (the Bergerat fold, the CAP-like domain and the toprim domain), but other being unrelated. Thus, the Topo II activity has been most likely invented ...
An Introduction to Peptide Nucleic Acid
... DNA has many important characteristics that allow it to perform these functions. Two of the most important properties are the specificity and the reversible nature of the hydrogen bonding between complementary nucleobases, properties which allow the strands of the double helix to be unwound and then ...
... DNA has many important characteristics that allow it to perform these functions. Two of the most important properties are the specificity and the reversible nature of the hydrogen bonding between complementary nucleobases, properties which allow the strands of the double helix to be unwound and then ...
New peptide and gene coding for same
... al., Biochemistry 18, 5294-5299, 1979), and enriched for poly (A)+ RNA (mRNA) with an oligo (dT) cellulose column. The poly (A) RNA were used to prepare a cDNA library according to the Okayama-Berg method (Mol. Cell. Biol. 2, 161-170, 1982). The library was screened with a mixture of probes consisti ...
... al., Biochemistry 18, 5294-5299, 1979), and enriched for poly (A)+ RNA (mRNA) with an oligo (dT) cellulose column. The poly (A) RNA were used to prepare a cDNA library according to the Okayama-Berg method (Mol. Cell. Biol. 2, 161-170, 1982). The library was screened with a mixture of probes consisti ...
microencapsula tion of dna within cross
... homogenized in the chitosan solution for approximately 20 minutes to obtain a uniform suspension. 5% (w/v) carbonyl iron powder (GAF) was then added to the suspension. The organic phase consisted of 50 .ml sunflower oil (Sun Oueen) with 2% (v/v) Span 85 (Atkemix, Orantford, Ont.) as the emulsifier. ...
... homogenized in the chitosan solution for approximately 20 minutes to obtain a uniform suspension. 5% (w/v) carbonyl iron powder (GAF) was then added to the suspension. The organic phase consisted of 50 .ml sunflower oil (Sun Oueen) with 2% (v/v) Span 85 (Atkemix, Orantford, Ont.) as the emulsifier. ...
Quantitative Analysis of the Kinetics of End
... disassembly then proceeds from the newly created end (this assumption is addressed below). Note that although disassembly is a ®rst-order process, it will not follow classic ®rst-order kinetics. This is because the concentration of reactant, the ®lament ends, does not change as the reaction progress ...
... disassembly then proceeds from the newly created end (this assumption is addressed below). Note that although disassembly is a ®rst-order process, it will not follow classic ®rst-order kinetics. This is because the concentration of reactant, the ®lament ends, does not change as the reaction progress ...
quantitation of male and female dna in mixed biological samples
... isolated, polymorphic STR markers within the genome can be detected and analyzed following polymerase chain reaction (PCR) amplification (Butler, 2005). Multiplex PCRs are designed to amplify several loci simultaneously, producing alleles from multiple STR loci used for identification in a sample PC ...
... isolated, polymorphic STR markers within the genome can be detected and analyzed following polymerase chain reaction (PCR) amplification (Butler, 2005). Multiplex PCRs are designed to amplify several loci simultaneously, producing alleles from multiple STR loci used for identification in a sample PC ...
Switching between polymerase and exonuclease sites in DNA
... exonuclease active sites (16). B-family polymerases also contain a conserved KKRY motif that has been shown to be important for stabilizing the primer terminus in the polymerase active site (17). K967 in Pol ε is part of this KKRY motif and is homologous to K814 in Pol ␦, K706 in RB69 gp43 and K498 ...
... exonuclease active sites (16). B-family polymerases also contain a conserved KKRY motif that has been shown to be important for stabilizing the primer terminus in the polymerase active site (17). K967 in Pol ε is part of this KKRY motif and is homologous to K814 in Pol ␦, K706 in RB69 gp43 and K498 ...
AT3 (Acyltransferase) Gene Isolated from Capsicum frutescens cv
... Several enzymes are involved in Capsaicin biosynthesis. Capsaicin synthase (CS) is the last enzyme which has an important role in capsaicin biosynthesis by condensing vanillylamin with acyl moieties to produce capsaicin. CS also serves as a regulator for the formation of capsaicin [9]. Acyltransfera ...
... Several enzymes are involved in Capsaicin biosynthesis. Capsaicin synthase (CS) is the last enzyme which has an important role in capsaicin biosynthesis by condensing vanillylamin with acyl moieties to produce capsaicin. CS also serves as a regulator for the formation of capsaicin [9]. Acyltransfera ...
DNA-mediated transformation of the filamentous fungusAspergillus
... having each chromosome marked with a mutant gene) allows assignment of this mutation to a particular chromosome (Pontecorvo 1956). Parasexual analysis also allows the detection of chromosomal translocations. A translocation in one of the component strains of a diploid will cause linked segregation o ...
... having each chromosome marked with a mutant gene) allows assignment of this mutation to a particular chromosome (Pontecorvo 1956). Parasexual analysis also allows the detection of chromosomal translocations. A translocation in one of the component strains of a diploid will cause linked segregation o ...
Document
... Before a cell divides, the DNA strands unwind and separate Each strand makes a new partner by adding the appropriate nucleotides ...
... Before a cell divides, the DNA strands unwind and separate Each strand makes a new partner by adding the appropriate nucleotides ...
Mutagenesis Point mutations Deletions Insertions Types of
... vectors, which are then transformed into E. coli. One or more fragments have been routinely assembled with general cloning vectors, such as pUC19, and assembled into NEB’s pTYB1 expression vector (NEB #N6701). The latter approach was used to express several methylase genes, which aided the genome tr ...
... vectors, which are then transformed into E. coli. One or more fragments have been routinely assembled with general cloning vectors, such as pUC19, and assembled into NEB’s pTYB1 expression vector (NEB #N6701). The latter approach was used to express several methylase genes, which aided the genome tr ...
DNA PPT - Alevelsolutions
... Replication of DNA occurs (a) During cell division (b) before cell division ...
... Replication of DNA occurs (a) During cell division (b) before cell division ...
Studies on Polynucleotides
... dCTP were used. Experiment 3 in Table I shows the results obtained on nearest neighbor analysis of the product obtained when [cG2P]dCTP alone was used. As expected, the radi0activit.y Ivas in dAp and dCp, the ratio found being 1.3. The result may again indicate that the incorporation of the terminal ...
... dCTP were used. Experiment 3 in Table I shows the results obtained on nearest neighbor analysis of the product obtained when [cG2P]dCTP alone was used. As expected, the radi0activit.y Ivas in dAp and dCp, the ratio found being 1.3. The result may again indicate that the incorporation of the terminal ...
Chapter 22: SV40 and Polyomavirus DNA Replication
... viral DNA replication. The extent to which auxiliary elements enhance DNA replication differs between SV40 and PyV: SV40 DNA replication is stimulated 5- to 25-fold by these elements, whereas PyV DNA replication is enhanced between 100- and 1000-fold (Hassell et al. 1986). The ori-core of each virus ...
... viral DNA replication. The extent to which auxiliary elements enhance DNA replication differs between SV40 and PyV: SV40 DNA replication is stimulated 5- to 25-fold by these elements, whereas PyV DNA replication is enhanced between 100- and 1000-fold (Hassell et al. 1986). The ori-core of each virus ...