Electronic Fingerprints of DNA Bases on Graphene
... substrate compared to metals. We have calculated the tunneling conductance and LDOS for the specific case of single DNA bases at various orientations on the surface and have shown that the different nucleotides have significantly different LDOS peaks (fingerprints), allowing differentiation via loca ...
... substrate compared to metals. We have calculated the tunneling conductance and LDOS for the specific case of single DNA bases at various orientations on the surface and have shown that the different nucleotides have significantly different LDOS peaks (fingerprints), allowing differentiation via loca ...
Unraveling DNA helicases
... amino acids are shown by single-letter code and the letters in bold indicate highly conserved motifs. The accession numbers of aligned sequences of DNA helicases are: Pea (PDH45, Y17186), Rad3 (K03293), Rad15 (X6499), ERCC2 (X52470), ERCC3 (M31899) and RECQL (L36140). The accession numbers of aligne ...
... amino acids are shown by single-letter code and the letters in bold indicate highly conserved motifs. The accession numbers of aligned sequences of DNA helicases are: Pea (PDH45, Y17186), Rad3 (K03293), Rad15 (X6499), ERCC2 (X52470), ERCC3 (M31899) and RECQL (L36140). The accession numbers of aligne ...
An attempt to unify the structure of polymerases
... With the great availability of sequences from RNA- and DNAof DNA-dependent DNA polymerase, hereafter called the pol dependent RNA and DNA polymerases, it has become possible /3 type, has only two members: DNA polymerase /3 (Matsukage to delineate a few highly conserved regions for various etal., 198 ...
... With the great availability of sequences from RNA- and DNAof DNA-dependent DNA polymerase, hereafter called the pol dependent RNA and DNA polymerases, it has become possible /3 type, has only two members: DNA polymerase /3 (Matsukage to delineate a few highly conserved regions for various etal., 198 ...
3D DNA Crystals and Nanotechnology
... This simplicity is also reflected in the immediately recognizable relationship between the double helical structure and DNA’s role in genetic information storage. The original determination of the double helical DNA structure was itself deeply rooted in the exploration of another class of structures ...
... This simplicity is also reflected in the immediately recognizable relationship between the double helical structure and DNA’s role in genetic information storage. The original determination of the double helical DNA structure was itself deeply rooted in the exploration of another class of structures ...
DNA Science A Hands-On Workshop - nslc.wustl.edu
... In this lab exercise, PCR will be used to amplify a nucleotide sequence from human chromosome 8 to look for an insertion of a short DNA sequence called Alu, within the tissue plasminogen activator (TPA) gene. Alu sequences are thought to be derived from the 7SL RNA gene. This gene encodes for the RN ...
... In this lab exercise, PCR will be used to amplify a nucleotide sequence from human chromosome 8 to look for an insertion of a short DNA sequence called Alu, within the tissue plasminogen activator (TPA) gene. Alu sequences are thought to be derived from the 7SL RNA gene. This gene encodes for the RN ...
Replication of Nuclear DNA
... All eukaryotic organisms organise their DNA for replication as multiple units known as replicons. In cells undergoing DNA replication, these multiple units are readily visualised by fibre autoradiography (Cairns 1966; Huberman and Riggs 1968). This was first applied to plants by Van’t Hof (1975) and ...
... All eukaryotic organisms organise their DNA for replication as multiple units known as replicons. In cells undergoing DNA replication, these multiple units are readily visualised by fibre autoradiography (Cairns 1966; Huberman and Riggs 1968). This was first applied to plants by Van’t Hof (1975) and ...
Inactivation of the p53 Tumor Suppressor Gene
... well-studied example is that of gene inactivation by germ-line rear rangement in the low-density lipoprotein receptor gene (7). Two clusters of highly homologous Alu elements make this gene particu larly prone to gene rearrangements. Alu-associated recombination occurring in the germ line has also b ...
... well-studied example is that of gene inactivation by germ-line rear rangement in the low-density lipoprotein receptor gene (7). Two clusters of highly homologous Alu elements make this gene particu larly prone to gene rearrangements. Alu-associated recombination occurring in the germ line has also b ...
Crystal Structure of the Archaeal Heat Shock Regulator from
... High stability of the dimeric structure can be expected as more than one third of the amino acids from each subunit are involved in forming a large dimeric interface area (ca 4200 Å2). In addition to hydrophobic interactions, 18 hydrogen bonds as well as two salt-bridges are also formed at the inter ...
... High stability of the dimeric structure can be expected as more than one third of the amino acids from each subunit are involved in forming a large dimeric interface area (ca 4200 Å2). In addition to hydrophobic interactions, 18 hydrogen bonds as well as two salt-bridges are also formed at the inter ...
Protein–Nucleic Acid Interactions
... segment follows the surface curvature of the packaging protein, and here the bending of the DNA is associated with periodic changes in the geometry of the base steps. Finally, it should be noted that a DNA-binding protein seldom amounts to a rigid surface upon which the DNA moulds its shape: in the ...
... segment follows the surface curvature of the packaging protein, and here the bending of the DNA is associated with periodic changes in the geometry of the base steps. Finally, it should be noted that a DNA-binding protein seldom amounts to a rigid surface upon which the DNA moulds its shape: in the ...
Motifs that discriminate functions in genomic regions
... nonrepetitive DNA sequences (ChIP-chip, (4)) along a large segment (66 Mb) of mouse chromosome 7. Randomly sampled ChIP-chip positive regions were re-tested in a quantitative PCR assay using independent ChIP material, yielding very high validation rates for GATA1 occupancy. This dataset also provide ...
... nonrepetitive DNA sequences (ChIP-chip, (4)) along a large segment (66 Mb) of mouse chromosome 7. Randomly sampled ChIP-chip positive regions were re-tested in a quantitative PCR assay using independent ChIP material, yielding very high validation rates for GATA1 occupancy. This dataset also provide ...
Identifying the Genetic Material
... When Griffith injected mice with heat-killed S bacteria, the mice still lived. Thus, Griffith knew it was not the capsule on the S bacteria that killed the mice. He then mixed the harmless live R bacteria with the harmless heat-killed S bacteria. Mice injected with this mixture of previously harmles ...
... When Griffith injected mice with heat-killed S bacteria, the mice still lived. Thus, Griffith knew it was not the capsule on the S bacteria that killed the mice. He then mixed the harmless live R bacteria with the harmless heat-killed S bacteria. Mice injected with this mixture of previously harmles ...
MOLECULAR STRUCTURE OF DNA AND RNA
... viral genetic material, the bacterial cytoplasm provides all of the machinery necessary to make viral proteins and DNA. The viral proteins and DNA assemble to make new viruses that are subsequently released from the cell by lysis (i.e., cell breakage). To verify that DNA is the genetic material of T ...
... viral genetic material, the bacterial cytoplasm provides all of the machinery necessary to make viral proteins and DNA. The viral proteins and DNA assemble to make new viruses that are subsequently released from the cell by lysis (i.e., cell breakage). To verify that DNA is the genetic material of T ...
Thermodynamic analysis of DNA binding by a Bacillus single
... Results: We have cloned and purified SSB from Bacillus anthracis (SSBBA). In the absence of DNA, at concentrations ≤100 μg/ml, SSBBA did not form a stable tetramer and appeared to resemble bacteriophage T4 gene 32 protein. Fluorescence anisotropy studies demonstrated that SSBBA bound ssDNA with high ...
... Results: We have cloned and purified SSB from Bacillus anthracis (SSBBA). In the absence of DNA, at concentrations ≤100 μg/ml, SSBBA did not form a stable tetramer and appeared to resemble bacteriophage T4 gene 32 protein. Fluorescence anisotropy studies demonstrated that SSBBA bound ssDNA with high ...
Non-destructive DNA extraction methods that yield DNA barcodes in
... Ragupathy, 2013). This gene is ideal to barcode because it is found in all animal species and has regions of conserved sequence that allow amplification with common primers (Hebert et al., 2003; Barrett & Hebert, 2005; Hebert & Gregory, 2005; Mitchell, 2008). It was already known that DNA could be e ...
... Ragupathy, 2013). This gene is ideal to barcode because it is found in all animal species and has regions of conserved sequence that allow amplification with common primers (Hebert et al., 2003; Barrett & Hebert, 2005; Hebert & Gregory, 2005; Mitchell, 2008). It was already known that DNA could be e ...
Identification and removal of colanic acid from plasmid DNA
... obtained by HPLC analysis. Furthermore, the high MW CA and other extremely large polysaccharides most likely cannot load onto the HPLC columns used for assessing plasmid DNA preparations. Some investigators have attributed the observed plasmid toxicities solely to the presence of CpG sequences withi ...
... obtained by HPLC analysis. Furthermore, the high MW CA and other extremely large polysaccharides most likely cannot load onto the HPLC columns used for assessing plasmid DNA preparations. Some investigators have attributed the observed plasmid toxicities solely to the presence of CpG sequences withi ...
Education®
... weak bonding force found only between hydrogen and either nitrogen or oxygen atoms. 19. Introns – sections of the mRNA template that are cut out prior to translation into protein and, hence, do not code for amino acids. 20. Lagging Strand – the strand of double-stranded DNA that replicate ...
... weak bonding force found only between hydrogen and either nitrogen or oxygen atoms. 19. Introns – sections of the mRNA template that are cut out prior to translation into protein and, hence, do not code for amino acids. 20. Lagging Strand – the strand of double-stranded DNA that replicate ...
78780 TG DNA Replication and Transcription
... 4. Codon – a sequence of three (3) consecutive nitrogen-containing bases on mRNA that code for an amino acid or a stop signal. Codons are the basic component of the genetic code. 5. Complementary Base Pairs – specific pairs of nitrogen-containing bases that always bond together when double stranded ...
... 4. Codon – a sequence of three (3) consecutive nitrogen-containing bases on mRNA that code for an amino acid or a stop signal. Codons are the basic component of the genetic code. 5. Complementary Base Pairs – specific pairs of nitrogen-containing bases that always bond together when double stranded ...
the pdf of this lesson!
... 4. Codon – a sequence of three (3) consecutive nitrogen-containing bases on mRNA that code for an amino acid or a stop signal. Codons are the basic component of the genetic code. 5. Complementary Base Pairs – specific pairs of nitrogen-containing bases that always bond together when double stranded ...
... 4. Codon – a sequence of three (3) consecutive nitrogen-containing bases on mRNA that code for an amino acid or a stop signal. Codons are the basic component of the genetic code. 5. Complementary Base Pairs – specific pairs of nitrogen-containing bases that always bond together when double stranded ...
Chapter 6: DNA Replication and Telomere Maintenance I
... F. Eukaryotic Linear Replication: Leading Strand Synthesis 1. The leading strand is the easiest strand to synthesize namely because it occurs continuously 2. The template for the leading strand is the strand that goes 5’3’ away from the replication fork 3. The leading strand is synthesized in the 5 ...
... F. Eukaryotic Linear Replication: Leading Strand Synthesis 1. The leading strand is the easiest strand to synthesize namely because it occurs continuously 2. The template for the leading strand is the strand that goes 5’3’ away from the replication fork 3. The leading strand is synthesized in the 5 ...
NUCLEOTIDES AND NUCLEIC ACIDS
... All the phosphodiester linkages have the same orientation along the chain (Fig. 8–7), giving each linear nucleic acid strand a specific polarity and distinct 5 and 3 ends. By definition, the 5 end lacks a nucleotide at the 5 position and the 3 end lacks a nucleotide at the 3 position. Other gr ...
... All the phosphodiester linkages have the same orientation along the chain (Fig. 8–7), giving each linear nucleic acid strand a specific polarity and distinct 5 and 3 ends. By definition, the 5 end lacks a nucleotide at the 5 position and the 3 end lacks a nucleotide at the 3 position. Other gr ...
Detection method - Gmoinfo
... modified carnation line 26407 (Unique identifier: IFD-26407-2). In this context, the European Union Reference Laboratory for GM Food and Feed (EU-RL GMFF) was asked to carry out a singlelaboratory validation of the performance of a polymerase chain reaction (PCR)-based method for detecting and ident ...
... modified carnation line 26407 (Unique identifier: IFD-26407-2). In this context, the European Union Reference Laboratory for GM Food and Feed (EU-RL GMFF) was asked to carry out a singlelaboratory validation of the performance of a polymerase chain reaction (PCR)-based method for detecting and ident ...
Unit VI – Structure and Function of DNA and RNA Unit VI: Structure
... The DNA is stretched out in the nucleus as chromatin before cell division so that it can be read. DNA is the blueprint for the synthesis of proteins, and proteins determine the traits of an organism. Proteins are made up of amino acids. Each amino acid is determined by a sequence of 3 base pairs of ...
... The DNA is stretched out in the nucleus as chromatin before cell division so that it can be read. DNA is the blueprint for the synthesis of proteins, and proteins determine the traits of an organism. Proteins are made up of amino acids. Each amino acid is determined by a sequence of 3 base pairs of ...
DNA damage and decisions: CtIP coordinates DNA repair and cell
... Mre11–Rad50–NBS1 (MRN) complex [9–11]. CtIP undergoes additional phosphorylation by the checkpoint protein kinase ATM in response to DSB damage [12]. Although the underlying mechanism remains controversial, ATM phosphorylation of CtIP leads to transcription of cell cycle inhibitor genes, such as p21 ...
... Mre11–Rad50–NBS1 (MRN) complex [9–11]. CtIP undergoes additional phosphorylation by the checkpoint protein kinase ATM in response to DSB damage [12]. Although the underlying mechanism remains controversial, ATM phosphorylation of CtIP leads to transcription of cell cycle inhibitor genes, such as p21 ...
Strategies for the in vitro Evolution of Protein
... Bacillus subtilis p-nitrobenzyl (pNB) esterase catalyzes the hydrolysis of the para-nitrobenzyl esters of various cephalosporin-type antibiotics, a necessary step in their large-scale synthesis (Zock et al., 1994). Using four generations of sequential random mutagenesis and screening, we evolved a s ...
... Bacillus subtilis p-nitrobenzyl (pNB) esterase catalyzes the hydrolysis of the para-nitrobenzyl esters of various cephalosporin-type antibiotics, a necessary step in their large-scale synthesis (Zock et al., 1994). Using four generations of sequential random mutagenesis and screening, we evolved a s ...
A Mutation Causing Reduced Biological Activity and Stability of
... within a 14 kbp EcoRi fragment and is composed of 4 protein coding exons (Fig. 2). The gene of the affected father (II-2, see Fig. 1) of the proband was isolated, and the sequences of these 4 exons were compared to those of a normal human liver TBG cDNA clone and a genomic TBG clone from a human X c ...
... within a 14 kbp EcoRi fragment and is composed of 4 protein coding exons (Fig. 2). The gene of the affected father (II-2, see Fig. 1) of the proband was isolated, and the sequences of these 4 exons were compared to those of a normal human liver TBG cDNA clone and a genomic TBG clone from a human X c ...