Principle and applications of digital PCR
... tumor. As the mucinous adenoma is a single layer of neoplastic cells embedded in abundant stromal cells and mucin, traditional methods, such as direct nucleotide sequencing, may not be sensitive enough to detect KRAS mutations, even when laser capture microdissection is employed to enrich the tumor ...
... tumor. As the mucinous adenoma is a single layer of neoplastic cells embedded in abundant stromal cells and mucin, traditional methods, such as direct nucleotide sequencing, may not be sensitive enough to detect KRAS mutations, even when laser capture microdissection is employed to enrich the tumor ...
OsHUS1 Facilitates Accurate Meiotic Recombination in Rice
... recombination (ER, also known as non-allelic homologous recombination). As eukaryotic genomes are rich in repeated DNA sequences, ER can produce chromosomal rearrangements, which in humans result in numerous genomic disorders [9,10]. Despite the adverse impact of ER on genome integrity, ER occurs re ...
... recombination (ER, also known as non-allelic homologous recombination). As eukaryotic genomes are rich in repeated DNA sequences, ER can produce chromosomal rearrangements, which in humans result in numerous genomic disorders [9,10]. Despite the adverse impact of ER on genome integrity, ER occurs re ...
DNA crosslinking and biological activity of a
... structure and high reactivity, nitrogen mustards have a very limited DNA sequence selectivity and rapidly lose their activity by non-speci®c interaction with a number of nucleophilic groups on cellular biomolecules other than DNA. Nitrogen mustards have been shown to be potentially carcinogenic, mut ...
... structure and high reactivity, nitrogen mustards have a very limited DNA sequence selectivity and rapidly lose their activity by non-speci®c interaction with a number of nucleophilic groups on cellular biomolecules other than DNA. Nitrogen mustards have been shown to be potentially carcinogenic, mut ...
Conformation of DNA in chromatin protein
... in the stabilization of the B conformation we have studied chromatin depleted from different proteins by 0.6 M NaCl and reconstituted NHP-DNA and histone-DNA complexes. The electrophoretic patterns of the proteins extracted by urea-NaCl treatment of chromatin and of chromatin previously treated with ...
... in the stabilization of the B conformation we have studied chromatin depleted from different proteins by 0.6 M NaCl and reconstituted NHP-DNA and histone-DNA complexes. The electrophoretic patterns of the proteins extracted by urea-NaCl treatment of chromatin and of chromatin previously treated with ...
Physical and Topological Properties of Circular DNA
... JEROME VINOGRAD and JACOB LEBOWITZ From the Divisions of Biology and Chemistry, California Institute of Technology, Pasadena. Dr. Lebowitz' present address is the Department of Bacteriology and Botany, Syracuse University ...
... JEROME VINOGRAD and JACOB LEBOWITZ From the Divisions of Biology and Chemistry, California Institute of Technology, Pasadena. Dr. Lebowitz' present address is the Department of Bacteriology and Botany, Syracuse University ...
Honor Genetics DNA structure and replication
... 5 direction by addition of short segments, Okazaki fragments, that grow ...
... 5 direction by addition of short segments, Okazaki fragments, that grow ...
Spectrum-Like Graphical Representation of DNA Based on Codons
... underlying RNA codons are known, as already outlined in ref. [18]. Moreover, even when codons determining a particular protein are not known (which is going to be often the case) one can arrive at 1-D spectrum-like representations of proteins using the Virtual Genetic Code,25 which assigns to each a ...
... underlying RNA codons are known, as already outlined in ref. [18]. Moreover, even when codons determining a particular protein are not known (which is going to be often the case) one can arrive at 1-D spectrum-like representations of proteins using the Virtual Genetic Code,25 which assigns to each a ...
Document
... solution. Sequence effects on DNA cleavage patterns obtained by PAGE were studied using consequent mono, di and tetranucleotide levels of description. Our analysis revealed the enhancement of cleavage rate in phosphodiester bond following deoxycitidine. At the dinucleotide level, cleavage rate of th ...
... solution. Sequence effects on DNA cleavage patterns obtained by PAGE were studied using consequent mono, di and tetranucleotide levels of description. Our analysis revealed the enhancement of cleavage rate in phosphodiester bond following deoxycitidine. At the dinucleotide level, cleavage rate of th ...
Preventing transcriptional gene silencing by active DNA demethylation
... In contrast to the large amount of information that has accumulated on DNA methylation, relatively little is known about DNA demethylation. Current understanding of the mechanisms responsible for the maintenance of DNA methylation patterns in somatic tissues are based on the supposition that DNA met ...
... In contrast to the large amount of information that has accumulated on DNA methylation, relatively little is known about DNA demethylation. Current understanding of the mechanisms responsible for the maintenance of DNA methylation patterns in somatic tissues are based on the supposition that DNA met ...
Cell-cycle-specific activators of the Mec1/ATR
... In vitro activation of Mec1 by Dpb11/TopBP1 S. cerevisiae Dpb11, the orthologue of vertebrate TopBP1 and S. pombe Cut5, is an essential replication protein with several BRCT (BRCA1 C-terminal domain) motifs. Binding of phosphorylated replication initiation factors to these BRCT domains defines the C ...
... In vitro activation of Mec1 by Dpb11/TopBP1 S. cerevisiae Dpb11, the orthologue of vertebrate TopBP1 and S. pombe Cut5, is an essential replication protein with several BRCT (BRCA1 C-terminal domain) motifs. Binding of phosphorylated replication initiation factors to these BRCT domains defines the C ...
Finding Data in DNA: Computer Forensic Investigations of Living
... lengths of DNA sequences that may or may not be three bases in length. While not codons in the strict biological sense, we will refer to these encoding patterns as codons for the purpose of this manuscript. 1.1 DNA Computing DNA computing is an emerging new research field that uses DNA molecules ins ...
... lengths of DNA sequences that may or may not be three bases in length. While not codons in the strict biological sense, we will refer to these encoding patterns as codons for the purpose of this manuscript. 1.1 DNA Computing DNA computing is an emerging new research field that uses DNA molecules ins ...
A systematic search for DNA methyltransferase polymorphisms
... methylation patterns during gametogenesis and early embryogenesis (15). The DNMT1 enzyme acts primarily as a maintenance DNMT ensuring the faithful replication of DNA methylation patterns at each cell division (16,17). In addition to these active enzymes, the DNMT3L protein, while catalytically inac ...
... methylation patterns during gametogenesis and early embryogenesis (15). The DNMT1 enzyme acts primarily as a maintenance DNMT ensuring the faithful replication of DNA methylation patterns at each cell division (16,17). In addition to these active enzymes, the DNMT3L protein, while catalytically inac ...
13 Interplay Between H2AX and 53BP1 Pathways in DNA Double
... during mitosis can result in deletions of a large proportion of the genetic information passed to the progeny cells. If not properly repaired, DSBs can cause alterations in the DNA sequence, chromosomal translocations, genomic instability, and eventually neoplastic transformation. Thus, it is not su ...
... during mitosis can result in deletions of a large proportion of the genetic information passed to the progeny cells. If not properly repaired, DSBs can cause alterations in the DNA sequence, chromosomal translocations, genomic instability, and eventually neoplastic transformation. Thus, it is not su ...
A Human Centromere Protein, CENP-B, Has a DNA Binding Domain
... rein, designated CENP-D, has been identified also using the autoimmune sera (Kingwell and Rattner, 1987). Recently, it has been suggested that a motor protein, cytoplasmic dynein, takes part in centromere function (Pfarr et al., 1990; Steuer et al., 1990). Several monoclonal antibodies that recogniz ...
... rein, designated CENP-D, has been identified also using the autoimmune sera (Kingwell and Rattner, 1987). Recently, it has been suggested that a motor protein, cytoplasmic dynein, takes part in centromere function (Pfarr et al., 1990; Steuer et al., 1990). Several monoclonal antibodies that recogniz ...
Four-color DNA sequencing by synthesis using cleavable
... resulting nucleotide analogues should be able to incorporate into the growing DNA strand as terminators. Based on this rationale, we proposed an SBS approach using cleavable fluorescent nucleotide analogues as reversible terminators to sequence surfaceimmobilized DNA [supporting information (SI) Fig ...
... resulting nucleotide analogues should be able to incorporate into the growing DNA strand as terminators. Based on this rationale, we proposed an SBS approach using cleavable fluorescent nucleotide analogues as reversible terminators to sequence surfaceimmobilized DNA [supporting information (SI) Fig ...
Protein–DNA Hydrophobic Recognition in the Minor Groove
... remained in “standard” B configuration. In other words, two schemes are being used to facilitate the recognition: in addition to “digital” H-bond recognition, the “analogue” one helps in fitting to structural constraints in the complexes.12 So, there is no simple code for protein –DNA recognition.13 ...
... remained in “standard” B configuration. In other words, two schemes are being used to facilitate the recognition: in addition to “digital” H-bond recognition, the “analogue” one helps in fitting to structural constraints in the complexes.12 So, there is no simple code for protein –DNA recognition.13 ...
Programmed Materials Synthesis with DNA
... the oligonucleotide strands that comprise them.57,58 The two classes of molecules are referred to as “parallel” (DP) and “antiparallel” (DA). In this review, the focus will be solely on the DA class of DX molecules because of their superior mechanical properties.58 The two DA molecules discussed her ...
... the oligonucleotide strands that comprise them.57,58 The two classes of molecules are referred to as “parallel” (DP) and “antiparallel” (DA). In this review, the focus will be solely on the DA class of DX molecules because of their superior mechanical properties.58 The two DA molecules discussed her ...
Chapter 19: DNA Ligases - DNA Replication and Human
... (Hsieh et al. 1993). Since the 46BR patient was sun sensitive, and the late steps of lagging-strand DNA replication and nucleotide excision repair share many factors (Aboussekhra et al. 1995), it seems likely that DNA ligase I is responsible for the final DNA-joining step in nucleotide excision repa ...
... (Hsieh et al. 1993). Since the 46BR patient was sun sensitive, and the late steps of lagging-strand DNA replication and nucleotide excision repair share many factors (Aboussekhra et al. 1995), it seems likely that DNA ligase I is responsible for the final DNA-joining step in nucleotide excision repa ...
D-loop - BioMed Central
... molecular weight of the two strands of the mitochondrial DNA, which are termed heavy and light. The G-rich heavy strand encodes the majority of genes. Its transcript is, thus, G-poor and C-rich (negative GC-skew and positive AT-skew). During replication, the light strand is the leading strand, and i ...
... molecular weight of the two strands of the mitochondrial DNA, which are termed heavy and light. The G-rich heavy strand encodes the majority of genes. Its transcript is, thus, G-poor and C-rich (negative GC-skew and positive AT-skew). During replication, the light strand is the leading strand, and i ...
Leishmania donovani - Oxford Academic
... positioned above the DNA major groove, but do not directly interact with the DNA. The second lobe (also comprising two domains; the former resembling the core subdomain III of human topoisomerase I and the latter representing a short variable C-terminal domain) sits below the DNA, is composed of an ...
... positioned above the DNA major groove, but do not directly interact with the DNA. The second lobe (also comprising two domains; the former resembling the core subdomain III of human topoisomerase I and the latter representing a short variable C-terminal domain) sits below the DNA, is composed of an ...
An Introduction to DNA and Genetic Genealogy
... Inheritance of Y-DNA and mtDNA Understanding DNA inheritance is useful when determining which person to test to support or disprove any given hypothesis. The following chart illustrates the inheritance of Y-DNA and mtDNA from an ancestral couple. Males are indicated by a square, females by a circle ...
... Inheritance of Y-DNA and mtDNA Understanding DNA inheritance is useful when determining which person to test to support or disprove any given hypothesis. The following chart illustrates the inheritance of Y-DNA and mtDNA from an ancestral couple. Males are indicated by a square, females by a circle ...
Isolation of the b-tubulin Gene From Yeast and Demonstration of its Essential Function in vivo.
... al., 1980). A similar result has recently been obtained with S. cerevisiae strains resistant to the benomyl derivative MBC (P. Baum and J. Thorner, unpublished data). The Aspergillus results suggested to us that if benomyl-resistant yeast strains are /3-tubulin mutants as well, the resistance phenot ...
... al., 1980). A similar result has recently been obtained with S. cerevisiae strains resistant to the benomyl derivative MBC (P. Baum and J. Thorner, unpublished data). The Aspergillus results suggested to us that if benomyl-resistant yeast strains are /3-tubulin mutants as well, the resistance phenot ...
File
... Avery and other scientists discovered that a. DNA is found in a protein coat. b. DNA stores and transmits genetic information from one generation to the next. c. transformation does not affect bacteria. d. proteins transmit genetic information from one generation to the next. ...
... Avery and other scientists discovered that a. DNA is found in a protein coat. b. DNA stores and transmits genetic information from one generation to the next. c. transformation does not affect bacteria. d. proteins transmit genetic information from one generation to the next. ...
Original Article Accurate quantification standards of DNA via
... transferred. Multiple combustion experiments at 10 bar of O2 pressure were carried out using varying amounts of HCl (100-250 µL, 7.5%) and hydrogen peroxide (0.5-3mL, 30% v/v) in the absorbing solution and the mineralization efficiency was quantitated by measuring residual carbon content (RCC) [26] ...
... transferred. Multiple combustion experiments at 10 bar of O2 pressure were carried out using varying amounts of HCl (100-250 µL, 7.5%) and hydrogen peroxide (0.5-3mL, 30% v/v) in the absorbing solution and the mineralization efficiency was quantitated by measuring residual carbon content (RCC) [26] ...