Mycobacterium tuberculosis DNA gyrase ATPase domain structures
... [7,8] and ATPase activity [9,10]. The structure of the ATPase domain of the GyrB subunit of Escherichia coli DNA gyrase [7] showed that it contained two structural domains, an N-terminal GHKL domain [11] and a Cterminal transducer domain (Figure 1). A change in the relative positions of the GHKL and ...
... [7,8] and ATPase activity [9,10]. The structure of the ATPase domain of the GyrB subunit of Escherichia coli DNA gyrase [7] showed that it contained two structural domains, an N-terminal GHKL domain [11] and a Cterminal transducer domain (Figure 1). A change in the relative positions of the GHKL and ...
structure and mechanism of dna polymerases
... active role, rather than providing a platform for zipping DNA. A second model proposed that DNA polymerases select correct over incorrect nucleotides due to base pair geometry (Bruskov and Poltev, 1979; Engel and von Hippel, 1978; Sloane et al., 1988). In this case, the shape of the DNA polymerase a ...
... active role, rather than providing a platform for zipping DNA. A second model proposed that DNA polymerases select correct over incorrect nucleotides due to base pair geometry (Bruskov and Poltev, 1979; Engel and von Hippel, 1978; Sloane et al., 1988). In this case, the shape of the DNA polymerase a ...
The Role of DNA-PKcs and Artemis in Opening Viral DNA
... 3,000 ds-vg/dge). Unusual bands that were not present in samples from wild-type mice were observed in PstI (noncutter) DNA digests in DNA-PKcs-deficient mouse livers (shown with arrows). The major unusual bands migrated slightly faster than a 3,049-bp marker, which is 20 bp shorter than full-length ...
... 3,000 ds-vg/dge). Unusual bands that were not present in samples from wild-type mice were observed in PstI (noncutter) DNA digests in DNA-PKcs-deficient mouse livers (shown with arrows). The major unusual bands migrated slightly faster than a 3,049-bp marker, which is 20 bp shorter than full-length ...
Crystal Structure of Paired Domain -...
... embryo. All Pax genes (except Pax-1 and Pax-9 which are expressed in the developing vertebral column) are expressed in the developing neural tube and brain, and contribute to early nervous system development (Chalepakis et al.,1993; Noll, 1993; Stoykova and Gruss, 1994). Unlike Hox genes, which are ...
... embryo. All Pax genes (except Pax-1 and Pax-9 which are expressed in the developing vertebral column) are expressed in the developing neural tube and brain, and contribute to early nervous system development (Chalepakis et al.,1993; Noll, 1993; Stoykova and Gruss, 1994). Unlike Hox genes, which are ...
Immobilization_Mecha..
... available for hybridization to target DNA. To determine if these differences were based on the degree of probe immobilization or probe availability for hybridization, fluorescently labeled DNA probes were patterned onto the surfaces. In addition, double stranded DNA (dsDNA) solutions were prepared by ...
... available for hybridization to target DNA. To determine if these differences were based on the degree of probe immobilization or probe availability for hybridization, fluorescently labeled DNA probes were patterned onto the surfaces. In addition, double stranded DNA (dsDNA) solutions were prepared by ...
Synthesis and Evaluation of Guanidino Phthalocyanines for G
... participates in the structure formation, it is stabilized by negative supercoiling. Within HDNA, the triplex is stabilized by either Hoogsteen (YRY triplex) or reverse Hoogsteen (RRY triplex) interactions, depending upon whether the pyrimidine or the purine-rich strand participates in the triplex fo ...
... participates in the structure formation, it is stabilized by negative supercoiling. Within HDNA, the triplex is stabilized by either Hoogsteen (YRY triplex) or reverse Hoogsteen (RRY triplex) interactions, depending upon whether the pyrimidine or the purine-rich strand participates in the triplex fo ...
The Art of DNA Strings: Sixteen Years of DNA Coding Theory
... Template Based Method: This method was introduced in [58]–[60] that involves two step process. Initially a template is designed which is mapped to binary error correcting codes and combination of template and codeword results into DNA codewords. This method is not optimal because the code size is li ...
... Template Based Method: This method was introduced in [58]–[60] that involves two step process. Initially a template is designed which is mapped to binary error correcting codes and combination of template and codeword results into DNA codewords. This method is not optimal because the code size is li ...
Solving a Crime Using DNA Analysis and Chemistry
... The manager of the college food services promised the president of the college a plate of his favorite chocolate chip cookies for the upcoming meeting with the executive council of the Board of Directors. The chocolate chip cookies are a specialty of Chef Lombardo's and are famous across the college ...
... The manager of the college food services promised the president of the college a plate of his favorite chocolate chip cookies for the upcoming meeting with the executive council of the Board of Directors. The chocolate chip cookies are a specialty of Chef Lombardo's and are famous across the college ...
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... scientists at the Rockefeller Institute in New York decided to repeat Griffith’s work. Led by the Canadian biologist Oswald Avery, the scientists wanted to determine which molecule in the heat-killed bacteria was most important for transformation. They reasoned that if they could find this particular ...
... scientists at the Rockefeller Institute in New York decided to repeat Griffith’s work. Led by the Canadian biologist Oswald Avery, the scientists wanted to determine which molecule in the heat-killed bacteria was most important for transformation. They reasoned that if they could find this particular ...
Poly ADP-ribosylation: a histone shuttle mechanism in DNA excision
... In DNA excision repair of mammalian cells, the tight association of histones with DNA is locally disrupted. Newly synthesized repair patches appear transiently in DNA regions which are accessible to chemical and enzymatic probes. In this process, nucleosomes are unfolded concomitant with incision (S ...
... In DNA excision repair of mammalian cells, the tight association of histones with DNA is locally disrupted. Newly synthesized repair patches appear transiently in DNA regions which are accessible to chemical and enzymatic probes. In this process, nucleosomes are unfolded concomitant with incision (S ...
Use of novel assays to measure in vivo base excision DNA repair
... Cellular DNA constantly faces assault from various endogenous and exogenous agents. Nucleotides are oxidized on exposure to reactive oxygen species (ROS) which are generated endogenously during various cellular metabolic processes (Friedberg et al., 2006a, chap. 2). Oxidized guanine products such as ...
... Cellular DNA constantly faces assault from various endogenous and exogenous agents. Nucleotides are oxidized on exposure to reactive oxygen species (ROS) which are generated endogenously during various cellular metabolic processes (Friedberg et al., 2006a, chap. 2). Oxidized guanine products such as ...
A structural determinant in the uracil DNA glycosylase superfamily
... was cloned into pET21a as described above. The recombinant plasmid (pET21a-UNG-N123A) containing the desired mutated gene was confirmed by DNA sequencing and electroporated into E. coli strain BH214 (mug− , ung− ). The E. coli MUG and UNG mutant proteins were expressed and purified as described abov ...
... was cloned into pET21a as described above. The recombinant plasmid (pET21a-UNG-N123A) containing the desired mutated gene was confirmed by DNA sequencing and electroporated into E. coli strain BH214 (mug− , ung− ). The E. coli MUG and UNG mutant proteins were expressed and purified as described abov ...
CORPORATE RESPONSIBILITY AT DNA CORPORATE
... in Lahti for four years now. I had not given that much thought to responsibility from the company point of view, or how wide-spread its scope is, until I participated in the training last spring. While I may not be the most ecoconscious person out there, it was great to hear about DNA’s commitment a ...
... in Lahti for four years now. I had not given that much thought to responsibility from the company point of view, or how wide-spread its scope is, until I participated in the training last spring. While I may not be the most ecoconscious person out there, it was great to hear about DNA’s commitment a ...
Mutual Interactions of the Phosphate Groups in Locally Deformed
... solutions. The theory is now available (Soumpasis 1984). In spite of an approximative nature, it has gained credibility in recent years by being capable not only to reproduce the B-to-Z isomerization in DNA (Soumpasis et al. 1987b; ...
... solutions. The theory is now available (Soumpasis 1984). In spite of an approximative nature, it has gained credibility in recent years by being capable not only to reproduce the B-to-Z isomerization in DNA (Soumpasis et al. 1987b; ...
Structural basis of PAM-dependent target DNA recognition by the
... domains1,2,7. Cas9 has been exploited in numerous gene-targeting applications, in which its sequence specificity is programmed by either dual crRNA–tracrRNA guides or chimaeric single-molecule guide RNAs (sgRNAs)8–19. PAM recognition is a critical aspect of Cas9-mediated DNA targeting, being a prere ...
... domains1,2,7. Cas9 has been exploited in numerous gene-targeting applications, in which its sequence specificity is programmed by either dual crRNA–tracrRNA guides or chimaeric single-molecule guide RNAs (sgRNAs)8–19. PAM recognition is a critical aspect of Cas9-mediated DNA targeting, being a prere ...
The National DNA Database
... Act 1994, it has been overseen by a Management Board operated under a Memorandum of Understanding between the Forensic Science Service (FSS) and the Association of Chief Police Officers (ACPO). Recent developments, including the Home Office DNA Expansion Programme, the Review of the Forensic Science ...
... Act 1994, it has been overseen by a Management Board operated under a Memorandum of Understanding between the Forensic Science Service (FSS) and the Association of Chief Police Officers (ACPO). Recent developments, including the Home Office DNA Expansion Programme, the Review of the Forensic Science ...
voltammetric methods for determination of some anticancer drugs
... double helice and formed by altemating phosphate and pentose groups, in which the phosphodiester bridges provide the covalent continuity. The two chains of the double helix are held together by hydrogen bonds between purines and pyrimidine bases. The first DNA, structure proposed by Watson and Crick ...
... double helice and formed by altemating phosphate and pentose groups, in which the phosphodiester bridges provide the covalent continuity. The two chains of the double helix are held together by hydrogen bonds between purines and pyrimidine bases. The first DNA, structure proposed by Watson and Crick ...
Analysis of Guanine Oxidation Products in Double
... 2.2.2. Isolation and Identification of Oligomers Containing Iz, Gh or 8oxoG In Figure 7A, seven major peaks were detected at 16.6, 17.3, 18.7, 19.7, 20.9, 23.7, and 28.7 min. The oligomers containing guanine oxidation products, d(TXGGGT), d(TGXGGT), d(TGGXGT) or d(TGGGXT) (X = Iz, Gh or 8oxoG) were ...
... 2.2.2. Isolation and Identification of Oligomers Containing Iz, Gh or 8oxoG In Figure 7A, seven major peaks were detected at 16.6, 17.3, 18.7, 19.7, 20.9, 23.7, and 28.7 min. The oligomers containing guanine oxidation products, d(TXGGGT), d(TGXGGT), d(TGGXGT) or d(TGGGXT) (X = Iz, Gh or 8oxoG) were ...
Meiotic Recombination inSchizosaccharomyces pombe: A Paradigm
... Homologous genetic recombination plays two important roles during meiosis, the special nuclear divisions during which chromosome number is reduced from two (diploid) to one (haploid). First, recombination provides the physical connection between homologs that aids their pairing and proper segregatio ...
... Homologous genetic recombination plays two important roles during meiosis, the special nuclear divisions during which chromosome number is reduced from two (diploid) to one (haploid). First, recombination provides the physical connection between homologs that aids their pairing and proper segregatio ...
Have a break: determinants of meiotic DNA double strand break
... The yeast genome is small, and genes and their regulatory sequences are tightly packed, leaving hardly any space for heterochromatin (Zickler and Kleckner, 1999). The genomes of higher eukaryotes, in contrast, are often extremely large, and the coordination of DSB formation throughout the genome may ...
... The yeast genome is small, and genes and their regulatory sequences are tightly packed, leaving hardly any space for heterochromatin (Zickler and Kleckner, 1999). The genomes of higher eukaryotes, in contrast, are often extremely large, and the coordination of DSB formation throughout the genome may ...
dissertationes biologicae universitatis tartuensis 118
... domains — 1A, 1B, 2A and 2B (Fig. 1A). Domains 1A and 2A have the same fold, which is also very similar to the central region structure of the E. coli recombinase RecA (Story et al., 1992). Domains 1B and 2B are formed of the amino acid sequence extensions from 1A and 2A correspondingly. Their struc ...
... domains — 1A, 1B, 2A and 2B (Fig. 1A). Domains 1A and 2A have the same fold, which is also very similar to the central region structure of the E. coli recombinase RecA (Story et al., 1992). Domains 1B and 2B are formed of the amino acid sequence extensions from 1A and 2A correspondingly. Their struc ...
draft dna profiling bill 2007
... biological relationship, if any, between two individuals, living or dead without any doubt. With the objective to enhance protection of people in the society and administration of justice, analysis of DNA found at the scene of crime, of the victim or offender has been used to establish identity. The ...
... biological relationship, if any, between two individuals, living or dead without any doubt. With the objective to enhance protection of people in the society and administration of justice, analysis of DNA found at the scene of crime, of the victim or offender has been used to establish identity. The ...
Generalized Transduction by Phage P22 in Salmonella typhimurium. II. Mechanisms of Integration of Transducing DNA.
... in this experiment the density of the his+ particles can be calculated from the known densities of the phage (p = 1.506 g/cm3) and the pur+ transducing particles (p = l-511 g/om3). The average density of the his+ transducing particles in this gradient is calculated to be approximately l-512 g/cm3 (a ...
... in this experiment the density of the his+ particles can be calculated from the known densities of the phage (p = 1.506 g/cm3) and the pur+ transducing particles (p = l-511 g/om3). The average density of the his+ transducing particles in this gradient is calculated to be approximately l-512 g/cm3 (a ...
Impact of Sample Type and DNA Isolation Procedure on
... sequencing. Our results suggest that inferred community composition was dependent on ...
... sequencing. Our results suggest that inferred community composition was dependent on ...
13 The Klenow fragment of DNA polymerase I Pol I actually appears
... The active site residues lie in palm domain. The primer terminus is believed to interact with tyrosine 776 (Y776). In addition, two essential aspartate residues, D705 and D882, also lie at the base of the palm. As we shall see later, it is these acidic residues that bind the Mg 2+ ions that are esse ...
... The active site residues lie in palm domain. The primer terminus is believed to interact with tyrosine 776 (Y776). In addition, two essential aspartate residues, D705 and D882, also lie at the base of the palm. As we shall see later, it is these acidic residues that bind the Mg 2+ ions that are esse ...