Eram_SeyedMohammad - UWSpace
... I hereby declare that I am the sole author of this thesis. This is a true copy of the thesis, including any
required final revisions, as accepted by my examiners.
I understand that my thesis may be made electronically available to the public.
... this mechanism were proposed by Gacesa (1987). Glucan polysaccharides can also be degraded by phosphorolysis by such
enzymes as α-glucan phosphorylases (Withers et al. 1981).
Eukaryotic enzymes that depolymerize polysaccharides
predominantly utilize hydrolytic mechanisms while the lytic
mechanism is ...
Bioretrosynthetic Construction of a Non
... the past several years. In addition to Dr. Bachmann, I would also like to thank my
Dissertation Committee members of Dr. Richard Armstrong, Dr. Tina Iverson, Dr.
Lawrence Marnett and Dr. Carmello Rizzo for their valued discussions and for offering
their time and energy in assisting my training as a ...
Characterization of the regulatory function of the 46
... Arabidopsis Rubisco activase was recently shown to be regulated by redox changes in the larger (46-kDa) isoform
specifically mediated by thioredoxin-f [Zhang and Portis (1999) Proc Natl Acad Sci USA 96: 9438–9443]. Reduction greatly increases the activity of the 46-kDa isoform and the native protein ...
Branched-chain amino acid restriction in Zucker
... 12 h light:dark cycle with ad libitum access to water and food for up to
15 weeks. Food intake and weight gain were monitored weekly. A nonfasting blood sample was taken after 9 weeks on the diets at 9AM to
determine the genotype and dietary effect on circulating amino acid
concentrations. Plasma an ...
1 Chapter 1 Chemistry On The Pyrimidine Ring
... use a binuclear metal center (26). Lower metal content for dihydropyrimidase led early
researchers to also conclude that it was a mononuclear zinc enzyme. This phenomenon is
due to pH playing an important part in the metal binding to amidohydrolases. Both
dihydroorotases and dihydropyrimidases requi ...
E. Coli MurG: A Paradigm for a Superfamily of
... residues. The alignment shows that a relatively small
number of residues are invariant across the range of MurG
homologs (Fig. (5)). These residues are confined to five
different regions, three of which are the glycine-rich loops
that are proposed to be involved in binding the diphosphates
of the su ...
Characterization of the cDNA and Gene Coding for the Biotin
... Ward (personal communication) and to the partia1 sequence reported in GenBank accession number L34413. The
major differences between these three sequences are at the
5' and 3' noncoding ends. At the 5' end, the pBS-1 sequence
is 122 nucleotides longer than the sequence shown in GenBank accession num ...
... neutral pH’s are either basic or acidic
depending upon their AA composition.
Most proteins placed into basic conditions
become negatively charged. Acidic
conditions cause most proteins to develop
a positive charge.
... In the courseof these processesthe food substancesare transformed into compounds with high energy bonds (I) which can contribute to the pool of pyrophosphate nucleotides. In theselatter compoundsenergy is stored in a form immediately
available for the purpose of biosynthetic or mechanical work. Oxid ...
An Investigation of Acetobacter aceti N5
... indicate that the two do not differ significantly, indicating there has been no adaptive
change in enzyme mechanism. Also reported is a summary and analysis of a number of
crystal structures that have been determined for AaPurE, which suggests a strategy by
which proteins may have become resistant t ...
Purification and properties of NADP +-dependent
... batch prepared from between one to five residues per
mole. In the purified A . niger enzyme one ornithine
residue was found. The presence of ornithine was
confirmed independently by the methanesulphonic acid
and performic acid hydrolysis methods. The modification must be due to the action of an argi ...
Quantitative Analysis of the Kinetics of End
... structure. The ATP concentration is maintained at
high levels so that each DNA-bound RecA monomer is functioning at its kcat. The kcat employed is
that observed in the presence of longer random
sequence ssDNA molecules. Under conditions used
in this series of experiments, the kcat for ATP
ATP production in brain and liver mitochondria of Fischer
... temperature of the larger assay volume of the reaction mixture (990 l), which is kept at 28°C. Like the standards, the
background of the reaction mixtures (990 l) was recorded
before the addition of mitochondria. Afterward, 10 l of
freshly isolated mitochondria from the frontal cortex of the
Studies Into the Allosteric Regulation of ADP
... In glycogen, chains of α-1,4-polyglucans link with other chains to form a branching
pattern. In mammals and yeast, glycogen synthesis requires a 'seed' of glycogenin, which
is a self-glucosylating initiator protein, although no analogue of glycogen has been
described in bacteria.4 Glycogen is a larg ...
Interactions of TCA cycle enzymes and of the CcpA
... has already been characterized in other organisms. Moreover, the enzymatic reaction of Mdh, the endergonic
conversion of malate to oxaloacetate, is only driven by the fast conversion of oxaloacetate by CitZ. Since no
direct interaction between Mdh and CitZ was detected, other factors, e.g. other TCA ...
Purification and Characterization of Two Thermostable Proteases
... to homogeneity by fractional ammonium sulfate precipitation,
ion-exchange chromatography on DEAE-Sepharose, and PhenylSepharose hydrophobic interaction chromatography. By SDSPAGE, the molecular mass of the two purified enzymes was
estimated to be 33 kDa and 63 kDa, respectively. The two
proteases we ...
22nd EMC Full Program - 25th Enzyme Mechanisms Conference
... transduction. cMet and eight clinically relevant mutants were characterized by biochemical,
biophysical, and cellular methods. A model of RTK activation is proposed to describe how a
RTK response may be matched to a biological context through enzymatic properties. In
addition, two highly optimized c ...
A restriction enzyme or restriction endonuclease is an enzyme that cuts DNA at or near specific recognition nucleotide sequences known as restriction sites. Restriction enzymes are commonly classified into three types, which differ in their structure and whether they cut their DNA substrate at their recognition site, or if the recognition and cleavage sites are separate from one another. To cut DNA, all restriction enzymes make two incisions, once through each sugar-phosphate backbone (i.e. each strand) of the DNA double helix.These enzymes are found in bacteria and archaea and provide a defense mechanism against invading viruses. Inside a prokaryote, the restriction enzymes selectively cut up foreign DNA in a process called restriction; while host DNA is protected by a modification enzyme (a methyltransferase) that modifies the prokaryotic DNA and blocks cleavage. Together, these two processes form the restriction modification system.Over 3000 restriction enzymes have been studied in detail, and more than 600 of these are available commercially. These enzymes are routinely used for DNA modification in laboratories, and are a vital tool in molecular cloning.