Eram_SeyedMohammad - UWSpace
... I hereby declare that I am the sole author of this thesis. This is a true copy of the thesis, including any required final revisions, as accepted by my examiners. I understand that my thesis may be made electronically available to the public. ...
... I hereby declare that I am the sole author of this thesis. This is a true copy of the thesis, including any required final revisions, as accepted by my examiners. I understand that my thesis may be made electronically available to the public. ...
Full Text
... this mechanism were proposed by Gacesa (1987). Glucan polysaccharides can also be degraded by phosphorolysis by such enzymes as α-glucan phosphorylases (Withers et al. 1981). Eukaryotic enzymes that depolymerize polysaccharides predominantly utilize hydrolytic mechanisms while the lytic mechanism is ...
... this mechanism were proposed by Gacesa (1987). Glucan polysaccharides can also be degraded by phosphorolysis by such enzymes as α-glucan phosphorylases (Withers et al. 1981). Eukaryotic enzymes that depolymerize polysaccharides predominantly utilize hydrolytic mechanisms while the lytic mechanism is ...
Bioretrosynthetic Construction of a Non
... the past several years. In addition to Dr. Bachmann, I would also like to thank my Dissertation Committee members of Dr. Richard Armstrong, Dr. Tina Iverson, Dr. Lawrence Marnett and Dr. Carmello Rizzo for their valued discussions and for offering their time and energy in assisting my training as a ...
... the past several years. In addition to Dr. Bachmann, I would also like to thank my Dissertation Committee members of Dr. Richard Armstrong, Dr. Tina Iverson, Dr. Lawrence Marnett and Dr. Carmello Rizzo for their valued discussions and for offering their time and energy in assisting my training as a ...
Characterization of the regulatory function of the 46
... Arabidopsis Rubisco activase was recently shown to be regulated by redox changes in the larger (46-kDa) isoform specifically mediated by thioredoxin-f [Zhang and Portis (1999) Proc Natl Acad Sci USA 96: 9438–9443]. Reduction greatly increases the activity of the 46-kDa isoform and the native protein ...
... Arabidopsis Rubisco activase was recently shown to be regulated by redox changes in the larger (46-kDa) isoform specifically mediated by thioredoxin-f [Zhang and Portis (1999) Proc Natl Acad Sci USA 96: 9438–9443]. Reduction greatly increases the activity of the 46-kDa isoform and the native protein ...
Branched-chain amino acid restriction in Zucker
... 12 h light:dark cycle with ad libitum access to water and food for up to 15 weeks. Food intake and weight gain were monitored weekly. A nonfasting blood sample was taken after 9 weeks on the diets at 9AM to determine the genotype and dietary effect on circulating amino acid concentrations. Plasma an ...
... 12 h light:dark cycle with ad libitum access to water and food for up to 15 weeks. Food intake and weight gain were monitored weekly. A nonfasting blood sample was taken after 9 weeks on the diets at 9AM to determine the genotype and dietary effect on circulating amino acid concentrations. Plasma an ...
1 Chapter 1 Chemistry On The Pyrimidine Ring
... use a binuclear metal center (26). Lower metal content for dihydropyrimidase led early researchers to also conclude that it was a mononuclear zinc enzyme. This phenomenon is due to pH playing an important part in the metal binding to amidohydrolases. Both dihydroorotases and dihydropyrimidases requi ...
... use a binuclear metal center (26). Lower metal content for dihydropyrimidase led early researchers to also conclude that it was a mononuclear zinc enzyme. This phenomenon is due to pH playing an important part in the metal binding to amidohydrolases. Both dihydroorotases and dihydropyrimidases requi ...
E. Coli MurG: A Paradigm for a Superfamily of
... residues. The alignment shows that a relatively small number of residues are invariant across the range of MurG homologs (Fig. (5)). These residues are confined to five different regions, three of which are the glycine-rich loops that are proposed to be involved in binding the diphosphates of the su ...
... residues. The alignment shows that a relatively small number of residues are invariant across the range of MurG homologs (Fig. (5)). These residues are confined to five different regions, three of which are the glycine-rich loops that are proposed to be involved in binding the diphosphates of the su ...
Characterization of the cDNA and Gene Coding for the Biotin
... Ward (personal communication) and to the partia1 sequence reported in GenBank accession number L34413. The major differences between these three sequences are at the 5' and 3' noncoding ends. At the 5' end, the pBS-1 sequence is 122 nucleotides longer than the sequence shown in GenBank accession num ...
... Ward (personal communication) and to the partia1 sequence reported in GenBank accession number L34413. The major differences between these three sequences are at the 5' and 3' noncoding ends. At the 5' end, the pBS-1 sequence is 122 nucleotides longer than the sequence shown in GenBank accession num ...
Electrophoresis Basi..
... neutral pH’s are either basic or acidic depending upon their AA composition. Most proteins placed into basic conditions become negatively charged. Acidic conditions cause most proteins to develop a positive charge. ...
... neutral pH’s are either basic or acidic depending upon their AA composition. Most proteins placed into basic conditions become negatively charged. Acidic conditions cause most proteins to develop a positive charge. ...
physiological reviews
... In the courseof these processesthe food substancesare transformed into compounds with high energy bonds (I) which can contribute to the pool of pyrophosphate nucleotides. In theselatter compoundsenergy is stored in a form immediately available for the purpose of biosynthetic or mechanical work. Oxid ...
... In the courseof these processesthe food substancesare transformed into compounds with high energy bonds (I) which can contribute to the pool of pyrophosphate nucleotides. In theselatter compoundsenergy is stored in a form immediately available for the purpose of biosynthetic or mechanical work. Oxid ...
An Investigation of Acetobacter aceti N5
... indicate that the two do not differ significantly, indicating there has been no adaptive change in enzyme mechanism. Also reported is a summary and analysis of a number of crystal structures that have been determined for AaPurE, which suggests a strategy by which proteins may have become resistant t ...
... indicate that the two do not differ significantly, indicating there has been no adaptive change in enzyme mechanism. Also reported is a summary and analysis of a number of crystal structures that have been determined for AaPurE, which suggests a strategy by which proteins may have become resistant t ...
Purification and properties of NADP +-dependent
... batch prepared from between one to five residues per mole. In the purified A . niger enzyme one ornithine residue was found. The presence of ornithine was confirmed independently by the methanesulphonic acid and performic acid hydrolysis methods. The modification must be due to the action of an argi ...
... batch prepared from between one to five residues per mole. In the purified A . niger enzyme one ornithine residue was found. The presence of ornithine was confirmed independently by the methanesulphonic acid and performic acid hydrolysis methods. The modification must be due to the action of an argi ...
Quantitative Analysis of the Kinetics of End
... structure. The ATP concentration is maintained at high levels so that each DNA-bound RecA monomer is functioning at its kcat. The kcat employed is that observed in the presence of longer random sequence ssDNA molecules. Under conditions used in this series of experiments, the kcat for ATP hydrolysis ...
... structure. The ATP concentration is maintained at high levels so that each DNA-bound RecA monomer is functioning at its kcat. The kcat employed is that observed in the presence of longer random sequence ssDNA molecules. Under conditions used in this series of experiments, the kcat for ATP hydrolysis ...
Malonate decarboxylase of Pseudomonas putida is composed of
... acyl-carrier protein (ACP) being responsible for the reaction sequence on cyclic decarboxylation of mal- ...
... acyl-carrier protein (ACP) being responsible for the reaction sequence on cyclic decarboxylation of mal- ...
ATP production in brain and liver mitochondria of Fischer
... temperature of the larger assay volume of the reaction mixture (990 l), which is kept at 28°C. Like the standards, the background of the reaction mixtures (990 l) was recorded before the addition of mitochondria. Afterward, 10 l of freshly isolated mitochondria from the frontal cortex of the brai ...
... temperature of the larger assay volume of the reaction mixture (990 l), which is kept at 28°C. Like the standards, the background of the reaction mixtures (990 l) was recorded before the addition of mitochondria. Afterward, 10 l of freshly isolated mitochondria from the frontal cortex of the brai ...
Studies Into the Allosteric Regulation of ADP
... In glycogen, chains of α-1,4-polyglucans link with other chains to form a branching pattern. In mammals and yeast, glycogen synthesis requires a 'seed' of glycogenin, which is a self-glucosylating initiator protein, although no analogue of glycogen has been described in bacteria.4 Glycogen is a larg ...
... In glycogen, chains of α-1,4-polyglucans link with other chains to form a branching pattern. In mammals and yeast, glycogen synthesis requires a 'seed' of glycogenin, which is a self-glucosylating initiator protein, although no analogue of glycogen has been described in bacteria.4 Glycogen is a larg ...
Interactions of TCA cycle enzymes and of the CcpA
... has already been characterized in other organisms. Moreover, the enzymatic reaction of Mdh, the endergonic conversion of malate to oxaloacetate, is only driven by the fast conversion of oxaloacetate by CitZ. Since no direct interaction between Mdh and CitZ was detected, other factors, e.g. other TCA ...
... has already been characterized in other organisms. Moreover, the enzymatic reaction of Mdh, the endergonic conversion of malate to oxaloacetate, is only driven by the fast conversion of oxaloacetate by CitZ. Since no direct interaction between Mdh and CitZ was detected, other factors, e.g. other TCA ...
Purification and Characterization of Two Thermostable Proteases
... to homogeneity by fractional ammonium sulfate precipitation, ion-exchange chromatography on DEAE-Sepharose, and PhenylSepharose hydrophobic interaction chromatography. By SDSPAGE, the molecular mass of the two purified enzymes was estimated to be 33 kDa and 63 kDa, respectively. The two proteases we ...
... to homogeneity by fractional ammonium sulfate precipitation, ion-exchange chromatography on DEAE-Sepharose, and PhenylSepharose hydrophobic interaction chromatography. By SDSPAGE, the molecular mass of the two purified enzymes was estimated to be 33 kDa and 63 kDa, respectively. The two proteases we ...
22nd EMC Full Program - 25th Enzyme Mechanisms Conference
... transduction. cMet and eight clinically relevant mutants were characterized by biochemical, biophysical, and cellular methods. A model of RTK activation is proposed to describe how a RTK response may be matched to a biological context through enzymatic properties. In addition, two highly optimized c ...
... transduction. cMet and eight clinically relevant mutants were characterized by biochemical, biophysical, and cellular methods. A model of RTK activation is proposed to describe how a RTK response may be matched to a biological context through enzymatic properties. In addition, two highly optimized c ...