Plant aromatic amino acid decarboxylases
... categories remain almost identical. Due to this high sequence homology, it has historically been difficult to predict the function of any given plant AAAD through sequence comparison. This extensive homology has lead to a major problem in distinguishing activity and substrate specificity from a prim ...
... categories remain almost identical. Due to this high sequence homology, it has historically been difficult to predict the function of any given plant AAAD through sequence comparison. This extensive homology has lead to a major problem in distinguishing activity and substrate specificity from a prim ...
An Introduction to Enzyme Science
... the enzyme of interest from other contaminants (e.g., proteins, solutes, etc.) affecting the enzyme’s activity. In practice, absolute purity is not required as long as other contaminating enzymes and proteins are without effect on the enzyme of interest. It is helpful to apply the principles of orga ...
... the enzyme of interest from other contaminants (e.g., proteins, solutes, etc.) affecting the enzyme’s activity. In practice, absolute purity is not required as long as other contaminating enzymes and proteins are without effect on the enzyme of interest. It is helpful to apply the principles of orga ...
risk and technical assessment report
... were no concerns with the safety of B. circulans, when used as a source of β-galactosidase. Question 2: What is the risk to the public from the use of β-galactosidase derived from B. circulans as a processing aid? No safety concerns with the use of this enzyme as a processing aid were raised. The sa ...
... were no concerns with the safety of B. circulans, when used as a source of β-galactosidase. Question 2: What is the risk to the public from the use of β-galactosidase derived from B. circulans as a processing aid? No safety concerns with the use of this enzyme as a processing aid were raised. The sa ...
Taxonomic characterization of Ochrobactrum sp. isolates from soil
... cluster, a separate DNA–DNA reassociation group and showed, as a biological particularity, a strict preference for the rhizoplane as habitat. Diagnostic phenotypic characters were found. This indicated that REP group J strains represent a further new species, although phylogenetic analyses using 16S ...
... cluster, a separate DNA–DNA reassociation group and showed, as a biological particularity, a strict preference for the rhizoplane as habitat. Diagnostic phenotypic characters were found. This indicated that REP group J strains represent a further new species, although phylogenetic analyses using 16S ...
Autotrophic CO2 fixation via the reductive tricarboxylic acid cycle in
... to have been among the first types of organisms on Earth (e.g. Huber and Wächtershäuser, 1997; Russell and Hall, 1997). Phylogenetic analyses based on 16S rRNA and whole genomes place autotrophic hyperthermophiles at the base of the evolutionary tree of life, lending evidence to the hypothesis that ...
... to have been among the first types of organisms on Earth (e.g. Huber and Wächtershäuser, 1997; Russell and Hall, 1997). Phylogenetic analyses based on 16S rRNA and whole genomes place autotrophic hyperthermophiles at the base of the evolutionary tree of life, lending evidence to the hypothesis that ...
Physiological and Chemical Properties of a
... An inorganic pyrophosphatase was purified from Desulfuvibrio desulfuricans strain BERRE SOL. Its activity was increased up to 130-fold by reduction with reagents (Na2S20,, Na[BH,], Na,S, etc.) of Ei values less than -0.14V at pH 7.0 and 25'. The pure enzyme was isoelectric at pH 6.55 ;its molecular ...
... An inorganic pyrophosphatase was purified from Desulfuvibrio desulfuricans strain BERRE SOL. Its activity was increased up to 130-fold by reduction with reagents (Na2S20,, Na[BH,], Na,S, etc.) of Ei values less than -0.14V at pH 7.0 and 25'. The pure enzyme was isoelectric at pH 6.55 ;its molecular ...
Bacterial methionine biosynthesis
... Methionine is essential in all organisms, as it is both a proteinogenic amino acid and a component of the cofactor, S-adenosyl methionine. The metabolic pathway for its biosynthesis has been extensively characterized in Escherichia coli; however, it is becoming apparent that most bacterial species d ...
... Methionine is essential in all organisms, as it is both a proteinogenic amino acid and a component of the cofactor, S-adenosyl methionine. The metabolic pathway for its biosynthesis has been extensively characterized in Escherichia coli; however, it is becoming apparent that most bacterial species d ...
Fibrinogen Bern I: Substitution y 337 Asn + Lys Is
... functional defect caused by structural alteration in the fibrinogen molecule. Most dysfibrinogenemias have thus far been detected by virtue of a prolonged thrombin clotting time and, therefore, mainly represent variants with defective fibrinogen to fibrin conversion. More than 240 abnormal fibrinoge ...
... functional defect caused by structural alteration in the fibrinogen molecule. Most dysfibrinogenemias have thus far been detected by virtue of a prolonged thrombin clotting time and, therefore, mainly represent variants with defective fibrinogen to fibrin conversion. More than 240 abnormal fibrinoge ...
New peptide and gene coding for same
... RNA were extracted from human atrium cordis by a method disclosed in Chirgwin (Chirgwin, J. M. et. al., Biochemistry 18, 5294-5299, 1979), and enriched for poly (A)+ RNA (mRNA) with an oligo (dT) cellulose column. The poly (A) RNA were used to prepare a cDNA library according to the Okayama-Berg met ...
... RNA were extracted from human atrium cordis by a method disclosed in Chirgwin (Chirgwin, J. M. et. al., Biochemistry 18, 5294-5299, 1979), and enriched for poly (A)+ RNA (mRNA) with an oligo (dT) cellulose column. The poly (A) RNA were used to prepare a cDNA library according to the Okayama-Berg met ...
41 Purine and Pyrimidine Metabolism
... respectively. A primary site of regulation is the synthesis of PRPP. PRPP synthetase is negatively affected by GDP and, at a distinct allosteric site, by ADP. Thus, the simultaneous binding of an oxypurine (eg., GDP) and an aminopurine (eg., ADP) can occur with the result being a synergistic inhibit ...
... respectively. A primary site of regulation is the synthesis of PRPP. PRPP synthetase is negatively affected by GDP and, at a distinct allosteric site, by ADP. Thus, the simultaneous binding of an oxypurine (eg., GDP) and an aminopurine (eg., ADP) can occur with the result being a synergistic inhibit ...
File
... molecular level. The application of new experimental techniques for the isolation and manipulation of specific fragments of DNA has greatly increased our understanding of the processes responsible for the replication and expression of genetic information. Furthermore, the ability to isolate a specif ...
... molecular level. The application of new experimental techniques for the isolation and manipulation of specific fragments of DNA has greatly increased our understanding of the processes responsible for the replication and expression of genetic information. Furthermore, the ability to isolate a specif ...
Kinetic studies of human tyrosyl
... enzyme that relaxes DNA supercoiling and relieves torsional strain of DNA during replication, repair and transcription processes by making single stranded breaks on DNA, unwinding and religating the DNA ends in the cleaved strand [1]. During the process, DNA becomes covalently linked to Topo I via t ...
... enzyme that relaxes DNA supercoiling and relieves torsional strain of DNA during replication, repair and transcription processes by making single stranded breaks on DNA, unwinding and religating the DNA ends in the cleaved strand [1]. During the process, DNA becomes covalently linked to Topo I via t ...
Diiffusional correlations among multiple active sites in a single enzyme
... modification of the model where the enzyme–substrate interactions are removed, while still retaining binding interactions with the active site beads. The plots of g R(r) and g P(r) for this case are given in Fig. 5 and the lack of structure is evident. For this model the substrate molecules can free ...
... modification of the model where the enzyme–substrate interactions are removed, while still retaining binding interactions with the active site beads. The plots of g R(r) and g P(r) for this case are given in Fig. 5 and the lack of structure is evident. For this model the substrate molecules can free ...
BIOCHEMISTRY AND MOLECULAR BIOLOGY Problem Unit Two
... inhibitor (i.e., a bacterial toxin, a chemical, etc.), the overproduction of an enzyme, or the introduction of a foreign enzyme (i.e., viral infection). Enzymes are popular therapeutic targets. Pharmaceuticals are frequently enzyme inhibitors. Enzyme assays are important in diagnosis. A diminution o ...
... inhibitor (i.e., a bacterial toxin, a chemical, etc.), the overproduction of an enzyme, or the introduction of a foreign enzyme (i.e., viral infection). Enzymes are popular therapeutic targets. Pharmaceuticals are frequently enzyme inhibitors. Enzyme assays are important in diagnosis. A diminution o ...
Characterization of the chimeric seven
... pump that has been found in a variety of marine bacteria. Recently, many PR-like genes were found in non-marine environments. The goal of this study is to explore the function of rhodopsins that exist only as partial proteoopsin genes using chimeras with marine green PR (GPR). We isolated nine parti ...
... pump that has been found in a variety of marine bacteria. Recently, many PR-like genes were found in non-marine environments. The goal of this study is to explore the function of rhodopsins that exist only as partial proteoopsin genes using chimeras with marine green PR (GPR). We isolated nine parti ...
18. enzymes iii
... Frederick August Kekule (LT, 1829-1867), master of structural chemistry. After receiving his doctoral degree from the University of Strasbourg in 1874, he taught at the Universities of Erlangen and Wurzburg, eventually becoming Professor of Chemistry at the University of Berlin in 1892 while succeed ...
... Frederick August Kekule (LT, 1829-1867), master of structural chemistry. After receiving his doctoral degree from the University of Strasbourg in 1874, he taught at the Universities of Erlangen and Wurzburg, eventually becoming Professor of Chemistry at the University of Berlin in 1892 while succeed ...
Chondroitinase ABC I from Proteus vulgaris: cloning, recombinant
... GalAG, galactosaminoglycan; GalNAc, N -acetylgalactosamine; IdoA, iduronic acid; UA, 4,5 unsaturated uronic acid. ...
... GalAG, galactosaminoglycan; GalNAc, N -acetylgalactosamine; IdoA, iduronic acid; UA, 4,5 unsaturated uronic acid. ...
Nucleotide Metabolism
... OMP Decarboxylase is one of the Most Efficient Enzymes Known and Makes UMP CTP is Synthesized from UTP by CTP Synthetase CTP Synthetase Activated by GTP, Inhibited by CTP and Phosphorylation CTP Synthetase Helps to Balance Purines and Pyrimidines ...
... OMP Decarboxylase is one of the Most Efficient Enzymes Known and Makes UMP CTP is Synthesized from UTP by CTP Synthetase CTP Synthetase Activated by GTP, Inhibited by CTP and Phosphorylation CTP Synthetase Helps to Balance Purines and Pyrimidines ...