Glycerol transport and phosphoenolpyruvate
... and metabolism independent of PTS-mediated activation by phosphorylation. This could indeed be confirmed for the His-232-Arg mutant glycerol kinase of Ent. casseliflavus, which exhibited much higher activity than the wild-type enzyme (Charrier et al., 1997). An amino acid sequence comparison with kn ...
... and metabolism independent of PTS-mediated activation by phosphorylation. This could indeed be confirmed for the His-232-Arg mutant glycerol kinase of Ent. casseliflavus, which exhibited much higher activity than the wild-type enzyme (Charrier et al., 1997). An amino acid sequence comparison with kn ...
Identification and first characterization of pairing
... severe damage and chronic inflammation of the according tissues, as eggs secrete lytic enzymes and serologically active proteins (Ashton et al., 2001; Schramm & Haas, 2010). A number of eggs are accidentally transported via the blood stream to inner organs such as the liver and spleen, where they ca ...
... severe damage and chronic inflammation of the according tissues, as eggs secrete lytic enzymes and serologically active proteins (Ashton et al., 2001; Schramm & Haas, 2010). A number of eggs are accidentally transported via the blood stream to inner organs such as the liver and spleen, where they ca ...
The Bacterial Toxin RelE Displays Codon
... At a RelE concentration four times less than the concentration of ribosomes that were competent in dipeptide formation (Zavialov et al., 2001; Experimental Procedures), peptide synthesis from the mRNA with UAG as stop codon was completely blocked by the toxin. With twenty times less RelE than active ...
... At a RelE concentration four times less than the concentration of ribosomes that were competent in dipeptide formation (Zavialov et al., 2001; Experimental Procedures), peptide synthesis from the mRNA with UAG as stop codon was completely blocked by the toxin. With twenty times less RelE than active ...
Hormone sensitive lipase: structure, function and
... is located at 5' flanking region of exon B (Figure 6). Using truncated promoter transcripts, ranging from -2400 to -31, they determined that the 5' border of the minimal promoter is located between -86 and -57 (Figure 7). Remarkably, the HSL promoter does not contain a TATA-box or a CCAAT-box, conse ...
... is located at 5' flanking region of exon B (Figure 6). Using truncated promoter transcripts, ranging from -2400 to -31, they determined that the 5' border of the minimal promoter is located between -86 and -57 (Figure 7). Remarkably, the HSL promoter does not contain a TATA-box or a CCAAT-box, conse ...
General principles of cellular organization in the genome
... higher fraction of multifunctional enzymes compared to more complex bacteria; general features such as metabolite concentrations, cellular energetics, adaptability and global gene expression responses are similar though. For the transcriptome analysis we combined strand-specific tiling arrays, compl ...
... higher fraction of multifunctional enzymes compared to more complex bacteria; general features such as metabolite concentrations, cellular energetics, adaptability and global gene expression responses are similar though. For the transcriptome analysis we combined strand-specific tiling arrays, compl ...
Understanding the Regulation of Metabolic Enzyme Acetylation in E
... Global protein acetylation is a newly discovered phenomenon in bacteria. Of the more than 250 acetylations reported in E. coli, many are of metabolic enzymes [1-3]. Thus, acetylation could represent a novel posttranslational mechanism of metabolic control. Yet, almost nothing is known about the regu ...
... Global protein acetylation is a newly discovered phenomenon in bacteria. Of the more than 250 acetylations reported in E. coli, many are of metabolic enzymes [1-3]. Thus, acetylation could represent a novel posttranslational mechanism of metabolic control. Yet, almost nothing is known about the regu ...
Identification and characterization of novel interaction
... SPINE analysis of StrepCcpA ......................................................................................................... 73 ...
... SPINE analysis of StrepCcpA ......................................................................................................... 73 ...
Locked Nucleic Acid (LNA™)
... When ordering an LNA™ oligonucleotide from Exiqon you can either design the sequence and LNA™ spiking pattern yourself or let Exiqon’s experts help you with the design. Design your own LNA™ oligonucleotide Incorporation of LNA™ in a sequence strongly affects the properties of the oligonucleotide and ...
... When ordering an LNA™ oligonucleotide from Exiqon you can either design the sequence and LNA™ spiking pattern yourself or let Exiqon’s experts help you with the design. Design your own LNA™ oligonucleotide Incorporation of LNA™ in a sequence strongly affects the properties of the oligonucleotide and ...
Origin of the catalytic activity of bovine seminal ribonuclease against
... ABSTRACT: Bovine seminal ribonuclease (RNase) binds, melts, and (in the case of RNA) catalyzes the hydrolysis of double-stranded nucleic acid 30-fold better under physiological conditions than its pancreatic homologue, the well-known RNase A. Reported here are site-directed mutagenesis experiments t ...
... ABSTRACT: Bovine seminal ribonuclease (RNase) binds, melts, and (in the case of RNA) catalyzes the hydrolysis of double-stranded nucleic acid 30-fold better under physiological conditions than its pancreatic homologue, the well-known RNase A. Reported here are site-directed mutagenesis experiments t ...
Supplementary materials - Stanford Artificial Intelligence Laboratory
... Importantly, the candidate genetic regulators for Geronemo consist of both continuousvalued e-regulators and discrete-valued g-regulators. The continuous-valued candidate regulators have more possible split values than the discrete-valued ones; therefore it is possible that e-regulators can be captu ...
... Importantly, the candidate genetic regulators for Geronemo consist of both continuousvalued e-regulators and discrete-valued g-regulators. The continuous-valued candidate regulators have more possible split values than the discrete-valued ones; therefore it is possible that e-regulators can be captu ...
Short-term regulation of the mammalian pyruvate dehydrogenase
... molecules of TDP-containing pyruvate dehydrogenase (EC 1.2.4.1; abbreviated E1) are joined to the binding domains of E2 (Reed, 2001; Fries et al., 2003). Twelve homodimeric molecules of FAD-containing dihydrolipoamide dehydrogenase (EC 1.8.1.4; abbreviated E3) are connected with the binding domains ...
... molecules of TDP-containing pyruvate dehydrogenase (EC 1.2.4.1; abbreviated E1) are joined to the binding domains of E2 (Reed, 2001; Fries et al., 2003). Twelve homodimeric molecules of FAD-containing dihydrolipoamide dehydrogenase (EC 1.8.1.4; abbreviated E3) are connected with the binding domains ...
Cells: building blocks of living organisms
... sickness) and Homo sapiens (multicellular organism) Archaea: (archaebacteria) like the prokaryotes they lack the nuclear membrane but have transcription and translation mechanisms close to those of the eukaryotes ...
... sickness) and Homo sapiens (multicellular organism) Archaea: (archaebacteria) like the prokaryotes they lack the nuclear membrane but have transcription and translation mechanisms close to those of the eukaryotes ...
Monoclonal Antibodies Specific for Oxidative Phosphorylation
... * Species specificity: Hum, human; Bov, bovine; Mou, mouse; (+), strong reactivity; (±), weak reactivity; -(), no reactivity. When these monoclonal antibodies are used in immunohistochemistry applications, formaldehyde-fixed target cells may require antigen-retrieval methods before the target cell ...
... * Species specificity: Hum, human; Bov, bovine; Mou, mouse; (+), strong reactivity; (±), weak reactivity; -(), no reactivity. When these monoclonal antibodies are used in immunohistochemistry applications, formaldehyde-fixed target cells may require antigen-retrieval methods before the target cell ...
Investigating the link between tRNA and mRNA - EMBL-EBI
... The genetic code describes how a sequence of codons on an mrna is translated into a sequence of amino acids, forming a protein. The genetic code manifests itself in the cell as trna molecules, which fall into several classes of anticodon isoacceptors, each decoding a single codon into its correspond ...
... The genetic code describes how a sequence of codons on an mrna is translated into a sequence of amino acids, forming a protein. The genetic code manifests itself in the cell as trna molecules, which fall into several classes of anticodon isoacceptors, each decoding a single codon into its correspond ...
Molecular analysis and biological implications of STAT3 signal
... in relation to the negative charge at position 727. A model is presented in figure 3. The coactivator protein p300 associates with STAT3 in vivo upon IL-6 stimulation, and p300 strongly associates with the minimal TAD of STAT3 when serine 727 is mutated into aspartate (Chapter 4). The function of th ...
... in relation to the negative charge at position 727. A model is presented in figure 3. The coactivator protein p300 associates with STAT3 in vivo upon IL-6 stimulation, and p300 strongly associates with the minimal TAD of STAT3 when serine 727 is mutated into aspartate (Chapter 4). The function of th ...
- VU Research Repository
... example, many genes associated with protein biosynthesis were more highly expressed, as were pyruvate decarboxylase genes. Interestingly, however, there was no significant increase in the expression of trehalose synthesis genes or genes encoding HSPs; genes which, in previous studies, appeared to be ...
... example, many genes associated with protein biosynthesis were more highly expressed, as were pyruvate decarboxylase genes. Interestingly, however, there was no significant increase in the expression of trehalose synthesis genes or genes encoding HSPs; genes which, in previous studies, appeared to be ...
AraC Protein, Regulation of the L-arabinose Operon in Escherichia
... intermediate concentrations of arabinose. The crystal structure of the dimerization domain of AraC with arabinose bound reveals that the binding of arabinose generates an intricate network of hydrogen bonds between arabinose and residues lining the binding pocket (Soisson et al., 1997). Analysis of ...
... intermediate concentrations of arabinose. The crystal structure of the dimerization domain of AraC with arabinose bound reveals that the binding of arabinose generates an intricate network of hydrogen bonds between arabinose and residues lining the binding pocket (Soisson et al., 1997). Analysis of ...
Cardiac mitochondrial matrix and respiratory complex - AJP
... proteome that are yet to be established as functionally significant, rather than mining for PTMs as sources of demonstrated alterations in extracted enzyme molar activities. This reversed strategy may be an inefficient approach for many reasons. First, many protein amino acids, and likely associated ...
... proteome that are yet to be established as functionally significant, rather than mining for PTMs as sources of demonstrated alterations in extracted enzyme molar activities. This reversed strategy may be an inefficient approach for many reasons. First, many protein amino acids, and likely associated ...
The deleterious effect of missense mutations on pre
... ABSTRACT The presence of missense mutations detected during genetic testing makes it difficult to classify their pathogenic effect. It is possible that the predicted amino acid change affects protein function; however, it is also possible that a missense mutation does not act at the protein level b ...
... ABSTRACT The presence of missense mutations detected during genetic testing makes it difficult to classify their pathogenic effect. It is possible that the predicted amino acid change affects protein function; however, it is also possible that a missense mutation does not act at the protein level b ...
+ΔFosB
... - Nave is the mean N value for the control - Ctave is the mean Ct value for the control. - Fold differences (stress ChIP relative to control ChIP) were then determined by raising 2 to the Ct power. Input : One hundred microliters of the pre-immunoprecipitated lysate Control ChIP : samples were immun ...
... - Nave is the mean N value for the control - Ctave is the mean Ct value for the control. - Fold differences (stress ChIP relative to control ChIP) were then determined by raising 2 to the Ct power. Input : One hundred microliters of the pre-immunoprecipitated lysate Control ChIP : samples were immun ...
In situ hybridization
... Oligonucleotide gene probes have multiple advantages over RNA or cDNA probes when used for in situ hybridization. We can list them here for you. Stability, Availability, Faster and less expensive to use, Easier to work with, More specific, Better tissue penetration, Better reproducibility and a wide ...
... Oligonucleotide gene probes have multiple advantages over RNA or cDNA probes when used for in situ hybridization. We can list them here for you. Stability, Availability, Faster and less expensive to use, Easier to work with, More specific, Better tissue penetration, Better reproducibility and a wide ...
Origins and Early Evolution of the tRNA Molecule
... region (half domain of tRNA with the amino acid attachment site) interacts with the conserved domain of aaRSs for amino acid activation. The other tRNA half interacts with the non-conserved domain of aaRSs for specific recognition of an anticodon, although there are some exceptions, including tRNASe ...
... region (half domain of tRNA with the amino acid attachment site) interacts with the conserved domain of aaRSs for amino acid activation. The other tRNA half interacts with the non-conserved domain of aaRSs for specific recognition of an anticodon, although there are some exceptions, including tRNASe ...
Saccharomyces cerevisiae.
... However, there are still organisms that can grow anaerobically, and even those that can survive under both conditions. The question is what the difference is between these organisms. Why can some grow only in the presence of molecular oxygen, some only in the absence, and are some able to withstand ...
... However, there are still organisms that can grow anaerobically, and even those that can survive under both conditions. The question is what the difference is between these organisms. Why can some grow only in the presence of molecular oxygen, some only in the absence, and are some able to withstand ...
709_2010_211_MOESM2_ESM - Springer Static Content Server
... RESPONSE REGULATOR 6: Encodes a Type-A response regulator that is responsive to cytokinin treatment. Its C-ter domain is very short in comparison to other Arabidopsis ARRs (17 total). Arr6 protein is stabilized by cytokinin. Process: Cytokinin mediated signaling pathway (Hwang et al. 2002; To et al. ...
... RESPONSE REGULATOR 6: Encodes a Type-A response regulator that is responsive to cytokinin treatment. Its C-ter domain is very short in comparison to other Arabidopsis ARRs (17 total). Arr6 protein is stabilized by cytokinin. Process: Cytokinin mediated signaling pathway (Hwang et al. 2002; To et al. ...