The use of glycogen and GlycoBlue reagent in Qubit DNA and RNA
... The use of glycogen and GlycoBlue reagent in Qubit DNA and RNA assays as measured on the Qubit Fluorometer Glycogen (a branched-chain carbohydrate; Cat. No. 10814010) and Invitrogen™ GlycoBlue™ Coprecipitant (glycogen covalently linked to a blue dye; Cat. No. AM9515) are reagents commonly used to fa ...
... The use of glycogen and GlycoBlue reagent in Qubit DNA and RNA assays as measured on the Qubit Fluorometer Glycogen (a branched-chain carbohydrate; Cat. No. 10814010) and Invitrogen™ GlycoBlue™ Coprecipitant (glycogen covalently linked to a blue dye; Cat. No. AM9515) are reagents commonly used to fa ...
Slide 1
... and analysis of rare mRNA transcripts or other RNA present in low abundance. RNA cannot serve as a template for PCR, so it must be first transcribed into cDNA with reverse transcriptase from Moloney murine leukemia virus or Avian myeloblastosis virus, and the cDNA copy is then amplified. ...
... and analysis of rare mRNA transcripts or other RNA present in low abundance. RNA cannot serve as a template for PCR, so it must be first transcribed into cDNA with reverse transcriptase from Moloney murine leukemia virus or Avian myeloblastosis virus, and the cDNA copy is then amplified. ...
Avian Infectious Bronchitis Virus (IBV) genesig Standard Kit
... All kinds of sample material suited for PCR amplification can be used. Please ensure the samples are suitable in terms of purity, concentration, and RNA/DNA integrity. Always run at least one negative control with the samples. To prepare a negative-control, replace the template RNA sample with RNAse ...
... All kinds of sample material suited for PCR amplification can be used. Please ensure the samples are suitable in terms of purity, concentration, and RNA/DNA integrity. Always run at least one negative control with the samples. To prepare a negative-control, replace the template RNA sample with RNAse ...
Avian Infectious Bronchitis Virus (IBV)
... amplification, forward and reverse primers hybridize to the IBV cDNA. A fluorogenic probe is included in the same reaction mixture which consists of a DNA probe labeled with a 5`dye and a 3`-quencher. During PCR amplification, the probe is cleaved and the reporter dye and quencher are separated. The ...
... amplification, forward and reverse primers hybridize to the IBV cDNA. A fluorogenic probe is included in the same reaction mixture which consists of a DNA probe labeled with a 5`dye and a 3`-quencher. During PCR amplification, the probe is cleaved and the reporter dye and quencher are separated. The ...
The Basics of RT-PCR
... tubes in the same run. This type of contamination is thus insidious, because the negative control tubes are often “clean;” therefore, unexpected positive results can be caused simply by contamination. If a contamination problem is suspected, it is sometimes helpful to perform multiple negative-contr ...
... tubes in the same run. This type of contamination is thus insidious, because the negative control tubes are often “clean;” therefore, unexpected positive results can be caused simply by contamination. If a contamination problem is suspected, it is sometimes helpful to perform multiple negative-contr ...
RNA–Amino Acid Binding - University of Colorado-MCDB
... such interactions are probable, independent of mooring of the amino acid to the RNA site via an adenosine residue, as occurs in SAM aptamer structures (e.g., in Fig. 1 above). Of course, even the aliphatic sections of side chains can interact with nucleobases by van der Waals and hydrophobic (entrop ...
... such interactions are probable, independent of mooring of the amino acid to the RNA site via an adenosine residue, as occurs in SAM aptamer structures (e.g., in Fig. 1 above). Of course, even the aliphatic sections of side chains can interact with nucleobases by van der Waals and hydrophobic (entrop ...
Microenvironment analysis and identification of magnesium binding
... the electrostatic ®eld (44). We believe that Mg2+ binding sites can be differentiated not only by charge alone, but also by the biochemical and structural properties surrounding the binding site. We used FEATURE to study the differences between site-bound and diffusely bound Mg2+ ions in complex RNA ...
... the electrostatic ®eld (44). We believe that Mg2+ binding sites can be differentiated not only by charge alone, but also by the biochemical and structural properties surrounding the binding site. We used FEATURE to study the differences between site-bound and diffusely bound Mg2+ ions in complex RNA ...
D-Isonucleotide (isoNA) incorporation around cleavage site of
... frequencies. To confirm the open-close motion, we determined the distance between the most flexible residue in the PAZ loop and the active site. The open-close ranges vary from 30 Å to 57 Å along this indicator (Fig. 3C) for the Ago_Native model. A similar motion was also observed for the Ago_S08D m ...
... frequencies. To confirm the open-close motion, we determined the distance between the most flexible residue in the PAZ loop and the active site. The open-close ranges vary from 30 Å to 57 Å along this indicator (Fig. 3C) for the Ago_Native model. A similar motion was also observed for the Ago_S08D m ...
Translation Tutorial
... A process called transcription starts in the nucleus, where an enzyme called RNA polymerase splits the DNA molecule. Next, free floating mRNA nucleotides bond to the open DNA molecule. next Once finished, the mRNA breaks away and exits the nucleus. The mRNA will then join a ribosome. Now, the proces ...
... A process called transcription starts in the nucleus, where an enzyme called RNA polymerase splits the DNA molecule. Next, free floating mRNA nucleotides bond to the open DNA molecule. next Once finished, the mRNA breaks away and exits the nucleus. The mRNA will then join a ribosome. Now, the proces ...
Cell-Free Protein Expression Systems
... In RRL translation reactions, mRNA is used as template for translation. In general, optimal results will be achieved after an incubation time of 1.5 hours at 30°C. However, many template-related factors affect translation efficiency of specific mRNAs in the RRL system and should be considered when d ...
... In RRL translation reactions, mRNA is used as template for translation. In general, optimal results will be achieved after an incubation time of 1.5 hours at 30°C. However, many template-related factors affect translation efficiency of specific mRNAs in the RRL system and should be considered when d ...
Cloning and characterization of the Xenopus laevis p8 gene
... it is likely to be a transcription factor. The DNAbinding activity of human p8 is increased with phosphorylation (Encinar et al. 2001) and, while we could not identify any putative phosphorylation sites on Xp8, there were a couple of well-conserved amino acids outside the bHLH region (Fig. 2a) that ...
... it is likely to be a transcription factor. The DNAbinding activity of human p8 is increased with phosphorylation (Encinar et al. 2001) and, while we could not identify any putative phosphorylation sites on Xp8, there were a couple of well-conserved amino acids outside the bHLH region (Fig. 2a) that ...
Review Evolution of the coordinate regulation of glycolytic enzyme
... 2001). At least eight out of the twelve functually distinct glycolytic enzyme genes are coordinately induced by hypoxia in mammalian cells (Webster, 1987; Webster et al., 1990; Webster and Murphy, 1988). The regulation involves contributions from at least four separate molecular pathways, some of wh ...
... 2001). At least eight out of the twelve functually distinct glycolytic enzyme genes are coordinately induced by hypoxia in mammalian cells (Webster, 1987; Webster et al., 1990; Webster and Murphy, 1988). The regulation involves contributions from at least four separate molecular pathways, some of wh ...
Computational Biology - Bioinformatik
... miRNAs can have dual oncogenic and tumour suppressive roles in cancer depending on the cell type and pattern of gene expression. Approximately 50% of all annotated human miRNA genes are located in fragile sites or areas of the genome that are associated with cancer. → Mutations in miRNAs or their bi ...
... miRNAs can have dual oncogenic and tumour suppressive roles in cancer depending on the cell type and pattern of gene expression. Approximately 50% of all annotated human miRNA genes are located in fragile sites or areas of the genome that are associated with cancer. → Mutations in miRNAs or their bi ...
Reverse Transcription PCR (RT-PCR): The Molecular
... or LTRs (see Figure 2). The integrated DNA is called proviral DNA or the provirus. The provirus enters a period of latency that can last for several years. The proviral DNA is replicated along with the cellular DNA and can be inherited through many generations. The HIV proviral DNA contains the majo ...
... or LTRs (see Figure 2). The integrated DNA is called proviral DNA or the provirus. The provirus enters a period of latency that can last for several years. The proviral DNA is replicated along with the cellular DNA and can be inherited through many generations. The HIV proviral DNA contains the majo ...
WebMOTIFS: Web-based integrated motif discovery
... similar motifs, WebMOTIFS clusters the significant motifs together and computes a single representative motif for each cluster. WebMOTIFS also provides the option of Bayesian motif discovery with THEME. The THEME algorithm searches for motifs consistent with proteins from specified DNA-binding domai ...
... similar motifs, WebMOTIFS clusters the significant motifs together and computes a single representative motif for each cluster. WebMOTIFS also provides the option of Bayesian motif discovery with THEME. The THEME algorithm searches for motifs consistent with proteins from specified DNA-binding domai ...
Electrochemical detection of polymerase reactions by specific metal
... such as triphosphates, DNA, pyrophosphate, and phosphate, albeit with different affinities (Spangler et al. Ann. N. Y. Acad. Sci. 2008. 1130:138-48.). These metal ions can be used either free or in complex with certain ligands that may increase their specificity. So far, these measurements were cond ...
... such as triphosphates, DNA, pyrophosphate, and phosphate, albeit with different affinities (Spangler et al. Ann. N. Y. Acad. Sci. 2008. 1130:138-48.). These metal ions can be used either free or in complex with certain ligands that may increase their specificity. So far, these measurements were cond ...
Nutritional Control of Growth and Development in Yeast
... simply by activating this pathway. Similarly, blocking signaling through the pathway concurrent with glucose addition eliminates most, albeit not all, of the responses. Thus, the PKA pathway is both necessary and sufficient for a majority of the transcriptional responses of the cell to glucose (Zama ...
... simply by activating this pathway. Similarly, blocking signaling through the pathway concurrent with glucose addition eliminates most, albeit not all, of the responses. Thus, the PKA pathway is both necessary and sufficient for a majority of the transcriptional responses of the cell to glucose (Zama ...
The promiscuous primase
... DNA primase has a pivotal role in DNA synthesis by making a short oligoribonucleotide that acts as a primer for DNA polymerase. Thus, the primase is essential for both leading and lagging strand synthesis. In principle the primase needs to act once only on the leading strand, but is required for the ...
... DNA primase has a pivotal role in DNA synthesis by making a short oligoribonucleotide that acts as a primer for DNA polymerase. Thus, the primase is essential for both leading and lagging strand synthesis. In principle the primase needs to act once only on the leading strand, but is required for the ...
Gene Therapy and Transgenic Animals
... of sequence the minimum ribozyme structure into and plasmids introduction 1. Identification Ribozyme-coding has been incorporated and of chemical modifications that retain ribozyme activity and administered, in effect ribozyme gene therapy. enhancing stability to nucleases. ...
... of sequence the minimum ribozyme structure into and plasmids introduction 1. Identification Ribozyme-coding has been incorporated and of chemical modifications that retain ribozyme activity and administered, in effect ribozyme gene therapy. enhancing stability to nucleases. ...
Can the Origin of the Genetic Code Be Explained - BIO
... particular amino acids with which they are associated in the modern genetic code – thus enabling the code to arise directly from stereochemical associations. Yarus found support for this thesis in an early experiment [4], in which he discovered a differential bonding affinity between the amino acid ...
... particular amino acids with which they are associated in the modern genetic code – thus enabling the code to arise directly from stereochemical associations. Yarus found support for this thesis in an early experiment [4], in which he discovered a differential bonding affinity between the amino acid ...
Friesland Foods Normal
... established binding motifs of regulator-protein family members were taken into account to define the actual binding motif. In addition, we defined the motifs such that they obey general constraints imposed by the molecular nature of the binding process and the helical nature of the DNA molecule. Sin ...
... established binding motifs of regulator-protein family members were taken into account to define the actual binding motif. In addition, we defined the motifs such that they obey general constraints imposed by the molecular nature of the binding process and the helical nature of the DNA molecule. Sin ...
Sequential Expression of Macromolecule
... during the growth phase (post-translational control) or they are formed in a late phase subsequent to growth (transcriptional control). In most cases studied, the enzymes involved in antibiotic synthesis are repressed during the growth phase (Walker & Hnilica, 1964; Pruess & Johnson, 1967; Gallo & K ...
... during the growth phase (post-translational control) or they are formed in a late phase subsequent to growth (transcriptional control). In most cases studied, the enzymes involved in antibiotic synthesis are repressed during the growth phase (Walker & Hnilica, 1964; Pruess & Johnson, 1967; Gallo & K ...
tRNA
... conjugate in a process referred to as “aminoacylation” - How should the tRNA attached to the following amino acids be nomenclatured (or named)? Arg, Glu, and Trp - On the other hand, tRNA cognates for corresponding amino acids are usually post-superscripted with the three-letter amino acid code—eg t ...
... conjugate in a process referred to as “aminoacylation” - How should the tRNA attached to the following amino acids be nomenclatured (or named)? Arg, Glu, and Trp - On the other hand, tRNA cognates for corresponding amino acids are usually post-superscripted with the three-letter amino acid code—eg t ...
Chromatin Condensing Functions of the Linker Histone C
... order transitions concomitant with binding to their macromolecular targets (9, 21, 22). While much progress has been made predicting the prevalence of intrinsically disordered protein domains in eukaryotic proteomes (23), there is a relative paucity of experimental biochemical data that address the ...
... order transitions concomitant with binding to their macromolecular targets (9, 21, 22). While much progress has been made predicting the prevalence of intrinsically disordered protein domains in eukaryotic proteomes (23), there is a relative paucity of experimental biochemical data that address the ...
EPICENTRE Enzyme Catalog
... Polymerase is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity f ...
... Polymerase is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity f ...