Download Exam Review 4B - Iowa State University

BIO 313 Exam Review 4B
Supplemental Instruction
Iowa State University
Leader:
Course:
Instructor:
Date:
Laura
Bio 313
Dr. Rodermel
11/15/15
Complete the following table:
Lac Operon Mutation
Lactose Present
Beta-Gal
Permease
Lactose Absent
Beta-Gal
Permease
lacI+ lacP+ lacO+ lacZ- lacY+
lacI- lacP+ lacO+ lacZ+ lacYlacI+ lacP- lacOc lacZ+ lacY+
lacI+ lacP+ lacOc lacZ- lacY+
lacI- lacP+ lacO+ lacZ- lacY- /
lacI+ lacP- lacO+ lacZ+ lacY+
lacI+ lacP- lacO+ lacZ+ lacY- /
lacI+ lacP+ lacOc lacZ- lacY+
1. Match the definition to corresponding term below:
miRNAs
Enzyme that cleaves hairpins from dsRNA and
gives rise to small miRNAs (22 nucleotides
long)
Primary miRNA transcript
A large multisubunit, ribonucleoprotein
complex that builds miRNA
Dicer
Come from dsRNA (ex. ds RNA viruses, some
mRNAs that can form hairpins)
RNA-induced silencing complex (RISC)
Arise from specific genes (~450 different
genes in humans)
siRNAs
Has long inverted repeats that form hairpins
2. Which of the following are responsible for forming the sex pili?
a. Conjugation
b. Enhancers
c. Fertility Factor
d. Kineticore and Microtubules
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3. In a partial diploid cell the second copy of the lac operon is found on the
a. Bacterial chromosome
b. F factor
c. Regulator Region
d. Plasmid
4. Which of the following components of the lac operon forms a trans-acting product
a. LacA
b. LacI
c. LacP
d. LacO
5. All of the following are structural genes except
a. LacZ
b. LacA
c. LacO
d. LacY
6. If lacO is mutated to lacOc, what is the result
a. The structural genes are not expressed
b. The RNA polymerase is unable to bind
c. The repressor binds the operator
d. The structural genes are always expressed
7. Which of the following is the correct relationship between glucose and catabolite
repression
a. High glucose, high levels of cAMP
b. High glucose, high levels of CAP
c. Low glucose, low levels of cAMP
d. Low glucose, high levels of cAMP
8. When the cAMP-CAP complex is bound which of the following takes place?
a. Polymerase binds the lacP more efficiently
b. Polymerase if unable to bind to the lacP
c. The repressor is unable to bind the operator
d. The lacP is mutated
9. All of the following are differences between eukaryotic and prokaryotic gene regulation
a. Eukaryotes have mRNA processing
b. Eukaryotes use histones and chromatin remodeling
c. Eukaryotes have non-coupled tx and tl
d. Eukaryotes have operons
10. Histone modification result from
a. A neutralization of negative charges from DNA
b. A neutralization of positive charges from DNA
c. A neutralization of negative charges from histones
d. A neutralization of positive charges from histones
11. Which of the following closes chromatin confirmation?
a. Acetylation
b. Deacetylation
c. Tx Activation
d. Non-methylated DNA
12. How do eukaryotes coordinate gene expression?
a. Operons
b. Enhancers
c. Response Elements
d. Regulator Proteins
13. Which of the following is located furthest upstream?
a. Enhancers
b. Core Promoter
c. Regulatory Promoter
d. Structural Genes
14. Which of the following is not a way that repressors act?
a. Compete with activators
b. Bind to the activator
c. Bind the silencer
d. Allow for assembly of basal tx apparatus
15. Which of the following is the function of an insulator?
a. Blocks all enhancers
b. Allows tx of all genes
c. Allows for specificity of gene expression
d. Blocks the effects of all promoters
16. Where are AU-rich elements located and what is its effect?
a. 5’ UTR, rapid degradation
b. 5’ UTR, slow degradation
c. 3’ UTR, rapid degradation
d. 3’ UTR, slow degradation
17. RNAi likely evolved from
a. DNA plasmid
b. DNA virus
c. RNA virus
d. Protein products
18. This phase consists of growth and protein production to allow for cell division
a. M Phase
b. G2 Phase
c. S Phase
d. G1 Phase
19. The attachment point for spindle microtubules is the
a. Telomere
b. Centromere
c. Origin of Replication
d. Homologous Pair
20. Draw out how miRNA and siRNAs function to inhibit translation?
miRNAs
21. Which phase of mitosis is depicted below?
e.
f.
g.
h.
Anaphase
Telophase
Metaphase
Prophase
22. Fill out the following table:
siRNAs
23. A parent cell is 2n with a haploid number of 4, draw this cell from Prophase I to the four
gametes that will be formed
24. Two parents are AA x aa, cross these individuals through to an F2 generation (include
phenotypic ratios)
25. List the three postulates for Mendel’s law of segregation
a.
b.
c.
26. The allele G (gray) is dominant to allele g (white) in rabbit coat color. What would the
resulting genotypic and phenotypic ratios be if a Gg was crossed with a white rabbit?
27. Define the two rules:
a. Addition
b. Multiplication