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February 19, 2010
Multiple choice questions
(numbers in brackets indicate the number of correct answers)
1. Which of the following statements are true
Transcriptomes consist of functional RNAs
Genes can be disrupted by RNA interference
The genetic code includes 1 termination codon
3’ ends of genes can be identified by rapid amplification of cDNA ends (RACE)
 A northern analysis involves separating DNA fragments in agarose gels
RNA interference does not work in prokaryotes
Homologous recombination can be used to inactivate genes
Transposons can be directed to disrupt specific genes
A Southern hybridization identifies genes that are expressed
Exon-intron boundaries can be easily located by a computer
Open reading frames are only found in protein-coding genes
2. Dideoxynucleotides are used in
PCR
Southern hybridization
Transformation
Cloning
DNA sequencing
Culturing of bacteria
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(1)
3. Protein-coding genes can be identified by
Transposon tagging
ORF scanning
Zoo-blotting
Nuclease S1 mapping
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(1)
4. The function of genes can be determined by
Gene inactivation
Homology search
Exon trapping
Zoo-blotting
Northern analysis
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(2)
5. Expression of genes can be analyzed by
Northern analysis
Southern analysis
Comparative genomics
RNA interference
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(1)
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(4)
2
6. Chromosome walking
Is used in genetic mapping
Can be used to close physical sequence gaps
Occurs in mitosis
Requires a genomic DNA library
Can be done by PCR
Is used in fluorescent in situ hybridization (FISH)
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7. Optical mapping
Uses restriction enzymes
Is normally done with metaphase chromosomes
Determines the position of restriction sites in a DNA molecule
Uses a fluorescent dye
Is a technique used in genetic mapping
8. Radiation hybrids
Are human cell lines
Can hold large pieces of chromosomal DNA
Are rodent cell lines
Are produced by irradiation with UV light
Have been used in mapping the yeast genome
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9. Physical mapping
Requires large numbers of organisms
Utilizes genomic libraries
Is less accurate than genetic mapping
Detects polymorphic DNA sequences
Uses restriction fragment polymorphisms (RFLPs)
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(2)
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(2)
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10. ‘Physical gaps’ in a DNA sequence
Are easier to close than ‘sequence gaps’
Are usually smaller than 100 bp
Can only be closed by making a new clone library
Can only be closed by sequencing all clones in a library a second time
11. An EST (Expressed Sequence Tag)
Is a protein sequence
Is an RNA sequence
Is a DNA sequence
Is used to delineate regulatory sequence elements
Is used in genetic mapping
Consists only of exon sequences
Can often be detected by histochemical assays
12. An STS (Sequence Tagged Site)
(3)
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(1)
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(1)
(2)
3
Is a protein sequence
Is an RNA sequence
Is a DNA sequence
Is used in genetic mapping
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(1)
13. Proteomes
Occur in all living cells
Consist of protein genes
Are characterized by Southern analysis
Differ in different cell types
Are analyzed by agarose gel electrophoresis
14. cDNA
Is made from ribosomal RNA
Is made from tRNA
Is made from mRNA
Contains only introns
Contains only exons
Is single-stranded
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(2)
15. ORF scanning
Is used to find exons
Is used to find intergenic sequences
Is used to find gene homologies
Is used to find protein-coding genes
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16. Clone fingerprinting
Is a cloning technique
Identifies overlapping DNA sequences
Is used in physical mapping of genomes
Is used in sequence assembly
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17. A codon bias
Is used in genome mapping
Is found in intergenic regions
Is found in functional RNAs
Is only found in eukaryotes
Is used to identify genes
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(1)
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Correct answers
26-29
23-25
18-22
14-17
12-13
<12

(2)
(1)
Total:
Mark
A
B
C
D
E
F
(2)
29