Download Exam 3 Review A - Iowa State University

BIO 313
Exam Review 3A
Supplemental Instruction
Iowa State University
Leader:
Course:
Instructor:
Date:
Laura
BIO 313
Dr. Rodermel
10/21/15
1. The concept that the sequence of codons in the DNA of a gene has a direct
correspondence with the sequence of amino acids in the protein is known as:
a. Translation
b. Coupled
c. Introns
d. Colinearity
2. Which of the following is not a principle element of prokaryotic mRNA?
a. 3’UTR
b. Promoter
c. 5’ UTR
d. Shine-Dalgarno sequence
3. Spliceosome-mediated splicing requires ___________
a. Exons splice sites
b. Intron consensus sequences
c. Shine-Dalgarno sequence
d. 5’ Cap
4. What type of RNA is critical to the function of the spliceosome?
a. tRNA
b. rRNA
c. snRNA
d. mRNA
5. RNA editing includes all of the following except
a. Addition of a nucleotide
b. Deletion of a nucleotide
c. Modification of a nucleotide
d. Recombination of a nucleotide
6. What is the one gene one enzyme (protein) hypothesis
a. There are 64 total codons
b. One gene encodes a single polypeptide
c. Each codon encodes only one amino acid
d. There are specific stop codons
7. Which statement describes isoaccepting tRNAs?
a. tRNAs that differ in the third nucleotide position of the codon
b. tRNAs that have more than a single amino acid attached
c. tRNAs that different anticodon sequences but accept the same amino acid
d. tRNAs that different anticodon sequences but accept different amino acids
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8. How many sense codons are there?
a. 20
b. 64
c. 3
d. 61
9. This helps set the reading frame for translation
a. Shine-Dalgarno sequence
b. Kozak sequence
c. Initiation codon
d. 5’ cap
10. Initiation factor 3 is responsible for
a. Promotes dissociation of the ribosome subunits
b. Preventing the large subunit from associating with the small subunit
c. Responsible for binding GTP
d. Deliver fMet to the initiator codon
11. Eukaryotic translation initiation is the same as prokaryotic in that it has/uses
a. Initiaiton factors
b. Shine-Dalgarno sequence
c. Same ribosomes
d. fMet-tRNA
12. During translation elongation in prokaryotes what is responsible for binding to the
charged tRNA?
a. EF-Tu
b. EF-Ts
c. EF-G
d. GTP
13. If translocation cannot occur, what likely has a mutation? Would this be a lethal
mutation?
a. EF-Tu, yes
b. EF-G, yes
c. RF-2, no
d. EF-G, no
14. Stage of translation defined by the assembly of necessary components at the ribosome
a. tRNA charging
b. Initiation
c. Elongation
d. Termination
15. The function of the Shine Dalgarno sequence
a. Binds IF-3
b. Associates to the large subunit of the ribosome
c. Binds to the 16S rRNA
d. Correctly positions the ribosome over the initiation codon
16. GTP binds to all of the following except
a. IF-2
b. EF-Tu
c. RF-3
d. EF-Ts
17. Proteins that fold the nascent polypeptide into its correct conformation for activity
a. RF-2
b. Molecular chaperone
c. Ubiquitin
d. Puromycin
18. What is the purpose of gene regulation?
a. Modify the mRNA sequence
b. Produce precise amount of an active protein
c. Only translating certain exons
d. Ensuring the ribosome stays bound to the mRNA
19. What are the three types of genes found in bacteria? What are their functions?
20. What is the point of having operons? Draw an example and explain the purpose of the
regulator gene:
21. What are the differences between a positive and negative control in gene regulation?
22. What are the 6 levels at which gene expression can be regulated?
23. Draw out the Nirenberg experiment using polyC’s instead of poly U’s
24. Label the acceptor arm, anticodon arm, DHU arm, TC arm, variable arm, and indicate
what amino acid would be attached
25. What is the wobble hypothesis? What position on the codon does it affect?
26. Draw out the process of translation termination for UAG stop codon, would it look any
different for any other stop codons?
27. List the requirements for each step of translation
Initiation
Elongation
Termination
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