Download Chapter 15

Chapter 15 – Gene
Expression
Control of Gene Expression in
Prokaryotes and Eukaryotes
Sample Pathway for Control of
Promotor (RNATryptophan (trp) Expression
polymerase binding
site)
Enzyme 1
Gene 1
Gene 2
Gene 3
translation
transcription
Enzyme 2
Gene 4
Gene 5
DNA
mRNA
(transcription unit)
Enzyme 3
Enzyme 4
Each enzyme
catalyzes the
next series of
reactions
necessary for
tryptophan
production
Enzyme 5
trp
2 Negative Feedback
shut-off systems
Basic Vocabulary
mRNA Transcription Unit:
- grouping of mRNA genes with related
function
- 2 advantages ~
1. organization
2. easy regulation – one “on/off” switch
can be used to control an entire cluster
of related genes
Basic Vocabulary
Operon:
- an entire gene-regulating system in
PROKARYOTES, which includes the
operator, promotor, and all corresponding
genes
Basic Vocabulary
Operator:
- “on-off” switch to genes
- controls the access of RNA polymerase
to genes
Let’sregion
take a closer
- found within the promotor
or
look at the tryptophan
between the promoter and(trp)gene-encoding
operon…an
example of a
regions
“repressor” system
Overview
Repressor
Trp Operon
Trp
operonofcontrols
the production
of the aminoComponents
acid tryptophan
r. Trp
promotor
Repressor
5 Genes needed for
Gene
tryptophan production
rp. RNA Pol. o. Operator
(always
binding site
“on”)
r.
rp.
o.
Gene 1 Gene 1Gene 3 Gene 4 Gene 5
RNA polymerase
mRNA transcription unit
mRNA for trp
repressor
Enz 1
Trp repressor protein
translated in its
“inactive” form
Enz 2
Enz 3
Enz 4
Enz 5
trp
How is the trp operon turned off
once enough trp is made?
Trp repressor
gene
RNA pol
binding site
operator
trp
trp
trp
trp
Inactive trp
repressor
protein
trp
Increased concentration of trp (corepressor) increases chances of trp
binding to allosteric site of inactive trp
repressor
How is the trp operon turned off
once enough trp is made?
RNA polymerse,
therefore is
physically blocked
from transcribing
genes for trp
Active trp repressor
can now bind to
operator
Why is this a repressor system?
The trp operon is a repressor operon, meaning
gene expression of the operon is repressed
by the presence of the co-repressor,
tryptophan.
Watch this animation to fully
understand and review this operon,
then take the on-line quiz together
as a class
http://bcs.whfreeman.com/thelifewire/content
/chp13/1302002.html
Overview of Inducible Lac Operon
The Lac operon controls the production of
the ß-galactosidase, an enzyme that
catalyzes the hydrolysis (break-down) of
lactose into glucose and galactose.
This is an inducible operon, meaning gene
expression ß-galactosidase is stimulated
by the presence of an co-inducer, lactose.
Inducible Lac operon
promotor
I. Lac
Inducer
I.
mRNA for
inducer
protein
inducer
protein in
“active”
form
crp: helps
RNA pol.
to bind
rp: RNA
pol.binding
site
crp.
rp.
o. operator
Genes that
promote ßgalactosidase
production
o.
RNA pol.
blocked from ßgalactosidase
transcription
without lactose
co-inducer
TWO regulatory mechanisms used
to turn on lac operon
1) Presence of lactose as a co-inducer
** reason?
ß-galactosidase is not needed unless lactose
needs to be broken down
2) Low amounts of glucose
** reason?
Recall that lactose breaks down into glucose and
galactose. Low glucose levels signals the cell
for more lactose to be broken down.
Both conditions must be met for the lac operon to turn on.
TWO regulatory mechanisms used
to turn on lac operon
RNA polymerase can bind
only with the help of CRP
transcription factor
RNA pol.
AlloLactose
cAMP
CRP Transcription
Factor
2. cAMP attaches to CRP
transcription factor when
glucose is scarce
1) Allolactose coinducer attaches to
allosteric site of induce
protein, inactivating it
Lac Operon Animation
Watch this animation to fully understand and
review the lac operon!
http://highered.mcgrawhill.com/olc/dl/120080/bio27.swf
How are genes controlled in
eukaryotes?
1) Regulation of Chromatin Structure
2) Pre and post Transcriptional Regulation
3) Pre and Post Translational Regulation
Regulation of Chromatin Structure
N-terminus (amino group) of histone proteins face outwards
from nucleosome
Tails are thus able to be modified chemically
Regulation of Chromatin Structure
• Methylation  Promotes condensation
• Histone Acetylation – neutralizes (+) charges on tails,
which prevents binding to adjacent nucleosome  loose
chromatin structure results, allowing for increased
transcription
•
Phosphorylation  can prevent condensation, if
phosphorylation is adjacent to methyl group
Pre-Transcriptional Regulation
• Similar to methods used in bacterial
operons, using proteins that inhibit or
promote binding of RNA pol.
– Distal and Proximal Control Elements
– Proteins involved include:
• Transcription factors
• Activators
• Mediator Proteins
Post-Transcriptional Regulation
RNA Processing
– differential/alternative splicing can produce
different 20 mRNA transcript
Differential splicing redefines
which RNA segments are
considered introns and which
are exons
Post-Transcriptional Regulation
• Time of mRNA degradation can vary
Pre-Translational Regulation
Initiation of Translation
- can be blocked by regulatory proteins
that prevent ribosome binding
- shortened polyA tails in mRNA prevents
translation (polyA tails can be added
during appropriate time)
- global regulatory control of all mRNAs in
cell
Post-Translational Regulation
• During protein processing, folding
• Timing of protein degredation can vary
• Proteasomes degrade proteins that are
tagged by ubiquitine molecules