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Download Describe the operon hypothesis and discuss
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Describe the operon hypothesis and discuss how it explains the control of messenger RNA production and the regulation of protein synthesis in bacterial cells. STANDARDS: BACKGROUND: Max. = 2 points __ Definition of operon (functionally related genes whose expression is controlled) __ DNA consists of genes coding for both structural and regulating proteins (Hypothesis) __ Authors of Hypothesis DESCRIPTION OF OPERON (likely the Lac Operon): __ __ __ __ __ __ __ __ __ __ Max. = 8 points Structure (diagram) Max. 4 promoter site repressor site operator site structural genes inducer Function Max. 4 binds RNA polymerase* at 3' site on DNA (* also cAMP-CAP) produces repressor protein: stops RNA polymerase attaching to promoter site of attachment of repressor protein codes for sequential protein serves to inactivate repressor CONTROL OF mRNA PRODUCTION & CONSEQUENCES re PROTEIN SYNTHESIS: __ Inducible model: derepression (lactose example) [gene always off] = 3 points __ Repressible model: corepression (tryptophan-histidine) [gene always on] = 3 points __ CAP model: catabolite induction: with decrease in glucose -> increase in cAMP cAMP-CAP binds to promoter site therefore, transcription -> lactose metabolism = 3 points (above require explanation & example) __ Adaptive significance = 2 points __ Final fate of mRNA transcribed = 2 points Max. = 5