Download BIO 208 TERMS AND OBJECTIVES s08 Objectives Unit 2 Ch 4, 11

BIO 208 TERMS AND OBJECTIVES
Objectives Unit 2 Ch 4, 11, 12, 13
s08
Students will be able to:
1. To compare genomes of mitochondria, H. influenzae, E. coli
2. To describe the E. coli chromosome, size of genome, and nucleoid region
3. To distinguish between a bacterial cell, colony, and lawn
4. To define binary fission
5. To compare bacterial lawns and colonies, solid and liquid cultures
6. To describe stages of bacterial growth - log, lag (exponential growth), stationary, and death
phases
7. To define: prototroph, auxotroph, minimal and complete media
8. To determine bacterial titer OMIT
9. To contrast nutritional, conditional, and resistance mutations in bacteria
10. To discuss the use of nutritional mutants (auxotrophs) in the study of bacterial conjugation
11. To describe parasexual mating (conjugation) between F+ and F- bacteria
12. To explain what the F factor is, what it encodes, and the mechanism of transfer from F+ to
F13. Describe Hfr strains and interrupted mating technique in constructing the E. coli minute map,
predict gene order and plasmid integration orientation based on time for gene transfer
14. To explain why recipient cells of an Hfr mating remain F-.
15. To define homologous recombination in a recipient, exconjugant
16. To examine the E. coli minute map and genomic map
17. To discuss the experiments of Lederberg in the discovery of transduction including the use
of the U-tube and Salmonella/P22 virus system
18. To describe the mechanism of bacteriophage infection
19. To analyze the mechanism of bacterial recombination via faulty head stuffing in transduction
20. To contrast lysogenic and lytic infection, virulent and temperate phages
21. To contrast generalized and specialized transduction OMIT
22. To analyze the use of virally mediated gene therapy and to provide the example of ADA
deficiency
23. To discuss problems in gene therapy
24. To explain the mechanism of transformation and view aspects of plasmids including ori,
ampr, plasmid size, extrachromosomal maintance, and the multiple cloning sites for the
insertion of foreign genes
25. To examine the pGLO plasmid, ori, ampr ,the GFP gene, and the portion of the arabinose
promoter that allows for the regulation of gene expression of GFP by arabinose sugar
26. To transform competent E. coli with a GFP-containing plasmid (lab)
27. To calculate transformation efficiency (colonies/ug DNA) from given data (lab)
28. To contrast constitutively expressed housekeeping genes and genes that are regulated
29. To describe an operon and the usefulness to prokaryotic cells
30. To define the term: polycistronic
31. To understand the regulation of the lac operon by lactose (inducer), repressor, promoter,
RNA polymerase, and the structural genes Z,Y,A, beta galactosidase enzyme, operator.
32. To describe the use of lac operon mutants to elucidate control of operon expression in both
the presence and the absence of lactose
33. To distinguish between cis and trans acting elements in the lac operon
34. To view examples of the use of GFP as a reporter gene
35. To review the steps of gene cloning using a plasmid and bacterium. Including isolation of
DNA from the jellyfish, isolation of the GFP gene using restriction enzymes, ligating the GFP
gene into a plasmid, transformation of E. coli with plasmid.
36. To examine the notion of cell “competency” for transformation
37. To understand that conjugation, tranformation, and transduction are rare events
BIO 208 TERMS AND OBJECTIVES
Objectives Unit 2 Ch 4, 11, 12, 13
Terms Bacterial Genetics (omit terms in smaller font)
antibiotic resistance gene
att site
Bacterial clone, colony, cell, lawn
Bacterial genome
binary fission
colony
colony forming units
conjugation
competent cell
Donor cell, recipient cell
DNAse
E. coli minute map
episome
exponential growth
F factor, F pilus, F+, Ffaulty head stuffing
filterable agent
Hfr
Homologous recombination
Interrupted mating
lag, log, stationary, death phases
lawn
lytic and lysogenic
media – complete, minimal, selective
megabase
mitochondrial genome
minute map
mutant – conditional, nutritional, resistance
nucleoid region
nutritional mutant
open reading frame
origin of replication (ori)
p22 virus
parasexual mating
phage, phage packaging
plasmid
prokaryote
prophage
prototroph and auxotroph
Salmonella typhimurium bacteria
temperate phage and virulent phage
titer determination
transduction
transformation
U-tube
Vector
Viral adsorption, penetration, packaging
s08
BIO 208 TERMS AND OBJECTIVES
Objectives Unit 2 Ch 4, 11, 12, 13
Lac Operon
Beta galactosidase
Cis-acting element
Conformational change
Constitutive mutant
Galactose
Glucose
Housekeeping gene
Inducer
Inducible enzyme
Lac I gene, Lac Z,Y,A structural genes
Lactose
Operator
Operon
Permease
Polymerase
Polycistronic
Promoter
Repressor
Transacetylase
Trans-acting element
Transformation of E. coli with GFP plasmid
Ampicillin resistance gene (bla)
Arabinose inducer
Calcium chloride
Competent bacteria
Cloning vector, multiple cloning site
Fusion protein
Gene cloning
GFP
Heat shock
Jellyfish (bioluminescent)
Ligate
pGLO
Plasmid vector
Origin of replication
Reporter gene
Transformation
s08