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Transcript
Cell Transformation Chapter 13-3
I.
Bacterial Transformation – using a bacteria cell
to produce proteins from other organisms (ex.
Insulin for diabetics)
A. Bacterial DNA forms a round loop called a
Plasmid
B. Genetic Marker- can be a gene for antibiotic
resistance that is spliced onto the gene that
researchers want expressed.
1. Use a restriction enzyme to cut plasmid and
use same enzyme to cut out segment of
DNA
2. This creates ends with nucleotides that
match “sticky ends”
3. Allows bacteria with the gene to be
separated from bacteria that don’t
4. After bacteria cells reproduce the protein,
the protein can be removed and purified for
use
II.
Plant Transformation- A gene can be inserted
into a plant cell using a modified DNA
(bacterial) plasmid and an inactive tumor
producing gene.
III.
Animal Transformation- Using similar
techniques, genes can be inserted into animal
cells.