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Transcript
Cell Transformation Chapter 13-3 I. Bacterial Transformation – using a bacteria cell to produce proteins from other organisms (ex. Insulin for diabetics) A. Bacterial DNA forms a round loop called a Plasmid B. Genetic Marker- can be a gene for antibiotic resistance that is spliced onto the gene that researchers want expressed. 1. Use a restriction enzyme to cut plasmid and use same enzyme to cut out segment of DNA 2. This creates ends with nucleotides that match “sticky ends” 3. Allows bacteria with the gene to be separated from bacteria that don’t 4. After bacteria cells reproduce the protein, the protein can be removed and purified for use II. Plant Transformation- A gene can be inserted into a plant cell using a modified DNA (bacterial) plasmid and an inactive tumor producing gene. III. Animal Transformation- Using similar techniques, genes can be inserted into animal cells.
