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Download BIO 208 TERMS AND OBJECTIVES s08 Objectives Unit 2 Ch 4, 11
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BIO 208 TERMS AND OBJECTIVES Objectives Unit 2 Ch 4, 11, 12, 13 s08 Students will be able to: 1. To compare genomes of mitochondria, H. influenzae, E. coli 2. To describe the E. coli chromosome, size of genome, and nucleoid region 3. To distinguish between a bacterial cell, colony, and lawn 4. To define binary fission 5. To compare bacterial lawns and colonies, solid and liquid cultures 6. To describe stages of bacterial growth - log, lag (exponential growth), stationary, and death phases 7. To define: prototroph, auxotroph, minimal and complete media 8. To determine bacterial titer OMIT 9. To contrast nutritional, conditional, and resistance mutations in bacteria 10. To discuss the use of nutritional mutants (auxotrophs) in the study of bacterial conjugation 11. To describe parasexual mating (conjugation) between F+ and F- bacteria 12. To explain what the F factor is, what it encodes, and the mechanism of transfer from F+ to F13. Describe Hfr strains and interrupted mating technique in constructing the E. coli minute map, predict gene order and plasmid integration orientation based on time for gene transfer 14. To explain why recipient cells of an Hfr mating remain F-. 15. To define homologous recombination in a recipient, exconjugant 16. To examine the E. coli minute map and genomic map 17. To discuss the experiments of Lederberg in the discovery of transduction including the use of the U-tube and Salmonella/P22 virus system 18. To describe the mechanism of bacteriophage infection 19. To analyze the mechanism of bacterial recombination via faulty head stuffing in transduction 20. To contrast lysogenic and lytic infection, virulent and temperate phages 21. To contrast generalized and specialized transduction OMIT 22. To analyze the use of virally mediated gene therapy and to provide the example of ADA deficiency 23. To discuss problems in gene therapy 24. To explain the mechanism of transformation and view aspects of plasmids including ori, ampr, plasmid size, extrachromosomal maintance, and the multiple cloning sites for the insertion of foreign genes 25. To examine the pGLO plasmid, ori, ampr ,the GFP gene, and the portion of the arabinose promoter that allows for the regulation of gene expression of GFP by arabinose sugar 26. To transform competent E. coli with a GFP-containing plasmid (lab) 27. To calculate transformation efficiency (colonies/ug DNA) from given data (lab) 28. To contrast constitutively expressed housekeeping genes and genes that are regulated 29. To describe an operon and the usefulness to prokaryotic cells 30. To define the term: polycistronic 31. To understand the regulation of the lac operon by lactose (inducer), repressor, promoter, RNA polymerase, and the structural genes Z,Y,A, beta galactosidase enzyme, operator. 32. To describe the use of lac operon mutants to elucidate control of operon expression in both the presence and the absence of lactose 33. To distinguish between cis and trans acting elements in the lac operon 34. To view examples of the use of GFP as a reporter gene 35. To review the steps of gene cloning using a plasmid and bacterium. Including isolation of DNA from the jellyfish, isolation of the GFP gene using restriction enzymes, ligating the GFP gene into a plasmid, transformation of E. coli with plasmid. 36. To examine the notion of cell “competency” for transformation 37. To understand that conjugation, tranformation, and transduction are rare events BIO 208 TERMS AND OBJECTIVES Objectives Unit 2 Ch 4, 11, 12, 13 Terms Bacterial Genetics (omit terms in smaller font) antibiotic resistance gene att site Bacterial clone, colony, cell, lawn Bacterial genome binary fission colony colony forming units conjugation competent cell Donor cell, recipient cell DNAse E. coli minute map episome exponential growth F factor, F pilus, F+, Ffaulty head stuffing filterable agent Hfr Homologous recombination Interrupted mating lag, log, stationary, death phases lawn lytic and lysogenic media – complete, minimal, selective megabase mitochondrial genome minute map mutant – conditional, nutritional, resistance nucleoid region nutritional mutant open reading frame origin of replication (ori) p22 virus parasexual mating phage, phage packaging plasmid prokaryote prophage prototroph and auxotroph Salmonella typhimurium bacteria temperate phage and virulent phage titer determination transduction transformation U-tube Vector Viral adsorption, penetration, packaging s08 BIO 208 TERMS AND OBJECTIVES Objectives Unit 2 Ch 4, 11, 12, 13 Lac Operon Beta galactosidase Cis-acting element Conformational change Constitutive mutant Galactose Glucose Housekeeping gene Inducer Inducible enzyme Lac I gene, Lac Z,Y,A structural genes Lactose Operator Operon Permease Polymerase Polycistronic Promoter Repressor Transacetylase Trans-acting element Transformation of E. coli with GFP plasmid Ampicillin resistance gene (bla) Arabinose inducer Calcium chloride Competent bacteria Cloning vector, multiple cloning site Fusion protein Gene cloning GFP Heat shock Jellyfish (bioluminescent) Ligate pGLO Plasmid vector Origin of replication Reporter gene Transformation s08