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Transcript
TASKS
• Map a cloned rice blast
resistance gene to its
putative location in the rice
genome
• Compare its position to that
of other mapped resistance
genes
What do we already know ?
• The rice disease resistance
gene Pi-ta
• Genetically mapped to
chromosome 12 Rybka et al. (1997).
• It has also been sequenced
Bryan et al. (1997).
Gramene Literature
View Gramene record for Pita gene from rice
Trait Ontology Browser
View Gramene record for Pita gene from rice
Pita gene product record
Gramene Blast Search
Copy sequence
BLAST Search Results
Ensembl protein report
Ensembl Protein Report page, which provides information
about the identity, description, and structure of the protein.
Ensembl Gene Report
Gene Model id
TIGR gene Id
Location of Pi-ta gene
on the rice genome
Ensembl Gene Report
page provides information
about the identity, base pair
location on the
chromosome, description,
and structure.
Genome Browser Overview
Red window on
chromosome
indicates region
shown in
“Overview”.
Red window on
“Overview” indicates
region shown in
“Detailed View”.
Nearest rice
marker.
Genome Browser Detailed View
Choose additional
tracks from the
popup menu.
Transcription units
from TIGR gene
models.
Pi-ta Gene Neighborhood
Genes mapped
to + strand.
Genes mapped
to − strand.
Putative Pi-ta gene
models
Click to get the
popup menu. Click
“Gramene Marker”
to go to marker
database.
Marker Database
Click to view
marker details.
Click to view
marker on map.
CMap Comparative Map Viewer
Map Set Info
CMap – Add comparative maps
Blast
resistance
genes
Blast
resistance
QTL
CMap Options
CMap Feature Details
QTL Search
QTL Details