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Genome Browsers – Tutorial Carsten O. Daub Omics Science Center, RIKEN, Japan May 2008 Task1 • Go to the human p53 gene • Where is it located? • How many splice forms do you find – According to the UCSC gene controls – According to ENSEMBL gene controls • Which ENSEMBL transcript corresponds to the RefSeq gene? • How does sequence conservation correspond to the exon structure? • Which RefSeq genes are upstream and downstream of p53 on the same strand? What is roughly the distance? • Do a similar search using UCSC and ENSEMBL! Which one is easier to use? Task 2 • • • • • Go to your favourite gene Customize the tracks according to your interest Make a picture in the PDF format Are there any miRNAs targeting your gene? Add the following PicTar miRNA prediction track and check again – http://pictar.bio.nyu.edu/new_mammals_bed • Are there any PicTar targets for the human HIC2 genes? Where are they located in the gene?