Download Chapter_10_HB_Molecular_Biology

Chapter 10
Molecular Biology of the
Gene
PowerPoint Lectures for
Biology: Concepts and Connections, Fifth Edition
– Campbell, Reece, Taylor, and Simon
Lectures by Chris Romero
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Sabotage Inside Our Cells
•
Viruses are biological saboteurs
– Hijack the genetic material of host cells in order to
reproduce themselves
– May remain permanently dormant in the body
•
Viruses share some characteristics of living organisms
but are not generally considered alive
– Genetic material composed of nucleic acid
– Not cellular
– Cannot reproduce on their own
•
First understanding of DNA based on viruses
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
THE STRUCTURE OF THE GENETIC MATERIAL
10.1 Experiments showed that DNA is the
genetic material
• Hershey-Chase experiments in 1952
determined that the heredity material was DNA
not protein
– Studied the simple bacteriophage T2
– Showed that the virus injects its DNA into
host cells and reprograms them to produce
more viruses
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 10-1a
Head
DNA
Tail
300,000
Tail fiber
LE 10-1b
Phage
Radioactive
protein
Bacterium
DNA
Batch 1
Radioactive
protein
Mix radioactively
labeled phages with
bacteria. The phages
infect the bacterial cells.
Batch 2
Radioactive
DNA
Empty
protein shell
Radioactivity
in liquid
Phage
DNA
Centrifuge
Agitate in a blender to
separate phages outside
the bacteria from the
cells and their contents.
Pellet
Centrifuge the mixture Measure the
so bacteria form a
radioactivity in
pellet at the bottom of
the pellet and
the test tube.
the liquid.
Radioactive
DNA
Centrifuge
Pellet
Radioactivity
in pellet
LE 10-1c
Phage attaches
to bacterial cell.
Phage injects DNA.
Phage DNA directs host
cell to make more phage
DNA and protein parts.
New phages assemble.
Cell lyses and
releases
new phages.
10.2 DNA and RNA are polymers of nucleotides
• Nucleic acids are polynucleotides made of long
chains of nucleotide monomers
Single-ring pyrimidines: thymine (T), cytosine ( C)
Double-ring purines: adenine (A), guanine (G)
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 10-2c
Nitrogenous base
(A, G, C, or U)
Phosphate
group
Uracil (U)
Sugar
(ribose)
LE 10-2b
Cytosine (C)
Thymine (T)
Pyrimidines
Adenine (A)
Guanine (G)
Purines
LE 10-2a
Sugar-phosphate backbone
Phosphate group
A
Nitrogenous base
Sugar
A
C
C
DNA nucleotide
Nitrogenous base
(A, G, C, or T)
Phosphate
group
T
T
Thymine (T)
G
G
Sugar
(deoxyribose)
T
T
DNA nucleotide
DNA polynucleotide
• DNA and RNA are identical except for two
things
– Nitrogenous bases
• DNA: A, C, G, T
• RNA: A, G, C, U
– Sugars
• DNA: Deoxyribose
• RNA: Ribose
Animation: DNA and RNA Structure
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 10-2d
Key
Hydrogen atom
Carbon atom
Nitrogen atom
Oxygen atom
Phosphorus atom
10.3 DNA is a double-stranded helix
• James Watson and Francis Crick worked out
the three-dimensional structure of DNA, based
on X-ray crystallography by Rosalind Franklin
& Maurice Wilkins
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
• DNA consists of two polynucleotide strands
wrapped around each other in a double helix
– Sugar-phosphate backbones are on the
outside and nitrogenous bases on the inside
– Each base pairs with a complementary
partner
• A with T, and G with C
– Hydrogen bonds between the bases hold
the strands together
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 10-3c
Twist
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 10-3d
C
G
T
A
T
A
Base
pair
C
Hydrogen bond
T
G
C
G
A
T
A
C
G
C
G
T
T
C
A
G
A
A
T
A
T
A
G
A
Ribbon model
T
C
T
Partial chemical structure
Computer model
DNA REPLICATION
10.4 DNA replication depends on specific base pairing
• Replication process
– DNA strands separate
– Enzymes use each strand as a template to
assemble new nucleotides into complementary
strands
• The mechanism of DNA replication is
semiconservative
– Each new double helix consists of one old and
one new strand
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 10-4a
A
T
A
T
C
G
C
G
G
C
G
A
T
A
T
A
T
Parental
molecule
of DNA
A
C
C
Nucleotides
Both parental
strands serve
as templates
T
A
T
A
T
G
C
G
C
G
C
G
C
G
C
T
A
T
A
T
A
T
A
T
A
Two identical
daughter molecules
of DNA
LE 10-4b
G C
A T
G
C
C
G
A T
C
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
10.5 DNA replication: A closer look
• DNA replication begins at specific sites (origins
of replication) on the double helix
– Proteins (helicase) attach and break the
hydrogen bonds separating the strands
– Replication proceeds in both directions,
creating replication bubbles
– A second strand of new DNA is synthesized
along each separated strand by DNA
polymerases, which position free
nucleotides across from complementary
nucleotides
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DNA Replication
• Parent strands open, daughter strands
elongate
– Replication occurs simultaneously at many
sites
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LE 10-5a
Origin of replication
Parental strand
Daughter strand
Bubble
Two daughter DNA molecules
• DNA's sugar-phosphate backbones are
oriented in opposite directions (anti-parallel)
– The enzyme DNA polymerase adds
nucleotides at only the 3’ end
• One daughter strand is synthesized as a
continuous piece
• The other strand is synthesized as a series
of short pieces (Okazaki Fragments)
• The two strands are connected by the
enzyme DNA ligase
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 10-5b
3 end
5 end
P
HO
5
2
4
3
1
A
T
2
P
C
G
P
P
G
C
P
P
T
3 end
4
5
P
OH
3
1
A
P
5 end
LE 10-5c
DNA polymerase
molecule
3
5
5
Daughter strand
synthesized
continuously
Parental DNA
3
3
5
5
3
DNA ligase
Overall direction of replication
Daughter
strand
synthesized
In pieces
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
THE FLOW OF GENETIC INFORMATION
FROM DNA TO RNA TO PROTEIN
10.6 The DNA genotype is expressed as
proteins, which provide the molecular basis for
phenotypic traits
• The information constituting an organism's
genotype is carried in its sequence of DNA
bases
• A particular gene—a linear sequence of many
nucleotides—specifies a particular polypeptide
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
How Did Scientists Discover
That Genes Are Made of DNA?
– Transformed Bacteria Revealed the Link
Between Genes and DNA
Genes Are Made of DNA
•
Known since the late 1800s:
1. Heritable information is carried in discrete
units called genes
2. Genes are parts of structures called
chromosomes
3. Chromosomes are made of
deoxyribonucleic acid (DNA) and protein
Genes Are Made of DNA
• Transformed bacteria revealed the link
between genes and DNA
Genes Are Made of DNA
• F. Griffith worked with two strains of
Streptococcus pneumoniae bacteria
– S strain caused pneumonia when injected into
mice, killing them
– R strain did not cause pneumonia when injected
Genes Are Made of DNA
• Griffith made a sample of heat-killed S strain
and mixed it with R strain
– Injection of combination into mice caused
pneumonia and death
Genes Are Made of DNA
• Deductions from Griffith’s experiment (1920s)
– Living safe bacteria (R strain) were changed by
something in the dead (but normally diseasecausing) S strain
– The living R strain bacteria were transformed
by genetic material released by the S strain
Genes Are Made of DNA
• Later findings by Avery, MacLeod, and
McCarty (1940s)
– The transforming molecule from the S strain
was DNA
The Link Between DNA and Protein
•
•
DNA contains the molecular blueprint of
every cell
Proteins are the “molecular workers” of the
cell
The Link Between DNA and Protein
•
•
Proteins control cell shape, function,
reproduction, and synthesis of
biomolecules
The information in DNA genes must
therefore be linked to the proteins that run
the cell
One Gene Encodes One Protein
• Synthesis of new molecules inside the cell
occurs through biochemical pathways
• Each step in a biochemical pathway is
catalyzed by a protein enzyme
One Gene Encodes One Protein
• George Beadle and Edward Tatum showed
that one DNA gene encodes the information
for one enzyme (protein) in a biochemical
pathway
• Studies of inherited
metabolic disorders
in mold suggested
that phenotype is expressed
through proteins
• The hypothesis has been restated to
one gene-one polypeptide
RNA Intermediaries
• DNA holds the information on how to make
proteins
• DNA in eukaryotes is kept in the nucleus; it
can’t leave
• Protein synthesis occurs at ribosomes in the
cytoplasm
• How do instructions on DNA get to the
ribosomes outside of the nucleus?
RNA Intermediaries
• DNA information must be carried by an
intermediary (RNA) from nucleus to
cytoplasm
RNA Intermediaries
• RNA differs structurally from DNA
– RNA is single stranded
– RNA uses the sugar ribose
– RNA uses the nitrogenous base uracil (U)
instead of thymine (T)
RNA Intermediaries
• There are three types of RNA involved in
protein synthesis
– Messenger RNA (mRNA) carries DNA gene
information to the ribosome
– Transfer RNA (tRNA) brings amino acids to
the ribosome
– Ribosomal RNA (rRNA) is part of the structure
of ribosomes
Transcription and Translation
•
DNA directs protein synthesis in a two-step
process
1. Information in a DNA gene is copied into
mRNA in the process of transcription
2. mRNA, together with tRNA, amino acids, and
a ribosome, synthesize a protein in the
process of translation
10.7 Genetic information written in codons is
translated into amino acid sequences
• Genetic information flows from
DNA
RNA
protein
• Nucleotide monomers represent letters in an
alphabet that can form words in a language
– Triplet code
• Three-letter words (codons)
• Each word codes for one amino acid in a
polypeptide
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 10-7a
DNA molecule
Gene 1
Gene 2
Gene 3
DNA strand
A
A
A
C
C
G
G
C
A
A
A
A
U
U
U
G
G
C
C
G
U
U
U
U
Transcription
RNA
Codon
Translation
Polypeptide
Amino acid
10.8 The genetic code is the Rosetta stone of life
• The genetic code specifies the
correspondence between RNA codons and
amino acids in proteins
– Includes start (AUG-methionine) and 3 stop
codons
– Redundant but not ambiguous
• Nearly all organisms use exactly the same
genetic code
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 10-8a
Second base
C
U
A
UAU
UCU
UUU
Phe
U
UGU
Cys
Tyr
UAC
UCC
UUC
G
C
UGC
Ser
U
UUA
UCA
UAA
Stop
UGA
Stop
A
UUG
UCG
UAG
Stop
UGG
Trp
G
CUU
CCU
CAU
CUC
CCC
CAC
Leu
U
CGU
His
Leu
C
CUA
CGU
Pro
CCA
C
Arg
CAA
CGA
A
CGG
G
Gln
CUG
CCG
CAG
AUU
ACU
AAU
AUC
lle
ACC
U
AGU
Ser
Asn
AAC
AGC
C
ACA
AAA
AGA
A
ACG
AAG
AGG
G
GCU
GAU
GGU
U
Thr
A
AUA
AUG
Met or
start
GUU
Arg
Lys
Asp
GUC
G
GCC
Val
GUA
GAC
GCA
C
GGC
Gly
Ala
GAA
GGA
A
GGG
G
Glu
GUG
GCG
GAG
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 10-8b
Strand to be transcribed
T
A
C
T
T
C
A
A
A
A
T
C
A
T
G
A
A
G
T
T
T
T
A
G
U
A
G
DNA
Transcription
A
U
G
A
A
G
U
U
U
RNA
Stop
codon
Start
codon
Translation
Polypeptide
Met
Lys
Phe
10.9 Transcription produces genetic messages in
the form of RNA
• One DNA strand serves as a template for the
new RNA strand
• RNA polymerase constructs the RNA strand in
a multistep process
– Initiation:
• RNA polymerase attaches to the promotor
• Synthesis starts
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• Elongation:
– RNA synthesis continues
– RNA strand peels away from DNA template
– DNA strands come back together in
transcribed region
• Termination:
– RNA polymerase reaches a terminator
sequence at the end of the gene
– Polymerase detaches
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 10-9a
RNA nucleotides
RNA
polymerase
C
C
A
A
U
C
C
A
T
A
G
G
T
Direction of
transcription
Newly made RNA
A
T
Template
strand of DNA
LE 10-9b
RNA polymerase
DNA of gene
Promoter
DNA
Terminator
DNA
Initiation
Elongation
Termination
Completed RNA
Area shown
In Figure 10.9A
Growing
RNA
RNA
polymerase
10.10 Eukaryotic RNA is processed before
leaving the nucleus
• The RNA that encodes an amino acid
sequence is messenger RNA (mRNA)
• In prokaryotes, transcription and translation
both occur in the cytoplasm
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
• In eukaryotes, RNA transcribed in the nucleus
is processed before moving to the cytoplasm
for translation
• RNA Splicing
– Noncoding segments called introns are cut
out
– Remaining exons are joined to form a
continuous coding sequence
– A cap and a tail are added to the ends
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 10-10
Exon Intron
Exon
Intron
Exon
DNA
Cap
RNA
transcript
with cap
and tail
Transcription
Addition of cap and tail
Introns removed
Tail
Exons spliced together
mRNA
Coding sequence
Nucleus
Cytoplasm
10.11 Transfer RNA molecules serve as interpreters
during translation
•
Transfer RNA (tRNA) molecules match the right amino
acid to the correct codon
•
tRNA is a twisted and folded single strand of RNA
– Anticodon loop at one end recognizes a particular
mRNA codon by base pairing
– Amino acid attachment site is at the other end
•
Each amino acid is joined to the correct tRNA by a
specific enzyme
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 10-11a
Amino acid attachment site
Hydrogen bond
RNA polynucleotide chain
Anticodon
LE 10-11b
Amino acid
attachment site
Anticodon
10.12 Ribosomes build polypeptides
• A ribosome consists of two subunits
– Each is made up of proteins and ribosomal
RNA (rRNA)
• The subunits of a ribosome
– Hold the tRNA and mRNA close together in
binding sites during translation
– Allow amino acids to be connected into a
polypeptide chain
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 10-12a
tRNA
molecules
Growing
polypeptide
Large
subunit
mRNA
Small
subunit
LE 10-12b
tRNA-binding sites
Large
subunit
mRNA
binding
site
Small
subunit
LE 10-12c
Next amino acid
to be added to
polypeptide
Growing
polypeptide
tRNA
mRNA
Codons
10.13 An initiation codon marks the start of an
mRNA message
• The initiation phase of translation
– Brings together mRNA, a specific tRNA,
and the two subunits of a ribosome
– Establishes exactly where translation will
begin
• Ensures that mRNA codes are translated in
the correct sequence
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
• Initiation is a two-step process
– Step 1
• mRNA binds to a small ribosomal subunit
• Initiator tRNA, carrying the amino acid Met,
binds to the start codon
– Step 2
• A large ribosomal subunit binds to the small
one, forming a functional ribosome
• Initiator tRNA fits into one binding site; the
other is vacant for the next tRNA
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 10-13a
Start of genetic message
End
LE 10-13b
Large
Ribosomal
subunit
Initiator tRNA
P site
U A C
A U G
U A C
A UG
Start codon
mRNA
A site
Small ribosomal
subunit
10.14 Elongation adds amino acids to the
polypeptide chain until a stop codon terminates
translation
•
Once initiation is complete, amino acids are
added one by one in a three-step elongation
process
1. Codon recognition
2. Peptide bond formation
3. Translocation
•
Elongation continues until a stop codon reaches
the ribosome's A site, terminating translation
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 10-14
Amino
acid
Polypeptide
A site
P site
Anticodon
mRNA
Codons
Codon recognition
mRNA
movement
Stop
codon
Peptide bond
formation
New
peptide
bond
Translocation
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 10-15
Transcription
DNA
mRNA
RNA
polymerase
mRNA is
transcribed from a
DNA template.
Translation
Amino acid
Enzyme
Each amino acid
attaches to its proper
tRNA with the help of a
specific enzyme and ATP.
ATP
tRNA
Anticodon
Initiator
tRNA
U AC
AU G
Start Codon
mRNA
Large
Initiation of
ribosomal
polypeptide synthesis
subunit
The mRNA, the first
tRNA, and the ribosomal
Sub units come together.
Small
ribosomal
subunit
New peptide
bond forming
Growing
polypeptide
Codons
mRNA
Elongation
A succession of tRNAs
add their amino acids to
the polypeptide chain
as the mRNA is moved
through the ribosome,
one codon at a time.
Polypeptide
Termination
Stop codon
The ribosome recognizes
a stop codon. The polypeptide is terminated
and released.
10.15 Review: The flow of genetic information in
the cell is DNA  RNA  protein
•
The sequence of codons in DNA, via the
sequence of codons in RNA, spells out the
primary structure of a polypeptide
1. Transcription of mRNA from a DNA
template
2. Attachment of amino acid to tRNA
3. Initiation of polypeptide synthesis
4. Elongation
5. Termination
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
10.16 Mutations can change the meaning of
genes
• Mutation: any change in the nucleotide
sequence of DNA
– Caused by errors in DNA replication or
recombination, or by mutagens
– Can involve large regions of a chromosome
or a single base pair
– Can cause many genetic diseases, such as
sickle-cell disease
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Mutations Fuel Evolution
• Mutations are heritable changes in the DNA
• Approx. 1 in 105-106 eggs or sperm carry a
mutation
• Most mutations are harmful or neutral
Mutations Fuel Evolution
• Mutations create new gene sequences and
are the ultimate source of genetic variation
• Mutant gene sequences that are beneficial
may spread through a population and
become common
LE 10-16a
Normal hemoglobin DNA
C
T
Mutant hemoglobin DNA
T
mRNA
C
A
T
G
U
A
mRNA
G
A
A
Normal hemoglobin
Sickle-cell hemoglobin
Glu
Val
• Two general categories of genetic mutations
– Base substitutions replace one base with
another
• Most are harmful but may occasionally have
no effect or be beneficial
– Base insertions or deletions alter the
reading frame
• Result is most likely a nonfunctioning
polypeptide
• Mutagenesis caused by spontaneous error or
a physical or chemical mutagen
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 10-16b
Normal gene
A
U
G
A
A
G
U
U
U
G
G
C
G
C
A
mRNA
Protein
Met
Lys
Phe
Gly
Ala
Base substitution
A
U
G
A
Met
A
G
U
Lys
G
A
Phe
Base deletion
A
U
U
C
G
Ser
C
A
Ala
U Missing
U
Met
G
A
A
Lys
G
U
U
Leu
G
G
C
Ala
G
C
A
His
U
MICROBIAL GENETICS
10.17 Viral DNA may become part of the host
chromosome
• Viruses are infectious particles consisting of
nucleic acid enclosed in a protein capsid
• Viruses depend on their host cells for the
replication, transcription, and translation of
their nucleic acid
– DNA enters host bacterium, circularizes,
and enters one of two pathways
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– Lytic cycle
• Host produces more viruses
• Host cell lyses (breaks open) to release new
viruses
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– Lysogenic cycle
• Phage DNA inserted by recombination into
the host chromosome; is now a prophage
• Prophages replicated each time host cell
divides; passed on to generations of
daughter cells
• Does not destroy host
• Environmental signal may trigger switch
from lysogenic to lytic cycle
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 10-17
Phage
Attaches
to cell
Phage DNA
Cell lyses,
releasing phages
Bacterial
chromosome
Phage injects DNA
Many cell
divisions
Lytic cycle
Phages assemble
Lysogenic cycle
Phage DNA
circularizes
Prophage
Lysogenic bacterium reproduces normally, replicating the
prophage at each cell division
OR
New phage DNA and
proteins are synthesized
Phage DNA inserts into the bacterial
chromosome by recombination
CONNECTION
10.18 Many viruses cause disease in animals
• Structure of a virus that invades animal cells
– Genetic material may be RNA (examples:
flu, HIV) or DNA (examples: hepatitis,
herpes)
– Protein coat
– Sometimes a membranous envelope with
glycoprotein spikes
• The envelope helps the virus enter and leave
the host cell during its reproductive cycle
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 10-18a
Membranous
envelope
RNA
Protein
coat
Glycoprotein spike
LE 10-18b
VIRUS
Viral RNA
(genome)
Plasma membrane
of host cell
Glycoprotein spike
Protein coat
Envelope
Entry
Uncoating
Viral RNA
(genome)
RNA synthesis
by viral enzyme
Protein
synthesis
RNA synthesis
(other strand)
mRNA
Template
New viral
genome
New
viral proteins
Assembly
Exit
CONNECTION
10.19 Plant viruses are serious agricultural pests
• Most plant viruses
– Have RNA genomes
– Enter their hosts via wounds in the plant's
outer layers
– May spread throughout the plant through
plasmodesmata
• There is no cure for most plant viruses
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 10-19
Protein
RNA
CONNECTION
10.20 Emerging viruses threaten human health
• Emerging viruses have appeared suddenly or
have recently come to the attention of
scientists
– Examples: HIV, SARS, Ebola, West Nile
• Processes contributing to emergence
– Mutation
– Contact between species
– Spread from isolated populations
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
10.21 The AIDS virus makes DNA on an RNA
template
• HIV, the AIDS virus, is a retrovirus
– Flow of genetic information is RNA _ DNA
– Inside a cell, HIV uses its RNA as a
template for making DNA
– The enzyme reverse transcriptase catalyzes
reverse transcription
Animation: HIV Reproductive Cycle
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 10-21a
Envelope
Glycoprotein
Protein
coat
RNA
(two identical
strands)
Reverse
transcriptase
LE 10-21b
Viral RNA
CYTOPLASM
NUCLEUS
DNA
strand
Chromosomal
DNA
Doublestranded
DNA
Viral
RNA
and
proteins
Provirus
DNA
RNA
10.22 Bacteria can transfer DNA in three ways
• Bacteria can transfer genes from cell to cell by
one of three processes
– Transformation: the uptake of foreign DNA
from the surrounding environment
– Transduction: transfer of bacterial genes by
a phage
– Conjugation: union of two bacterial cells and
the transfer of DNA between them
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 10-22a
DNA enters
cell
Fragment of
DNA from
another
bacterial cell
Bacterial chromosome
(DNA)
LE 10-22b
Phage
Fragment of DNA from
another bacterial cell
(former phage host)
LE 10-22c
Mating bridge
Sex pili
Donor cell
(“male”)
Recipient cell
(“female”)
• Once new DNA is in a bacterial cell, part of it
may integrate into the recipient's chromosome
– Occurs by crossing over between the two
molecules
– Leaves the recipient with a recombinant
chromosome
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 10-22d
Donated DNA
Recipient cell’s
chromosome
Crossovers
Degraded DNA
Recombinant
chromosome
10.23 Bacterial plasmids can serve as carriers for
gene transfer
• The F factor is a piece of bacterial DNA
– Carries genes for things needed for
conjugation
– Contains an origin of replication
– Can transfer chromosomal DNA by
integrating into the donor bacterium's
chromosome or entering the cell as a
plasmid
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 10-23a
F factor (integrated)
Male (donor)
cell
Origin of F
replication
Bacterial
chromosome
F factor starts replication
and transfer of chromosome
Recipient cell
Only part of the
chromosome transfers
Recombination can occur
LE 10-23b
F factor (plasmid)
Male (donor)
cell
Bacterial
chromosome
F factor starts replication
and transfer
Plasmid completes
transfer and circularizes
Cell now male
• Plasmids
– Small circular DNA molecules separate
from the bacterial chromosome
– Can serve as carriers for the transfer of
genes
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Colorized TEM 2,000
LE 10-23c
Plasmids