* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download New Microsoft Office PowerPoint Presentation
Epigenetics of human development wikipedia , lookup
Saethre–Chotzen syndrome wikipedia , lookup
Copy-number variation wikipedia , lookup
Human genome wikipedia , lookup
Genetic engineering wikipedia , lookup
Point mutation wikipedia , lookup
Epigenetics of neurodegenerative diseases wikipedia , lookup
History of genetic engineering wikipedia , lookup
No-SCAR (Scarless Cas9 Assisted Recombineering) Genome Editing wikipedia , lookup
Genomic imprinting wikipedia , lookup
Long non-coding RNA wikipedia , lookup
Vectors in gene therapy wikipedia , lookup
Transposable element wikipedia , lookup
Neuronal ceroid lipofuscinosis wikipedia , lookup
Epigenetics of diabetes Type 2 wikipedia , lookup
Genome (book) wikipedia , lookup
Metagenomics wikipedia , lookup
Epitranscriptome wikipedia , lookup
Pathogenomics wikipedia , lookup
Public health genomics wikipedia , lookup
Gene therapy wikipedia , lookup
Genome evolution wikipedia , lookup
Primary transcript wikipedia , lookup
Nutriepigenomics wikipedia , lookup
Gene nomenclature wikipedia , lookup
Gene desert wikipedia , lookup
Gene therapy of the human retina wikipedia , lookup
Gene expression programming wikipedia , lookup
Microevolution wikipedia , lookup
Site-specific recombinase technology wikipedia , lookup
Gene expression profiling wikipedia , lookup
Therapeutic gene modulation wikipedia , lookup
Designer baby wikipedia , lookup
Genome editing wikipedia , lookup
Helitron (biology) wikipedia , lookup
Introduction to Bioinformatics II Lecture 12 Expressed Sequence Tags By Ms. Shumaila Azam Expressed Sequence Tags (ESTs) • Unedited, short, single pass sequences generated from 5' or 3' end of randomly selected cDNA libraries in desired cells/tissues/organ. • Length: 200-700 bp (average 360 bp) • Can be quickly generated at low cost (“poorman’s genome”) • EST annotations have very little biological information EST… • An expressed sequence tag or EST is a short sub-sequence of a cDNA sequence. • They may be used – to identify gene transcripts, – and are instrumental in gene discovery – and gene sequence determination. • 74.2 million ESTs now available in public databases • the ESTs represent portions of expressed genes. • cDNA/mRNA sequence or as the reverse complement of the mRNA, the template strand. EST data repositories dbEST release 061507 (June, 2007) www.ncbi.nlm.nih.gov/dbEST/ 43,396,096 ESTs from 659 different organisms Homo sapiens (human) 8,119,106 Mus musculus (mouse) 4,850,243 Danio rerio (zebrafish) 1,350,105 Bos taurus (cattle) 1,318,208 Arabidopsis thaliana (thale cress) 1,276,692 Xenopus tropicalis 1,271,375 Oryza sativa (rice) 1,211,418 Zea mays (maize) 1,161,241 Triticum aestivum (wheat) 1,050,267 EST Applications Gene Discovery Gene Structure Prediction Expression Maps Alternative Splicing Identification and characterization of SNPs Gene expression studies tissue or disease specific developmental stage Proteomics (for example peptide mass fingerprinting) Identification of drug and vaccine candidates