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I. The Cell- The basic unit of structure and function in all living things. Cells are open systems that require an exchange of materials with their surroundings. Evolution is the basis for the correlation of structure and function A. How Cells are Studied 1. Light Microscope- light is refracted through lenses, magnifying the image. Resolution is limited to by the wavelength of visible light. Effective magnification is about 1500 X a) Magnification- how large an image appears b) Resolution- measure of the clarity of the image 2. Electron Microscope- focuses a beam of electrons through the specimen. Resolution is inversely related to the wavelength of radiation a microscope uses. Best resolution is about 0.2nm a) TEM- transmission electron microscope shoots beams of electrons through a specimen. Used to view internal structure b) SEM- scanning electron microscope shoots electrons off the surface of objects that have been coated in gold. Used to study surfaces of specimen Which is the SEM and which is the TEM? 3. Cell Fractionation- technique used to study cell parts called organelles a) Cells are homogenized by disrupting the cell membrane b) The remaining liquid is placed in a centrifuge- a spinning device which will separate materials according to size B. Types of Cells: Prokaryotic Vs. Eukaryotic Cells 1. Prokaryotic Cells • small cells with no true nucleus and membrane bound organelles. • Examples: Bacteria and Blue-green algae 2. Eukaryotic Cells- Cells that have a nucleus and membrane bound organelles. Plant Animal Fungus Protist C. Cell Size 1. Cells have a wide range of sized A) Mycoplasma: 0.1-1.0 μm B) Most bacteria: 1 to 10 μm C) Most Eukaryotic cells: 10 to 100 μm 2. Cell size limitations A) Based on the ability of the cell to obtain oxygen, nutrients and to get rid of wastes. B) Surface area to volume ratios are extremely important Surface Area Vs. Volume Surface Area (Cell membrane)- place where all oxygen, food and wastes must enter and exit the cell Volume (cytoplasm)substance that uses up food, oxygen and produces waste Sammy Cell Surface area = length X width X number of sides Surface area = 1 μm X 1 μm X 6 1 μm 1 μm Surface area = 6 μm2 1 μm Volume = length X width X height Volume = 1 μm X 1 μm X 1 μm Volume = 1 μm3 Surface Area to Volume Ratio = 6 : 1 Cell Double Size Surface area = 2 μm X 2 μm X 6 2 μm 2 μm 2 μm Surface area = 24 μm2 Volume = 2 μm X 2 μm X 2 μm Volume = 8 μm3 Surface Area to Volume Ratio = 3 : 1 Cell Triples Size Surface area = 3 μm X 3 μm X 6 3 μm Surface area = 54 μm2 Volume = 3 μm X 3 μm X 3 μm 3 μm 3 μm Volume = 27 μm3 Surface Area to Volume Ratio = 2 : 1 3. As a cell grows, its need for oxygen increases faster than its ability to get oxygen. So the cell must divide or suffocate 3. The Importance of Compartmental organization A) Eukaryotic Cells are surrounded by a plasma membrane and are partitioned into organelles which provide correct environments for specific metabolic processes B) Many enzymes are built into the membranes E. Organelles of the Eukaryotic Cell 1. Nucleus- organelle responsible for the control of all cellular processes A) A nuclear envelope determines what materials enter and exit the nucleus through the nuclear pores B) In the nucleoplasm are a set number of chromosomes composed of tightly coiled strands of DNA C) Segments of DNA that are responsible for the production of a protein are called genes, which produce m-RNA D) While the cell is not dividing, loose strands of DNA appear grainy and are called chromatin E) Nucleolus- dark spherical structure in the nucleus that is rich in RNA and is responsible for the production of ribosomes Nuclear membrane Nuclear pore Chromatin Nucleolus Chromosomes Chromosome Vs Chromatin DNA Packing 2. Endoplasmic Reticulum- network of internal membranes extending through the cell a) The ER transports materials in the cell b) Cisternae, flattened sacs where the ER has expanded, may serve as a storage area for important substances c) lipids, proteins and complex carbohydrates are also synthesized in the ER d) Smooth ER (SER) produces steroids, stores calcium and detoxifies certain poisons e) Rough ER (RER) ER with ribosomes 3. Ribosomes The site of protein synthesis a) Polyribosomes- clusters of 5or 6 ribosomes working together to produce a protein b) Ribosomes are assembled in the nucleus and then enter the cytoplasm where they may attach to the RER or float free 4. Golgi Apparatus- layers of plate-like membranes which store and package cell products usually for transport out of the cell a) During storage, some materials are modified b) Proteins are packaged in a sac made from the membrane. The sacs are called secretory vessicles 5. Lysosome- golgi vesicle containing digestive enzymes that fuse with vacuoles and digest food a) Lysosomes fuse with food vacuoles to hydrolize proteins, polysaccarides and nucleic acids b) Autophagy- digesting cell components for fuel Organelles work together to carry out life processes 6. Mitochondria- power plant of the cell in which most of the reactions of cellular respiration occur a) Regulates calcium concentrations in the cell b) A double folded membrane of lipid bilayers is folded repeatedly into cristae (the center of mitochondrial activity) c) The matrix is the fluid that contains the enzymes for respiration 7. Plastids- organelles found only in plant cells that are involved in the synthesis or storage of food a) Chloroplast- contain chlorphyll and carotenoids which trap light energy for photosynthesis b) Leucoplast- plastid used in the storage of starch and other materials c) Chromoplast- contains pigments that give flowers and Fruits their characteristic colors Chloroplast 8. Peroxisomes- membranous sacs that contain oxidative enzymes A) Fatty acid oxidation occurs in peroxisomes B) Peoxisomes carry on metabolic reactions which break down H2O2 C) Detoxify compounds such as ethanol D) Involved in photorespiration in plants E) Glyoxysomes convert fatty acids to sugars in young plants 9. Microtubules • Hollow, spirally arrange tubes with walls of tubulin protein • Provide structural support and may have a role in cellular movement 10. Microfilaments Solid rod-like structure consisting of the contractile proteins actin and mysosin. Responsible for cell movement 11. Microtrabecular lattice- 3 dimensional interlinked microfilaments that form the Cytoskeleton or framework of the cell 12. Centrioles- hollow cylinder composed of nine triple microtubules that function in cell division. a) Anchor spindle fibers during cell division b) Centrioles are located within a dense area of cytoplasm called the centrosome near the nucleus 13. Vacuoles- membrane bound area for digestion, storage or waste disposal. The surrounding membrane is called the tonoplast 14. Cilia and Flagella- specialized microtubules used for movement a) Cilia usually occur in large numbers on the cell surface with a 9+2 pattern of microtubles. Cilia work together like oars on a boat b) Flagella- much longer and fewer in number. 9+2 arrangement. Flagella undulate or wiggle to produce movement E. Motor Molecules- convert ATP to movement 1. Kinesin- attaches to a vesicle at a receptor and “walks” along a microtubule 2. Dynein- side arms of dynein on microtubules of cilia and flagella crawl along neighboring microtubules causing them to bend 3. Actin and Myosin- interacting threads of myosin pull themselves along actin causing muscle contraction or other movement. E. The Cell Surface 1. Cell Wall- structure in plant, fungus, bacteria and some protists that provides strength and structure to a cell 2. Glycocalyx- fuzzy coat of oligosaccharides on the outer surface of some animal cells A) Provides strength to the cell membrane B) Helps join one cell to another 3. Intercellular Junctions A) Tight junctionsmembranes of neighboring cells are joined B) Desmosomes- rivit-like areas that anchor cells together C) Gap junctions-contain channels between adjacent cells