Arabidopsis Formin3 Directs the Formation of Actin
... their formation is unknown. The function of these actin cables, which are proposed to modulate cytoplasmic streaming and intracellular movement of many organelles in plants, has not been studied by genetic means. Here, we show that Arabidopsis thaliana formin3 (AFH3) is an actin nucleation factor re ...
... their formation is unknown. The function of these actin cables, which are proposed to modulate cytoplasmic streaming and intracellular movement of many organelles in plants, has not been studied by genetic means. Here, we show that Arabidopsis thaliana formin3 (AFH3) is an actin nucleation factor re ...
2004__MORRIS_et_al_Transpor... - Institute of Experimental Botany
... unlikely route for the transmission of a signal molecule involved in the finetuning of growth and development. Nevertheless, significant quantities of IAA occur in phloem sap and it is clear that the total amount of auxin that is delivered by the phloem to sink tissues is considerable. Consequently, ...
... unlikely route for the transmission of a signal molecule involved in the finetuning of growth and development. Nevertheless, significant quantities of IAA occur in phloem sap and it is clear that the total amount of auxin that is delivered by the phloem to sink tissues is considerable. Consequently, ...
Calcium and Plant Development - Labs
... (82 , 205, 206, 246). However, internal compartments such as the endoplasmic reticulum (ER) and vacuole (203, 220 , 250, 265 , 275) as well as mitochondria ( 1 95) may also provide a store of Ca2+ that can be released either through the direct action of the primary stimulus , e.g. light ( 1 95) , or ...
... (82 , 205, 206, 246). However, internal compartments such as the endoplasmic reticulum (ER) and vacuole (203, 220 , 250, 265 , 275) as well as mitochondria ( 1 95) may also provide a store of Ca2+ that can be released either through the direct action of the primary stimulus , e.g. light ( 1 95) , or ...
Auxin transport routes in plant development
... The plant hormone auxin (the predominant form of which is indole3-acetic acid; IAA) is a major coordinating signal in the regulation of plant development. Many aspects of auxin action depend on its differential distribution within plant tissues, where it forms local maxima or gradients between cells ...
... The plant hormone auxin (the predominant form of which is indole3-acetic acid; IAA) is a major coordinating signal in the regulation of plant development. Many aspects of auxin action depend on its differential distribution within plant tissues, where it forms local maxima or gradients between cells ...
Endocytosis, Recycling, and Regulated Exocytosis of Glucose
... has also been implicated in caveolin-1-, IL-2Rβ-, and flotillindependent endocytotic processes.15 GLUT4, the major carrier of glucose into muscle and fat cells, is a recycling protein that is continuously removed from and recycled back to the PM.5 Both arms of this process are subject to regulation ...
... has also been implicated in caveolin-1-, IL-2Rβ-, and flotillindependent endocytotic processes.15 GLUT4, the major carrier of glucose into muscle and fat cells, is a recycling protein that is continuously removed from and recycled back to the PM.5 Both arms of this process are subject to regulation ...
EAP actuators aid the quest for the "Holy Braille" of tactile displays
... Most of the thermally actuated braille display approaches require excessive peak drive power to quickly energize enough actuators to obtain reasonably short response or setup times. They also have problems associated with overheating due to accumulated heat dissipation of large numbers of closely pa ...
... Most of the thermally actuated braille display approaches require excessive peak drive power to quickly energize enough actuators to obtain reasonably short response or setup times. They also have problems associated with overheating due to accumulated heat dissipation of large numbers of closely pa ...
Sample pages 1 PDF
... The investigation of plant myosin had begun from attempting to elucidate the molecular mechanism of characean cytoplasmic streaming [82]. In 1970–1990, several proteins with ATPase activities had been isolated biochemically and reported as plant myosins (cited in [84]). However, none of these result ...
... The investigation of plant myosin had begun from attempting to elucidate the molecular mechanism of characean cytoplasmic streaming [82]. In 1970–1990, several proteins with ATPase activities had been isolated biochemically and reported as plant myosins (cited in [84]). However, none of these result ...
University of Groningen Polymerization of the bacterial cell division
... schematically depicted in Figure 1A, seems very simple yet is poorly understood on a molecular biological level. For the Gramnegative model organism Escherichia coli, numerous cell division genes were identified in E. coli temperature sensitive mutants cells that did not divide properly, and hence w ...
... schematically depicted in Figure 1A, seems very simple yet is poorly understood on a molecular biological level. For the Gramnegative model organism Escherichia coli, numerous cell division genes were identified in E. coli temperature sensitive mutants cells that did not divide properly, and hence w ...
Calcium at Fertilization and in Early Development
... through a src family kinase pathway and in mammals by the diffusion of a sperm-specific phospholipase C from sperm to egg on sperm-egg fusion. The fertilization calcium wave is then set into the context of cell cycle control, and the mechanism of repetitive calcium spiking in mammalian eggs is inves ...
... through a src family kinase pathway and in mammals by the diffusion of a sperm-specific phospholipase C from sperm to egg on sperm-egg fusion. The fertilization calcium wave is then set into the context of cell cycle control, and the mechanism of repetitive calcium spiking in mammalian eggs is inves ...
Functional analysis of parvin and different modes of IPP
... adhesion, similar to ILK and PINCH mutants. Furthermore, the identical muscle phenotype of the triple mutant, which for the first time in any organism removed the entire IPP-complex function, genetically demonstrated that parvin, ILK and PINCH function synergistically. This is consistent with the ti ...
... adhesion, similar to ILK and PINCH mutants. Furthermore, the identical muscle phenotype of the triple mutant, which for the first time in any organism removed the entire IPP-complex function, genetically demonstrated that parvin, ILK and PINCH function synergistically. This is consistent with the ti ...
Leukocyte lipid bodies — Biogenesis and functions in inflammation
... [21,22], and TIP 47 (tail-interacting protein of 47 kDa) [23]. These proteins have been implicated in lipid body assembly and biogenesis [21,22,24,25]. By using techniques of protein identification in subcellular lipid body-enriched fractionation combined with immunodetection of proteins by EM or li ...
... [21,22], and TIP 47 (tail-interacting protein of 47 kDa) [23]. These proteins have been implicated in lipid body assembly and biogenesis [21,22,24,25]. By using techniques of protein identification in subcellular lipid body-enriched fractionation combined with immunodetection of proteins by EM or li ...
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[pdf]
... the APC/C triggers substrate degradation by assembling K11-linked ubiquitin chains, the efficient formation of which depends on a surface of ubiquitin, the TEK-box. Strikingly, homologous TEK-boxes are found in APC/C substrates, where they facilitate chain nucleation. We propose that recognition of ...
... the APC/C triggers substrate degradation by assembling K11-linked ubiquitin chains, the efficient formation of which depends on a surface of ubiquitin, the TEK-box. Strikingly, homologous TEK-boxes are found in APC/C substrates, where they facilitate chain nucleation. We propose that recognition of ...
Stepwise Activation of BAX and BAK by tBID, BIM, and PUMA
... apoptosis. The permeabilization of the MOM not only couples the mitochondria to the activation of caspases but also initiates caspase-independent mitochondrial dysfunction (Cheng et al., 2001). The BCL-2 family proteins control a crucial checkpoint of apoptosis at the mitochondria (Cory and Adams, 2 ...
... apoptosis. The permeabilization of the MOM not only couples the mitochondria to the activation of caspases but also initiates caspase-independent mitochondrial dysfunction (Cheng et al., 2001). The BCL-2 family proteins control a crucial checkpoint of apoptosis at the mitochondria (Cory and Adams, 2 ...
PKC regulates the hypertrophic growth of cardiomyocytes through
... overexpression of other PKC isozymes. (C) Histogram representation of PKC particulate (open area) and cytosolic (dark area) protein levels (n 3 experiments). Adenoviral-mediated expression and redistribution of PKC was not reduced by coexpression of PKCII, , or (P 0.05). ...
... overexpression of other PKC isozymes. (C) Histogram representation of PKC particulate (open area) and cytosolic (dark area) protein levels (n 3 experiments). Adenoviral-mediated expression and redistribution of PKC was not reduced by coexpression of PKCII, , or (P 0.05). ...
Proceedings of the Indiana Academy of Science
... has been previously indicated autolytic enzymes are believed to aid in the process of destruction. In the residue of what was once intact cells, particles of various sizes appear. Dorfmen (28) measured these particles and considered them proportional to the rate of lysis. Particles from lysed, heat- ...
... has been previously indicated autolytic enzymes are believed to aid in the process of destruction. In the residue of what was once intact cells, particles of various sizes appear. Dorfmen (28) measured these particles and considered them proportional to the rate of lysis. Particles from lysed, heat- ...
SURVEY AND SUMMARY H1 histones
... Phosphorylation of histone H1 has many distinct functions, leading to both chromatin condensation and decondensation dependent on the site of phosphorylation and cell cycle context. Histone H1 phosphorylation has been shown to progressively increase as a cell progresses from G1 to mitosis during the ...
... Phosphorylation of histone H1 has many distinct functions, leading to both chromatin condensation and decondensation dependent on the site of phosphorylation and cell cycle context. Histone H1 phosphorylation has been shown to progressively increase as a cell progresses from G1 to mitosis during the ...
The RING Domain of Mdm2 Can Inhibit Cell
... and left on ice for 1 h. After sonication at 4°C (7 s ⫻ 2), cellular debris was removed by centrifugation in a microcentrifuge at 14,000 rpm for 15 min at 4°C. Proteins (200 g/lane) were electrophoretically separated on denaturing polyacrylamide gels containing SDS and transferred onto membranes (O ...
... and left on ice for 1 h. After sonication at 4°C (7 s ⫻ 2), cellular debris was removed by centrifugation in a microcentrifuge at 14,000 rpm for 15 min at 4°C. Proteins (200 g/lane) were electrophoretically separated on denaturing polyacrylamide gels containing SDS and transferred onto membranes (O ...
Donohoe, B.S., B. - University of Colorado-MCDB
... for COPII vesicle-mediated cargo transfer until the local supply of exportable cargo molecules has been exhausted and COPII bud formation stops. In support of this latter hypothesis, our electron tomography analysis of >50 plant Golgi has demonstrated that all of the ER-associated Golgi were docked ...
... for COPII vesicle-mediated cargo transfer until the local supply of exportable cargo molecules has been exhausted and COPII bud formation stops. In support of this latter hypothesis, our electron tomography analysis of >50 plant Golgi has demonstrated that all of the ER-associated Golgi were docked ...
Arabidopsis VILLIN4 is involved in root hair growth through
... 1999). Both P-135-ABP and P-115-ABP colocalize with actin filament bundles in the transvacuolar strands and the subcortical regions within the root hair cells of Hydrocharis dubia (Tominaga et al., 2000; Yokota et al., 2003). Microinjection of the antiserum against P-115-ABP or P-135-ABP into living ...
... 1999). Both P-135-ABP and P-115-ABP colocalize with actin filament bundles in the transvacuolar strands and the subcortical regions within the root hair cells of Hydrocharis dubia (Tominaga et al., 2000; Yokota et al., 2003). Microinjection of the antiserum against P-115-ABP or P-135-ABP into living ...
Decision making by p53: life, death and cancer
... phosphorylation of p53 on serine 46 will bias the transcriptional repertoire of p53 towards proapoptotic genes, thereby favoring death. An additional twist is introduced by the finding that PPM1D/Wip1, a phosphatase that inhibits serine 46 phosphorylation, is a positive transcriptional target of p53 ...
... phosphorylation of p53 on serine 46 will bias the transcriptional repertoire of p53 towards proapoptotic genes, thereby favoring death. An additional twist is introduced by the finding that PPM1D/Wip1, a phosphatase that inhibits serine 46 phosphorylation, is a positive transcriptional target of p53 ...
Plant hormones and growth regulators
... which bind Ca2+. It follows that low [Ca2+]e should make the cell wall more pliable and easily ruptured, whereas high concentrations should rigidify the wall and make it less plastic. It had become apparent in the mid to late fifties that modifying the [Ca2+]e produced a pronounced effect on cell gr ...
... which bind Ca2+. It follows that low [Ca2+]e should make the cell wall more pliable and easily ruptured, whereas high concentrations should rigidify the wall and make it less plastic. It had become apparent in the mid to late fifties that modifying the [Ca2+]e produced a pronounced effect on cell gr ...
Development of in vitro procedures that can predict the safety of
... (mAb) TGN1412 (developed for the treatment of autoimmune diseases) failed to predict the life threatening adverse events that occurred during its Phase I Clinical Trial. The treatment of disease using mAb therapy is becoming increasingly common, so, to ensure the safety of mAbs, pre-clinical safety ...
... (mAb) TGN1412 (developed for the treatment of autoimmune diseases) failed to predict the life threatening adverse events that occurred during its Phase I Clinical Trial. The treatment of disease using mAb therapy is becoming increasingly common, so, to ensure the safety of mAbs, pre-clinical safety ...
NCAM Polypeptides in Heart Development: Association with Z Discs
... endocardial endothelium; in contrast, N-cadherin is expressed only in the myocardium at this stage of heart development (Linask, 1992). Concomitantly with their dcadhcsion, migrating E C T ceils downregulate their expression of N C A M (Mjaatvedt and Markwald, 1989; Crossin and Hoffman, 1991) as ass ...
... endocardial endothelium; in contrast, N-cadherin is expressed only in the myocardium at this stage of heart development (Linask, 1992). Concomitantly with their dcadhcsion, migrating E C T ceils downregulate their expression of N C A M (Mjaatvedt and Markwald, 1989; Crossin and Hoffman, 1991) as ass ...
Maternal mRNAs are regulated by diverse P body
... in late-stage oocytes might be controlled by sperm-derived signals (Lublin and Evans, 2007). To test this, we compared PUF-5 expression in wild-type hermaphrodite gonads to gonads from fog-2(q71) females; fog-2 mutations prevent sperm formation in otherwise normal XX animals (Schedl and Kimble, 1988 ...
... in late-stage oocytes might be controlled by sperm-derived signals (Lublin and Evans, 2007). To test this, we compared PUF-5 expression in wild-type hermaphrodite gonads to gonads from fog-2(q71) females; fog-2 mutations prevent sperm formation in otherwise normal XX animals (Schedl and Kimble, 1988 ...