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title of the thesis - UWSpace
title of the thesis - UWSpace

... gastrulation. The spatial and temporal regulation of cell movement requires a highly coordinated cross-talk between cadherin and integrin adhesion receptors. While the adhesive properties and morphogenetic movements are well described, the molecular mechanisms controlling these behaviors remain unkn ...
HELICOBACTER PYLORI VacA, A PARADIGM FOR TOXIN
HELICOBACTER PYLORI VacA, A PARADIGM FOR TOXIN

... The importance of bacterial toxins in infectious diseases was recognized more than a century ago, when it was discovered that culture filtrates of many pathogens contain soluble factors that can damage host tissues1. Over the past century, more than one hundred different toxins that are produced by ...
Endogenous Drp1 Mediates Mitochondrial Autophagy and Protects
Endogenous Drp1 Mediates Mitochondrial Autophagy and Protects

... Drp1 plays an essential role in mediating Parkin-induced mitochondria selective autophagy, namely mitophagy in MEF cells 15. Drp1 also mediates Bnip3-induced autophagy in adult CMs 16. However, whether Drp1 is involved in general autophagy that can remove mitochondria (which we here referred to as m ...
Oxidative-stress-induced nuclear to cytoplasmic relocalization is
Oxidative-stress-induced nuclear to cytoplasmic relocalization is

The dehydratase ADT3 affects ROS homeostasis
The dehydratase ADT3 affects ROS homeostasis

... cells, which lack oil bodies and normal plastids. Interestingly, upregulation of the pathway ...
Live Imaging of Drosophila Brain Neuroblasts Reveals a Role for
Live Imaging of Drosophila Brain Neuroblasts Reveals a Role for

... cortex in humans. Lis1 binds dynactin and the dynein motor complex, but the role of Lis1 in many dynein/dynactindependent processes is not clearly understood. Here we generate and/or characterize mutants for Drosophila Lis1 and a dynactin subunit, Glued, to investigate the role of Lis1/dynactin in m ...
Ectopic Expression of AINTEGUMENTA in Arabidopsis Plants
Ectopic Expression of AINTEGUMENTA in Arabidopsis Plants

... cells called floral meristems. Floral organ primordia arise at defined positions from within these meristems, grow, and eventually differentiate into the four organs of a flower (sepals, petals, stamens, and carpels). As plant cells do not undergo migration, cell division and cell expansion are the ...
YEAST INFORMATION SHEET
YEAST INFORMATION SHEET

... for inoculating the next batch. ...
Dominant-Negative Receptor Uncovers
Dominant-Negative Receptor Uncovers

... mutations may not have an erecta-like phenotype; instead, they may display no phenotype at all, a subset of the erecta phenotypes, more severe phenotypes, or even be lethal. From the same genetic screen, erecta-like4 (elk4) was identified as a silique-specific genetic interactor of erecta, suggestin ...
Dramatic increase in lymph node dendritic cell
Dramatic increase in lymph node dendritic cell

... A DC-enriched cell fraction was then obtained after depletion of T cells, B cells, and granulocytes by treating the 1.061-density fraction for 50 minutes at 4°C with a monoclonal antibody (mAb) mixture including anti-CD3 (clone KT3-1.1), anti-B220 (clone RA3-6B2), and antigranulocyte antigen Gr1 (cl ...
1 Sister chromatids are often incompletely cohesed
1 Sister chromatids are often incompletely cohesed

... During preliminary investigations we occasionally found three or four instead of one or two FISH signals for chromosome-specific ~100 kb segments in 4C nuclei of A. thaliana indicating that not only homologues but also sister chromatids may occupy separate positions within a nucleus. Therefore, we b ...
Specific Organization of Golgi Apparatus in Plant Cells
Specific Organization of Golgi Apparatus in Plant Cells

... occurs along cytoskeleton fibrils; however, over long distances organelles are moved along actin filaments with involvement of “rapid” myosins, whereas the microtubules are responsible for “anchoring” or deceleration of the movement of organelles and, possibly, for their transfer over small distance ...
A single-headed fission yeast myosin V transports actin in a
A single-headed fission yeast myosin V transports actin in a

... Va myosin from vertebrates is shown for comparison. To directly show the state of oligomerization of Myo51, we visualized the motor by EM of negatively stained samples (Fig. S1). The images showed a single-headed motor, consistent with the low predicted coiled-coil propensity of the tail. Representa ...
Soluble factors from human endometrium promote angiogenesis
Soluble factors from human endometrium promote angiogenesis

Characterization of the Nucleolar Gene Product, Treacle, in Treacher Collins Syndrome
Characterization of the Nucleolar Gene Product, Treacle, in Treacher Collins Syndrome

... amino acids shorter than its human ortholog, [35S]methionine- and [35S]cysteine-labeled mouse treacle migrated as a single band of ⬃220 kDa, confirming the aberrant mobility of treacle on SDS-PAGE. This unusual behavior was very reminiscent of the treacle-related protein Nopp140, which migrates as a ...
Isolation and characterization of the Pin1/Ess1p homologue in
Isolation and characterization of the Pin1/Ess1p homologue in

... to select for Ade+ cells, indicative of diploid cells. The KpnI-SacII DNA fragment from p87 containing deletion construct was purified from agarose gel and transformed into diploid cells. His+ cells were selected and subjected to tetrad analysis. Spores with a His+ phenotype (Sp23 and Sp25), indicat ...
Costes et al.
Costes et al.

Making a Connection: Direct Binding between
Making a Connection: Direct Binding between

Document
Document

... (b) removal of toxic macromolecular aggregates too large for handling by smaller capacity or single-molecule-handling proteolytic systems of the cell (e.g. proteasome); ...
iv Molecular Mechanisms of Notochord Vacuole
iv Molecular Mechanisms of Notochord Vacuole

... vertebrate development. At the center of the vertebrate notochord is a large fluidfilled organelle, the notochord vacuole. While these highly conserved intracellular structures have been described for decades, little is known about the molecular mechanisms involved in their biogenesis and maintenanc ...
Thesis titel 1.5 final - SciDok
Thesis titel 1.5 final - SciDok

... material revealed the influence of different material properties on the growth of the Chorio-Alantoic Membrane (CAM), which is crucial for embryonic growth and development. Conclusion: This thesis is the first step towards the overall aim t develop an artificial egg as in vitro cell culture system a ...
EGFR/Ras Signaling Controls Drosophila Intestinal Stem
EGFR/Ras Signaling Controls Drosophila Intestinal Stem

Photoactivatable GFP tagging cassettes for protein
Photoactivatable GFP tagging cassettes for protein

... upstream of the stop codon of ERG6 as the genespecific sequence in the forward F2 primer for homologous integration. The reading frame of the PA–GFP tag was preserved and a linker sequence encoding GlyAlaGly was engineered in the F2 primer, separating the last codon of ERG6 from the first codon of P ...
The TSC1–TSC2 complex: a molecular switchboard controlling cell
The TSC1–TSC2 complex: a molecular switchboard controlling cell

... These studies demonstrated that the TSC1–TSC2 complex acts as an upstream inhibitor of S6K, perhaps through inhibition of TOR signalling. TOR proteins are serine/threonine kinases of the PIKK (phosphoinositide 3-kinase-related kinase) family, with orthologues found in all eukaryotes. TOR proteins pl ...
Fibronectin and Other Adhesive Glycoproteins
Fibronectin and Other Adhesive Glycoproteins

... cells in embryos and adult tissues (Hynes 1990; Mosher 1989). Plasma fibronectin is synthesized in the liver by hepatocytes and present in a soluble form in blood plasma at a concentration of around 300 mg/ml. Cellular fibronectin is secreted by fibroblasts and multiple other cell types and is organ ...
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Cell growth

The term cell growth is used in the contexts of cell development and cell division (reproduction). When used in the context of cell division, it refers to growth of cell populations, where a cell, known as the ""mother cell"", grows and divides to produce two ""daughter cells"" (M phase). When used in the context of cell development, the term refers to increase in cytoplasmic and organelle volume (G1 phase), as well as increase in genetic material (G2 phase) following the replication during S phase.
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