Glutamate 83 and arginine 85 of helix H3 bend are key residues for
... sequential addition of at least twelve other membrane proteins responsible for peptidoglycan assembly and membrane constriction [3]. Inactivation of the corresponding genes blocks the cell division and give rise to long multinucleated cells that fail to propagate and eventually die [4]. The Z-ring a ...
... sequential addition of at least twelve other membrane proteins responsible for peptidoglycan assembly and membrane constriction [3]. Inactivation of the corresponding genes blocks the cell division and give rise to long multinucleated cells that fail to propagate and eventually die [4]. The Z-ring a ...
Inositol trisphosphate and calcium oscillations
... extent either by the addition of higher doses of 5-HT or by increasing the external level of Ca2+. I then measured the accumulation of InsP1 under these two conditions. If InsP3 formation is driving each transient, the expectation would be for the turnover of inositol phosphates to increase for both ...
... extent either by the addition of higher doses of 5-HT or by increasing the external level of Ca2+. I then measured the accumulation of InsP1 under these two conditions. If InsP3 formation is driving each transient, the expectation would be for the turnover of inositol phosphates to increase for both ...
The Populus homeobox gene ARBORKNOX2 regulates
... stem (Mele et al., 2003). Importantly, the expression of key cell wall synthesis-related genes show negative correlation with BP expression levels, and BP protein binds to the promoters of some of these genes in vitro (Mele et al., 2003). Partially redundant roles of STM and BP are revealed by as1 s ...
... stem (Mele et al., 2003). Importantly, the expression of key cell wall synthesis-related genes show negative correlation with BP expression levels, and BP protein binds to the promoters of some of these genes in vitro (Mele et al., 2003). Partially redundant roles of STM and BP are revealed by as1 s ...
TETRASPORE is required for male meiotic
... condensed chromatin and few organelles, and a larger vegetative cell. The generative cell moves away from the inner wall of the microspore, becoming completely enclosed in the vegetative cytoplasm, and at pollen mitosis II (PMII) divides to form two sperm. In some species, including Arabidopsis, PMI ...
... condensed chromatin and few organelles, and a larger vegetative cell. The generative cell moves away from the inner wall of the microspore, becoming completely enclosed in the vegetative cytoplasm, and at pollen mitosis II (PMII) divides to form two sperm. In some species, including Arabidopsis, PMI ...
TETRASPORE is required for male meiotic
... 2648 M. Spielman and others walls (Worrall et al., 1992; Peirson et al., 1996). In these, membranes or non-callosic walls separate the microspores, while in tes no intersporal membranes or walls are observed in the absence of callose. Secondly, TES is also unlikely to encode a product required for ...
... 2648 M. Spielman and others walls (Worrall et al., 1992; Peirson et al., 1996). In these, membranes or non-callosic walls separate the microspores, while in tes no intersporal membranes or walls are observed in the absence of callose. Secondly, TES is also unlikely to encode a product required for ...
Downloaded - The Journal of Cell Biology
... Finally, there are multiple splice forms of p120. This complexity makes genetic analysis in mammals challenging, with full loss of function likely requiring the generation of dou- ...
... Finally, there are multiple splice forms of p120. This complexity makes genetic analysis in mammals challenging, with full loss of function likely requiring the generation of dou- ...
Reactive oxygen species are linked to the toxicity of the
... experiments. The cultures were not axenic, but asepThe bioactive, allelochemical compounds protic techniques were used to minimize additional duced by Alexandrium spp. affect the structure and microbial contamination. Prior to experimentation, function of cell membranes, causing immobilization Alexa ...
... experiments. The cultures were not axenic, but asepThe bioactive, allelochemical compounds protic techniques were used to minimize additional duced by Alexandrium spp. affect the structure and microbial contamination. Prior to experimentation, function of cell membranes, causing immobilization Alexa ...
PDF
... large amount of unglycosylated protein for structural studies, glycosylation-deficient mutants were generated in which Asn residues at positions 91, 94 and 99 were either replaced with Gln (Gly−), Asp (Gly− D), or all three Asn were deleted (Gly− ⌬). The biochemical properties of the mutant protein, ...
... large amount of unglycosylated protein for structural studies, glycosylation-deficient mutants were generated in which Asn residues at positions 91, 94 and 99 were either replaced with Gln (Gly−), Asp (Gly− D), or all three Asn were deleted (Gly− ⌬). The biochemical properties of the mutant protein, ...
Chitin and Chitosan in Fungi - Wiley-VCH
... walls of the Oomycete Pythium ultimum contain cellulose and chitin, whereas only cellulose is present in another Oomycete, Phythophthora parasitica. Both polysaccharides are present in cell walls of the Ascomycetes Ophiostoma ulmi and Colletotrichum lindemuthianum, whereas the Ascomycete Fusarium ox ...
... walls of the Oomycete Pythium ultimum contain cellulose and chitin, whereas only cellulose is present in another Oomycete, Phythophthora parasitica. Both polysaccharides are present in cell walls of the Ascomycetes Ophiostoma ulmi and Colletotrichum lindemuthianum, whereas the Ascomycete Fusarium ox ...
Cdc6 in S phase in human cells - Journal of Cell Science
... We first asked whether Cdc6 enters the nucleus under in vitro replication conditions. We chose to use purified recombinant Xenopus Cdc6 protein in these studies for three reasons. Firstly, because we know it stimulates replication in mammalian nuclei in vitro (Stoeber et al., 1998), secondly because ...
... We first asked whether Cdc6 enters the nucleus under in vitro replication conditions. We chose to use purified recombinant Xenopus Cdc6 protein in these studies for three reasons. Firstly, because we know it stimulates replication in mammalian nuclei in vitro (Stoeber et al., 1998), secondly because ...
The role of yolk syncytial layer and blastoderm movements during
... zygote, segregating it from the large yolk mass. About 30 minutes after fertilization, the blastodisc has formed at the animal pole, while a big yolk mass constitutes the vegetal portion of the egg. During early cleavage stages, the zygote starts to divide in a discoidal-meroblastic manner, with mar ...
... zygote, segregating it from the large yolk mass. About 30 minutes after fertilization, the blastodisc has formed at the animal pole, while a big yolk mass constitutes the vegetal portion of the egg. During early cleavage stages, the zygote starts to divide in a discoidal-meroblastic manner, with mar ...
Endoplasmic Microtubules Configure the Subapical Cytoplasm and
... MT-inhibiting drugs oryzalin and taxol gave evidence that EMTs are essential in maintaining the specific cytoarchitecture of growing root hairs, including a fixed distance between nucleus and hair tip, as well as to keep the growth rate of these hairs at a high level. RESULTS ...
... MT-inhibiting drugs oryzalin and taxol gave evidence that EMTs are essential in maintaining the specific cytoarchitecture of growing root hairs, including a fixed distance between nucleus and hair tip, as well as to keep the growth rate of these hairs at a high level. RESULTS ...
Full Text - Labs / Projects - Fred Hutchinson Cancer Research Center
... precise mechanisms involved are not yet known (Anastasiadis and Reynolds, 2000; Noren et al., 2000). In addition to their roles at adherens junctions, β-catenin and p120ctn function in the nucleus in conjunction with transcription factors to regulate gene expression (Behrens et al., 1996; Daniel and ...
... precise mechanisms involved are not yet known (Anastasiadis and Reynolds, 2000; Noren et al., 2000). In addition to their roles at adherens junctions, β-catenin and p120ctn function in the nucleus in conjunction with transcription factors to regulate gene expression (Behrens et al., 1996; Daniel and ...
Auxin coordinates cell division and cell fate specification during
... could link auxin to the molecular regulation of cell cycle. However, on top of this complexity, it could be that some auxin responses are confined to certain stages of the cell cycle or in other words that cells might acquire the capacity to respond to auxin only at specific phases of the cell cycle ...
... could link auxin to the molecular regulation of cell cycle. However, on top of this complexity, it could be that some auxin responses are confined to certain stages of the cell cycle or in other words that cells might acquire the capacity to respond to auxin only at specific phases of the cell cycle ...
Auxin coordinates cell division and cell fate specification during
... could link auxin to the molecular regulation of cell cycle. However, on top of this complexity, it could be that some auxin responses are confined to certain stages of the cell cycle or in other words that cells might acquire the capacity to respond to auxin only at specific phases of the cell cycle ...
... could link auxin to the molecular regulation of cell cycle. However, on top of this complexity, it could be that some auxin responses are confined to certain stages of the cell cycle or in other words that cells might acquire the capacity to respond to auxin only at specific phases of the cell cycle ...
Polyphosphates of the Yeast Cell Vacuole
... during these experiments were analysed by gel filtration on Sephadex G-25. There was no evidence of exclusion of the labelled polyphosphates of' either the vacuolar or cytoplasmic fractions from the gel. Thus the 32P-polyphosphatessynthesized by the protoplasts in these experiments were unlike those ...
... during these experiments were analysed by gel filtration on Sephadex G-25. There was no evidence of exclusion of the labelled polyphosphates of' either the vacuolar or cytoplasmic fractions from the gel. Thus the 32P-polyphosphatessynthesized by the protoplasts in these experiments were unlike those ...
Interaction of PIN and PGP transport mechanisms in auxin
... PGP19-HA (GVG-PGP19-HA) or PIN7 (GVG-PIN7) constructs that had already been used to study the ability of corresponding proteins to mediate efflux from plant cells (Petrášek et al., 2006). After induction of GVG-PGP19-HA or GVG-PIN7 constructs, cells accumulated less auxin because of the increased au ...
... PGP19-HA (GVG-PGP19-HA) or PIN7 (GVG-PIN7) constructs that had already been used to study the ability of corresponding proteins to mediate efflux from plant cells (Petrášek et al., 2006). After induction of GVG-PGP19-HA or GVG-PIN7 constructs, cells accumulated less auxin because of the increased au ...
Mitochondrial pleomorphy in plant cells is driven
... 2010). Our present mechanistic understanding of mitochondria dynamics relies heavily on the relative frequency with which mitochondrial fission vs. fusion takes place in a cell. Frequent fission results in small mitochondria whereas a reduced fission frequency or an increased tendency to fuse leads ...
... 2010). Our present mechanistic understanding of mitochondria dynamics relies heavily on the relative frequency with which mitochondrial fission vs. fusion takes place in a cell. Frequent fission results in small mitochondria whereas a reduced fission frequency or an increased tendency to fuse leads ...
High-resolution imaging of cardiomyocyte behavior
... a form of heart failure (Jenni et al., 1999). The trabeculae serve to increase myocardial mass before the formation of coronary vessels and, as such, have been shown to contribute to ventricular contractility; they are also necessary for the formation of the conduction system (Sedmera et al., 2003; ...
... a form of heart failure (Jenni et al., 1999). The trabeculae serve to increase myocardial mass before the formation of coronary vessels and, as such, have been shown to contribute to ventricular contractility; they are also necessary for the formation of the conduction system (Sedmera et al., 2003; ...
The 21-day postnatal rat ventricular cardiac muscle cell in culture as
... The purpose of this study was to develop and characterize a cardiomyocyte culture system for use as an experimental model to study the mechanism(s) by which cardiac muscle cells permanently exit the cell cycle during early neonatal life. Ventricular cardiomyocytes, isolated by retrograde perfusion o ...
... The purpose of this study was to develop and characterize a cardiomyocyte culture system for use as an experimental model to study the mechanism(s) by which cardiac muscle cells permanently exit the cell cycle during early neonatal life. Ventricular cardiomyocytes, isolated by retrograde perfusion o ...
Conditional expression of Mycobacterium smegmatis
... analyses revealed that DnaA accounts for approximately 018 % of the total cellular protein during both the active and stationary growth periods. Expression of antisense dnaA RNA reduced viability, indicating that dnaA is an essential gene in replication. To further understand the role(s) of dnaA in ...
... analyses revealed that DnaA accounts for approximately 018 % of the total cellular protein during both the active and stationary growth periods. Expression of antisense dnaA RNA reduced viability, indicating that dnaA is an essential gene in replication. To further understand the role(s) of dnaA in ...
golgi apparatus, gerl, and lysosomes of neurons in rat dorsal root
... mature zymogen is ultimately released to the acinar lumen by fusion of the vacuole membranes with the plasma membrane . Electron microscope evidence from many laboratories suggests that in a wide variety of animal and plant cell types condensation of secretory and lysosomal proteins occurs in the sa ...
... mature zymogen is ultimately released to the acinar lumen by fusion of the vacuole membranes with the plasma membrane . Electron microscope evidence from many laboratories suggests that in a wide variety of animal and plant cell types condensation of secretory and lysosomal proteins occurs in the sa ...