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BIO 30 CH 18 MOLECULAR GENETICS REVIEW Multiple Choice Identify the choice that best completes the statement or answers the question. ____ 1. A molecule of DNA has a sequence of bases that read ATTCAGTAC. The base sequence of the complimentary strand is A. ATTCAGTAC B. TAACTGATC C. TAAGTCATG D. CATGACTTA Figure 20.1 DNA Strand ____ 2. Use the Figure 20.1 to answer the following question. The molecule being copied from the DNA strand represents A. complimentary DNA B. rRNA C. tRNA D. mRNA ____ 3. Use the Figure 20.1 to answer the following question. The diagram illustrates the process of A. replication B. transcription C. translation D. duplication ____ 4. Which of the following statements related to the roles of various molecules during the process of protein synthesis are correct. A. The tRNA molecule is the site where the DNA code is translated. B. The mRNA is a copy of a section of the DNA code. C. The tRNA carries the DNA message from the nucleus to the ribosome. D. The rRNA is the molecule which transports the amino acids to the ribosome. Figure 20.2 ____ 5. Use Figure 20.2 to answer the following question. The structures labeled X, Y and Z respectively represent A. mRNA, codon, ribosome B. mRNA, protein molecule, tRNA C. mRNA, tRNA, ribosome D. DNA, codon, mRNA ____ 6. Use Figure 20.2 to answer the following question. The diagram illustrates the process of A. transcription B. translation C. replication D. duplication ____ 7. Use Figure 20.2 to answer the following question. The function of the molecule labeled Y is to A. transport amino acids to the ribosome B. transport the DNA code to the ribosome C. identify mutations in the DNA sequence D. carry out the process of transcription ____ 8. In an experiment, a group of rabbits were given an injection of a radioactive isotope. Another group was exposed to the same dosage of radiation, but over the period of a few weeks. Significantly lower mutations were found in the group exposed to radiation for a longer period of time. This is due to the fact that A. a repair mechanism for mutations in the DNA is at work. B. it is the length of time and not the amount of radiation that is significant. C. small doses of radiation are not harmful. D. small doses of radiation does not effect bases in DNA. Scenario 20.1 Mustard Gas Mustard gas was used during the First World War causing the painful deaths of many soldiers. The chemical in mustard gas causes mutations to occur in the DNA lining the respiratory system Original sequence of nucleotides in a DNA molecule. CAT CAG TTC AGC Sequence of nucleotides after exposure to mustard gas. CAT CAG CTC AGC ____ 9. Use Scenario 20.1 to answer the following question. The type of gene mutation shown in the above example is A. reversion B. substitution C. deletion D. addition ____ 10. Use Scenario 20.1 to answer the following question. In the mutated DNA sequence, the amino acid that is switched in the sequence is A. leucine to valine B. lysine to glutamic acid C. lysine to valine D. leucine to glutamic acid ____ 11. Suppose that a mutation occurred where a base pair in the DNA molecule was added. This would cause the codons to be shifted over by one base molecule. This would result in A. the addition of an amino acid in the protein synthesized. B. a change in the reading frame of the entire chromosome. C. a change in the reading frame for that gene. D. deletion of an amino acid in the protein synthesized. Example 20.1 Portion of Insulin Protein Phenylalanine - Valine - Asparagine - Glutamine - Histidine ____ 12. Use Example 20.1 to answer the following question. The strand of DNA that would code for this portion of insulin is A. CAC GAG AAC GTA TTC B. TTC GTA AAC GAG CAC C. AAG CAA TTA GTT GTA D. AAA CAA TTC CAC CTA ____ ____ 13. Some of the substances that are required for protein synthesis to occur are A. salts and amino acids B. enzymes and amino acids C. enzymes and salts D. fats and amino acids 14. A codon can be described as A. B. C. D. the code for one amino acid a nucleotide with 3 bases a code for a protein a nucleotide Scenario 20.3 Huntingtons Disease In an individual with Huntingtons disease, part of the coding strand of DNA that has been deleted has the following base sequence. ATT CAG GAT TTT ACG ____ 15. Use Scenario 20.3 to answer the following question . The second amino acid coded for by this short segment of DNA is A. glutamine B. aspartic acid C. leucine D. valine Scenario 20.2 Breast Milk A study published in the journal Pediatrics shows that infants who are breast fed have a much lower risk of developing diarrhea when compared to infants who are fed formula. A different study reports that while many infants contain bacteria in their digestive system that would cause diarrhea in adults, breast fed infants do not become ill. A bacterium specific to the human digestive system produces a toxin which irritates the lining of the digestive system resulting in diarrhea. Breast milk contains a protein which binds to the toxin produced by the bacteria resulting in it being ineffective. ____ 16. Use Scenario 20.2 to answer the following question. The protein found in breast milk is produced when A. DNA is replicated B. DNA is translated C. mRNA is translated D. mRNA is replicated ____ 17. Use Scenario 20.2 to answer the following question. The toxin produced by the bacteria that is responsible for the development of diarrhea is produced by a section of DNA that has the code CCT TAT CAG. The sequence of amino acids produced by this sequence is A. glycine - isoleucine - aspartic acid B. glycine - isoleucine - valine C. glycine - tyrosine - valine D. glycine - tyrosine - isoleucine ____ 18. Use Scenario 20.2 to answer the following question. The number of codons shown by the section of DNA coding for the protein found in breast milk that contains the base sequence ACTTACCAG is A. 4 B. 9 C. 3 D. 1 ____ 19. A code of 5 amino acids contains a DNA sequence of A. 5 bases B. 10 bases C. 15 bases D. 25 bases ____ 20. In protein synthesis, tRNA A. initiates the transcription process. B. collects and transfers amino acids. C. ensures that the correct codons are translated into amino acids. D. acts as the site for the synthesis of an amino acid chain. ____ 21. Which of the following structures is composed of a specific sequence of amino acids? A. DNA B. RNA C. nucleotide D. polypeptide chain ____ 22. An anticodon consists of three A. amino acids attached to rRNA B. DNA nitrogen bases C. mRNA nitrogen bases D. tRNA nitrogen bases ____ 23. HIV is a retrovirus which is a class of viruses that has its traits encoded by RNA instead of DNA. Retroviruses utilize an enzyme called reverse transcriptase which acts by invading the host cell’s DNA. The function of reverse transcriptase is to A. prevent DNA replication B. prevent protein synthesis C. transcribe RNA into DNA D. transcribe DNA into RNA Figure 20.3 DNA Replication ____ 24. Use Figure 20.3 to answer the following question. The process shown by the diagram occurs in A. cell division B. transcription C. translation D. recombination ____ 25. Initiators and terminators (start and stop codons) are important to protein synthesis because they specify A. where the mRNA chain should start and finish. B. which amino acids should be included in the protein. C. where the amino acids should be added at the ribosome. D. when the DNA replication begins and ends. ____ 26. Since the first nucleotides cannot be linked in a newly synthesized strand in DNA replication, __________ is required. A. an RNA primer B. a DNA primer C. DNA polymerase D. ligase ____ 27. The use of recombinant DNA technology combines DNA from one organism into the DNA of another organism. This process is achieved by the use of A. restriction enzymes and ligase. B. polymerase and helicase. C. polymerase and ligase. D. restriction enzymes and helicase. ____ 28. The process of cloning involves taking DNA from a body cell of one organism and inserting it into an enucleated egg cell from a donor. The zygote produced is then inserted into a surrogate mother. The amount of DNA that the clone shares with the surrogate mother is A. 0%. B. 50%. C. 75%. D. 100%. ____ 29. Unlike DNA, an RNA molecule A. contains uracil. B. contains deoxyribose sugar. C. moves from the cytoplasm to the nucleus. D. is double stranded. Figure 20.4 DNA Replication II ____ 30. Use Figure 20.4 to answer the following question. The diagram illustrates the process of A. replication B. translation C. transcription D. duplication ____ 31. The following statements are related to the various roles molecules play during the process of protein synthesis. 1. The rRNA molecule carries amino acids to the ribosome. 2. The tRNA is where the code is translated. 3. The mRNA is a copy of the DNA molecule. 4. The mRNA carries the message from the nucleus to the ribosomes. Which of the statements are correct? A. 1 and 2 B. 1 and 3 C. 2 and 3 D. 3 and 4 ____ 32. The following statements represent the roles of various molecules during the process of protein synthesis. 1. Amino acids are linked to form a polypeptide. 2. The genetic code of the DNA is transcribed. 3. Matching of the anticodon of the tRNA with the codon of the RNA. 4. The DNA molecule unwinds and unzips. Which of the following represents the sequence of events that occurs as a protein is synthesized? A. 2,3,1,4 B. 4,2,3,1 C. 4,3,1,2 D. 4,3,2,1 ____ 33. A student was asked to build a molecule of mRNA codon from the DNA codon ATT. The student was given the following list of substances to work with for their model. 1. thymine 2. ribose sugar 3. deoxyribose sugar 4. uracil 5. phosphate 6. adenine To form the nucleotides of the RNA codon, the molecule must include the substances numbered A. 1,2,5,6 B. 1,3,5,6 C. 2,4,5,6 D. 3,4,5,6 ____ 34. Which of the following are enzymes involved in posttranscriptional modification? A. RNA polymerase and spliceosomes B. RNA polymerase and poly-A polymerase C. poly-A polymerase and spliceosomes D. RNA polymerase, poly-A polymerase and spliceosomes ____ 35. What does an operon typically consist of? A. an operator, a promoter, and a cluster of genes B. a cluster of genes C. a promoter and a cluster of genes D. an operator and a cluster of genes Completion Complete each statement. 1. The nucleotides of a DNA molecule and an RNA molecule differ as DNA contains thymine and RNA contains ______________________. 2. The process of transcription involves converting the DNA code into a molecule of ____________________. 3. Mutations are changes in the DNA that result in the incorrect sequence of _______________________ in the development of a protein. 4. When a molecule of DNA is replicated, the base sequences ACAATG is copied to make a complimentary strand with the sequence ___________________. 5. The start codon found at the beginning of every mRNA molecule is ___________________. 6. Transcription occurs through the processes of initiation, elongation and ____________________. 7. The DNA sequence ACC TAG codes for the amino acids ____________________ and ____________________. 8. The DNA of eukaryotic organisms contains coding regions called ____________________ and non coding regions called ____________________. 9. The role of bringing amino acids to the ribosome during the process of translation belongs to the _________________________. 10. A mutation that causes the reading frame of codons to change resulting in the incorporation of different amino acids into the sequence is called a ____________________ mutation. Matching Match each item with the correct statement below. A. mRNA E. B. tRNA F. C. amino acid G. D. RNA Polymerase H. codon transcription translation mutation ____ 1. Molecules that are linked together to form a polypeptide. ____ 2. DNA copy used to create an amino acid sequence. ____ 3. Puts together the appropriate nucleotides to build an mRNA molecule. ____ 4. Moves amino acids from the cytoplasm to the ribosome to build the protein. ____ 5. Errors made in the DNA sequence that are inherited. ____ 6. A triplet of nucleotides that codes for an amino acid. ____ 7. The conversion of a DNA sequence into an mRNA sequence. ____ 8. The conversion of an mRNA sequence into an amino acid sequence. Short Answer 1. Fill in the table that gives the base sequence of a section of a DNA molecule. Assume that the code is being read from left to right, and that the upper strand of the DNA code is being transcribed. C DNA C T U A Trp G A mRNA tRNA amino acids 2. What is the function of each of the following in protein synthesis: tRNA, mRNA, rRNA? 3. Differentiate between transcription and translation. 4. The following polypeptide sequence has been obtained from a protein found in eukaryotic cells. met-gly-pro-val-arg a) List a possible mRNA sequence that may have coded for this protein. b) Explain why more than 1 sequence may be possible. c) How can variability in mRNA sequence due to mutation result in the same sequence of amino acids? 5. While DNA replication and transcription are similar process, there are significant differences between the two. Provide two differences between the processes. 6. Poisonous mushrooms contain a substance called alpha-amanitin. Trace amounts of this substance stop body cells from synthesizing mRNA. Explain why eating these mushrooms could be fatal. 7. Children suffering from thalassemia are homozygous for a gene that is involved in the production of hemoglobin. The mutated gene changes the CAG codon into UAG in the mRNA. Explain how this will affect the protein produced. 8. The mapping of the human genome has the potential to be one of the most beneficial discoveries in science. How will the mapping of human chromosomes be beneficial in the treatment of genetic diseases? 9. Escherischia coli is a bacterium which lives in the human digestive system. Analysis of its DNA reveals that 21% of the nucleotides contain thymine. What percentage of nucleotides contain each of the other nitrogen bases? 10. The following sequence of bases is found in a small portion of DNA ATTACGGACT a) What is the base sequence in the replicated strand of this molecule? b) What molecules are found in the backbone of each strand? 11. Explain why the manner in which DNA is replicated is called semiconservative replication. 12. Human growth hormone causes growth in muscles, bones and connective tissue. Geneticists have inserted the gene for human growth hormone into mouse embryos and recorded the growth in these mice to be twice as large as mice without the added gene. Describe the procedure that is involved in carrying out this genetic technology. 13. What is the aim of the human genome project? 14. Identify the controversial side of the human genome project. BIO 30 CH 18 MOLECULAR GENETICS REVIEW Answer Section MULTIPLE CHOICE 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. ANS: ANS: ANS: ANS: ANS: ANS: ANS: ANS: ANS: ANS: ANS: ANS: ANS: ANS: ANS: ANS: ANS: ANS: ANS: ANS: ANS: ANS: ANS: ANS: ANS: ANS: ANS: ANS: ANS: ANS: ANS: ANS: ANS: ANS: ANS: C D A B C B A A B B C C B A D C B C C B D D C A A B A A A C D B C C A COMPLETION 1. 2. 3. 4. 5. 6. 7. ANS: ANS: ANS: ANS: ANS: ANS: ANS: MATCHING uracil mRNA amino acids TGTTAC AUG termination tryptophan, isoleucine 8. ANS: exons, introns 9. ANS: tRNA molecule 10. ANS: frameshift 1. ANS: 5. ANS: SHORT ANSWER C G C G C H 2. ANS: C 6. ANS: E C G C G Pro A T A U T A U A 3. ANS: D 7. ANS: F G C G C Trp G C G C A T U A C G C G Ser 4. ANS: B 8. ANS: G T A U A DNA mRNA tRNA amino acids 2. tRNA - transfer RNA. A molecule of RNA that transports amino acids to the ribosome where the protein is being built. mRNA - messenger RNA. A transcribed strand of RNA that is translated into protein by ribosomes. rRNA - ribosomal RNA. Binds with proteins to form ribosomes. 3. Transcription - conversion of DNA into mRNA Translation - conversion of mRNA into amino acid sequence. 4. a) AUG GGU/GGC/GGA/GGG CCU/CCC/CCA/CCG GUU/GUC/GUA/GAG AGA/AGG/CGU/CGC/CGA/CGG b) More than 1 sequence is possible since some amino acids are coded for by more than 1 codon. c) Variability in mRNA due to mutation can still produce the same amino acid sequence since some amino acids are coded for by up to 6 different codons. 5. 1. DNA replication produces two double stranded molecules. Transcription produces a single stranded molecule. 2. DNA replication copies the entire DNA code. Transcription makes a short section of the DNA. 3. DNA nucleotides include thymines, while RNA contains uracil 6. If mRNA can not be produced, proteins can not be synthesized. Functional proteins serve a variety of essential body functions and include hormones and enzymes which the body needs in order to maintain homeostasis. 7. Changing the codon CAG to UAG causes the amino acid glutamine to a stop codon. This will result in the termination of the amino acid sequence resulting in a protein being produced that does not serve the required function. 8. Once the location of a specific gene that causes a disorder is known, a number of treatments can be used. Gene therapy is possible in certain situations. The development of antibiotics, or other therapies is also possible since the code for the development of the protein that causes the disease will be known. 9. 29% cytosine 29% guanine 21% adenine 10. a) TAATGCCTGA b) deoxyribose sugar and phosphate 11. Semiconservative replication refers to the production of two strands of identical DNA sequences that each have one of the original DNA strands. 12. Isolate the gene for human growth hormone. Remove the gene using restriction enzymes. Insert the gene into a plasmid using ligase and restriction enzymes. Insert the plasmid into the first cell of the mouse embryo. Implant the embryo into the oviduct of a female mouse. 13. The aim of the project is to gain an understanding of the DNA sequencing in humans in order to develop new drugs and genetic therapies that combat genetic disorders. 14. The project's controversial side involves the ethical questions, legal dilemmas, and societal problems that will surround how the information will be used and who will have access to it.