Download Model question Paper- Gene Technology MLAB 475

Kingdom of Saudi Arabia
Ministry of Education
Prince Sattam Bin Abdulaziz University
College of Applied Medical Science in
Wadi Addawasir
‫المملكة العربية السعودية‬
‫وزارة التعليم‬
‫جامعة األمير سطام بن عبدالعزيز‬
‫كلية العلوم الطبية التطبيقية بوادي الدواسر‬
‫هـ‬1438 /1437 ‫ )المستوى السابع( للعام الجامعي‬- ‫اختبار الفصل الدراسي األول‬
Final Exam – 1st Semester (7th Level) For the Academic Year 1437/1438H
----------------------------------------------------------------------------------Subject: Gene technology and Molecular techniques (MLAB 475)
Student Name: ------------------------------------------
Time: 2 hours
Student Number: ----------------------------------------
Marks: 40 Marks
Answer the following questions:
Question 1: Choose the correct answer of the following
(5x1=5 marks)
1. The most common type of gel used for DNA separation is _________
(a) agar
(b) polyacrylamide
(c) agarose
(d) all of the above
2. Which of the following technique is suitable for identifying mRNA molecule in a
sample?
(a) Dot blotting
(b) Western blotting
(c) Southern blotting
(d) Northern blotting
Dr. Ghazala Muteeb
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3. Which of the following technique is most suitable for detecting the presence of a
gene product?
(a) Dot blotting
(b) Western blotting
(c) Southern blotting
(d) Northern blotting
4. Which of the following terms best describes the procedure in which DNA
fragments are separated on an agarose gel, transferred onto a nylon
membrane, and screened with a labeled hybridization probe?
(a) Dot blotting
(b) Southern blotting
(d) Northern blotting
(e) Western blotting
5. For the agarose gel result shown below, what is the approximate length of
DNA band in lane 3?
(a) 500 bp
(b) 70 bp
(c) 130 bp
(d) 1000 bp
Dr. Ghazala Muteeb
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Question 2: State True(T) or False(F) for the following statements.
(9x1=9 marks)
S. No.
Statement
Answer
1.
Pharming is a recent and controversial innovation in gene
cloning.
2.
Protein is responsible for preserving, copying and transmitting
information within cells and from generation to generation.
3.
Molecular diagnostics methods study primary structure of
DNA.
4.
Ribotyping has both taxonomic and epidemiological value.
5.
Gene is the basic unit of heredity.
6.
If a cloned gene is attached to the promoter for the sheep’s βlactoglobulin gene, the recombinant protein is secreted in the
urine.
7.
Plants offer an expensive means of mass production of
recombinant proteins.
8.
DNA probes allow for the diagnosis of infections in which the
organisms are not easily cultured or cannot be cultured at all.
9.
Mature red blood cells have the normal complement
of chromosomes.
Dr. Ghazala Muteeb
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Question-3: Match the names of enzymes mentioned in column A with their
(10x½=5 marks)
role in column B
Answer
Column A
1. DNA ligase
Column B
A. can digest cell walls enzymatically.
2. DNA polymerase B. binds to and stabilizes the unpaired DNA
strands.
3. Topoisomerase
C. untwists the two parallel DNA strands.
4. Helicase
D. can join Okazaki fragments to one another.
5. Single strand
binding protein
E. catalyzes the elongation of DNA by
adding nucleoside triphosphates to the 5’
end of the growing strand.
6. Primase is
F. degrades DNA into smaller components.
7. Horseradish
peroxidase
G. degrades luminol with the emission of
chemiluminescence.
8. Lysozyme
H. catalyzes the elongation of DNA by adding
nucleoside triphosphates to the 3’end of the
growing strand.
9. RNase
I. relieves the stress of twisting DNA strands.
10. DNase
J. degrades RNA into smaller components.
K. the enzyme in humans that can start an RNA
chain from scratch.
Dr. Ghazala Muteeb
Page 4 out of 9
Question 4:
(3x1=3 marks)
a) The restriction enzyme EcoRI recognizes a 6 base pair, “palindromic”
sequence in double stranded DNA. The first three bases of one strand are given;
complete the restriction site for EcoRI.
(2 marks)
5´ G A A _ _ _ 3´
3´ _ _ _ _ _ _
5´
b) EcoRI cuts both strands of DNA. The position of the first cut is indicated by
the arrow above. Draw an arrow to indicate the position of the second cut.
(1 mark)
Dr. Ghazala Muteeb
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Question 5:
(9 marks)
A) Write a program for thermo cycler to perform a simple Polymerase Chain
Reaction (PCR). Mention the temperature and time duration of each step.
(6 x½= 3 marks)
B) If the DNA is subjected to 6 cycles of Polymerase Chain Reaction (PCR),
how many copies of DNA will you get? Write the general formula that you
used to calculate the answer.
Dr. Ghazala Muteeb
(2 marks)
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C) A gene is cloned in the MCS of lacZ’ of E. coli. The transformants are
selected by blue white selection- lacZ’ complementation on X-gal plate and two
kinds of colonies are observed. Explain the significance of blue and white
colored colonies.
Dr. Ghazala Muteeb
(4 marks)
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Question 6: In 1962 Watson, Crick and Wilkins won the Nobel Prize for their
discovery of the structure of DNA. The figure below shows a picture that was
shown to Watson by Wilkins in early 1953. Answer the following with respect to
this picture:
(3 marks)
1) Name the scientist whose experimental work was directly responsible for the
pattern shown in this picture.
(1 mark)
………………………………………..
2) Which experimental method was used to generate this picture?
(1 mark)
…………………………………………
3) Name two important postulates of Watson and Crick model of DNA that
were actually calculated from this picture.
(1 mark)
…………………………………………
…………………………………………
Dr. Ghazala Muteeb
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Question 7: Make a flowchart to outline any one (01) of the following:
(6x1=6 marks)
A) The general steps involved in extracting DNA from cells.
OR
B) The general steps involved in colony hybridization probing.
The End
Good Luck
Dr. Ghazala Muteeb
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