Download 7 Self study questions

7 Self study questions
All Sections of Chapter 7
Fig 6,7,8,13,14,19,20,22,25,26
1. Explain why ORF scanning is a feasible way of identifying genes in a prokaryotic DNA sequence.
2. What modifications are introduced when ORF scanning is applied to a eukaryotic DNA sequence?
3. Describe how homology searching is used to locate genes in a DNA sequence and to assign possible functions to
those genes.
4. Distinguish between northern blotting and zoo-blotting. What are the applications of these two techniques in
gene location?
5. Explain how cDNA capture or cDNA selection are used to enrich a clone library for a particular cDNA sequence.
6. Draw a fully annotated diagram illustrating the procedure called 5 -RACE.
7. Describe how S1 nuclease is used to map the positions of the ends of a transcript on to a DNA sequence.
8. What experimental methods can be used to locate exon-intron boundaries in a DNA sequence?
9. Using the yeast genome project as an example, illustrate the strengths and weaknesses of homology analysis as
a means of assigning functions to unknown genes.
10. Describe how gene inactivation can be used to determine the function of an unknown gene.
11. Give an example of the use of gene overexpression to determine the function of an unknown gene.
12. Describe how oligonucleotide-directed mutagenesis is carried out and outline the use of this technique in
studying the activity of the protein coded by an unknown gene.
13. What is a reporter gene and how is it used?
14. Describe the methods used to study transcriptomes.
15. Explain how two-dimensional gel electrophoresis combined with mass spectrometry is used to study a proteome.
16. Draw diagrams illustrating the techniques called (a) phage display, and (b) the yeast two-hybrid system. What
are the similarities and differences between these two techniques?
17. What is a protein interaction map? What has the yeast protein interaction map told us about the construction
of the proteome of this organism?
18. Define the term 'synteny' and, using examples, explain how synteny can predict the positions of genes in a
genome sequence.
19. Describe the applications of comparative genomics in the study of human disease genes.