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Typical Animal Cell Blood cell from the insect Agallia constricta showing showing typical cellular organelles The large central nucleus (N) is surrounded by a dense cytoplasm containing endoplasmic reticulum (ER), ribosomes (R), mitochondria (M), and a golgi apparatus (GA) Magnification 5 650X Nucleus Nucleus from an insect (Estigmene acrea) nerve cell. Note the dense nucleolus (Nu), and the chromatin dispersed through the nucleus but with some local aggregations. A double envelop (NE) surrounds the nucleus. Magnification 5 100X Nucleolus Nucleolus from a salt marsh caterpillar (Estigmene acrea) fat body cell. The nucleolus (Nu) consists of an extremely fine-textured matrix material within which are embedded granules (arrows) about 15 nanometres in diameter. Magnification 27 500X Nuclear Envelope Nuclei are surrounded by a double membrane known as a nuclear envelope (NE). The envelope is penetrated by large nuclear pores (arrows), 100 nanometres in diametre. Magnification 27 500X Nuclear Pores Tangential section through the nuclear envelope of an insect (Estigmene acrea) intestinal cell. Note circular nuclear pores (arrows) approximately 100 nanometres in diametre. Magnification 8 400X Endoplasmic Reticulum Section through an intestinal cell of Estigmene acrea showing the cytoplasm packed with rough surfaced endoplasmic reticulum (RER) Magnification 8 400X Glycogen Glycogen (Gy) in animal cells is usually in the form of conspicuous rosettes of varying size. The rosettes are composed of isodiametric, 15 – 30 nanometre particles. From the insect Estigmene acrea. Magnification 8 400X Ribosomes Ribosomes isolated from the fat body cells of the gypsy moth Porthetria dispar. Single (A) ribosomes and clusters (B) of ribosomes (polyribosomes) are seen at high magnification. Each ribosome is approximately 22 nanometres in diametre. Magnification 60 000X Golgi Apparatus The golgi apparatus (GA) consists typically of a series of double membranes concentrically bent. Along the periphery of the membranes, small round golgi vesicles (GV) ligate. From the insect, Estigmene acrea. Magnification 27 400X Mitochondrion A mitochondrion from the insect Agallia constricta. The mitochondrion is surrounded by a double envelope consisting of an inner and an outer membrane (arrows). The inner mitochondrial membrane invaginates to form cristae (C). Magnification 27 500X Microvilli Fine structure of the apical border of a mid-gut epithelial cell from the insect E. acrea. Apical cell membrane (arrows) is extended at regular intervals along microvilli (Mv). Note fine fibrils (F) oriented along the length of the microvilli Magnification 27 500X Muscle Fibres The striped flight muscles of the mosquito (Culex pipiens) cut longitudinally. The light I band is bisected by the Z line where the actin filaments are joined to one another laterally. Magnification 9 000X Desmosome The fine structure of the intercellular junction in the mid-gut of the insect Estigmene acrea. The apposed lateral cell membranes are linked together by the regular array of transverse structures characteristic of septate desmosomes (SD). Magnification 27 400X Mitotic Cell Mitosis in a culture cell of the insect Agallia constricta. Chromosomes (Ch) are aligned in a metaphase plate. Note several spindle fibres (SF) which appear to be attached to the chromosomes. Magnification 4 800X Chromosomes Part of a metaphase plate showing the rather indefinite outline of the chromosome (Ch) and the tubular spindle fibres (SF) some of which appear to be attached to the chromosomes. From the embryonic cells of the insect Agallia constricta. Magnification 8 000X Nerve Axons Section through a central ganglion of the insect E. acrea. Many axons (Ax), each containing many neurotubules (Nt) and mitochondria (M) are seen in transverse section. Magnification 9 000X Pinocytotic Vesicles Mid-gut epithelial cell from the insect Estigmene acrea. Between the microvilli, small pits are formed which pinch off into the cell as pinocytotic vesicles (arrows and PV) Note the endoplasmic reticulum with ribosomes attached to it. Magnification 27 400X Lysosomes Lysosomes (Ly) and multivesicular bodies (MVE) in an intestinal cell of the insect Agallia constricta. Both organelles are membrane-bound and contain a number of hydrolytic enzymes. Magnification 27 400X Lipid Inclusions Part of a fat body from the insect Agallia constricta. Large lipid droplets (L) and many clusters of glycogen granules (Gy) are present. Magnification 4 000X Basement Membrane The basement membrane (BM) follows the contour of the base of a mid-gut epithelia cell from the insect Estigmene acrea larva. Note the irregular enfolding of the plasma (PM) and the many mitochondria which lie in the resulting narrow cytoplasmic sheets. Magnification 8 100X THE END