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Review Questions for Modern Genetics Match the description with the term or phrase. 1. adenine C 2. phosphorus C 3. thymine A 4. uracil B 5. purines C 6. pyrimidines C 7. phosphodiester bonds C 8. deoxyribose A B 9. ribose C 10. 5 carbon sugar 11. glucose D 12. nucleotides C A. In DNA and not RNA B. In RNA and not DNA C. In both DNA and RNA D. In neither DNA nor RNA Match the description with the term or phrase. 1. Poly-A tail B 2. TATA box A 3. Binding site(s) for transcription factors A 4. Binding site for amino acid B 5. enhancer A 6. activator D 7. Binding site for repressor A 8. Promoter A 9. Found in nucleus C 10. Found in cytoplasm B A. DNA and not RNA B. RNA and not DNA C. both DNA and RNA D. neither DNA nor RNA Put the descriptions where they belong Transcription Occurs in the nucleus Produces RNA Translation RNA polymerase Begins with the binding of the promoter Occurs in the cytoplasm Produces polypeptide Uses energy Uses a DNA template Produces water Occurs in cytosol of prokaryotes Is followed by RNA processing in RNA template Uses enzymes Put the terms where they belong Prokaryote 5’ GTP cap nucleus Poly-A tail Removes introns transcription Splices exons translation Free ribosomes Eukaryote Ribosomes on ER Histone proteins One kind of RNA polymerase Linear chromosomes Circular 3 kinds of RNA polymerase chromosome Put the following in order of their use during DNA replication. • DNA polymerase • Primase (an RNA polymerase) • Ligase • SSBP’s • Helicase 1. Helicase 2. SSBP’s 3. Primase (an RNA polymerase) 4. DNA polymerase 5. Ligase True or False: If the statement is false, replace the underlined word/phrase to make the statement read true. 1. The main enzyme involved in DNA replication is RNA polymerase. FALSE – DNA polymerase. 2. To determine the amino acid, look up the three base anticodon on the genetic dictionary FALSE – codon. 3. Ligase joins DNA fragments of the lagging strand. TRUE 4. DNA polymerase lengthens the new strands from 3’ to 5’. FALSE – 5’ to 3’ 5. The enzyme telomerase shortens the telomeres with each replication. FALSE – lengthens 6. Primers are made of short segments of DNA. FALSE – RNA 7. Adenine, Guanine, Thymine, Cytosine and Uracil are examples of nucleic acids. FALSE – nitrogenous bases Which of the following does not belong? Write one or two sentences using the other words that explain how all the other words are related. 1. DNA polymerase, aminoacyl-tRNA synthetase, ligase, helicase, primase Aminoacyl-tRNA synthetase joins amino acids to tRNA during translation, not replication. During replication, helicase unwinds and separates DNA strands, primase creates the RNA primers, DNA polymerase extends the new DNA strands, and ligase joins Okazaki fragments. 2. ribosome, tRNA, methionine, initiation, mRNA, stop codon, primer Primer is needed to begin DNA replication. During translation, mRNA binds between the two subunits of the ribosome, tRNA carries the first amino acid methionine to the ribosome during the initiation phase of translation and the stop codon signals termination of translation.