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Download Bio 313 worksheet 14 - Iowa State University
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BIO 313 SI WORKSHEET 14 Supplemental Instruction Iowa State University Leader: Course: Instructor: Date: For the following state whether it is a characteristic of Prokaryotes, Eukaryotes, or both 1. Transcription occurs in the nucleus and translation in the cytoplasm 2. Able to utilize post-transcriptional control 3. Transcription unit contains promoter, RNA coding region, and terminator 4. Transcription and translation are coupled events 5. Contain introns that are spliced out 6. Mature RNA has a polyA tail and 5’ cap 7. RNA is often polycistronic 8. Possesses colinearity 9. May contain a Rho factor 10. Undergoes splicing during mRNA processing 11. What is an operon? 12. The process of capping, polyadenylation, and splicing is collectively termed what? 13. What is capping? Explain what happens and where at on the RNA molecule 14. Why does capping occur? 15. What is polyadenylation? 16. Write out of the polyA consensus sequence 17. What is the purpose of the polyA tail? (2 things) 18. Where is the 3’ cleavage site in relation to the polyA consensus sequence? Use upstream/downstream and say approximately how many bases 1060 Hixson-Lied Student Success Center 515-294-6624 [email protected] http://www.si.iastate.edu 19. Diagram 3’ cleavage and polyadenylation. Include the consensus sequence. Do this in 4 steps with the first step being the DNA molecule. Second step being the primary transcript 20. What is the purpose of pre-mRNA splicing 21. What ribonucleoprotein carries out splicing? 22. What part of the cell does splicing take place? 23. (#27 from book) What would be the most likely effect of a mutation at the following locations in an E. coli gene? a. -8 b. -35 c. -20 d. Start site of transcription 24. (#30 from book) Suppose that a mutation occurs in the middle of a large intron of a gene encoding a protein. What will the most likely effect of the mutation be on the amino acid sequence of that protein? Explain