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Microbiology
Microbiology

... Physiological benefits of carnitine for bacteria Animals have been the focus of research on carnitine since its discovery in 1905 (Gulewitsch & Krimberg, 1905) and microbial carnitine metabolism was not described until more than 50 years later (Fraenkel & Friedman, 1957). In the intervening years, m ...
Carnitine metabolism and biosynthesis in yeast Saccharomyces
Carnitine metabolism and biosynthesis in yeast Saccharomyces

... acyl residues between intracellular compartments. This function of carnitine, referred to as the carnitine shuttle, is supported by the activities of carnitine acyltransferases and carnitine/acylcarnitine transporters, and is reasonably well studied and understood. While this function remains the on ...
procite - UWI St. Augustine
procite - UWI St. Augustine

... - Jim Apostolopoulos is the author of the PDOC00699 entry. - Brigitte Boeckmann is the author of the PDOC00691, PDOC00703, PDOC00829, PDOC00796, PDOC00798, PDOC00799, PDOC00906, PDOC00907, PDOC00908, PDOC00912, PDOC00913, PDOC00924, PDOC00928, PDOC00929, PDOC00955, PDOC00961, PDOC00966, PDOC00988 an ...
fiber types in mammalian skeletal muscles
fiber types in mammalian skeletal muscles

... of fast-twitch muscles, characterized by glycolytic metabolism and specialized for phasic activity, generally identified with white muscles, and slow-twitch muscles, rich in myoglobin and oxidative enzymes and specialized for more continuous activity, also called red muscles (562). The mechanical an ...
Identification and first characterization of pairing
Identification and first characterization of pairing

... Furthermore, it has been shown, that females induce lipid accumulation and lipase activity in males (Haseeb et al., 1989). However, research on a factor transferred from the female to the male was neglected as it was assumed to be uncertain and less promising concerning schistosomiasis control compa ...
Bettendorff L, Wins P. Biological functions of thiamine
Bettendorff L, Wins P. Biological functions of thiamine

... suggested that this selective vulnerability could be due to a coenzyme-independent role of thiamine or one of its derivatives4. Indeed, in addition to ThDP and free thiamine, several other phosphorylated and adenylated derivatives are observed (Fig. 2): thiamine monophosphate (ThMP), thiamine tripho ...
Functional Interactions Between the Subunits of the Lactose
Functional Interactions Between the Subunits of the Lactose

... detergent type or concentration. The membrane-embedded oligomeric state of XylP and LacS has been determined using freeze-fracture electron microscopy, which suggested that both proteins are present in the bilayer as dimers only (32,123). Additional evidence for this dimeric state of LacS in the mem ...
43803_C000 1..20
43803_C000 1..20

... Printed in the United States of America on acid-free paper ...
Identification and Cloning of a Cryptococcal Deacetylase That
Identification and Cloning of a Cryptococcal Deacetylase That

... to strain JEC 21, which was chosen for sequencing by thè Stanford University Technology Center C. neoformans Genome Project. The highly virulent serotype A C. neoformans strain H99 was used to establish a mode) of experimental cryptococcosis. Both C. neoformans H99 (ATCC 208821) and Blastomyces derm ...
Regulation of mitochondrial calcium in plants versus animals
Regulation of mitochondrial calcium in plants versus animals

... members that share >40% sequence identity (Fig. 2B). MICU1, the first uniporter component identified (Perocchi et  al., 2010), is a 50 kDa protein with two functional and two pseudo EF-hands and resides in the mitochondrial IMS (Csordás et al., 2013; Hung et al., 2014; Patron et al., 2014; Wang et a ...
Glutamine metabolism and its effects on immune response
Glutamine metabolism and its effects on immune response

... severe sepsis definition includes, among other factors, the presence of two or more criteria for SIRS; however, a recent study addressed cases of individuals with SIRSnegative severe sepsis [53]. Moreover, recently, the definition of sepsis was revised, the new definition consist in a systemic respo ...
ASD v2.0: updated content and novel features
ASD v2.0: updated content and novel features

... create plenty of opportunities to recognize novel allosteric molecules, leading to an explosive growth in the number of allosteric proteins and allosteric modulators. Intrinsically, the allosteric regulation of proteins is triggered by the binding of a modulator to their allosteric sites; thus, it i ...
Auxin: Regulation, Action, and Interaction
Auxin: Regulation, Action, and Interaction

... pathways is fully elucidated. Plants can also obtain IAA by b-oxidation of indole-3-butyric acid (IBA), a second endogenous auxin, or by hydrolysing IAA conjugates, in which IAA is linked to amino acids, sugars or peptides. To permanently inactivate IAA, plants can employ conjugation and direct oxid ...
Complete genome sequence of the prototype lactic acid bacterium
Complete genome sequence of the prototype lactic acid bacterium

... used as a cell factory for a wide variety of heterologous products (e.g., antimicrobials, including bacteriocins [50], bacteriophage endolysins [75], and defensins [47]). A recent review provides a snapshot of this diversity involving the favored “NICE” system (for nisin-controlled protein overexpre ...
UNIVERSITAT ROVIRA I VIRGILI CODON USAGE ADAPTATION IN PROKARYOTIC GENOMES
UNIVERSITAT ROVIRA I VIRGILI CODON USAGE ADAPTATION IN PROKARYOTIC GENOMES

... widely accepted that mitochondrion had a single origin, arising from a bacterial symbiont whose closest contemporary relatives are found within the a-proteobacteria (33, 34). Since its origin, the mitochondrial genome has undergone a streamlining process of genome reduction with intense periods of l ...
Food/feed and environmental risk assessment of insect
Food/feed and environmental risk assessment of insect

... fitness, potential for gene transfer, interactions between the GM plant and target and non-target organisms, effects on biogeochemical processes. It is emphasized that the VKM mandate does not include assessments of contribution to sustainable development, societal utility and ethical considerations ...
Purification and Characterization of Chorismate
Purification and Characterization of Chorismate

... polypeptide, the arom complex. In higher plants, two activities (3-dehydroquinate dehydratase and shikimate dehydrogenase) form a bifunctional polypeptide, although the other five enzymes are monofunctional (reviewed in ref. 4). In Euglena gracilis, the organization of the prechorismate pathway appe ...
Osmo- and thermo-adaptation in hyperthermophilic Archaea
Osmo- and thermo-adaptation in hyperthermophilic Archaea

... isolated from a variety of hot environments, most species originate from marine geothermal areas, hence they are slightly halophilic. The accumulation of low-molecular mass organic solutes, i. e., compatible solutes, is one of the most common strategies developed by cells to cope with fluctuations o ...
Characterization of the Two-Component, FAD-Dependent Monooxygenase SgcC That Requires Carrier Protein-Tethered
Characterization of the Two-Component, FAD-Dependent Monooxygenase SgcC That Requires Carrier Protein-Tethered

... Abstract: C-1027 is a potent antitumor antibiotic composed of an apoprotein (CagA) and a reactive enediyne chromophore. The chromophore has four distinct chemical moieties, including an (S)-3-chloro-5-hydroxyβ-tyrosine moiety, the biosynthesis of which from L-R-tyrosine requires five proteins: SgcC, ...
DMD #620 1 Identification of di (2-ethylhexyl) phthalate
DMD #620 1 Identification of di (2-ethylhexyl) phthalate

... 1994), were raised in male Japanese white rabbits (2.5 – 3.0 kg in body weight) according to a previously described procedure (Hosokawa et al., 1987). Anti-CES RL1 antibodies and anti-CES D1 antibodies were prepared as previously described (Hosokawa et al., 1990; Hosokawa et al., 2001). The IgG frac ...
CHAPTER  1 INTRODUCTION
CHAPTER 1 INTRODUCTION

... arable land. If the World ' s popUlation continues to grow at the present rates to a projected 12 billion by 2025 , it is anticipated that more cultivated land will be needed. Because of the limited area of new arable land available, expanded food production will depend on increasing energy inputs. ...
Elucidating Flux Regulation of the Fermentation Modes of
Elucidating Flux Regulation of the Fermentation Modes of

... lactic acid bacterium, as one of the most extensively characterized low GC organisms. The relatively simple metabolism of L. lactis has also made it an attractive target for metabolic engineering for the production of non-food related chemicals. Moreover, the status of being the first genetically mo ...
PRODUCTION OF PYRUVATE AND LACTATE BY
PRODUCTION OF PYRUVATE AND LACTATE BY

... liquid phase and requires high pressure. Lactonitrile is recovered and purified by distillation and then hydrolyzed to lactic acid and the corresponding ammonium salt either by concentrated HCl or by H2SO4. For recovery and purify, this crude lactic acid is esterifed by methanol to produce methyl la ...
Xenopus Spinal Neurons Express Kv2 Potassium Channel
Xenopus Spinal Neurons Express Kv2 Potassium Channel

... of Chandy, 1991) have been identified in ...
please refer to anzfa`s guide to applications and proposals for a
please refer to anzfa`s guide to applications and proposals for a

... FSANZ invites public comment on this Report based on regulation impact principles for the purpose of preparing an amendment to the Code for approval by the FSANZ Board. Written submissions are invited from interested individuals and organisations to assist FSANZ in further considering this Applicati ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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