• Study Resource
  • Explore
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
yeast in food technology - Lilis Nuraida`s blog
yeast in food technology - Lilis Nuraida`s blog

... Southeast Asian Food and Agriculture Science & Technology Center and Departement of Food Science and Technology ...
A Truncated Laminin Chain Homologous to the B2 Chain: Structure
A Truncated Laminin Chain Homologous to the B2 Chain: Structure

... colocalization of these chains in basement membranes (58). In vitro de- and renaturation studies with EHS laminin have shown that the assembly of this laminin is a specific process and only molecules with one Ae chain, one Ble chain, and one B2e chain are formed (35, 36). The carboxyl-terminal parts ...
Pyruvate dehydrogenase and the regulation of glucose oxidation in
Pyruvate dehydrogenase and the regulation of glucose oxidation in

... glucose oxidation to glycolysis in hypertrophied hearts is due to reduced activity and / or expression of the pyruvate dehydrogenase complex (PDC). Methods: We examined the effects of dichloroacetate (DCA), an inhibitor of PDC kinase, and of alterations in exogenous palmitate supply on coupling of g ...
Functional characterization of the Mycobacterium
Functional characterization of the Mycobacterium

... gene product. The resulting ligation product, pMAL-c2X-pknJ, was transformed into E. coli BL21 Star (DE3) (Invitrogen) and the resulting recombinant protein was used for all assays described in this paper, except the PepChip assay. The DNA fragments encoding recombinant PepE and EmbR were generated ...
THE MULTIFARIOUS AND DYNAMIC REGULATION OF THE LIVING CELL Karen van Eunen
THE MULTIFARIOUS AND DYNAMIC REGULATION OF THE LIVING CELL Karen van Eunen

... can affect both others, the relation between them can be depicted as a triangle (Figure 1.3). For instance, signal transduction can either have a direct effect on metabolism or an indirect effect via gene expression. Since this thesis does not cover signal transduction, I will not discuss it any fur ...
C H A P
C H A P

... of the lipase(s) produced by this bacterium remain almost unknown, since only related enzymes such as H. pylori phospholipases A2 and C have been cloned and/or characterized (Weitkamp et al., 1993; Dorrell et al., 1999; General Introduction 3.4.2). Therefore, this chapter is focused on the isolation ...
Involvement of Polyamine Catabolism in the Regulation of Glucose
Involvement of Polyamine Catabolism in the Regulation of Glucose

... Polyamines (putrescine, spermidine and spermine) are ubiquitous low molecular weight amines that are positively charged under physiological conditions. Homeostatic control of intracellular polyamine levels is achieved by regulating the synthesis, catabolism and transport of these molecules. Spermidi ...
H. Heldt
H. Heldt

... attention has been given to the generation and utilization of transgenic plants. Since there are many excellent textbooks on general biochemistry, I have deliberately omitted dealing with elements such as the structure and function of amino acids, carbohydrates, and nucleotides; the function of nucl ...
Advanced Stochastic Protein Sequence Analysis
Advanced Stochastic Protein Sequence Analysis

... of various genes, predicted using bioinformatics methods, gave access to the understanding of at least parts of complex metabolic systems. Without such methods fundamental insights which are now widely accepted would not have been possible for years. Thus, the relevancy and the success of computatio ...
Plant Biochemistry
Plant Biochemistry

... attention has been given to the generation and utilization of transgenic plants. Since there are many excellent textbooks on general biochemistry, I have deliberately omitted dealing with elements such as the structure and function of amino acids, carbohydrates, and nucleotides; the function of nucl ...
Hydrolyzed Source Proteins as Used in Cosmetics
Hydrolyzed Source Proteins as Used in Cosmetics

... short fibers considered to be by-products of the textile industry. The noils are used in the production of hydrolyzed silk proteins through carefully controlled hydrolysis. The resultant material is a 5% solution of a water soluble silk protein. Hydrolyzed Serum Protein Hydrolyzed serum protein can ...
OMPROT PP2 ver4 - Plant Physiology
OMPROT PP2 ver4 - Plant Physiology

... proteins, such as OM64, which exhibits sequence similarity to a well characterized plastid localized protein, would be missed or incorrectly annotated, in an orthology based approach comparing mitochondrial proteins from other organisms (Chew et al., 2004). Using green fluorescent protein tagging (G ...
Purification and Characterization of
Purification and Characterization of

... holoenzyme structure and function, and thus may represent an alternative evolutionary pathway in Calvin-cycle kinase development. ...
Developement of strategies for the isotopic labeling of methyl
Developement of strategies for the isotopic labeling of methyl

... proposed pattern induces a 2-fold decrease of number of Leu and Val NMR signals and enhances the intensity of detectable long-range nOes by a factor 4. The described protocol also permits the suppression of spurious correlations, especially harmful for structural studies based on detection/analysis ...
How Optimized Is the Translational Machinery in
How Optimized Is the Translational Machinery in

... is almost perfect (the optimal is when Q M 5 1), suggesting that the effect of mutation on codon usage bias must be very weak for these genes. However, if we ignore the expressivity of the genes and pool the codon usage of all genes in the gene bank, then most Q M values are smaller than the p M val ...
Gene Section FST (follistatin)  Atlas of Genetics and Cytogenetics
Gene Section FST (follistatin) Atlas of Genetics and Cytogenetics

... bound activin is unable to initiate signal transduction and consequently follistatin is a potent antagonist of physiological activin signals. Of the three follistatin domains present in all follistatin isoforms, (Shimasaki et al., 1988) the first two, but not the third, are necessary for activin A b ...
- VU Research Repository
- VU Research Repository

... One of the major yeast stressors during fermentation is ethanol accumulation. Ethanol stress is associated with reduced cell growth and viability, consequently lowering yeast productivity. Although the underlying causes of ethanol inhibition of cells are yet to be identified, it has been discovered ...
thèse - Université Evry Val d`Essonne
thèse - Université Evry Val d`Essonne

... hypothesis of mitochondrial origin, popularized in the early seventies by Lynn Margulis’s book “the origin of eukaryotic cells”, stating that mitochondria evolved from a bacterial progenitor through symbiosis with an amitochondrial eukaryotic cell. These primitive eukaryotes were formally put in the ...
Eukaryote-Like Serine/Threonine Kinases and Phosphatases in
Eukaryote-Like Serine/Threonine Kinases and Phosphatases in

... on bacterial kinases with catalytic domains that share structural and functional homology with eukaryotic Ser/Thr kinases. These kinases are referred to herein as eSTKs to distinguish them from other prokaryotic enzymes that can also phosphorylate Ser/Thr residues (see the next section). Although ou ...
Suberin biosynthesis in O. sativa: characterisation of a cytochrome
Suberin biosynthesis in O. sativa: characterisation of a cytochrome

... An organism is defined to be alive when it features certain characteristics: It has to have the ability to reproduce itself, a metabolism to absorb and convert energy from the environment and to build its own components, it has to be discrete from its surrounding media, and maintain its homoeostasis ...
Major roles of isocitrate lyase and malate synthase in
Major roles of isocitrate lyase and malate synthase in

... inoculated onto wheat seedlings and leaves were unable to induce necrotic lesions on either tissue, indicating that this gene is essential for virulence on wheat. The biological reason for this phenotype is that the mls1-deficient strain has dramatically decreased spore germination and a reduction i ...
Insights into interactions between poly(ethylene glycol) and proteins
Insights into interactions between poly(ethylene glycol) and proteins

... that occur within organisms from in vitro biochemistry studies, one aspect that is often overlooked is the environment in which these processes naturally occur. Conventional biochemical experiments usually employ a low concentration (less than 1 mg/ml) of a single protein; however, in vivo, proteins ...
Transcription factor Sp3 is silenced through - IMT
Transcription factor Sp3 is silenced through - IMT

... GST±Sp3WT and GST±Sp3BID) or a mutated IKEE sequence (HA/FLAG-tagged Sp3SD and GST±Sp3kee), as outlined schematically in Figure 2B and C. In the GST±Sp3BID protein, an N- and C-terminal truncated Sp3 fragment is fused to GST. The Sp3 part contains the second glutamine-rich activation domain (B domai ...
- Free Documents
- Free Documents

... into proinsulin. Proinsulin is Iurther cleaved yielding active insulin which is composed oI two peptide chains linked togehter through disulIide bonds. Still other proteins oI the enzyme class are synthesized as inactive precursors called zymogens. Zymogens are activated by proteolytic cleavage such ...
Protein O-linked β-N-acetylglucosamine
Protein O-linked β-N-acetylglucosamine

... addition to glucose. The importance of the HBP and UDP-GlcNAc synthesis for cell viability is also emphasized by the fact that gene deletion of either EMeg32 or Agm1 is embryonically lethal [67,68]; surprisingly however, remarkably little is known about the regulation of HBP flux in the heart or inde ...
< 1 2 3 4 5 6 7 8 9 ... 221 >

Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report