Metabolism of acyl‐lipids in Chlamydomonas reinhardtii
... et al., 2012; Nguyen et al., 2013; Pflaster et al., 2014), and the composition and biosynthesis of FA in this organism was determined initially and investigated experimentally in the late 1980s (Giroud and Eichenberger, 1988; Giroud et al., 1988). Chlamydomonas FA compositions share features typical ...
... et al., 2012; Nguyen et al., 2013; Pflaster et al., 2014), and the composition and biosynthesis of FA in this organism was determined initially and investigated experimentally in the late 1980s (Giroud and Eichenberger, 1988; Giroud et al., 1988). Chlamydomonas FA compositions share features typical ...
BIOGENESIS OF MITOCHONDRIAL ATPase Studies on
... binding of F 1 to mitochondrial membrane and thus is one of the components which are essential for cold-stable and oligomycin-sensitive ATPase. The activity of OSCP increases during glucose derepression two to three fold as shown by reconstitution experiments with OSCP-enriched fractions [11]. The e ...
... binding of F 1 to mitochondrial membrane and thus is one of the components which are essential for cold-stable and oligomycin-sensitive ATPase. The activity of OSCP increases during glucose derepression two to three fold as shown by reconstitution experiments with OSCP-enriched fractions [11]. The e ...
PROTEIN PHOSPHORYLATION AND CELLULAR REGULATION, I by
... since we both had problems that we considered as our major areas of concentration, to pool our efforts to see whether or not we could obtain information on this point. The initial experiment that we undertook together was simply to prepare pure crystalline phosphorylase a by the Coris’ procedure (7) ...
... since we both had problems that we considered as our major areas of concentration, to pool our efforts to see whether or not we could obtain information on this point. The initial experiment that we undertook together was simply to prepare pure crystalline phosphorylase a by the Coris’ procedure (7) ...
Further studies on the new coomassie brilliant blue G-250 - K-REx
... The Lowry (Chou and Goldstein, 1960; Lowry et al, 1951; Harrow et al, 1962) which uses the Folin-Ciocalteu reagent, is currently the most sensitive ...
... The Lowry (Chou and Goldstein, 1960; Lowry et al, 1951; Harrow et al, 1962) which uses the Folin-Ciocalteu reagent, is currently the most sensitive ...
unexpected consequences for sense codon reassignment
... Modification of tRNA is widespread and essential for the proper functioning of the translational apparatus in all kingdoms of life. In Escherichia coli (E. coli), one of the three model systems for which the full extent of modifications has been mapped, the 46 tRNA species contain an average of 7.5 ...
... Modification of tRNA is widespread and essential for the proper functioning of the translational apparatus in all kingdoms of life. In Escherichia coli (E. coli), one of the three model systems for which the full extent of modifications has been mapped, the 46 tRNA species contain an average of 7.5 ...
Characterization of the Phosphoenolpyruvate Carboxykinase Gene
... pEK-pckA was isolated, labelled and hybridized to PvuIIrestricted and size-fractionated chromosomal DNA from C. glutamicum. The hybridization resulted in a single signal at 4.3 kb (not shown). This result confirms that the isolated DNA fragment corresponds to a fragment within the genome of C. gluta ...
... pEK-pckA was isolated, labelled and hybridized to PvuIIrestricted and size-fractionated chromosomal DNA from C. glutamicum. The hybridization resulted in a single signal at 4.3 kb (not shown). This result confirms that the isolated DNA fragment corresponds to a fragment within the genome of C. gluta ...
Appendix 1: PBS listings of Nutritional Products
... purposes and infant formula products are regulated by the Australia New Zealand Food Standards Code. Foods for special medical purposes are regulated by Standard 2.9.5 of the Food Standards Code. Infant formula products are regulated by Standard 2.9.1 of the Code even where they might meet the requi ...
... purposes and infant formula products are regulated by the Australia New Zealand Food Standards Code. Foods for special medical purposes are regulated by Standard 2.9.5 of the Food Standards Code. Infant formula products are regulated by Standard 2.9.1 of the Code even where they might meet the requi ...
REDESIGN OF CARNITINE ACETYLTRANSFERASE SPECIFICITY BY PROTEIN ENGINEERING UNIVERSIDAD DE BARCELONA
... the primary translation product has a 21 amino-acid sequence before the second methionine, which is the putative first amino acid in peroxisomal CrAT (CrAT per.). Thus, the peroxisomal isoform would consist of 605 amino acids. By comparison with other CrATs we postulated a 29-amino acid sequence tha ...
... the primary translation product has a 21 amino-acid sequence before the second methionine, which is the putative first amino acid in peroxisomal CrAT (CrAT per.). Thus, the peroxisomal isoform would consist of 605 amino acids. By comparison with other CrATs we postulated a 29-amino acid sequence tha ...
A proteomic study of African elephant milk: Inter
... further described as a functional food; that is, it contains health promoting compounds such as oligosaccharides, as well as proteins that impact on physiological functions (Casado et al., 2009). In essence, milk is an extremely complex fluid and comparative studies of bioactive, nutritional and pro ...
... further described as a functional food; that is, it contains health promoting compounds such as oligosaccharides, as well as proteins that impact on physiological functions (Casado et al., 2009). In essence, milk is an extremely complex fluid and comparative studies of bioactive, nutritional and pro ...
Isoelectric point prediction from the amino acid sequence of a protein
... 2.3 Extracting useful information from collected ...
... 2.3 Extracting useful information from collected ...
Potassium starvation responses in yeast highlight novel potassium-related functions
... uptake, intracellular traffic and efflux, and by a set of proteins regulating these processes. In spite of the importance of potassium for yeast physiology, not all relevant functional potassium-related targets have been identified. In this work, potassium starvation conditions are used to determine ...
... uptake, intracellular traffic and efflux, and by a set of proteins regulating these processes. In spite of the importance of potassium for yeast physiology, not all relevant functional potassium-related targets have been identified. In this work, potassium starvation conditions are used to determine ...
Analysis of the Arabidopsis rsr4-1/pdx1
... that constituents of the TCA cycle, such as malate, citrate, and fumarate, were highly upregulated (Figure 4B) and that shikimate, a key compound in indole-3-acetic acid (IAA) biosynthesis, was significantly reduced. Finally, relatively few changes in carbohydrate content were observed in the mutant ...
... that constituents of the TCA cycle, such as malate, citrate, and fumarate, were highly upregulated (Figure 4B) and that shikimate, a key compound in indole-3-acetic acid (IAA) biosynthesis, was significantly reduced. Finally, relatively few changes in carbohydrate content were observed in the mutant ...
5 Molecular basis of type-2 diabetes
... diabetes forms (MODY) or secondary beta-cell defects, caused by glucotoxicity, increased free fatty acids, cytokines and/or mitochondrial disfunction may be implicated in the physiopathology of type-2 diabetes. Classical physiology studies, biochemical methods, tissue culture technology, the use of ...
... diabetes forms (MODY) or secondary beta-cell defects, caused by glucotoxicity, increased free fatty acids, cytokines and/or mitochondrial disfunction may be implicated in the physiopathology of type-2 diabetes. Classical physiology studies, biochemical methods, tissue culture technology, the use of ...
Kinetic studies of human tyrosyl
... CACATGTTCCCATGC-3¢ were used as the forward and reverse primers. Similarly, oligonucleotides of 5¢-AAGTAT AACTCTCGAGCCCTCCACATCAAGG-3¢ and 5¢-GG AGGGCACCCACATGTTCCCATGC-3¢ were used as primers to generate the huTDPND174 variant. Generation of expression constructs to produce wildtype and mutant huma ...
... CACATGTTCCCATGC-3¢ were used as the forward and reverse primers. Similarly, oligonucleotides of 5¢-AAGTAT AACTCTCGAGCCCTCCACATCAAGG-3¢ and 5¢-GG AGGGCACCCACATGTTCCCATGC-3¢ were used as primers to generate the huTDPND174 variant. Generation of expression constructs to produce wildtype and mutant huma ...
Characteristics of Whey Flavor - Innovation Center for US Dairy
... mechanistic causes of astringency and consumer perception of astringency in high-protein acidic beverages. In one study conducted at University of Minnesota, researchers examined the overall acidity of whey protein beverages as a cause of astringency.26 Whey protein has a high-buffering capacity. Th ...
... mechanistic causes of astringency and consumer perception of astringency in high-protein acidic beverages. In one study conducted at University of Minnesota, researchers examined the overall acidity of whey protein beverages as a cause of astringency.26 Whey protein has a high-buffering capacity. Th ...
Molecular cloning of Per a 1 and definition of the cross
... associated with allergic diseases, especially asthma.1-5 Epidemiologic studies have shown a high prevalence of sensitization to cockroach allergens among lower socioeconomic groups living in cockroach-infested housing, and they have also shown that sensitization to cockroaches is a risk factor for h ...
... associated with allergic diseases, especially asthma.1-5 Epidemiologic studies have shown a high prevalence of sensitization to cockroach allergens among lower socioeconomic groups living in cockroach-infested housing, and they have also shown that sensitization to cockroaches is a risk factor for h ...
Molecular basis of hepatic carnitine palmitoyltransferase I deficiency
... Sequence analysis of these PCR fragments using fluorescent labeled M13 primers (Amersham) was performed on an Applied Biosystems 377A automated DNA sequencer following the manufacturer’s protocols. Construction of expression plasmids. Two expression plasmids were constructed for expression of CPTI-4 ...
... Sequence analysis of these PCR fragments using fluorescent labeled M13 primers (Amersham) was performed on an Applied Biosystems 377A automated DNA sequencer following the manufacturer’s protocols. Construction of expression plasmids. Two expression plasmids were constructed for expression of CPTI-4 ...
The Structure of the PanD/PanZ Protein Complex
... of enzymes have been shown to be enhanced by accessory proteins (McDonald et al., 1998), although the structural basis for such enhancement has not been determined. Since the first identification of pyruvoyl-dependent enzymes (van Poelje and Snell, 1990; Williamson and Brown, 1979), it has been wide ...
... of enzymes have been shown to be enhanced by accessory proteins (McDonald et al., 1998), although the structural basis for such enhancement has not been determined. Since the first identification of pyruvoyl-dependent enzymes (van Poelje and Snell, 1990; Williamson and Brown, 1979), it has been wide ...
Understanding the Regulation of Metabolic Enzyme Acetylation in E
... two forms: 1) Nα-acetylation, where an acetyl group from the acetyl donor (acetyl-CoA) is transferred to the amino terminus of a protein, or 2) Nε-acetylation, where an acetyl group is transferred to the ε-amino group of a lysine residue. Nα-acetylation is a nonreversible PTM that is rare in bacteri ...
... two forms: 1) Nα-acetylation, where an acetyl group from the acetyl donor (acetyl-CoA) is transferred to the amino terminus of a protein, or 2) Nε-acetylation, where an acetyl group is transferred to the ε-amino group of a lysine residue. Nα-acetylation is a nonreversible PTM that is rare in bacteri ...
SPT4, a gene important for tr
... Construction of spt4Al: :URA3. spt4 null mutants were constructed in several steps. First, Tn5 transposon mutagenesis was used to disrupt the SPT4 gene. Bacterial strain SY316 was transformed with pJF42. Ampicillinresistant transformants were purified on LB plates containing 50 gg/ml kanamycin, and ...
... Construction of spt4Al: :URA3. spt4 null mutants were constructed in several steps. First, Tn5 transposon mutagenesis was used to disrupt the SPT4 gene. Bacterial strain SY316 was transformed with pJF42. Ampicillinresistant transformants were purified on LB plates containing 50 gg/ml kanamycin, and ...
Modulation of the Oligomerization State of the Bovine F1
... Plasmids expressing IF1-H49K and IF1 truncated at the N terminus by 31 residues [IF1 (residues 32– 84)] were made from pRKIF1 by polymerase chain reaction using the oligonucleotide primers GAA GAA ACA CAA GGA AAA TGA GAT and TAG GAA TTC CAT ATG CGA TAC TTC CGA GCT CGT GCT AAA, respectively. The plas ...
... Plasmids expressing IF1-H49K and IF1 truncated at the N terminus by 31 residues [IF1 (residues 32– 84)] were made from pRKIF1 by polymerase chain reaction using the oligonucleotide primers GAA GAA ACA CAA GGA AAA TGA GAT and TAG GAA TTC CAT ATG CGA TAC TTC CGA GCT CGT GCT AAA, respectively. The plas ...
Zebrafish Collagen Type I: Molecular and Biochemical
... bone proteins favoured the understanding of late skeletogenesis events7,8. Furthermore, the TALEN and CRISPR/ Cas-based methods for genome editing developed in the last years allow the rapid generation of new zebrafish skeletal mutants9. Of relevance, since zebrafish are aquatic animals, the weight- ...
... bone proteins favoured the understanding of late skeletogenesis events7,8. Furthermore, the TALEN and CRISPR/ Cas-based methods for genome editing developed in the last years allow the rapid generation of new zebrafish skeletal mutants9. Of relevance, since zebrafish are aquatic animals, the weight- ...
Interactions of TCA cycle enzymes and of the CcpA
... carbon sources and NADP . Excess of α-ketoglutarate can be applied to amino acid anabolism, since αketoglutarate is the precursor molecule of glutamate. No interaction was detected between Mdh and CitZ by SPR even in the presence of the corresponding metabolites. This result was surprising, because ...
... carbon sources and NADP . Excess of α-ketoglutarate can be applied to amino acid anabolism, since αketoglutarate is the precursor molecule of glutamate. No interaction was detected between Mdh and CitZ by SPR even in the presence of the corresponding metabolites. This result was surprising, because ...
Identification of a structural motif that confers specific interaction with
... the Gal4 system. The amino acid-substituted COP1 proteins expressed as well as wild-type COP1 in both two-hybrid systems (data not shown). The use of different two-hybrid systems prevents a direct comparison between the HY5 and the STO/STH proteins; however, the relative effect that the WD40 domain ...
... the Gal4 system. The amino acid-substituted COP1 proteins expressed as well as wild-type COP1 in both two-hybrid systems (data not shown). The use of different two-hybrid systems prevents a direct comparison between the HY5 and the STO/STH proteins; however, the relative effect that the WD40 domain ...
Structure and Antioxidant Catalytic Function of Plant Glutathione Trans
... serves several important roles: (a) limit and restrict the reactivity of the chemicals; (b) increases their solubility and facilitates their membrane transport and elimination from the cell and organism; and (c) in some cases, it leads to the formation of secondary metabolites or essential biologica ...
... serves several important roles: (a) limit and restrict the reactivity of the chemicals; (b) increases their solubility and facilitates their membrane transport and elimination from the cell and organism; and (c) in some cases, it leads to the formation of secondary metabolites or essential biologica ...