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Angiotensin I-converting enzyme-inhibitory peptide fractions
Angiotensin I-converting enzyme-inhibitory peptide fractions

... All analyses throughout this research were performed in triplicate. Proximate analysis was carried out following the AOAC (2000) standard procedures; it included moisture, ash, crude protein, ether extract and crude fibre; carbohydrate concentration was calculated by difference. 2.3. Protein fraction ...
Full-Text PDF
Full-Text PDF

... carbon flux towards the product. It is uncertain why the first ethanol report using PCC 7942 was significantly less successful than using PCC 6803. However, it is possible that the gene expression was more stable in the PCC 6803 study as the two genes were introduced into the genome rather than usin ...
Characterization and the role of carbonic anhydrase
Characterization and the role of carbonic anhydrase

... simultaneous requirement that minimal carbonic anhydrase activity be found within the cystol. The CAs are ubiquitous metallo enzymes (mainly Zn) that catalyzes the quick reversible hydration reaction of CO2 to HCO3and protons (H+) or vice versa. This “reverse” reaction gives CA its name, because it ...
Identification of two amino acid residues which - Wiley-VCH
Identification of two amino acid residues which - Wiley-VCH

... a crystallized enzyme-substrate complex of the related enzyme chitobiase. The model contains a central hydrophilic region referred to as the “substrate cavity”. Figure 2 shows only this portion of the proposed structure. The three residues implicated in active site function cluster on the “left” sid ...
Probing the origins of glutathione biosynthesis through biochemical
Probing the origins of glutathione biosynthesis through biochemical

... multiple roles in maintaining cellular homoeostasis in response to a range of oxidative stresses [1]. As part of these protection systems, glutathione is critical for redox buffering, detoxification of xenobiotics, sulfur storage and transport, and as a modifier of protein function [1–6]. Photosynth ...
Cell and Molecular Biology
Cell and Molecular Biology

... Cell and Molecular Biology DNA Transcription ...
Functional characterisation of the TUP5 gene in - diss.fu
Functional characterisation of the TUP5 gene in - diss.fu

... Arginine is one of 20 standard amino acids necessary for the formation of peptides and proteins. It is also used as a nitrogen storage compound in seeds. Furthermore, arginine is the precursor of polyamines and nitric oxide which play an important role in many developmental processes and the respons ...
Insulin hormone: Mechanism and effects on the body and
Insulin hormone: Mechanism and effects on the body and

... 2 domains. IRS-1 in particular is phosphorylated on several tyrosine residues by the insulin receptor’s intracellular catalytic part and these phosphorylated tyrosine residues in turn employee other proteins with src homology 2 domains, one of which is phosphatidylinositol 3-kinase. Phosphatidylinos ...
Is transcriptomic regulation of berry development more
Is transcriptomic regulation of berry development more

... and anthocyanins accumulate [3] and major aromatic compounds including terpenes, norisoprenoids, esters and thiols are synthesized [10]. The control of these physiological processes is not well understood in the grapevine – which is a non-climacteric fruit exhibiting completely different development ...
ABA overlysensitive5 (ABO5), encoding a pentatricopeptide repeat
ABA overlysensitive5 (ABO5), encoding a pentatricopeptide repeat

... electron transport system. There are 30–40 NADH dehydrogenase subunits encoded by both mitochondrial and nuclear ...
Sucrose breakdown in the potato tuber - publish.UP
Sucrose breakdown in the potato tuber - publish.UP

... In this work different approaches are undertaken to improve the understanding of the sucroseto-starch pathway in developing potato tubers. At first an inducible gene expression system from fungal origin is optimised for the use of studying metabolism in the potato tuber. It is found that the alc sys ...
Matricellular Protein Cyr61 Bridges Lysophosphatidic Acid and
Matricellular Protein Cyr61 Bridges Lysophosphatidic Acid and

... removal of the cellular components was extracted by scraping at 90 °C in 1⫻ Laemmli sample buffer (60 mM Tris-HCl, pH 6.8, 2% SDS, 5% ␤-mercaptoethanol, 5% glycerol). These fractions were designated as ECM as described previously (20). Cell Migration Assay—Cell migration was performed by trypsinizin ...
Attachment 1 - Food Standards Australia New Zealand
Attachment 1 - Food Standards Australia New Zealand

... potential costs and benefits of the proposed change to the Code from stakeholders is highly desirable. Claims made in submissions should be supported wherever possible by referencing or including relevant studies, research findings, trials, surveys etc. Technical information should be in sufficient ...
Characterization of the Sucrose Phosphate Phosphatase (SPP
Characterization of the Sucrose Phosphate Phosphatase (SPP

... or rosette leaves, respectively. cDNA fragments were amplified by PCR using specific primers (Table 1) which were designed with the appropriate restriction sites and a start codon (Met). Next, they were cloned into the pGEM-T Easy vector and finally, in the desired expression vector, which incorpora ...
A PEST-like Sequence in the N-Terminal Cytoplasmic Domain of
A PEST-like Sequence in the N-Terminal Cytoplasmic Domain of

... ABSTRACT: Maltose permease is required for maltose transport into Saccharomyces cells. Glucose addition to maltose-fermenting cells causes selective delivery of this integral plasma membrane protein to the yeast vacuole via endocytosis for degradation by resident proteases. This glucose-induced degr ...
Proteomics of
Proteomics of

... strongly predominate over their monomeric forms in boar seminal plasma. These interactions facilitate the formation of aggregated forms of proteins in the seminal plasma and probably the arrangement and remodeling of sperm coating proteins. It is interesting that the heparin-binding activity of aggr ...
Plant Peroxisomes: Biogenesis and Function
Plant Peroxisomes: Biogenesis and Function

... only from the division of preexisting organelles (Figure 1B). The ER was thought to serve only as a source of membrane lipids for the enlargement of preexisting peroxisomes (e.g., via phospholipid transfer proteins and/or ER-peroxisome contact sites). Studies in yeasts and Chinese hamster ovary cell ...
Full-Text PDF
Full-Text PDF

... and ATP production were all examined in the presence of K2TeO3. Resistance was confirmed in RO liquid medium with varying concentrations of K2TeO3 (100, 250, 500, 750, 1000, and 1500 µg/mL). Growth was monitored spectrophotometrically at A950, an established method for estimating growth and reductio ...
Protein and Amino Acid Metabolism During and After Exercise
Protein and Amino Acid Metabolism During and After Exercise

... milieu. At rest, immediately after a mixed meal, the delivery of amino acids to muscle exceeds its capacity to deposit them as protein. The intramuscular amino acid pool is expanded (10) but not by as much as might be expected, because of protein synthesis, the inhibition of breakdown, and the stimu ...
METABOLIC AND ENDOCRINE ADAPTATIONS TO HEAT STRESS
METABOLIC AND ENDOCRINE ADAPTATIONS TO HEAT STRESS

... a lower tolerance to THI increase and they are more susceptible to heat stress than low producers. Suppressed feed intake is one of the markers in heat-stressed animals and has been blamed as the major culprit causing the devastating impact of heat stress on milk production in dairy industry (Beede ...
Role of adiponectin in the regulation of carbohydrate and lipid
Role of adiponectin in the regulation of carbohydrate and lipid

... (previously ...
Investigating the link between tRNA and mRNA - EMBL-EBI
Investigating the link between tRNA and mRNA - EMBL-EBI

... of protein-coding genes, which changes dynamically to drive cell function. The abundance of trna genes defines, to a large extent, the efficiency with which mrna can be translated into proteins. On the one hand, this serves to explain the need for the observed, stable trna abundance. On the other ha ...
The N-Terminal Region of Arabidopsis
The N-Terminal Region of Arabidopsis

... The expression of CGS constructs in vegetative tissues of the T0 plants was tested by western-blot analysis using anti-HA monoclonal antibodies. Figure 3 shows the results with three representative transgenic plants expressing relatively high levels of each of the Arabidopsis CGS constructs. As expe ...
VAAM-Jahrestagung 2015 1.–4. März in Marburg/Lahn
VAAM-Jahrestagung 2015 1.–4. März in Marburg/Lahn

... notorious Ebola virus. Its recent uncontrolled outbreak in Africa reminds us how vulnerable mankind is against infectious diseases that jump from animal reservoirs to humans. The Institute for Virology of the Philipps-University under the successive leadership of Hans-Dieter Klenk and Stephan Becker ...
Metabolic significance of inorganic triphosphate, thiamine
Metabolic significance of inorganic triphosphate, thiamine

... unusual triphosphate derivative of thiamine (vitamin B1) found in nearly all organisms. In mammalian tissues, ThTP is hydrolyzed by a very specific cytosolic thiamine triphosphatase (ThTPase) belonging to an ancient superfamily of proteins called CYTH. Several members of this superfamily have been c ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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