Functional and structural roles of parasite-specific inserts in the bifunctional S-adenosylmethionine decarboxylase/ornithine
... The collected size-exclusion chromatography fractions were transferred to nitrocellulose membranes using a BioDot apparatus (Bio-Rad) and analysed by dot blot Western. The membranes were blocked in 3% w/v low fat milk powder in lxPBS for 16 hours at 4°C followed by incubation with a 1:4000 dilution ...
... The collected size-exclusion chromatography fractions were transferred to nitrocellulose membranes using a BioDot apparatus (Bio-Rad) and analysed by dot blot Western. The membranes were blocked in 3% w/v low fat milk powder in lxPBS for 16 hours at 4°C followed by incubation with a 1:4000 dilution ...
Biochemistry 499
... Reversible phosphorylation of proteins is known to be a method of regulation of many cellular pathways. The enzymes involved in the reactions in reversible phosphorylation are protein kinases, which catalyze phosphorylation, and protein phosphatases, which catalyze dephosphorylation. Protein phospha ...
... Reversible phosphorylation of proteins is known to be a method of regulation of many cellular pathways. The enzymes involved in the reactions in reversible phosphorylation are protein kinases, which catalyze phosphorylation, and protein phosphatases, which catalyze dephosphorylation. Protein phospha ...
Proteome analysis of tobacco BY-2 cell culture - ETH E
... Plastids, essential cell organelles, are present in all living cells of plants, except pollen. They are responsible for many of the essential biosynthetic and metabolic activities required for the basic architecture and functions of plant cells. Depending on the tissue type, they are differentiated ...
... Plastids, essential cell organelles, are present in all living cells of plants, except pollen. They are responsible for many of the essential biosynthetic and metabolic activities required for the basic architecture and functions of plant cells. Depending on the tissue type, they are differentiated ...
Amino Acids, Peptides, and Proteins
... Nearly all biological compounds with a chiral center occur naturally in only one stereoisomeric form, either D or L. The amino acid residues in protein molecules are exclusively L stereoisomers. D-Amino acid residues have been found only in a few, generally small peptides, including some peptides of ...
... Nearly all biological compounds with a chiral center occur naturally in only one stereoisomeric form, either D or L. The amino acid residues in protein molecules are exclusively L stereoisomers. D-Amino acid residues have been found only in a few, generally small peptides, including some peptides of ...
Genetic engineering of lactic acid bacteria to produce
... pure L(+)-lactic acid. Naturally, this organism produces mixture of D(-)- and L(+)-lactic acid during fermentation of sugars. Production of D(-)- and L(+)-lactic acid is based on the activity of LdhD and LdhL, respectively. In this work, LdhD enzyme activity was removed by using two different geneti ...
... pure L(+)-lactic acid. Naturally, this organism produces mixture of D(-)- and L(+)-lactic acid during fermentation of sugars. Production of D(-)- and L(+)-lactic acid is based on the activity of LdhD and LdhL, respectively. In this work, LdhD enzyme activity was removed by using two different geneti ...
Shigella boydii Involved in O-antigen Synthesis By
... Figure 1. A model of inner and outer cell membranes of E.coli K-12. ......................... - 2 Figure 2. An example of a cell surface LPS, from E.coli O111:B4. ............................. - 3 Figure 3. Three O-antigen repeating unit structures that are identical in different serotypes of Shigel ...
... Figure 1. A model of inner and outer cell membranes of E.coli K-12. ......................... - 2 Figure 2. An example of a cell surface LPS, from E.coli O111:B4. ............................. - 3 Figure 3. Three O-antigen repeating unit structures that are identical in different serotypes of Shigel ...
ETO-Thesis-Final 061214. - JScholarship
... As stated above, dapivirine is another antiretroviral drug that has made significant progress in HIV PrEP trials. Dapivirine is one of the diarylpyrimidine non-nucleoside reverse transcriptase inhibitors (NNRTI), which inhibit HIV reverse transcriptase through allosteric binding. First generation N ...
... As stated above, dapivirine is another antiretroviral drug that has made significant progress in HIV PrEP trials. Dapivirine is one of the diarylpyrimidine non-nucleoside reverse transcriptase inhibitors (NNRTI), which inhibit HIV reverse transcriptase through allosteric binding. First generation N ...
Exogenous glutamine increases lipid accumulation in
... to 0.8% to ensure that the seed surface was in good contact with the medium. Sucrose concentration was set as 85 mM to support growth and to establish an optimum rate under which cultured seeds grow well in vitro. Usually, the well-developed capsule is trilocular containing one large mottled seed pe ...
... to 0.8% to ensure that the seed surface was in good contact with the medium. Sucrose concentration was set as 85 mM to support growth and to establish an optimum rate under which cultured seeds grow well in vitro. Usually, the well-developed capsule is trilocular containing one large mottled seed pe ...
... One cM equals 1% recombination and corresponds to approximately 0.8 × 106 base pairs (bp). The genetic map of the human genome spans roughly 3700 cM. Genetic maps are constructed by using linkage analysis: the analysis of the segregation of polymorphic markers in pedigrees. The first genetic markers ...
The DNA Binding Properties of Saccharomyces cerevisiae Rad51
... (dsDNA) and single-stranded DNA (4, 6) (for review, see Ref. 7). In vitro, the most extensively investigated DNA pairing reaction is the three-strand exchange reaction, in which the pairing of circular ssDNA and homologous dsDNA yields nicked circular dsDNA and linear ssDNA. During the formation of ...
... (dsDNA) and single-stranded DNA (4, 6) (for review, see Ref. 7). In vitro, the most extensively investigated DNA pairing reaction is the three-strand exchange reaction, in which the pairing of circular ssDNA and homologous dsDNA yields nicked circular dsDNA and linear ssDNA. During the formation of ...
regulation of fatty acid synthesis
... In addition to the β-carboxyltransferase subunit characterized by Sasaki, clones are now available for the biotin carboxylase (94), biotin carboxyl carrier protein (BCCP) (17), and α-subunit of the carboxyltransferase (95). The four subunits are assembled into a complex by gel filtration with a size ...
... In addition to the β-carboxyltransferase subunit characterized by Sasaki, clones are now available for the biotin carboxylase (94), biotin carboxyl carrier protein (BCCP) (17), and α-subunit of the carboxyltransferase (95). The four subunits are assembled into a complex by gel filtration with a size ...
Expanded roles of leucine-responsive regulatory protein in
... et al., 2002). A certain proportion of the regulated genes are involved, as originally proposed, in transport and metabolism of amino acids, but Lrp has also been suggested to regulate genes involved in biosynthesis and degradation of various metabolites other than amino acids (Brinkman et al., 2003 ...
... et al., 2002). A certain proportion of the regulated genes are involved, as originally proposed, in transport and metabolism of amino acids, but Lrp has also been suggested to regulate genes involved in biosynthesis and degradation of various metabolites other than amino acids (Brinkman et al., 2003 ...
The sucrose-cleaving enzymes of plants are crucial for development
... Most plant species contain at least two isoforms of sucrose synthase, which usually have highly homologous amino acid sequences and similar biochemical properties. By contrast, the regulation of their genes is markedly different and distinct developmental- and organ-specific expression patterns have ...
... Most plant species contain at least two isoforms of sucrose synthase, which usually have highly homologous amino acid sequences and similar biochemical properties. By contrast, the regulation of their genes is markedly different and distinct developmental- and organ-specific expression patterns have ...
Safety assessment - Federal Register of Legislation
... Monsanto Australia Limited is seeking approval in the Australia New Zealand Food Standards Code (the Code) for food derived from corn line 88017 (MON 88017), a variety that has been genetically modified (GM) for insect-protection and herbicide-tolerance. Standard 1.5.2 – Food Produced Using Gene Tec ...
... Monsanto Australia Limited is seeking approval in the Australia New Zealand Food Standards Code (the Code) for food derived from corn line 88017 (MON 88017), a variety that has been genetically modified (GM) for insect-protection and herbicide-tolerance. Standard 1.5.2 – Food Produced Using Gene Tec ...
Lecture 1 - "Hudel" Luecke
... Proteins are very complicated moleucules. With 20 different amino acids that can be arranged in any order to make a polypeptide of up to thousands of amino acids long, their potential for variety is extraordinary. This variety allows proteins to function as exquisitely specific enzymes that compose ...
... Proteins are very complicated moleucules. With 20 different amino acids that can be arranged in any order to make a polypeptide of up to thousands of amino acids long, their potential for variety is extraordinary. This variety allows proteins to function as exquisitely specific enzymes that compose ...
Participation of DDDD and KPAR
... Mercuric reductase (MerA) is an essential enzyme for the survival of microorganisms that reside in environments containing mercuric compounds. The enzyme converts the extremely toxic mercuric ions (Hg2+) into the less toxic volatile elemental mercury form (Hg 0). A novel MerA molecule that has under ...
... Mercuric reductase (MerA) is an essential enzyme for the survival of microorganisms that reside in environments containing mercuric compounds. The enzyme converts the extremely toxic mercuric ions (Hg2+) into the less toxic volatile elemental mercury form (Hg 0). A novel MerA molecule that has under ...
Nitrate Reductases: Structure, Functions, and Effect of Stress Factors
... been supposed recently that only high-potential clusters are able, for thermodynamic reasons, to transfer electrons, whereas the role of the other two clusters is still unknown. Subunit α (104-150 kD) coded by the narG gene contains the [4Fe-4S] cluster and molybdenum cofactor, just on which the nit ...
... been supposed recently that only high-potential clusters are able, for thermodynamic reasons, to transfer electrons, whereas the role of the other two clusters is still unknown. Subunit α (104-150 kD) coded by the narG gene contains the [4Fe-4S] cluster and molybdenum cofactor, just on which the nit ...
Multiomics of tomato glandular trichomes reveals
... chain (C2-C12) acyl sugars, and sesquiterpene carboxylic acids are prevalent in LC-MS-based chromatograms of LA1777 (Supplemental Figure 2). These results confirm previous metabolite analyses of tomato trichomes and underline that trichomes of these species produce different terpenoid secondary m ...
... chain (C2-C12) acyl sugars, and sesquiterpene carboxylic acids are prevalent in LC-MS-based chromatograms of LA1777 (Supplemental Figure 2). These results confirm previous metabolite analyses of tomato trichomes and underline that trichomes of these species produce different terpenoid secondary m ...
regulation of fatty acid synthesis
... ACCase reaction. Eastwell & Stumpf (26) found that chloroplast and wheat germ ACCase were inhibited by ADP and suggested this may account for light-dark regulation of the enzyme. Nikolau & Hawke (63) characterized the pH, Mg, ATP, and ADP dependence of maize ACCase activity and concluded that change ...
... ACCase reaction. Eastwell & Stumpf (26) found that chloroplast and wheat germ ACCase were inhibited by ADP and suggested this may account for light-dark regulation of the enzyme. Nikolau & Hawke (63) characterized the pH, Mg, ATP, and ADP dependence of maize ACCase activity and concluded that change ...
as a PDF
... (Yang, 1986; Yu and Yang, 1993) . The preparations of kinase FA phosphorylated myelin basic protein with a specific activity of - 1,600 nmol/min/mg and with a specific activity of 26,000 nmol/min/mg toward activation of inactive type1 protein phosphatase . When analyzed by SDS-PAGE and Coomassie Blu ...
... (Yang, 1986; Yu and Yang, 1993) . The preparations of kinase FA phosphorylated myelin basic protein with a specific activity of - 1,600 nmol/min/mg and with a specific activity of 26,000 nmol/min/mg toward activation of inactive type1 protein phosphatase . When analyzed by SDS-PAGE and Coomassie Blu ...
Modulation of functional properties of bifunctional S- Adenosylmethionine decarboxylase/Ornithine decarboxylase of
... Thrombospondin-related adhesion protein ...
... Thrombospondin-related adhesion protein ...
A 1-Megadalton Translocation Complex Containing
... preproteins with the translocon components, the so-called early import intermediate, was generated under low ATP concentrations (Waegemann and Soll, 1991; Perry and Keegstra, 1994; Schnell et al., 1994; Ma et al., 1996; Akita et al., 1997; Kouranov and Schnell, 1997; Nielsen et al., 1997; Young et a ...
... preproteins with the translocon components, the so-called early import intermediate, was generated under low ATP concentrations (Waegemann and Soll, 1991; Perry and Keegstra, 1994; Schnell et al., 1994; Ma et al., 1996; Akita et al., 1997; Kouranov and Schnell, 1997; Nielsen et al., 1997; Young et a ...
nitrogen fixation and its improvement through genetic engineering
... Nitrogen fixation is an “old” topic in scientific terms since it is over a century ago that scientists experimentally proved that some “unique” species of plants with the help of microbes that were later found bearing nitrogenase, are capable of utilizing atmospheric nitrogen. The thought of enginee ...
... Nitrogen fixation is an “old” topic in scientific terms since it is over a century ago that scientists experimentally proved that some “unique” species of plants with the help of microbes that were later found bearing nitrogenase, are capable of utilizing atmospheric nitrogen. The thought of enginee ...
Mechanisms and applications of disulfide bond formation
... protein (Pace et al. 1996). In the cell, the process of folding often initiates cotranslationally, so that the N-terminus of the protein begins to fold while the Cterminus of the protein is still being synthesized by the ribosome (Alberts et al. 2002). Across different species proteins fold in multi ...
... protein (Pace et al. 1996). In the cell, the process of folding often initiates cotranslationally, so that the N-terminus of the protein begins to fold while the Cterminus of the protein is still being synthesized by the ribosome (Alberts et al. 2002). Across different species proteins fold in multi ...
Transcriptomic and Reverse Genetic Analyses of Branched
... have taken a comparative genomic approach to identify critical enzymatic steps followed by gene silencing and metabolite analysis in S. pennellii and Nicotiana benthamiana. Our study verified the existence of distinct mechanisms of acyl sugar synthesis in Solanaceae. From microarray analyses, genes ...
... have taken a comparative genomic approach to identify critical enzymatic steps followed by gene silencing and metabolite analysis in S. pennellii and Nicotiana benthamiana. Our study verified the existence of distinct mechanisms of acyl sugar synthesis in Solanaceae. From microarray analyses, genes ...