Raw Glycerol as Substrate for the Production of Yeast Biomass
... used for protein supplementation of a staple diet by replacing costly conventional sources like soymeal and fishmeal to alleviate the problem of protein scarcity. Moreover, the bioconversion of agricultural and industrial wastes to protein-rich food has an additional benefit of making the final prod ...
... used for protein supplementation of a staple diet by replacing costly conventional sources like soymeal and fishmeal to alleviate the problem of protein scarcity. Moreover, the bioconversion of agricultural and industrial wastes to protein-rich food has an additional benefit of making the final prod ...
The Role in Translation of Editing and Multi
... to favorable binding affinities of cognate over non-cognate substrates in the active site of the enzyme. Its cognate amino acid Tyr differs from Phe by a single hydroxyl group, and the specific recognition and binding of the hydroxyl group allows bacterial TyrRS to discriminate against non-cognate P ...
... to favorable binding affinities of cognate over non-cognate substrates in the active site of the enzyme. Its cognate amino acid Tyr differs from Phe by a single hydroxyl group, and the specific recognition and binding of the hydroxyl group allows bacterial TyrRS to discriminate against non-cognate P ...
Nutrient Sensing through the Plasma Membrane of Eukaryotic Cells
... are marked for degradation in the proteasome (V. Brachet, unpublished work) by their ubiquitination, catalysed by the SCFGrr1 ubiquitin-protein ligase [19]. Proteins must be phosphorylated to be substrates of this enzyme [28], and for Mth1 and Std1 this is achieved by YckI, encoded by YCK1 and YCK2. ...
... are marked for degradation in the proteasome (V. Brachet, unpublished work) by their ubiquitination, catalysed by the SCFGrr1 ubiquitin-protein ligase [19]. Proteins must be phosphorylated to be substrates of this enzyme [28], and for Mth1 and Std1 this is achieved by YckI, encoded by YCK1 and YCK2. ...
Ube2W conjugates ubiquitin to α-amino groups of protein N
... To identify sites of RNF4-dependent ubiquitin attachment to poly-SUMO-2 in reactions containing Ube2W and UbcH5a, MS was employed. Ubiquitylated SUMO-2 species were excised from gel slices shown in Figures 2(B) and 2(C), digested with trypsin and the resultant peptides analysed by MS (see the Experi ...
... To identify sites of RNF4-dependent ubiquitin attachment to poly-SUMO-2 in reactions containing Ube2W and UbcH5a, MS was employed. Ubiquitylated SUMO-2 species were excised from gel slices shown in Figures 2(B) and 2(C), digested with trypsin and the resultant peptides analysed by MS (see the Experi ...
Journal of Biological Chemistry
... inhibition of incorporation, whereas adenosine does so only partially. The results with adenosine have been somewhat variable, in that sometimes it is found to be completely effective whereas at other times only partially so. This may be due to the very low solubility of adenosine in water. Other de ...
... inhibition of incorporation, whereas adenosine does so only partially. The results with adenosine have been somewhat variable, in that sometimes it is found to be completely effective whereas at other times only partially so. This may be due to the very low solubility of adenosine in water. Other de ...
Journal of Biotechnology
... prosthetic group to which the adenylated amino acid substrate is transferred and bound as thioester. A 4 -phosphopantetheinetransferase (PPTase) converts the apo-form of the PCP into its holo-form by loading the Ppan-cofactor to an active serine. Sfp is a prototype of PPTases with wide substrate to ...
... prosthetic group to which the adenylated amino acid substrate is transferred and bound as thioester. A 4 -phosphopantetheinetransferase (PPTase) converts the apo-form of the PCP into its holo-form by loading the Ppan-cofactor to an active serine. Sfp is a prototype of PPTases with wide substrate to ...
Gene Section MUC4 (mucin 4, cell surface associated) in Oncology and Haematology
... interactions and signaling activation lead to malignant transformation. Hence, dysregulation of MUC4 expression, a protective cell surface protein, may have deleterious effects. The association of MUC4 with ERBB2 involves its translocation from the basolateral toward the apical membrane and increasi ...
... interactions and signaling activation lead to malignant transformation. Hence, dysregulation of MUC4 expression, a protective cell surface protein, may have deleterious effects. The association of MUC4 with ERBB2 involves its translocation from the basolateral toward the apical membrane and increasi ...
Review Non-ribosomal peptide synthetases: Identifying the cryptic
... (BGCs) of a known biosynthetic class, in a newly sequenced genome. These computational tools are based on the presence of specific signature domains which form the basis for the development of gene identification algorithms. Further on, the biggest challenge remains the prediction of the encoded nat ...
... (BGCs) of a known biosynthetic class, in a newly sequenced genome. These computational tools are based on the presence of specific signature domains which form the basis for the development of gene identification algorithms. Further on, the biggest challenge remains the prediction of the encoded nat ...
Genomic Insights into Methanotrophy: The Complete Genome Sequence of Methylococcus capsulatus (Bath)
... Methanotrophs are ubiquitous bacteria that can use the greenhouse gas methane as a sole carbon and energy source for growth, thus playing major roles in global carbon cycles, and in particular, substantially reducing emissions of biologically generated methane to the atmosphere. Despite their import ...
... Methanotrophs are ubiquitous bacteria that can use the greenhouse gas methane as a sole carbon and energy source for growth, thus playing major roles in global carbon cycles, and in particular, substantially reducing emissions of biologically generated methane to the atmosphere. Despite their import ...
THE INFLUENCE OF NUTRITIONAL PHOSPHATE DEPRIVATION ARABIDOPSIS THALIANA
... cultures by 2-dimensional gel electrophoresis. Mass spectrometry identified 18 different secreted proteins that were upregulated by at least 2-fold by –Pi Arabidopsis. They were predicted to function in Pi scavenging, cell wall and ROS metabolism, proteolysis, and pathogen responses. The relationshi ...
... cultures by 2-dimensional gel electrophoresis. Mass spectrometry identified 18 different secreted proteins that were upregulated by at least 2-fold by –Pi Arabidopsis. They were predicted to function in Pi scavenging, cell wall and ROS metabolism, proteolysis, and pathogen responses. The relationshi ...
A conserved N-‐terminal domain of Rif2 regulates telomere length in
... Telomere sequence DNA repeats are bound by a set of proteins that mediate two essential functions; first to protect the ends from degradation, recombination, and initiation of a damage response, and second ...
... Telomere sequence DNA repeats are bound by a set of proteins that mediate two essential functions; first to protect the ends from degradation, recombination, and initiation of a damage response, and second ...
Herculex® I Product Overview
... protein is proteolytically cleaved to its active form like many other Cry proteins studied (Gill et al., 1992; English and Slatin, 1992; Knowles, 1994; Dean et al., 1996). Studies of several activated Cry protein toxins similar to Cry1F have demonstrated that they interact specifically with insect m ...
... protein is proteolytically cleaved to its active form like many other Cry proteins studied (Gill et al., 1992; English and Slatin, 1992; Knowles, 1994; Dean et al., 1996). Studies of several activated Cry protein toxins similar to Cry1F have demonstrated that they interact specifically with insect m ...
a curated database for protein phosphorylation sites in prokaryotes
... manually collected dataset and integrated into dbPSP database. In total, 7391 non-redundant phosphorylation sites among seven types of residues were found in 3750 substrates from 11 phyla were provided in the database, which present a comprehensive data resource for prokaryotic phosphorylation. In t ...
... manually collected dataset and integrated into dbPSP database. In total, 7391 non-redundant phosphorylation sites among seven types of residues were found in 3750 substrates from 11 phyla were provided in the database, which present a comprehensive data resource for prokaryotic phosphorylation. In t ...
Molecular Characterisation of the 76 kDa Iron
... Fig. 3 Amino acid sequence alignment of the N-terminal regions of homologues to the potato 76 kDa subunit. For the polypeptides encoded by nuclear genes (St, Bt and Nc) the amino acid sequences of the mature proteins are shown. The conserved cysteine and histidine residues are marked by asterisks ab ...
... Fig. 3 Amino acid sequence alignment of the N-terminal regions of homologues to the potato 76 kDa subunit. For the polypeptides encoded by nuclear genes (St, Bt and Nc) the amino acid sequences of the mature proteins are shown. The conserved cysteine and histidine residues are marked by asterisks ab ...
International Journal of Antimicrobial Agents Mechanism of drug
... pathogen is limited because the enteritis caused by this organism is not as frequent as that caused by Vibrio cholerae [5]. However, in recent years it is being isolated with greater frequency from patients with cholera-like illness, many of which display multiple drug resistance [5–8]. There are se ...
... pathogen is limited because the enteritis caused by this organism is not as frequent as that caused by Vibrio cholerae [5]. However, in recent years it is being isolated with greater frequency from patients with cholera-like illness, many of which display multiple drug resistance [5–8]. There are se ...
Phosphate Groups Modifying Myelin Basic Proteins Are
... in sequence, the myelin basic proteins (12, 31). These proteins are located at the apposition of the very closely apposed cytoplasmic faces of the myelin membrane (48). The peptide backbones of the basic proteins have been shown to be metabolically very stable, with half-lives of many months (22, 53 ...
... in sequence, the myelin basic proteins (12, 31). These proteins are located at the apposition of the very closely apposed cytoplasmic faces of the myelin membrane (48). The peptide backbones of the basic proteins have been shown to be metabolically very stable, with half-lives of many months (22, 53 ...
Introduction to Protein Structure
... the tertiary structure of a protein resides in the chemistry of its amino acid sequence. […] It was demonstrated that, after cleavage of disulfide bonds and disruption of tertiary structure, many proteins could spontaneously refold to their native forms. This work resulted in general acceptance of t ...
... the tertiary structure of a protein resides in the chemistry of its amino acid sequence. […] It was demonstrated that, after cleavage of disulfide bonds and disruption of tertiary structure, many proteins could spontaneously refold to their native forms. This work resulted in general acceptance of t ...
Cress and Potato Soluble Epoxide Hydrolases
... S100 (Pharmacia, Uppsala, Sweden) column (1.5 ⫻ 100 cm), equilibrated with Buffer A (flow rate, 10 ml/h; fraction volume, 1 ml). The molecular weight was calculated by comparing the elution of the EH activity with that of the following standard proteins: alcohol dehydrogenase (150 kDa), BSA (66 kDa) ...
... S100 (Pharmacia, Uppsala, Sweden) column (1.5 ⫻ 100 cm), equilibrated with Buffer A (flow rate, 10 ml/h; fraction volume, 1 ml). The molecular weight was calculated by comparing the elution of the EH activity with that of the following standard proteins: alcohol dehydrogenase (150 kDa), BSA (66 kDa) ...
University of Groningen Stress responses and sugar metabolism in
... In addition to these phosphoproteins also low molecular weight molecules such as NADP, glucose‐6‐phosphate (G6P) and fructose‐1,6‐bisphosphate (FBP) can modulate either DNA‐ binding properties or the interaction with the transcriptional machinery of the CcpA‐(HPr‐ Ser ...
... In addition to these phosphoproteins also low molecular weight molecules such as NADP, glucose‐6‐phosphate (G6P) and fructose‐1,6‐bisphosphate (FBP) can modulate either DNA‐ binding properties or the interaction with the transcriptional machinery of the CcpA‐(HPr‐ Ser ...
MS#5_(Cueno and Laude).indd - Philippine Journal of Science
... (Banzon and Velasco 1982; Harries 1994) further suggesting ontogenetic gene expression which coincides with the hypothesis made by Villalobos et al. (2001). This would suggest a temporal pattern of gene regulation among coconut genes involved in fatty acid synthesis. The untranslated region (UTR) is ...
... (Banzon and Velasco 1982; Harries 1994) further suggesting ontogenetic gene expression which coincides with the hypothesis made by Villalobos et al. (2001). This would suggest a temporal pattern of gene regulation among coconut genes involved in fatty acid synthesis. The untranslated region (UTR) is ...
Nonribosomal peptide synthesis in Aspergillus
... been identified in fungi (14 in Aspergillus fumigatus). LeaA, a methyltransferase, is involved in secondary metabolite gene cluster regulation in Aspergillus spp. The NRP synthetases GliP and FtmA respectively direct the biosynthesis of the toxic metabolites gliotoxin and brevianamide F, a precursor ...
... been identified in fungi (14 in Aspergillus fumigatus). LeaA, a methyltransferase, is involved in secondary metabolite gene cluster regulation in Aspergillus spp. The NRP synthetases GliP and FtmA respectively direct the biosynthesis of the toxic metabolites gliotoxin and brevianamide F, a precursor ...
The role of AMP-activated protein kinase in the coordination of
... mTOR promotes its dissociation from eIF4E bound to the mRNA 7-methylguanosine cap structure, allowing the assembly of the preinitiation complex (128). S6K1 activation needs initial phosphorylation by mTORC1 at Thr389 (101) and additional inputs on Thr229 for full activation by the phosphoinositide-d ...
... mTOR promotes its dissociation from eIF4E bound to the mRNA 7-methylguanosine cap structure, allowing the assembly of the preinitiation complex (128). S6K1 activation needs initial phosphorylation by mTORC1 at Thr389 (101) and additional inputs on Thr229 for full activation by the phosphoinositide-d ...
... iii) What assumption did you make about the type of binding when you sketched these curves? (2 pts). The hyperbolic curves in the binding curve, and the slope of 1 in the Hill plot indicate noncooperative binding iv) Which kinetic parameter, the on-rate or the off-rate, would you expect to change as ...
Trans-chalcone and quercetin down-regulate fatty acid synthase
... fungi of the family Arthrodermataceae (Dermatophytes), which are able to digest keratin. This mycosis is a common infection worldwide [1]. The main etiological agent of dermatophytosis is the anthropophilic and cosmopolitan fungus Trichophyton rubrum, which accounts for 69.5% of all dermatophytic in ...
... fungi of the family Arthrodermataceae (Dermatophytes), which are able to digest keratin. This mycosis is a common infection worldwide [1]. The main etiological agent of dermatophytosis is the anthropophilic and cosmopolitan fungus Trichophyton rubrum, which accounts for 69.5% of all dermatophytic in ...
GIBBERELLIN BIOSYNTHESIS: Enzymes, Genes and Their
... protection assays (A Silverstone & TP Sun, personal communication) or RTPCR, respectively, KS mRNA, though of low abundance, can be assayed in pumpkin leaves by northern hybridization (147). This is consistent with strict regulation of the first step of ent-kaurene synthesis from the abundant GGDP. ...
... protection assays (A Silverstone & TP Sun, personal communication) or RTPCR, respectively, KS mRNA, though of low abundance, can be assayed in pumpkin leaves by northern hybridization (147). This is consistent with strict regulation of the first step of ent-kaurene synthesis from the abundant GGDP. ...