Transcriptomic and Reverse Genetic Analyses of Branched
... have taken a comparative genomic approach to identify critical enzymatic steps followed by gene silencing and metabolite analysis in S. pennellii and Nicotiana benthamiana. Our study verified the existence of distinct mechanisms of acyl sugar synthesis in Solanaceae. From microarray analyses, genes ...
... have taken a comparative genomic approach to identify critical enzymatic steps followed by gene silencing and metabolite analysis in S. pennellii and Nicotiana benthamiana. Our study verified the existence of distinct mechanisms of acyl sugar synthesis in Solanaceae. From microarray analyses, genes ...
An investigation of the zinc binding characteristics of the RING finger
... RBBP6 contains a RING finger domain, which is a hallmark of E3 ubiquitin ligases, and which is essential for the interaction with Hdm2 (Li et al., 2007). RING fingers typically bind two Zn2+ ions, coordinated by eight conserved cysteine or histidine residues, typically forming a C3HC4 motif, althoug ...
... RBBP6 contains a RING finger domain, which is a hallmark of E3 ubiquitin ligases, and which is essential for the interaction with Hdm2 (Li et al., 2007). RING fingers typically bind two Zn2+ ions, coordinated by eight conserved cysteine or histidine residues, typically forming a C3HC4 motif, althoug ...
The Family of Berberine Bridge Enzyme-like
... AtBBE-like protein 15 was identified as a monolignol oxidoreductase. Subsequently AtBBE-like protein 13 was characterized by my dear colleague Barbara Steiner and was shown to possess the same function. AtBBE-like protein 15 is localized in the plant cell wall and catalyses the oxidation of one of t ...
... AtBBE-like protein 15 was identified as a monolignol oxidoreductase. Subsequently AtBBE-like protein 13 was characterized by my dear colleague Barbara Steiner and was shown to possess the same function. AtBBE-like protein 15 is localized in the plant cell wall and catalyses the oxidation of one of t ...
Integration of omics data with biochemical reaction
... Printing: Háskólaprent, Fálkagata 2, 107 Reykjavik Reykjavik, Iceland, June 2014 ...
... Printing: Háskólaprent, Fálkagata 2, 107 Reykjavik Reykjavik, Iceland, June 2014 ...
Hypoxia in skeletal muscles: from physiology to gene expression
... muscles in chronic obstructive pulmonary disease (COPD) patients who suffer from chronic hypoxia [36]. Consistently, Magalhaes et al. report a functional impairment of mitochondrial complex V in skeletal muscles during an acute hypoxia induced by eccentric exercise [12]. A switch of mitochondrial co ...
... muscles in chronic obstructive pulmonary disease (COPD) patients who suffer from chronic hypoxia [36]. Consistently, Magalhaes et al. report a functional impairment of mitochondrial complex V in skeletal muscles during an acute hypoxia induced by eccentric exercise [12]. A switch of mitochondrial co ...
Responses of cows to abomasal infusion of lysine and methionine at
... by increasing dietary CP up to 140 g/kg dry matter ( D M ) relative to diets deficient in CP (90 g/kg D M ) ; however, further increases in dietary CP content often showed inconsistent responses (Clark and Davis, 1980). Increasing the supply o f CP to the small intestine by adding undegradable prote ...
... by increasing dietary CP up to 140 g/kg dry matter ( D M ) relative to diets deficient in CP (90 g/kg D M ) ; however, further increases in dietary CP content often showed inconsistent responses (Clark and Davis, 1980). Increasing the supply o f CP to the small intestine by adding undegradable prote ...
Virology Journal
... the cell membrane [21]. These two proteins work in conjunction to destroy the cell wall of bacteria and subsequently lyse the cell [24]. These components of a host lysis cassette are each present in the genome of phages eiAU, eiDWF, and eiMSLS including a putative Rz lysis accessory protein encoded ...
... the cell membrane [21]. These two proteins work in conjunction to destroy the cell wall of bacteria and subsequently lyse the cell [24]. These components of a host lysis cassette are each present in the genome of phages eiAU, eiDWF, and eiMSLS including a putative Rz lysis accessory protein encoded ...
Desert hedgehog is a mammal-specific gene expressed during testicular and ovarian
... Background: Desert hedgehog (DHH) belongs to the hedgehog gene family that act as secreted intercellular signal transducers. DHH is an essential morphogen for normal testicular development and function in both mice and humans but is not present in the avian lineage. Like other hedgehog proteins, DHH ...
... Background: Desert hedgehog (DHH) belongs to the hedgehog gene family that act as secreted intercellular signal transducers. DHH is an essential morphogen for normal testicular development and function in both mice and humans but is not present in the avian lineage. Like other hedgehog proteins, DHH ...
Regulation of Starch Synthesis in Cassava Yona Baguma
... composed of intermediate size α-1,4 linked glucans that are clustered together and hooked to longer spacer glucans by α-1,6 linkages, and is responsible for the granular nature of starch (Ball & Morell, 2003). Amylose (105 - 106 Da), which constitutes between 11% and 37% of storage starch (Shannon & ...
... composed of intermediate size α-1,4 linked glucans that are clustered together and hooked to longer spacer glucans by α-1,6 linkages, and is responsible for the granular nature of starch (Ball & Morell, 2003). Amylose (105 - 106 Da), which constitutes between 11% and 37% of storage starch (Shannon & ...
Desert hedgehogis a mammal-specific gene expressed during
... Background: Desert hedgehog (DHH) belongs to the hedgehog gene family that act as secreted intercellular signal transducers. DHH is an essential morphogen for normal testicular development and function in both mice and humans but is not present in the avian lineage. Like other hedgehog proteins, DHH ...
... Background: Desert hedgehog (DHH) belongs to the hedgehog gene family that act as secreted intercellular signal transducers. DHH is an essential morphogen for normal testicular development and function in both mice and humans but is not present in the avian lineage. Like other hedgehog proteins, DHH ...
Tansley Review No. 112 Oxygen processing in photosynthesis
... metabolism to use light energy is constrained by the environment, which has necessitated the evolution of an integrated process of great flexibility, able to operate over a broad window of environmental conditions. At low light levels, in unstressed conditions, photosynthesis operates with maximal q ...
... metabolism to use light energy is constrained by the environment, which has necessitated the evolution of an integrated process of great flexibility, able to operate over a broad window of environmental conditions. At low light levels, in unstressed conditions, photosynthesis operates with maximal q ...
Engineering of polyketide biosynthetic pathways for bioactive
... shown a variety of biological activities. These molecules are synthesized by polyketide synthases (PKSs). PKSs are classified into three types based on their sequence, primary structure, and catalytic mechanism. Because of the bioactivities of polyketide natural products, this study is focused on th ...
... shown a variety of biological activities. These molecules are synthesized by polyketide synthases (PKSs). PKSs are classified into three types based on their sequence, primary structure, and catalytic mechanism. Because of the bioactivities of polyketide natural products, this study is focused on th ...
A Simple Introduction to NCBI BLAST
... any known genes. The unknown sequence is an 11,000 base pair (bp) fragment of genomic DNA, and the objective of gene annotation is to find and precisely map the coding regions of any genes in this part of the genome. When we design a BLAST search, there are three basic decisions we must make: the BL ...
... any known genes. The unknown sequence is an 11,000 base pair (bp) fragment of genomic DNA, and the objective of gene annotation is to find and precisely map the coding regions of any genes in this part of the genome. When we design a BLAST search, there are three basic decisions we must make: the BL ...
dbPSP: a curated database for protein phosphorylation sites in
... manually collected dataset and integrated into dbPSP database. In total, 7391 non-redundant phosphorylation sites among seven types of residues were found in 3750 substrates from 11 phyla were provided in the database, which present a comprehensive data resource for prokaryotic phosphorylation. In t ...
... manually collected dataset and integrated into dbPSP database. In total, 7391 non-redundant phosphorylation sites among seven types of residues were found in 3750 substrates from 11 phyla were provided in the database, which present a comprehensive data resource for prokaryotic phosphorylation. In t ...
Genomic structure, chromosomal localization, and conserved alternative splice forms of thrombopoietin
... promoter and thc SV40 polyadenylation signal. The resulting expressionvectors(PRKtkneo-mTPO.PRKtkneo-mTPO-2.PRKtkneohTPO. and PRKtkneo-hTPO-2) were transiently transfected into 293 cells (a human embryonic kidney cell line) using the calcium phosphate method. After transient transfection. medium was ...
... promoter and thc SV40 polyadenylation signal. The resulting expressionvectors(PRKtkneo-mTPO.PRKtkneo-mTPO-2.PRKtkneohTPO. and PRKtkneo-hTPO-2) were transiently transfected into 293 cells (a human embryonic kidney cell line) using the calcium phosphate method. After transient transfection. medium was ...
Queuosine formation in eukaryotic tRNA occurs via a mitochondria
... 271 amino acids, respectively. Aligning the QTRTD1 splice variants with the TGT proteins shows a low overall degree of conservation (Fig. 1). For example, mouse TGT and Qv1 share 23% sequence identity and 41% homology. The potential secondary structure of the QTRTD1 variants was examined using the c ...
... 271 amino acids, respectively. Aligning the QTRTD1 splice variants with the TGT proteins shows a low overall degree of conservation (Fig. 1). For example, mouse TGT and Qv1 share 23% sequence identity and 41% homology. The potential secondary structure of the QTRTD1 variants was examined using the c ...
Prokaryotic proteins of antioxidant defense in Trichomonas vaginalis
... TvOsmC (a member of OsmC/Ohr protein family) represent proteins rarely encountered in eukaryotes, that were probably acquired by T. vaginalis predecessor trough lateral gene transfer from a prokaryotic donor. TvFDP, the terminal hydrogenosomal oxidase, catalyzes reduction of oxygen to water. TvIsf3, ...
... TvOsmC (a member of OsmC/Ohr protein family) represent proteins rarely encountered in eukaryotes, that were probably acquired by T. vaginalis predecessor trough lateral gene transfer from a prokaryotic donor. TvFDP, the terminal hydrogenosomal oxidase, catalyzes reduction of oxygen to water. TvIsf3, ...
Crystal structure of mouse coronavirus receptor
... The Coronaviridae family of large, enveloped, positive-stranded RNA viruses consists of at least three major genera or groups (Table S1). Aminopeptidase-N (APN) is the receptor for porcine transmissible gastroenteritis virus (TGEV), porcine respiratory coronavirus (PRCoV), and human coronavirus 229E ...
... The Coronaviridae family of large, enveloped, positive-stranded RNA viruses consists of at least three major genera or groups (Table S1). Aminopeptidase-N (APN) is the receptor for porcine transmissible gastroenteritis virus (TGEV), porcine respiratory coronavirus (PRCoV), and human coronavirus 229E ...
The Ethylene Signal Transduction Pathway in Plants
... ACC oxidase is constitutively present in most tissues, but its synthesis is increased during fruit ripening in tomato. The genes that encode A C C synthase and A C C oxidase have been cloned and characterized from many plant species (5, 6). A C C synthase is encoded by multigene families in all spec ...
... ACC oxidase is constitutively present in most tissues, but its synthesis is increased during fruit ripening in tomato. The genes that encode A C C synthase and A C C oxidase have been cloned and characterized from many plant species (5, 6). A C C synthase is encoded by multigene families in all spec ...
PhD Thesis - Cox Group
... Polyketides are formed by a similar biosynthetic pathway to that of fatty acids. They are formed by repetitive decarboxylative condensation of simple acetate units in the form of acetyl CoA 24 and malonyl CoA 25 (in some cases propionate and butyrate units) in a head to tail manner. In polyketide p ...
... Polyketides are formed by a similar biosynthetic pathway to that of fatty acids. They are formed by repetitive decarboxylative condensation of simple acetate units in the form of acetyl CoA 24 and malonyl CoA 25 (in some cases propionate and butyrate units) in a head to tail manner. In polyketide p ...
The Amino Acid Sequences of the Myelin
... nervous system adhesion molecules, including MAG, share general structural and amino acid sequence homologies has not yet been elucidated. Two MAG polypeptides (Mr 72,000 and 67,000) are detectable in in vitro translation systems programmed with total brain mRNA (10). Presumably, these polypeptides, ...
... nervous system adhesion molecules, including MAG, share general structural and amino acid sequence homologies has not yet been elucidated. Two MAG polypeptides (Mr 72,000 and 67,000) are detectable in in vitro translation systems programmed with total brain mRNA (10). Presumably, these polypeptides, ...
Two nearly identical terpene synthases catalyze the
... AmNES/LIS proteins For functional characterization of AmNES/LIS proteins, the coding region of each gene was subcloned into an expression vector, and encoded proteins were expressed in Escherichia coli and analyzed for TPS activities. Although the crude bacterial extract containing AmNES/LIS-1 prote ...
... AmNES/LIS proteins For functional characterization of AmNES/LIS proteins, the coding region of each gene was subcloned into an expression vector, and encoded proteins were expressed in Escherichia coli and analyzed for TPS activities. Although the crude bacterial extract containing AmNES/LIS-1 prote ...
A tribute to sulfur - Wiley Online Library
... was found that the active site Cys residue is located in the middle of a highly flexible loop of 12 amino acids, which may be able to adapt to varied geometries in acceptor proteins. The 3D structure of C-DES from Synechocystis has now also become available [52]. Contrary to the NifS type Cys desulf ...
... was found that the active site Cys residue is located in the middle of a highly flexible loop of 12 amino acids, which may be able to adapt to varied geometries in acceptor proteins. The 3D structure of C-DES from Synechocystis has now also become available [52]. Contrary to the NifS type Cys desulf ...
AMINO ACIDS, PEPTIDES, AND PROTEINS
... The Amino Acid Residues in Proteins Are L Stereoisomers Nearly all biological compounds with a chiral center occur naturally in only one stereoisomeric form, either D or L. The amino acid residues in protein molecules are exclusively L stereoisomers. D-Amino acid residues have been found only in a f ...
... The Amino Acid Residues in Proteins Are L Stereoisomers Nearly all biological compounds with a chiral center occur naturally in only one stereoisomeric form, either D or L. The amino acid residues in protein molecules are exclusively L stereoisomers. D-Amino acid residues have been found only in a f ...