Research in Microbiology
... phosphorelay system, which is highly conserved in sporeforming Bacillus species (Stephenson and Hoch, 2002). In the system, histidine kinase phosphorylates Spo0F, and the phosphate group from the Spo0F is transferred to Spo0A by Spo0B. Spo0F and Spo0A are very highly conserved in the amino acid sequ ...
... phosphorelay system, which is highly conserved in sporeforming Bacillus species (Stephenson and Hoch, 2002). In the system, histidine kinase phosphorylates Spo0F, and the phosphate group from the Spo0F is transferred to Spo0A by Spo0B. Spo0F and Spo0A are very highly conserved in the amino acid sequ ...
Homology Modelling and Structural Comparisons of Capsid
... In 2011 [18] the crystal structure of PCV2 Cap was determined. To crystallize PCV2 Cap, 40 amino acids were omitted from the N-terminal, which contains the NLS, however, the structure placed the NLS inside the capsid [18]. The structural data provided important information with respect to the antige ...
... In 2011 [18] the crystal structure of PCV2 Cap was determined. To crystallize PCV2 Cap, 40 amino acids were omitted from the N-terminal, which contains the NLS, however, the structure placed the NLS inside the capsid [18]. The structural data provided important information with respect to the antige ...
Protein composition and function of red and white skeletal - AJP-Cell
... oxidative phosphorylation between red and white muscle. Indeed, Jackman and Willis (24) found that mitochondria isolated from rabbit soleus had greater electron transport chain (ETC) activity and maximal (State 3) respiration with pyruvate ⫹ malate (P⫹M) or palmitoyl carnitine ⫹ malate (PC⫹M) as oxi ...
... oxidative phosphorylation between red and white muscle. Indeed, Jackman and Willis (24) found that mitochondria isolated from rabbit soleus had greater electron transport chain (ETC) activity and maximal (State 3) respiration with pyruvate ⫹ malate (P⫹M) or palmitoyl carnitine ⫹ malate (PC⫹M) as oxi ...
Structure, mechanism and regulation of pyruvate carboxylase
... Initially, it was estimated that equal proportions of pyruvate enter the TCA cycle through PC and the PDH (pyruvate dehydrogenase) complex, and that these two fluxes are equally important for glucose-induced insulin secretion [32]. However, subsequent experiments using NMR analysis showed that pyruv ...
... Initially, it was estimated that equal proportions of pyruvate enter the TCA cycle through PC and the PDH (pyruvate dehydrogenase) complex, and that these two fluxes are equally important for glucose-induced insulin secretion [32]. However, subsequent experiments using NMR analysis showed that pyruv ...
IN VIVO ENOL CASTOR OIL SEEDS AT THREONINE-4 AND SERINE-451
... In addition PEPC has been shown to contribute to fatty acid synthesis through generation of metabolic precursors and reducing power. Fatty acid synthesis within the leucoplast is ATP and NAD(P)H dependent and uses acetyl-CoA as a precursor (Fig. 1.3B). The pathway from sucrose-derived cytosolic hexo ...
... In addition PEPC has been shown to contribute to fatty acid synthesis through generation of metabolic precursors and reducing power. Fatty acid synthesis within the leucoplast is ATP and NAD(P)H dependent and uses acetyl-CoA as a precursor (Fig. 1.3B). The pathway from sucrose-derived cytosolic hexo ...
Understanding the acetylome: translating targeted
... POSTTRANSLATIONAL MODIFICATION (PTM) of protein targets is a fundamental mechanism regulating cellular biological function. It is readily accepted that PTM is a pivotal process conveying environmental stresses to physiologically diverse processes such as cell cycle regulation, growth, autophagy, and ...
... POSTTRANSLATIONAL MODIFICATION (PTM) of protein targets is a fundamental mechanism regulating cellular biological function. It is readily accepted that PTM is a pivotal process conveying environmental stresses to physiologically diverse processes such as cell cycle regulation, growth, autophagy, and ...
RELIC – A bioinformatics server for combinatorial
... selected peptides; serially performs this function for multiple protein sequences using the same peptide sequence set, then compiles a list of those proteins so that they are ranked by similarity factor running from most similar to least similar. This program is capable of sorting an entire genome-d ...
... selected peptides; serially performs this function for multiple protein sequences using the same peptide sequence set, then compiles a list of those proteins so that they are ranked by similarity factor running from most similar to least similar. This program is capable of sorting an entire genome-d ...
Abstract
... be a viable approach to slow animal aging, and raises questions about folate supplementation. Aging in Caenorhabditis elegans - nature versus nuture (or a diet of E. coli)? The first genes extending lifespan were identified in the nematode Caenorhabditis elegans [1], and several of the metabolic pat ...
... be a viable approach to slow animal aging, and raises questions about folate supplementation. Aging in Caenorhabditis elegans - nature versus nuture (or a diet of E. coli)? The first genes extending lifespan were identified in the nematode Caenorhabditis elegans [1], and several of the metabolic pat ...
709_2010_211_MOESM2_ESM - Springer Static Content Server
... ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN 6: AHP6 lacks the conserved histidine residue (Asn83 in AHP6b), which is required for phosphotransfer, present in the other AHPs. AHP6 does not appear to have phosphotransfer activity. Acts as an inhibitor of cytokinin signalling by interacting with the ...
... ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN 6: AHP6 lacks the conserved histidine residue (Asn83 in AHP6b), which is required for phosphotransfer, present in the other AHPs. AHP6 does not appear to have phosphotransfer activity. Acts as an inhibitor of cytokinin signalling by interacting with the ...
Expanding the Genetic Code
... functional roles to amino acid residues in proteins. Selectivity depends on the number and reactivity (dependent on both steric and electronic factors) of a particular amino acid side chain. Typical modifications include the oxidation or alkylation of cysteine residues, acylation of either the N-ter ...
... functional roles to amino acid residues in proteins. Selectivity depends on the number and reactivity (dependent on both steric and electronic factors) of a particular amino acid side chain. Typical modifications include the oxidation or alkylation of cysteine residues, acylation of either the N-ter ...
1-3 flagellum - Instituto de Higiene
... Alternatively, the acyl modifications on some proteins such as FCaBP could cause partitioning into the unique lipid environment of the flagellar membrane. Furthermore, other proteins like ISO2 are not present in the flagellar membrane and could be either actively excluded from this compartment or re ...
... Alternatively, the acyl modifications on some proteins such as FCaBP could cause partitioning into the unique lipid environment of the flagellar membrane. Furthermore, other proteins like ISO2 are not present in the flagellar membrane and could be either actively excluded from this compartment or re ...
Recombinant Brassinosteroid Insensitive 1 Receptor-Like
... been shown by genetic and molecular analysis to be a critical component of brassinosteroid signal transduction. In this study we examined some of the biochemical properties of the BRI1 kinase domain (BRI1-KD) in vitro, which might be important predictors of in vivo function. Recombinant BRI1-KD auto ...
... been shown by genetic and molecular analysis to be a critical component of brassinosteroid signal transduction. In this study we examined some of the biochemical properties of the BRI1 kinase domain (BRI1-KD) in vitro, which might be important predictors of in vivo function. Recombinant BRI1-KD auto ...
Identification and expression of GH
... products (1.4 kb) were cloned into pACE after double digestion of both vector and insert with NdeI and PstI. The resulting plasmid library was transformed into E. coli JM109 and E. coli Top10-competent cells by electroporation. The transformants were plated on LB medium containing 0.3% (w/v) soluble ...
... products (1.4 kb) were cloned into pACE after double digestion of both vector and insert with NdeI and PstI. The resulting plasmid library was transformed into E. coli JM109 and E. coli Top10-competent cells by electroporation. The transformants were plated on LB medium containing 0.3% (w/v) soluble ...
University of Groningen Lactococcus lactis systems biology Eckhardt
... genomes. An important achievement in the field of genomics is the discovery of DNA as the carrier of genetic information and the decryption of its structure and code 40. Decoding, correctly assembling and annotating the entire genomic code of organisms is another important aspect of genomics. The fi ...
... genomes. An important achievement in the field of genomics is the discovery of DNA as the carrier of genetic information and the decryption of its structure and code 40. Decoding, correctly assembling and annotating the entire genomic code of organisms is another important aspect of genomics. The fi ...
The Plant Cell
... coli resistance to a group of antimicrobial agents that are similar to but not overlapping with those to which NorM confers resistance. The significant reduction in norfloxacin accumulation in E. coli cells attributed to NorM expression can be eliminated rapidly by dissipation of the proton gradient ...
... coli resistance to a group of antimicrobial agents that are similar to but not overlapping with those to which NorM confers resistance. The significant reduction in norfloxacin accumulation in E. coli cells attributed to NorM expression can be eliminated rapidly by dissipation of the proton gradient ...
Identification of a Vinyl Reductase Gene for
... genes have been identified in higher plants. However, only the gene for 3,8-divinyl protochlorophyllide a 8-vinyl reductase (DVR), which is indispensable for monovinyl chlorophyll synthesis, has not been identified yet. In this study, we isolated an Arabidopsis thaliana mutant that accumulated divin ...
... genes have been identified in higher plants. However, only the gene for 3,8-divinyl protochlorophyllide a 8-vinyl reductase (DVR), which is indispensable for monovinyl chlorophyll synthesis, has not been identified yet. In this study, we isolated an Arabidopsis thaliana mutant that accumulated divin ...
Capacitation-associated Changes in Protein
... different groups: modified TALP (Tyrode’s albumin lactate pyruvate) or TALP without one of the medium constituents (Ca2+, HCO3- and BSA). After incubation for the required time (0 h, 0.5 h, 1 h, 3 h, 5 h, and 7 h), sperm were removed for further experiment. The capacitation effect was assessed by CT ...
... different groups: modified TALP (Tyrode’s albumin lactate pyruvate) or TALP without one of the medium constituents (Ca2+, HCO3- and BSA). After incubation for the required time (0 h, 0.5 h, 1 h, 3 h, 5 h, and 7 h), sperm were removed for further experiment. The capacitation effect was assessed by CT ...
Effect of Aminoguanidine (Inducible Nitric Oxide Synthase Inhibitor
... protein which is usually a transcription factor. However, in PDKs, the H-Box in N-terminal domain was proved not to be the site of PDK autophosphorylation, since autophosphorylation was mapped to serine residues in this domain [50]. Similarly, the apparent molecular masses of the PDKs determined by ...
... protein which is usually a transcription factor. However, in PDKs, the H-Box in N-terminal domain was proved not to be the site of PDK autophosphorylation, since autophosphorylation was mapped to serine residues in this domain [50]. Similarly, the apparent molecular masses of the PDKs determined by ...
Multiple Functions of the New Cytokine
... be involved in inflammatory responses and immunoregulation. The human tslp gene gives rise to two transcription and translation variants: a long form (lfTSLP) that is induced by inflammation, and a short, constitutively-expressed form (sfTSLP), that appears to be downregulated by inflammation. The T ...
... be involved in inflammatory responses and immunoregulation. The human tslp gene gives rise to two transcription and translation variants: a long form (lfTSLP) that is induced by inflammation, and a short, constitutively-expressed form (sfTSLP), that appears to be downregulated by inflammation. The T ...
attached paper highlights
... Transcription and replication of mitochondrial DNA is driven by the nuclear-encoded mitochondrial transcription factor A (Tfam), which binds to a common upstream enhancer of the promoter sites of the two mitochondrial DNA strands. Cardiac-specific Tfam deletion results in decreased levels of mtDNA, i ...
... Transcription and replication of mitochondrial DNA is driven by the nuclear-encoded mitochondrial transcription factor A (Tfam), which binds to a common upstream enhancer of the promoter sites of the two mitochondrial DNA strands. Cardiac-specific Tfam deletion results in decreased levels of mtDNA, i ...
AngelasPPT
... MOI for 1 hour (to allow one round of infection) Why at high MOI? So each cell can be infected by both mutants am2- ...
... MOI for 1 hour (to allow one round of infection) Why at high MOI? So each cell can be infected by both mutants am2- ...
Dateien anzeigen - Universität Düsseldorf
... metabolism of G. oxydans also poses a problem, which is the low cell yield resulting in high costs for biomass production. This study aimed at an increase of the cell yield of G. oxydans on glucose in order to improve its application potential. For this purpose, prevention of incomplete glucose oxid ...
... metabolism of G. oxydans also poses a problem, which is the low cell yield resulting in high costs for biomass production. This study aimed at an increase of the cell yield of G. oxydans on glucose in order to improve its application potential. For this purpose, prevention of incomplete glucose oxid ...
A systems biology approach sheds new light on the regulation of
... In my years in Birmingham I had the pleasure to meet many friends and co-workers. I would like to thank them for being there to support me and I am sorry if I cannot mention all of them in this page. However, they will always be in my thoughts. The most special thanks are for my supervisor, Dr Franc ...
... In my years in Birmingham I had the pleasure to meet many friends and co-workers. I would like to thank them for being there to support me and I am sorry if I cannot mention all of them in this page. However, they will always be in my thoughts. The most special thanks are for my supervisor, Dr Franc ...
Examining Escherichia coli glycolytic pathways, catabolite
... (Fig. 2c), much higher utilization than in the WT strain. Therefore, the removal of one pivotal EMP enzyme, phosphofructokinase I, alleviated glucose catabolite repression. Acetate, another common component in lignocellulosic hydrolysate, has to be metabolized through gluconeogenesis [20]. ΔpfkA is ...
... (Fig. 2c), much higher utilization than in the WT strain. Therefore, the removal of one pivotal EMP enzyme, phosphofructokinase I, alleviated glucose catabolite repression. Acetate, another common component in lignocellulosic hydrolysate, has to be metabolized through gluconeogenesis [20]. ΔpfkA is ...