DOCTORAL THESIS
... hybrids were attempted in order to design a chimeric enzyme with one or more of the following altered properties: specificity, activity, and or stability. The two enzymes, Bacillus licheniformis 1,3-1,4-β-glucanase and Populus tremula x tremuloides xyloglucan endotransglycosylase, are members of the ...
... hybrids were attempted in order to design a chimeric enzyme with one or more of the following altered properties: specificity, activity, and or stability. The two enzymes, Bacillus licheniformis 1,3-1,4-β-glucanase and Populus tremula x tremuloides xyloglucan endotransglycosylase, are members of the ...
Cytochromes P450 – importance of tissue specificity
... Small amounts of some cytochromes P450 can be translocated to the outer layer of the plasma membrane. The specific content of cytochromes P450 in plasma membrane was determined to be 9% of that in the microsomes as concluded from studies on plasma membrane preparations. [36]. Pulse chase labeling ex ...
... Small amounts of some cytochromes P450 can be translocated to the outer layer of the plasma membrane. The specific content of cytochromes P450 in plasma membrane was determined to be 9% of that in the microsomes as concluded from studies on plasma membrane preparations. [36]. Pulse chase labeling ex ...
sequence-structure relationship - HAL
... carried out when the target sequence shares a good sequence similarity with proteins of known 3D structures (Sali and Blundell, 1993), (ii) threading approach is used when the template structure is hard to find due to a low sequence similarity. It searches for the best compatibility between the targ ...
... carried out when the target sequence shares a good sequence similarity with proteins of known 3D structures (Sali and Blundell, 1993), (ii) threading approach is used when the template structure is hard to find due to a low sequence similarity. It searches for the best compatibility between the targ ...
Decreased expression of plastidial adenylate kinase in potato tubers
... inactive (covalently-linked dimer) protein according to Tiessen et al. (2002). Statistical analysis Data were analysed using Students t test and deemed significant if P ...
... inactive (covalently-linked dimer) protein according to Tiessen et al. (2002). Statistical analysis Data were analysed using Students t test and deemed significant if P ...
(lip) that - Repositories
... transposable element Mu dJ. The chromosomal location of these insertion mutations was determined to be at 14 minutes by bacteriophage P22-mediated cotransduction. The lip gene is transcribed in the clockwise direction with respect to the S. typhimurium genetic map. Strains with lipvAac operon fusion ...
... transposable element Mu dJ. The chromosomal location of these insertion mutations was determined to be at 14 minutes by bacteriophage P22-mediated cotransduction. The lip gene is transcribed in the clockwise direction with respect to the S. typhimurium genetic map. Strains with lipvAac operon fusion ...
Altered pathogenicity, immunogenicity, tissue tropism and 3`
... Abstract: In this study, we attenuated a Chinese LX4-type nephropathogenic infectious bronchitis virus (IBV) strain, CK/CH/LHLJ/04V, by serial passage in embryonated chicken eggs. Based on sequence analysis of the 3'-7 kb region, the CK/CH/LHLJ/04V virus population contained subpopulations with a mi ...
... Abstract: In this study, we attenuated a Chinese LX4-type nephropathogenic infectious bronchitis virus (IBV) strain, CK/CH/LHLJ/04V, by serial passage in embryonated chicken eggs. Based on sequence analysis of the 3'-7 kb region, the CK/CH/LHLJ/04V virus population contained subpopulations with a mi ...
Identification and Analysis of Dicer Associated Proteins in
... mechanism of miRNA-mediated repression. Different models were proposed, supporting the idea of translational repression either at the level of initiation or at a post-initiation level. In addition, there is evidence for miRNA-induced mRNA degradation that occurs independently of Agos’ RNase H activi ...
... mechanism of miRNA-mediated repression. Different models were proposed, supporting the idea of translational repression either at the level of initiation or at a post-initiation level. In addition, there is evidence for miRNA-induced mRNA degradation that occurs independently of Agos’ RNase H activi ...
Engineering subunit association of multisubunit proteins
... barrel surfaces have complementary curvatures, making numerous intersubunit van der Waals and electrostatic interactions. The tetramer is formed by the association of two such stable dimers, which are connected relatively weakly across a small intersubunit contact area. The difference in calculated ...
... barrel surfaces have complementary curvatures, making numerous intersubunit van der Waals and electrostatic interactions. The tetramer is formed by the association of two such stable dimers, which are connected relatively weakly across a small intersubunit contact area. The difference in calculated ...
Candida antarctica Anders G. Sandström
... out an anaerobic oxidation of carbohydrates to form ethanol. Saccharomyces cerevisiae was used by the Bayer corporation already in the 1930‟s to form a precursor to ephedrine, L-phenylacetylcarbinol via whole-cell biotransformation of benzaldehyde.11 Ever since then, the use of enzymes for biotransf ...
... out an anaerobic oxidation of carbohydrates to form ethanol. Saccharomyces cerevisiae was used by the Bayer corporation already in the 1930‟s to form a precursor to ephedrine, L-phenylacetylcarbinol via whole-cell biotransformation of benzaldehyde.11 Ever since then, the use of enzymes for biotransf ...
Alpha and beta subunits of pyruvate dehydrogenase E1
... phylogenies including microsporidian data [6– 8], however, it is now clear that this is not the case, and that microsporidia are instead highly derived and extremely divergent fungi. This fungal origin of microsporidia was first proposed based on the relationship between microsporidia and fungi in t ...
... phylogenies including microsporidian data [6– 8], however, it is now clear that this is not the case, and that microsporidia are instead highly derived and extremely divergent fungi. This fungal origin of microsporidia was first proposed based on the relationship between microsporidia and fungi in t ...
Microbiology 146:
... The isolation and characterization of a Rhizobium etli glutamate auxotroph, TAD12, harbouring a single Tn5 insertion, is reported. This mutant produced no detectable glutamate synthase (GOGAT) activity. The cloning and physical characterization of a 72 kb fragment of R. etli DNA harbouring the stru ...
... The isolation and characterization of a Rhizobium etli glutamate auxotroph, TAD12, harbouring a single Tn5 insertion, is reported. This mutant produced no detectable glutamate synthase (GOGAT) activity. The cloning and physical characterization of a 72 kb fragment of R. etli DNA harbouring the stru ...
Genetic Engineering of Field, Industrial and Pharmaceutical Crops
... Techniques such as genetic transformation have opened new vistas whereby functional genes, not commonly present in a particular species can be added from other species. The traits incorporated into the genetically engineered plants in the beginning were confined to those governed by dominant genes, ...
... Techniques such as genetic transformation have opened new vistas whereby functional genes, not commonly present in a particular species can be added from other species. The traits incorporated into the genetically engineered plants in the beginning were confined to those governed by dominant genes, ...
... repressor protein from a high DNA affinity (R) state to a low DNA affinity T state, this causes the production of enzymes to digest lactose. 9. (15 pts) What features of the active site of an enzyme leads to an increase in the rate of the chemical reaction and specificity towards substrates. Illustr ...
Goal - Cytoscape Wiki
... • Active modules – Input: network + p-values for gene expression values e.g. from GCRMA – Output: significantly differentially expressed subgraphs ...
... • Active modules – Input: network + p-values for gene expression values e.g. from GCRMA – Output: significantly differentially expressed subgraphs ...
A non-canonical pathway for aromatic amino acid biosynthesis in
... Large scale purification of OE1472F and OE1477R .............................. 112 ...
... Large scale purification of OE1472F and OE1477R .............................. 112 ...
NIH Public Access - University of Utah Health Care
... As part of an ongoing effort to characterize mitochondrial proteins that are conserved through evolution, we initiated studies of the MPC protein family (originally designated BRP44 and BRP44L in humans) (8). This family contains three members in Saccharomyces cerevisiae, encoded by YGL080W, YHR162W ...
... As part of an ongoing effort to characterize mitochondrial proteins that are conserved through evolution, we initiated studies of the MPC protein family (originally designated BRP44 and BRP44L in humans) (8). This family contains three members in Saccharomyces cerevisiae, encoded by YGL080W, YHR162W ...
02_whole - Massey Research Online
... restriction sites are unique to each individual MD-AeO sequence. Genomic Southern analysis, using probes spanning the 3 '-UTR and the 3 '-end of the coding region confirmed that MD A e03 is encoded by a distinct gene. However, while the distinction between MD-A eO] and MD-A e02 is not as definitive ...
... restriction sites are unique to each individual MD-AeO sequence. Genomic Southern analysis, using probes spanning the 3 '-UTR and the 3 '-end of the coding region confirmed that MD A e03 is encoded by a distinct gene. However, while the distinction between MD-A eO] and MD-A e02 is not as definitive ...
Possible plant mitochondria involvement in cell
... stresses may be associated with efficient defence against oxidative stress at cellular and subcellular levels. To do this, plant mitochondria display three different strategies (Møller, 2001): the first line of defence is the avoidance of ROS production, achieved by keeping the electron transport ch ...
... stresses may be associated with efficient defence against oxidative stress at cellular and subcellular levels. To do this, plant mitochondria display three different strategies (Møller, 2001): the first line of defence is the avoidance of ROS production, achieved by keeping the electron transport ch ...
Exploring Tomato Gene Functions Based on Coexpression Modules
... (Fukushima et al., 2009; Stitt et al., 2010). Large-scale data from genome-wide gene expression profiling with DNA microarrays are publicly available for many species, including Arabidopsis thaliana, Oryza sativa (rice), poplar, and some crops (Ogata et al., 2010; Tohge and Fernie, 2010). These data ...
... (Fukushima et al., 2009; Stitt et al., 2010). Large-scale data from genome-wide gene expression profiling with DNA microarrays are publicly available for many species, including Arabidopsis thaliana, Oryza sativa (rice), poplar, and some crops (Ogata et al., 2010; Tohge and Fernie, 2010). These data ...
Genetic tools for manipulating Acinetobacter baumannii genome: an
... as a temperature-sensitive replicon. The vector must also carry a selectable marker, usually an antibiotic resistance gene. Sometimes integration vectors also contain oriT so that the construct can be transferred to other bacteria by conjugation. This is especially helpful when the other transfer me ...
... as a temperature-sensitive replicon. The vector must also carry a selectable marker, usually an antibiotic resistance gene. Sometimes integration vectors also contain oriT so that the construct can be transferred to other bacteria by conjugation. This is especially helpful when the other transfer me ...
ISOLATION AND FUNCTIONAL GENETIC EUCALYPTUS Magister Scientiae
... Eucalyptus is an important genus of forest tree species which are widely used as a resource for wood and fibre for the pulp and papermaking industry. However, the yield of wood and fibre of Eucalyptus and other woody species is not high enough to meet the world-wide demand. The pulp and papermaking ...
... Eucalyptus is an important genus of forest tree species which are widely used as a resource for wood and fibre for the pulp and papermaking industry. However, the yield of wood and fibre of Eucalyptus and other woody species is not high enough to meet the world-wide demand. The pulp and papermaking ...
AMP-activated protein kinase phosphorylates transcription factors of
... CREB phosphorylation was lower in gastrocnemius, tibialis anterior, and heart muscles (Fig. 2) from a separate group of MLKB1-KO mice (in which AMPK phosphorylation is virtually eliminated), suggesting that AMPK may play a role in maintaining basal levels of CREB phosphorylation. CREB phosphorylatio ...
... CREB phosphorylation was lower in gastrocnemius, tibialis anterior, and heart muscles (Fig. 2) from a separate group of MLKB1-KO mice (in which AMPK phosphorylation is virtually eliminated), suggesting that AMPK may play a role in maintaining basal levels of CREB phosphorylation. CREB phosphorylatio ...
Protein-protein interactions: mechanisms and
... The charge density varies from 0 to 12 charged groups per interface surface (Xu et al., 1997b). The distribution of the opposite charges in the interfaces of the contacting area showed that salt bridges across them are highly favourable (Drozdov-Tikhomirov et al., 2001; Xu et al., 1997a,b). The deso ...
... The charge density varies from 0 to 12 charged groups per interface surface (Xu et al., 1997b). The distribution of the opposite charges in the interfaces of the contacting area showed that salt bridges across them are highly favourable (Drozdov-Tikhomirov et al., 2001; Xu et al., 1997a,b). The deso ...
In Silico Prediction of the Peroxisomal Proteome in Fungi, Plants
... requires around 20 PEX genes and their products, the peroxins.8 PTS1 interacts with the tetratricopeptide repeats (TPRs) of the receptor Pex5p.16 Proteins bearing PTS2 bind to the WD40 motifs of Pex7p.17 After binding of their cargo proteins, these receptors are thought to interact with a docking co ...
... requires around 20 PEX genes and their products, the peroxins.8 PTS1 interacts with the tetratricopeptide repeats (TPRs) of the receptor Pex5p.16 Proteins bearing PTS2 bind to the WD40 motifs of Pex7p.17 After binding of their cargo proteins, these receptors are thought to interact with a docking co ...
Annotation guidelines - Systems Biology and Bioinformatics
... (i) Two polytopic membrane proteins, NarK and NarU, are assumed to transport nitrite out of the Escherichia coli cytoplasm. Examples of (h) and (i) contain words that can potentially be GP, vitamin B6-enzyme in (h) and Two polytopic membrane proteins in (i). In this particular case, both are not ann ...
... (i) Two polytopic membrane proteins, NarK and NarU, are assumed to transport nitrite out of the Escherichia coli cytoplasm. Examples of (h) and (i) contain words that can potentially be GP, vitamin B6-enzyme in (h) and Two polytopic membrane proteins in (i). In this particular case, both are not ann ...