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Ethylene is involved in strawberry fruit ripening in an organ
Ethylene is involved in strawberry fruit ripening in an organ

... Strawberry leaf discs were transformed with Agrobacterium tumefaciens LBA4404 carrying a pCD-2 plasmid that contained the kanamycin resistance gene nptII and the cDNA for the mutant allele of etr1-1 (At1g66340) in the sense orientation under the control of a single constitutive [Cauliflower mosiac v ...
Evolution of Function in Protein Superfamilies, from a Structural
Evolution of Function in Protein Superfamilies, from a Structural

... The recent growth in protein databases has revealed the functional diversity of many protein superfamilies. We have assessed the functional variation of homologous enzyme superfamilies containing two or more enzymes, as de®ned by the CATH protein structure classi®cation, by way of the Enzyme Commiss ...
Eds., M. Kawaguchi, K. Misaki, H. Sato, T. Yokokawa, T.... and S. Tanabe, pp. 35–40.
Eds., M. Kawaguchi, K. Misaki, H. Sato, T. Yokokawa, T.... and S. Tanabe, pp. 35–40.

... Fig. 1. Schematic illustration of the effect of taurine for protecting germ cell from oxidative stress by promoting Cu/Zn SOD expression. O 2–, reactive oxygen species. ...
Moore BA, Gonzalez Aviles GD, Larkins CE, Hillman MJ, Caspary T. Mamm Genome. 2010 Aug;21(7-8):350-60. Mitochondrial retention of Opa1 is required for mouse embryogenesis
Moore BA, Gonzalez Aviles GD, Larkins CE, Hillman MJ, Caspary T. Mamm Genome. 2010 Aug;21(7-8):350-60. Mitochondrial retention of Opa1 is required for mouse embryogenesis

... Fig. 3 Mislocalization of Opa1 protein in lilR3 embryos. Western blot analysis of mitochondrial (left panels) and cytosolic (right panels) E10.5 embryo protein lysates using antibodies against Opa1, a-tubulin (loading control), and cytochrome c (mitochondrial marker, demonstrating the efficiency of ...
Sequence - BIOTEC - Biotechnology Center TU Dresden
Sequence - BIOTEC - Biotechnology Center TU Dresden

...  forward (deliberate modification of biological systems) ...
PDF Print - APS Journals
PDF Print - APS Journals

... predict that RuBisCO is likely used in a putative metabolic pathway to supplement carbon obtained heterotrophically with low-cost assimilation of carbon from CO2. We validate results of the comparative analysis by predicting correct phenotype, pathogenic or mutualistic, for 20 symbionts in an indepe ...
ADVANCES IN AGRICULTURAL SCIENCES XIV (1–2)
ADVANCES IN AGRICULTURAL SCIENCES XIV (1–2)

... the duration of low-calorie diet. Another considered method of impacting on the youth of the immunological system involves application of hormones and cytokines (Dorshkind et al. 2009). The main objective of this type of treatment is to rejuvenate the disappearing thymus, as well as many cells of th ...
Pineau B, Bourge M, Marion J, Mauve C, Gilard F, Maneta
Pineau B, Bourge M, Marion J, Mauve C, Gilard F, Maneta

... their association with CL in the plant kingdom is unclear. Here, we characterize three mutants disrupted in the Arabidopsis CLS single-copy gene, named cls mutants. In vitro, germinating seedlings of cls mutants are able to develop under low-illumination conditions only, and all three have a slow-gr ...
Biosynthesis of branched-chain amino acids is
Biosynthesis of branched-chain amino acids is

... was not auxotrophic for BCAAs and could grow well on minimal medium with pyruvate as a carbon source and ammonia as a nitrogen source. However, it grew less efficiently than the wildtype (WT) strain when ammonia was substituted with valine or isoleucine as a nitrogen source. The BCAA aminotransferas ...
The Role of the Bundle Sheath in the Leaf Development of C3 plants
The Role of the Bundle Sheath in the Leaf Development of C3 plants

... development of the inner leaf architecture. These mutants are aberrant in the mesophyll (M) but have an intact vasculature and bundle sheath (BS). While the role of the BS in C 4 plants is well established, knowledge about the BS in C3 plants is scarce. Reticulate mutants are defective in primary me ...
Multivariate Modeling of Proteins Related to
Multivariate Modeling of Proteins Related to

... was used to test the significance of the components. Hence, this validation technique increases the stability of the results. Such validation is not implemented in other common statistical packages e.g., SPSS. The PLS-DA model was validated by both response permutation tests and CV-ANOVA on the resi ...
Pentose phosphates in nucleoside interconversion and catabolism
Pentose phosphates in nucleoside interconversion and catabolism

... degrade or synthesize deoxyribonucleosides phosphorolytically. As they also often have a growth requirement for deoxynucleosides, it is important that these compounds are not degraded when present in the medium. The presence of the N-deoxyribosyltransferase and all four nucleobases found in DNA and ...
Nutrition, Anabolism, and the Wound Healing Process: An Overview
Nutrition, Anabolism, and the Wound Healing Process: An Overview

... and other hormones that produce a hypermetabolic-catabolic state.38–41 An entire spectrum of abnormalities can be seen after injury and inflammation due to degrees of the manifestation of the host “stress response” to a body wound. If uncontrolled, the stress response can progress with loss of body ...
Soy Protein Based Green Composite: A Review
Soy Protein Based Green Composite: A Review

... near its isoelectric point (pH 4.5) due to the coagulation. However, proteins can be denatured and unfolded at pH values away from their isoelectric point, thus exposing their functional groups and increasing the intermolecular interactions. Therefore the mechanical properties of soy protein can be ...
Regulation of Cytochrome bd Expression in Mycobacterium
Regulation of Cytochrome bd Expression in Mycobacterium

... Mycobacteria are Gram-positive and acid-fast bacteria, belonging to the phylum of Actinobacteria. Mycobacteria possess G + C rich genomes with a content of about 62-70% (24). They are considered to be obligate aerobes and have a rod-shaped appearance of about 0.3 - 0.5 µm in diameter and of variable ...
regulation of mammalian acetyl
regulation of mammalian acetyl

... by cAMP-dependent protein kinase and ACC kinase (67) causes primarily an increase in the Ka for citrate and a more modest decrease in Vmax (67). The isolation and characterization of the various phosphopeptides of ACC-α that can be phosphorylated by different protein kinases in vitro and in vivo (32 ...
SPECIFIC PROTEIN SYNTHESIS IN CELLULAR
SPECIFIC PROTEIN SYNTHESIS IN CELLULAR

... laterally so as to form voluminous channels for yolk transport (King and Aggarwal, 1965) . In addition, the cells synthesize and secrete into the channels a macromolecule, probably a glycoprotein, which finds its way into the yolk spheres of the oocyte (Anderson and Telfer, 1969) . From experiments ...
and gender-specific hallmarks
and gender-specific hallmarks

... addressed. Most of the studies have largely relied on a variety of experimental models of obesity and cultured adipocytes, and most of them have resorted to electrophoretic protein separation, which has very limited proteome coverage and results in a poor representation of low-abundant or very hydro ...
Characterization of cytochrome P450
Characterization of cytochrome P450

... Full length isolated CYPs were cloned at EcoRI and NotI restriction sites in MCS1 of pESC-URA containing a CPR (JN594507) sequence amplified from A. annua at BamHI and SalI restriction enzyme sites of MCS2 (Ro et al., 2006). List of primers used for full length gene amplification and cloning in yeast ...
Database Searching for Protein Identification and
Database Searching for Protein Identification and

... proteins, e.g. mRNAs. In most cases, you will not get satisfactory results from an EST database, where most of the entries correspond to protein fragments, or genomic DNA, where there is a continuum of sequence, containing regions coding for multiple proteins as well as non-coding regions. This is b ...
Sun J, Ke J, Johnson JL, Nikolau BJ, Wurtele ES
Sun J, Ke J, Johnson JL, Nikolau BJ, Wurtele ES

... The cDNA clone 150M20T7 was identified in the Arabidopsis collection of EST clones (Newman et al., 1994) because it codes for a peptide that shows a high degree of sequence similarity to the biotin carboxylase of E. coli and Anabaena sp. strain PCC 7120 (Li and Cronan, 1992a, 1992b; Gornicki et al., ...
EFFECTS OF INSULIN DEFICIENCY ON EXERCISE
EFFECTS OF INSULIN DEFICIENCY ON EXERCISE

... the main nutrients. Its main function is to stimulate glucose uptake and disposal or utilization by the cells and thus to decrease blood glucose concentration. However, it also inhibits breakdown of proteins and lipids and promotes their synthesis. Type 1 diabetes is a disease in which insulin secre ...


... correct order of the CNBr fragments, e.g. 1-2-3. Justify your approach on the back of the previous page. 1 – 3 – 2. (4 pts for correct answer) CNBr 1 is the first fragment since its sequence agrees with the amino-terminal sequence of the peptide. The third CNBr fragment has an Arginine, which is a c ...
Text - Enlighten: Publications
Text - Enlighten: Publications

... continue for between seven to eight weeks [4] and thereafter, up to 85% of individuals develop a chronic infection. Over a period of decades, chronic infection can lead to severe liver disease [5], including decompensated cirrhosis and hepatocellular carcinoma, which are end-stage conditions [6]. 1. ...
The Principles of Balancing Diets for Amino Acids and Their Impact
The Principles of Balancing Diets for Amino Acids and Their Impact

... rumen bacteria or in milk and tissue protein (Table 1). Many nutritionists have questioned the possibility of histidine (His) as a potential limiting AA. However, to the author’s knowledge, His has only been identified as first limiting when grass silage and barley and oat diets were fed, with or wi ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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