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Characterization of the binding properties of the Avian Coronavirus
Characterization of the binding properties of the Avian Coronavirus

... Measurement of DNA-concentration ..................................................... 42 ...
Mini-Series: Modern Metabolic Concepts The Biochemistry of the
Mini-Series: Modern Metabolic Concepts The Biochemistry of the

... for the A. vinelandii E1 was shown to involve not only the subunit-binding domain but also the E2 inner domain [9]. PDK is a specific kinase that phosphorylates E1 of PDC and is present in human, rodent, plant, nematode, and fruit fly. PDK has several isoforms, four in mammalian PDC (PDK1, PDK2, PDK ...
Full Text  - the American Society of Animal Science
Full Text - the American Society of Animal Science

... P = 0.75), mRNA levels of sterol-regulatory element binding protein-1 (P = 0.6), or diacylglycerol acyltransferase 1 (P = 0.6). Adipocyte fatty acid binding protein (FABP)-4 protein content in whole LM was 2-fold greater in HF pigs than in LF pigs (P = 0.05), and positive correlation coefficients we ...
The exam is worth 200 points, divided into 7 questions. You must do
The exam is worth 200 points, divided into 7 questions. You must do

... 1. Nucleic acid structure and hydrogen bonding (35 pts) Triple-helical DNA has been proposed as a possible highly-selective therapeutic agent for the control of gene expression. The formation of triple-helical DNA relies on specific hydrogen bonds formed between the third strand and the major groove ...
Lo, Frederick: Three Recently Developed Algorithms for Aligning Distantly Related Proteins
Lo, Frederick: Three Recently Developed Algorithms for Aligning Distantly Related Proteins

... some differences. The sequence-sequence similarity matrix can apparently be constructed from a substitution matrix and dynamic programming. Like an amino-acid substitution matrix, the similarity matrix measures similarity between pairs of query and template residues. However, while a high value in ...
MS-Word format
MS-Word format

... the children terms of seed (PO:0009010). Also explain why this number is not the same as you see next to the term seed (PO:0009010). Hint: There are two types of associations in the ontology: direct associations, which are directly associated to the term for which a phenotype has been characterized ...
Bioinformatics
Bioinformatics

... 3. DNA DataBank of Japan (DDBJ) at Mishima, Japan http://www.ddbj.nig.ac.jp/ 4. Protein International Resource (PIR) database at the National Biomedical Research Foundation in Washington, DC (see Barker et al. 1998) http://wwwnbrf.georgetown.edu/pirwww/ 5. The SwissProt protein sequence database at ...
File S4 – Novel OR alleles in the CAST genome, Related
File S4 – Novel OR alleles in the CAST genome, Related

Metabolic flux profiling of recombinant protein secreting Pichia
Metabolic flux profiling of recombinant protein secreting Pichia

... The AOX1 promoter of P. pastoris has been widely used for recombinant protein production. The conceptual basis for this expression system stems from the observation that some of the enzymes required for methanol metabolism are present at substantial levels only when cells are grown on this substrate ...
Supporting document 1 Safety assessment
Supporting document 1 Safety assessment

... but the mature form of protein is slightly larger (by 34 amino acids) than anticipated due to the N-terminal signal peptide being cleaved at a different site to what had been previously predicted. The AHAS protein expressed in CV127 is not glycosylated and exhibits the expected enzymatic activity. A ...
Substrate specificity of the periplasmic dipeptide
Substrate specificity of the periplasmic dipeptide

... progressively enhanced by alkylation (with methyl to butyl) of the N-terminal α-amino group. Thus, results from this approach provide an essential experimental basis, which complements the information from the crystal structure of DppA, for the design of peptidomimetic antibacterials targeted for tr ...
Metabolomics based gene function annotation in Escherichia coli
Metabolomics based gene function annotation in Escherichia coli

... mutants. Additionally, metabolomics can be used to explore metabolic diversity of different accessions/strains, tissues, and cell types of an organism. Once the metabolic diversity has been described, the genetic-basis for the metabolic and/or phenotypic differences can be elucidated using other omi ...
Mutational analysis of exoribonuclease I from Saccharomyces
Mutational analysis of exoribonuclease I from Saccharomyces

... and its complementary oligonucleotide were ligated into the AatII and HindIII sites of pRDK249. A series of nested C-terminal deletions of XRN1 in pUC118 (kindly provided by P.Ljungdahl) was digested with HindIII and SstI and the XRN1 mutant-bearing fragments were ligated into HindIII and SstI-diges ...
Host Factors in the Replication of Positive
Host Factors in the Replication of Positive

... Following translation and processing of viral proteins, (+)RNA viruses adopt a relatively conserved process for completing the RNA replication cycle in host cells. Studies of the mechanisms of (+)RNA virus replication have suggested that this process usually involves the following steps: 1) selectin ...
Metabolic decision making by protein-metabolite - ETH E
Metabolic decision making by protein-metabolite - ETH E

... power, and energy. To regulate metabolic activity, microbes utilize a complex network of regulatory  layers,  such  as  transcriptional  regulation,  covalent  posttranslational  modifications,  and  allostery.  Importantly, metabolism is not merely the endpoint of regulation, but can also provide f ...
Lecture 6 - Andrew.cmu.edu
Lecture 6 - Andrew.cmu.edu

... last three residues (Ala-Phe-Leu )could not be determined due to limitation in the Edman degradation to 7 steps, these are highlighted gray). Ala ...
Yeast Nutrients Make Fermentations Better
Yeast Nutrients Make Fermentations Better

... nitrogen is provided in the form of amino acids. To supplement nitrogen, ammonium sulfate is commonly used. A preferred source however, is amino acids. Yeast grow better on amino acids because amino acids are the building blocks of proteins. There are 20 different types of amino acids, and yeast can ...
Glucose Polyester Biosynthesis. Purification and
Glucose Polyester Biosynthesis. Purification and

... relatively high. Thus, the 108% yield was most likely caused by variability in measurement rather than being an indication of the existence of inhibitors. DEAE chromatography separated the activity from the bulk of the protein and resulted in an approximately 5-fold increase in specific activity (Fi ...
Arginine-induced stimulation of protein synthesis and survival in
Arginine-induced stimulation of protein synthesis and survival in

... to synthesize arginine (20, 37, 41), and the small intestine is a key site of net arginine synthesis in neonates (39, 42, 43). Thus the gut appears to play a critical role in maintaining arginine homeostasis in neonates (13, 40). Evidence from in vitro metabolic studies with primary cells and transf ...
University of Groningen Cross-linking of dimeric CitS and GltS
University of Groningen Cross-linking of dimeric CitS and GltS

... and includes the Ion Transporter (IT) superfamily ...
Protein Sequences in the CAS Registry File on
Protein Sequences in the CAS Registry File on

... REGISTRY on STN. References to protein sequences may be searched and displayed in bibliographic files on STN, e.g., CAplusSM. Protein sequence information in REGISTRY is compiled by CAS from sequences reported in research articles and patents. Sequences may be found in REGISTRY for the following cla ...
The Role of Nucleoside Diphosphate Kinase in Plant Mitochondria
The Role of Nucleoside Diphosphate Kinase in Plant Mitochondria

... nucleoside triphosphate pools. It has become recognized that as well as having the kinase activity, NDPK proteins have additional or different roles. This study concerns an NDPK isoform located in plant mitochondria that supports the eversurprising functions of the protein. There is increasing evide ...
Pan-cancer analysis of the metabolic reaction network
Pan-cancer analysis of the metabolic reaction network

... metabolic phenotypes associated with cancer questions the extent to which these regulatory ...
Molecular characteristics of sucrose synthase
Molecular characteristics of sucrose synthase

... technique under denaturing conditions. This method allowed to separate and reveal SuSy subunits within polyacrylamide gel. Electropherograms of the analysed isoenzymes isolated from bird cherry tissues indicated that their structure was probable composed of three subunits. Published data referring t ...
BIOL 1322 General Nutrition
BIOL 1322 General Nutrition

... Understand celiac disease, GERD, IBS, ulcerative colitis, Crohns, peptic ulcer, etc and be able to distinguish between and among Identify where bile is formed, stored and its function Identify where bicarbonate is formed and released and its function Identify the major organs/sphincters/accessory or ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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