Modifying the chain-length selectivity of the
... 1999). High throughput of mutant proteins can be investigated efficiently by the in vitro method because it is not necessary to clone the mutant genes. Compared with the traditional in vivo protein engineering method, the advantage of the in vitro system is obvious. In this work, we applied the meth ...
... 1999). High throughput of mutant proteins can be investigated efficiently by the in vitro method because it is not necessary to clone the mutant genes. Compared with the traditional in vivo protein engineering method, the advantage of the in vitro system is obvious. In this work, we applied the meth ...
PDF of original
... markedly the binding of C14-Phe - and C14-LyssRNA, respectively. Such data directly demonstrate a triplet code and also show that codons contain three sequential bases. The template activity of triplets with 5'-terminal phosphate, pUpUpU, equals that of the corresponding tetra- and pentanucleotides; ...
... markedly the binding of C14-Phe - and C14-LyssRNA, respectively. Such data directly demonstrate a triplet code and also show that codons contain three sequential bases. The template activity of triplets with 5'-terminal phosphate, pUpUpU, equals that of the corresponding tetra- and pentanucleotides; ...
Protein Sorting and Transport - The Endoplasmic Reticulum, Golgi
... chain. The ribosome then binds to a protein translocation complex in the ER membrane, and the signal sequence is inserted into a membrane channel. In both yeast and mammalian cells, the translocation channels through the ER membrane are complexes of three transmembrane proteins, called the Sec61 pro ...
... chain. The ribosome then binds to a protein translocation complex in the ER membrane, and the signal sequence is inserted into a membrane channel. In both yeast and mammalian cells, the translocation channels through the ER membrane are complexes of three transmembrane proteins, called the Sec61 pro ...
review on enhancement of glucose uptake and up
... Glucose is a key fuel in mammalian cells that import by a process of facilitative diffusion mediated by glucose transporters (GLUT). A defect in GLUT expression for prolong time leads to diabetes mellitus. Medicinal plants used in traditional treatments confirm a possibility of tackling diabetes by ...
... Glucose is a key fuel in mammalian cells that import by a process of facilitative diffusion mediated by glucose transporters (GLUT). A defect in GLUT expression for prolong time leads to diabetes mellitus. Medicinal plants used in traditional treatments confirm a possibility of tackling diabetes by ...
Molecular Genetics and Genomics
... The nucleotide and amino acid sequences were analyzed using the BESTFIT program of the Genetics Computer Group (GCG) program package of the University of Wisconsin (Devereux et al. 1984). ...
... The nucleotide and amino acid sequences were analyzed using the BESTFIT program of the Genetics Computer Group (GCG) program package of the University of Wisconsin (Devereux et al. 1984). ...
the structure and function of cartilage proteoglycans
... articulate in movable joints. It is not clear whether articular cartilage can be defined as a distinct tissue in the juvenile, as it forms a continuum with the hyaline cartilage of the central epiphysis, which is destined to be resorbed. Indeed, it is still somewhat of a mystery why articular cartil ...
... articulate in movable joints. It is not clear whether articular cartilage can be defined as a distinct tissue in the juvenile, as it forms a continuum with the hyaline cartilage of the central epiphysis, which is destined to be resorbed. Indeed, it is still somewhat of a mystery why articular cartil ...
Computational protein design enables a novel one
... Computational Design of a Formose Catalyst. Next, computational protein design was used to redesign the BzALD binding pocket of BAL to increase specificity and activity for FALD and the formose reaction. FALD molecules—much smaller than the native ligand BzALD (Fig. 2A and SI Appendix, Fig. S3)—were ...
... Computational Design of a Formose Catalyst. Next, computational protein design was used to redesign the BzALD binding pocket of BAL to increase specificity and activity for FALD and the formose reaction. FALD molecules—much smaller than the native ligand BzALD (Fig. 2A and SI Appendix, Fig. S3)—were ...
Arabidopsis 3-ketoacyl-CoA synthase 9 is involved in the synthesis
... Spatial and temporal expression of KCS9 in Arabidopsis To confirm the relative expression level of the KCS9 gene in different organs or tissues, total RNAs were isolated from 2-week-old roots, 2-week-old seedlings, and rosette leaves, cauline leaves, open flowers, pollen grains, and siliques from 6- ...
... Spatial and temporal expression of KCS9 in Arabidopsis To confirm the relative expression level of the KCS9 gene in different organs or tissues, total RNAs were isolated from 2-week-old roots, 2-week-old seedlings, and rosette leaves, cauline leaves, open flowers, pollen grains, and siliques from 6- ...
Function and biotechnology of extremophilic enzymes in low water
... extreme conditions. The parallel development of sophisticated molecular biology tools has also enabled engineering of enzymes with novel properties using techniques such as site-directed mutagenesis, gene shuffling, directed evolution, chemical modifications and immobilization [4-6]. Microorganisms ...
... extreme conditions. The parallel development of sophisticated molecular biology tools has also enabled engineering of enzymes with novel properties using techniques such as site-directed mutagenesis, gene shuffling, directed evolution, chemical modifications and immobilization [4-6]. Microorganisms ...
PSI
... PolyQ domains can expand and contract PolyQ often serve to mediate interaction between proteins Expansion of polyQ stretches can result in toxicity of corresponding proteins while their contraction may inhibit biological function of these proteins Insertion of non-Q/N amino acid residues int ...
... PolyQ domains can expand and contract PolyQ often serve to mediate interaction between proteins Expansion of polyQ stretches can result in toxicity of corresponding proteins while their contraction may inhibit biological function of these proteins Insertion of non-Q/N amino acid residues int ...
Matrix-assisted Laser Desorption/Ionization Mass Spectrometry in
... pressure pulse theory.7/ proposes that a pressure gradient is created normal to the surface and desorption of large molecules might be enhanced by momentum transfer from collisions with fast-moving matrix molecules. It is generally thought that ionization occurs through proton transfer or cationizat ...
... pressure pulse theory.7/ proposes that a pressure gradient is created normal to the surface and desorption of large molecules might be enhanced by momentum transfer from collisions with fast-moving matrix molecules. It is generally thought that ionization occurs through proton transfer or cationizat ...
The phosphopantetheinyl transferases
... appeared to reside in the cytosol, and the PPant attached to apoACP before holo-ACP translocated into the chloroplast.9 In 1995, Lambalot and Walsh puried AcpS from E. coli 70 000-fold. Curiously, although they achieved a 70 000-fold purication, the protein was still not homogeneous, suggesting th ...
... appeared to reside in the cytosol, and the PPant attached to apoACP before holo-ACP translocated into the chloroplast.9 In 1995, Lambalot and Walsh puried AcpS from E. coli 70 000-fold. Curiously, although they achieved a 70 000-fold purication, the protein was still not homogeneous, suggesting th ...
ppt
... Lets Jump Ahead and Look at Protein Folding Folding Simple Dynamic Simulation.html http://intro.bio.umb.edu/111-112/111F98Lect/folding.html ...
... Lets Jump Ahead and Look at Protein Folding Folding Simple Dynamic Simulation.html http://intro.bio.umb.edu/111-112/111F98Lect/folding.html ...
Protein sequence comparisons show that the
... later 'pseudoproteases' (2); the latter term is used in this paper, as a convenient label only. A model was proposed by which the pseudoprotease coding sequence could have been transferred from the oncovirus lineage to the lentivirus lineage (1). ...
... later 'pseudoproteases' (2); the latter term is used in this paper, as a convenient label only. A model was proposed by which the pseudoprotease coding sequence could have been transferred from the oncovirus lineage to the lentivirus lineage (1). ...
Nutrition to Support Recovery from Endurance Exercise: Optimal
... Carbohydrate intake is crucial to enhance muscle and liver glycogen stores after high-intensity, carbohydratebased exercise. The rate of muscle glycogen resynthesis is generally greatest during the initial È1 h after exercise and is facilitated by the contraction-induced recruitment of GLUT-4 transp ...
... Carbohydrate intake is crucial to enhance muscle and liver glycogen stores after high-intensity, carbohydratebased exercise. The rate of muscle glycogen resynthesis is generally greatest during the initial È1 h after exercise and is facilitated by the contraction-induced recruitment of GLUT-4 transp ...
FOG-1 - Blood Journal
... of early erythroid progenitors but is required for maintenance or maturation of erythroid cells. To quantify our data, the embryos were placed in 3 different categories (normal, reduced, and absent), depending on the level of hemoglobinization or expression for band3 and cd41 in erythrocytes and thr ...
... of early erythroid progenitors but is required for maintenance or maturation of erythroid cells. To quantify our data, the embryos were placed in 3 different categories (normal, reduced, and absent), depending on the level of hemoglobinization or expression for band3 and cd41 in erythrocytes and thr ...
Intrinsically Disordered Protein - Center for Data Analytics and
... albumin’s structural ensemble. Karush called this phenomenon configurational adaptability. Studies on albumin binding to the present support Karush’s original proposal of an ensemble of structures, but with some added and interesting twists such as the existence of different conformers with slow rat ...
... albumin’s structural ensemble. Karush called this phenomenon configurational adaptability. Studies on albumin binding to the present support Karush’s original proposal of an ensemble of structures, but with some added and interesting twists such as the existence of different conformers with slow rat ...
The role of calcium and other ions in sorting and delivery in the late
... mammalian cells, complementary data have been obtained for homotypic fusion of early endosomes [24] and heterotypic fusion of late endosomes with lysosomes [20]. In both systems, fusion was inhibited by BAPTA but not EGTA and was also prevented by pre-incubation with a membranepermeable ester, EGTA- ...
... mammalian cells, complementary data have been obtained for homotypic fusion of early endosomes [24] and heterotypic fusion of late endosomes with lysosomes [20]. In both systems, fusion was inhibited by BAPTA but not EGTA and was also prevented by pre-incubation with a membranepermeable ester, EGTA- ...
Detoxification mechanisms (Apis mellifera) resulting in tolerance of dietary nicotine
... P450-mediated pathway, supporting the notion that the C-oxidation of nicotine by honey bees constitutes true detoxification. In the nicotine-resistant peach-potato aphid (M. persicae) overexpression of a cytochrome P450 (CYP6CY3) allows these insects to efficiently detoxify nicotine to cotinine and ...
... P450-mediated pathway, supporting the notion that the C-oxidation of nicotine by honey bees constitutes true detoxification. In the nicotine-resistant peach-potato aphid (M. persicae) overexpression of a cytochrome P450 (CYP6CY3) allows these insects to efficiently detoxify nicotine to cotinine and ...
List of abbreviations
... countries with more than one study, the most recent figures are included. 1 ...
... countries with more than one study, the most recent figures are included. 1 ...
Localization of Low-sulfur Keratin Proteins in the Wool Follicle Using
... Low-sulfur keratin proteins were electrophoresed on 10% polyacrylamide SDS gels according to the method of Laemmli (17) . The proteins were transferred to nitrocellulose membranes (0 .45 pm pore size ; Schleicher & Schull, Inc., West Germany) in a transfer buffer consisting of 0.025 M Tris-HCI, 0 .1 ...
... Low-sulfur keratin proteins were electrophoresed on 10% polyacrylamide SDS gels according to the method of Laemmli (17) . The proteins were transferred to nitrocellulose membranes (0 .45 pm pore size ; Schleicher & Schull, Inc., West Germany) in a transfer buffer consisting of 0.025 M Tris-HCI, 0 .1 ...
Free fatty acids regulate the uncoupling protein and alternative
... two energy-dissipating systems in plants (contrary to heterotrophic organisms) and how they interact to fulfil requirements of plant cell energy metabolism. In this work we describe the functional connection between the AOX and PUMP energy-dissipating systems in mitochondria isolated from green toma ...
... two energy-dissipating systems in plants (contrary to heterotrophic organisms) and how they interact to fulfil requirements of plant cell energy metabolism. In this work we describe the functional connection between the AOX and PUMP energy-dissipating systems in mitochondria isolated from green toma ...
Coordination of the maize transcriptome by a conserved circadian
... with "cellular amino acid and derivative metabolic process" (GO: 0006519), which showed overrepresentation of genes with peak levels at ZT20 (Table 1, Figure 2). These results indicate maize protein synthesis may also have a pre-dawn phase. Interestingly, Arabidopsis genes associated with protein sy ...
... with "cellular amino acid and derivative metabolic process" (GO: 0006519), which showed overrepresentation of genes with peak levels at ZT20 (Table 1, Figure 2). These results indicate maize protein synthesis may also have a pre-dawn phase. Interestingly, Arabidopsis genes associated with protein sy ...
CHEMICAL SYNTHESIS OF PROTEINS : A TOOL FOR PROTEIN
... chemical modifications, such as unnatural amino acids, post-translational modifications, isotopes and fluorophores. A semisynthetic version of NCL is the Expressed Protein Ligation (EPL), in which one or both of the peptide building blocks are made by recombinant DNA expression, while the actual lig ...
... chemical modifications, such as unnatural amino acids, post-translational modifications, isotopes and fluorophores. A semisynthetic version of NCL is the Expressed Protein Ligation (EPL), in which one or both of the peptide building blocks are made by recombinant DNA expression, while the actual lig ...